Restriction map for
pFN21AE1813
(Circular) (Six-base) MAPSORT of: pFN21AE1813.seq Check: 6944 from: 1 to: 5692
pFN21AE1813 5692 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3502 278
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2361 3035 1937
Size: 424 674 4594
Fragments arranged by size:
4594 674 424
Acc65I G'GTAC_C
Cuts at: 3013 3013
Size: 5692
AclI AA'CG_TT
Cuts at: 1563 3819 4192 1563
Size: 2256 373 3063
Fragments arranged by size:
3063 2256 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2186 2616 2729 3755 5139 5407 1632
Size: 554 430 113 1026 1384 268 1917
Fragments arranged by size:
1917 1384 1026 554 430 268 113
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5495
AflIII A'CryG_T
Cuts at: 1203 4591 5568 1203
Size: 3388 977 1327
Fragments arranged by size:
3388 1327 977
AgeI A'CCGG_T
Cuts at: 4523 4523
Size: 5692
AhdI GACnn_n'nnGTC
Cuts at: 4421 4421
Size: 5692
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5692
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2680 1184
Size: 1496 4196
AlwNI CAG_nnn'CTG
Cuts at: 2357 3435 5007 2357
Size: 1078 1572 3042
Fragments arranged by size:
3042 1572 1078
ApaLI G'TGCA_C
Cuts at: 3749 4905 3749
Size: 1156 4536
ApoI r'AATT_y
Cuts at: 1493 3001 3227 3379 5488 1493
Size: 1508 226 152 2109 1697
Fragments arranged by size:
2109 1697 1508 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4246 160
Size: 872 3214 1606
Fragments arranged by size:
3214 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
AvaI C'yCGr_G
Cuts at: 1870 1943 2687 2724 3017 1870
Size: 73 744 37 293 4545
Fragments arranged by size:
4545 744 293 73 37
BamHI G'GATC_C
Cuts at: 3022 3389 3022
Size: 367 5325
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2441 3013 4468 618
Size: 325 217 627 654 572 1455 1842
Fragments arranged by size:
1842 1455 654 627 572 325 217
BanII G_rGCy'C
Cuts at: 1391 2016 2350 3011 1391
Size: 625 334 661 4072
Fragments arranged by size:
4072 661 625 334
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2102 928
Size: 971 203 4518
Fragments arranged by size:
4518 971 203
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3883 3917 1903
Size: 34 1946 34 3678
Fragments arranged by size:
3678 1946 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3584 4794 3584
Size: 1210 4482
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5692
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2972 4303 136
Size: 107 122 71 631 605 1300 1331 1525
Fragments arranged by size:
1525 1331 1300 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5687 5687
Size: 5692
BlpI GC'TnA_GC
Cuts at: 3109 3109
Size: 5692
Bme1580I G_kGCm'C
Cuts at: 1790 2446 3753 4909 1790
Size: 656 1307 1156 2573
Fragments arranged by size:
2573 1307 1156 656
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5692
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2089 2917 3423
Size: 416 53 83 399 24 672 828 506
Cuts at: 3423 4381 442
Size: 958 1753
Fragments arranged by size:
1753 958 828 672 506 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5692
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2064 2064
Size: 5692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2468 4352 1239
Size: 1229 1884 2579
Fragments arranged by size:
2579 1884 1229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2000 2210 2444 3817 4682 4980 2000
Size: 210 234 1373 865 298 2712
Fragments arranged by size:
2712 1373 865 298 234 210
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4355 882
Size: 617 2856 2219
Fragments arranged by size:
2856 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3483 