Restriction map for
pFN21AE1820
(Circular) (Six-base) MAPSORT of: pFN21AE1820.seq Check: 4858 from: 1 to: 5707
pFN21AE1820 5707 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3517 278
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3050 1937
Size: 1113 4594
Acc65I G'GTAC_C
Cuts at: 3028 3028
Size: 5707
AclI AA'CG_TT
Cuts at: 1563 3834 4207 1563
Size: 2271 373 3063
Fragments arranged by size:
3063 2271 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2289 3770 5154 5422 1632
Size: 657 1481 1384 268 1917
Fragments arranged by size:
1917 1481 1384 657 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5510
AflIII A'CryG_T
Cuts at: 1203 4606 5583 1203
Size: 3403 977 1327
Fragments arranged by size:
3403 1327 977
AgeI A'CCGG_T
Cuts at: 4538 4538
Size: 5707
AhdI GACnn_n'nnGTC
Cuts at: 4436 4436
Size: 5707
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5707
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5707
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2544 2576 2544
Size: 32 5675
AlwNI CAG_nnn'CTG
Cuts at: 2267 2548 2686 3450 5022 2267
Size: 281 138 764 1572 2952
Fragments arranged by size:
2952 1572 764 281 138
ApaI G_GGCC'C
Cuts at: 2240 2811 2240
Size: 571 5136
ApaLI G'TGCA_C
Cuts at: 2097 2918 3764 4920 2097
Size: 821 846 1156 2884
Fragments arranged by size:
2884 1156 846 821
ApoI r'AATT_y
Cuts at: 1493 3016 3242 3394 5503 1493
Size: 1523 226 152 2109 1697
Fragments arranged by size:
2109 1697 1523 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4261 160
Size: 872 3229 1606
Fragments arranged by size:
3229 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
AvaI C'yCGr_G
Cuts at: 1870 1943 2804 3032 1870
Size: 73 861 228 4545
Fragments arranged by size:
4545 861 228 73
BamHI G'GATC_C
Cuts at: 2937 3037 3404 2937
Size: 100 367 5240
Fragments arranged by size:
5240 367 100
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2249 2888 3028 4483 618
Size: 325 217 627 462 639 140 1455 1842
Fragments arranged by size:
1842 1455 639 627 462 325 217 140
BanII G_rGCy'C
Cuts at: 1391 2240 2283 2811 2832 3026 1391
Size: 849 43 528 21 194 4072
Fragments arranged by size:
4072 849 528 194 43 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2295 2408 928
Size: 971 396 113 4227
Fragments arranged by size:
4227 971 396 113
BbvCI CC'TCA_GC
Cuts at: 2297 2297
Size: 5707
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3898 3932 1903
Size: 34 1961 34 3678
Fragments arranged by size:
3678 1961 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3599 4809 3599
Size: 1210 4497
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5707
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2020 2258 2843
Size: 107 122 71 631 605 348 238 585
Cuts at: 2843 4318 136
Size: 1475 1525
Fragments arranged by size:
1525 1475 631 605 585 348 238 122
107 71
BglII A'GATC_T
Cuts at: 5702 5702
Size: 5707
BlpI GC'TnA_GC
Cuts at: 2861 3124 2861
Size: 263 5444
Bme1580I G_kGCm'C
Cuts at: 1790 2101 2240 2811 2891 2922 3768 4924 1790
Size: 311 139 571 80 31 846 1156 2573
Fragments arranged by size:
2573 1156 846 571 311 139 80 31
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5707
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2141 3438 4396
Size: 416 53 83 399 24 724 1297 958
Cuts at: 4396 442
Size: 1753
Fragments arranged by size:
1753 1297 958 724 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2502 2675 4367 1239
Size: 1263 173 1692 2579
Fragments arranged by size:
2579 1692 1263 173
Bpu10I CC'TnA_GC
Cuts at: 2297 2396 2297
Size: 99 5608
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3832 4697 4995 3832
Size: 865 298 4544
Fragments arranged by size:
4544 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2197 2346 4370 882
Size: 617 698 149 2024 2219
Fragments arranged by size:
2219 2024 698 617 149
BsaAI yAC'GTr
Cuts at: 493 1204 2917 3498 493
Size: 711 1713 581 2702
Fragments arranged by size:
2702 1713 711 581
BsaBI GATnn'nnATC
Cuts at: 5701 5701
Size: 5707
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3514 3896 4531 275
Size: 53 83 186 936 1981 382 635 1451
Fragments arranged by size:
1981 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4140 4538 4812 4959 5370 4140
Size: 398 274 147 411 4477
Fragments arranged by size:
4477 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5482
Fragments arranged by size:
5482 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2215 2236 2451 2672 2675 1186
Size: 1029 21 215 221 3 4218
Fragments arranged by size:
4218 1029 221 215 21 3
BseYI C'CCAG_C
Cuts at: 2018 2070 2102 2124 2275 2509 2541 2585 2881
Size: 52 32 22 151 234 32 44 296
Cuts at: 2881 2955 4910 5406 2018
Size: 74 1955 496 2319
Fragments arranged by size:
2319 1955 496 296 234 151 74 52
44 32 32 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5134
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3157 3918 4946 1540
Size: 275 186 1156 761 1028 2301
Fragments arranged by size:
2301 1156 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2101 2916 2922 3768 3853 4924 2101
Size: 815 6 846 85 1071 2884
Fragments arranged by size:
2884 1071 846 815 85 6
BsmI GAATG_Cn'
Cuts at: 3218 3311 3218
Size: 93 5614
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2101 2240 2283 2811 2832 2891 2916
Size: 399 311 139 43 528 21 59 25
Cuts at: 2916 2922 3026 3768 3853 4924 1391
Size: 6 104 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 528 399 311 139 104
85 59 43 25 21 6
BspEI T'CCGG_A
Cuts at: 5370 5370
Size: 5707
BspHI T'CATG_A
Cuts at: 3596 5326 3596
Size: 1730 3977
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3062 844
Size: 2218 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3594 1394
Size: 429 1771 3507
Fragments arranged by size:
3507 1771 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4202 4376 65
Size: 1183 2954 174 1396
Fragments arranged by size:
2954 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2129 2865 4351 4538 1700
Size: 110 319 736 1486 187 2869
Fragments arranged by size:
2869 1486 736 319 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5707
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5707
BssSI C'ACGA_G
Cuts at: 3767 4779 3767
Size: 1012 4695
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5707
BstBI TT'CG_AA
Cuts at: 2407 4511 2407
Size: 2104 3603
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5707
BstYI r'GATC_y
Cuts at: 1972 2937 3037 3404 3789 3806 5258 5344 5356
Size: 965 100 367 385 17 1452 86 12
Cuts at: 5356 5702 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 965 385 367 346 100 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2318 2318
Size: 5707
BtgI C'CryG_G
Cuts at: 513 1065 2004 2256 2853 2947 2982 513
Size: 552 939 252 597 94 35 3238
Fragments arranged by size:
3238 939 597 552 252 94 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4968
Fragments arranged by size:
4968 683 56
BtsI GCAGTG_nn'
Cuts at: 3223 4017 4037 3223
Size: 794 20 4893
Fragments arranged by size:
4893 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5707
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2198 2233 529
Size: 35 1634 35 4003
Fragments arranged by size:
4003 1634 35 35
DraI TTT'AAA
Cuts at: 3011 3011
Size: 5707
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4714 809
Size: 507 3398 1802
Fragments arranged by size:
3398 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2057 2874 2895
Size: 54 1425 50 275 18 227 817 21
Cuts at: 2895 3154 4043 5672 8
Size: 259 889 1629 43
Fragments arranged by size:
1629 1425 889 817 275 259 227 54
50 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 3154 1537
Size: 275 1342 4090
Fragments arranged by size:
4090 1342 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2265 2373 3637 1353
Size: 912 108 1264 3423
Fragments arranged by size:
3423 1264 912 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2100 2471 2571 2711 4294 4668
Size: 1064 138 511 371 100 140 1583 374
Cuts at: 4668 4814 387
Size: 146 1280
Fragments arranged by size:
1583 1280 1064 511 374 371 146 140
138 100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2289 2502 2675 3770 4367 5154 5422
Size: 393 657 213 173 1095 597 787 268
Cuts at: 5422 1239
Size: 1524
Fragments arranged by size:
1524 1095 787 657 597 393 268 213
173
EcoNI CCTnn'n_nnAGG
Cuts at: 2317 2654 2317
Size: 337 5370
EcoO109I rG'GnC_Cy
Cuts at: 2236 2237 2302 2603 2808 2236
Size: 1 65 301 205 5135
Fragments arranged by size:
5135 301 205 65 1
EcoRI G'AATT_C
Cuts at: 3016 3394 3016
Size: 378 5329
FspI TGC'GCA
Cuts at: 2153 2708 4213 2153
Size: 555 1505 3647
Fragments arranged by size:
3647 1505 555
HaeII r_GCGC'y
Cuts at: 1678 4854 1678
Size: 3176 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2765 2797 4348 4380 4422
Size: 32 135 32 1193 32 1551 32 42
Cuts at: 4422 4454 1373
Size: 32 2626
Fragments arranged by size:
2626 1551 1193 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3051 3297 669
Size: 988 281 1113 246 3079
Fragments arranged by size:
3079 1113 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5707
HpaI GTT'AAC
Cuts at: 3297 3297
Size: 5707
KpnI G_GTAC'C
Cuts at: 3032 3032
Size: 5707
MfeI C'AATT_G
Cuts at: 3306 3306
Size: 5707
MluI A'CGCG_T
Cuts at: 5583 5583
Size: 5707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2375 2757 4821 5005 1305
Size: 1070 382 2064 184 2007
Fragments arranged by size:
2064 2007 1070 382 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3885
Fragments arranged by size:
3885 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3665 4024 4183 518
Size: 648 2499 359 159 2042
Fragments arranged by size:
2499 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2018 2545 2557 2572 3123 3800 4948 5193
Size: 412 527 12 15 551 677 1148 245
Cuts at: 5193 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 551 527 412 245 15
12
NaeI GCC'GGC
Cuts at: 2867 2867
Size: 5707
NcoI C'CATG_G
Cuts at: 513 1065 2004 2256 2853 513
Size: 552 939 252 597 3367
Fragments arranged by size:
3367 939 597 552 252
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5707
NgoMIV G'CCGG_C
Cuts at: 2865 2865
Size: 5707
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5707
NotI GC'GGCC_GC
Cuts at: 3154 3154
Size: 5707
NspI r_CATG'y
Cuts at: 1132 1229 2070 2086 2802 3065 4610 1132
Size: 97 841 16 716 263 1545 2229
Fragments arranged by size:
2229 1545 841 716 263 97 16
PasI CC'CwG_GG
Cuts at: 1348 2428 1348
Size: 1080 4627
PciI A'CATG_T
Cuts at: 4606 4606
Size: 5707
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2548 2686 1108
Size: 1440 138 4129
Fragments arranged by size:
4129 1440 138
PfoI T'CCnGG_A
Cuts at: 2283 2283
Size: 5707
PmeI GTTT'AAAC
Cuts at: 3011 3011
Size: 5707
PmlI CAC'GTG
Cuts at: 2917 2917
Size: 5707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3776 3808 5289 5321 3776
Size: 32 1481 32 4162
Fragments arranged by size:
4162 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2302 2603 2302
Size: 301 5406
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5707
PsiI TTA'TAA
Cuts at: 3277 3277
Size: 5707
PspOMI G'GGCC_C
Cuts at: 2236 2807 2236
Size: 571 5136
PstI C_TGCA'G
Cuts at: 830 3059 830
Size: 2229 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5707
PvuII CAG'CTG
Cuts at: 2557 2572 2557
Size: 15 5692
SalI G'TCGA_C
Cuts at: 1936 3049 1936
Size: 1113 4594
SanDI GG'GwC_CC
Cuts at: 2603 2603
Size: 5707
SapI GCTCTTCn'nnn_
Cuts at: 2265 2373 2265
Size: 108 5599
SbfI CC_TGCA'GG
Cuts at: 3059 3059
Size: 5707
ScaI AGT'ACT
Cuts at: 3955 5667 3955
Size: 1712 3995
SexAI A'CCwGG_T
Cuts at: 2245 2245
Size: 5707
SfcI C'TryA_G
Cuts at: 826 1045 2764 3055 4190 4871 5062 826
Size: 219 1719 291 1135 681 191 1471
Fragments arranged by size:
1719 1471 1135 681 291 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2843 2843
Size: 5707
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5707
SmaI CCC'GGG
Cuts at: 1872 2806 3034 1872
Size: 934 228 4545
Fragments arranged by size:
4545 934 228
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3811 4712 4974 820
Size: 197 926 1868 901 262 1553
Fragments arranged by size:
1868 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3498 493
Size: 3005 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5707
SphI G_CATG'C
Cuts at: 1132 2070 2802 3065 1132
Size: 938 732 263 3774
Fragments arranged by size:
3774 938 732 263
SspI AAT'ATT
Cuts at: 5 52 3631 5
Size: 47 3579 2081
Fragments arranged by size:
3579 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5707
StyI C'CwwG_G
Cuts at: 513 1065 2004 2256 2853 3146 513
Size: 552 939 252 597 293 3074
Fragments arranged by size:
3074 939 597 552 293 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2116 3734 3751 3904 4089 4534 325
Size: 1255 536 1618 17 153 185 445 1498
Fragments arranged by size:
1618 1498 1255 536 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3953 5665 96
Size: 274 80 33 51 1443 1976 1712 138
Fragments arranged by size:
1976 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2892 2924 5667 5699 2892
Size: 32 2743 32 2900
Fragments arranged by size:
2900 2743 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5707
XbaI T'CTAG_A
Cuts at: 3043 3043
Size: 5707
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2067 2067
Size: 5707
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5707
XmaI C'CCGG_G
Cuts at: 1870 2804 3032 1870
Size: 934 228 4545
Fragments arranged by size:
4545 934 228
XmnI GAAnn'nnTTC
Cuts at: 3836 3836
Size: 5707
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3515 276
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PspXI
PsrI RsrII SacI SacII SfoI SrfI SwaI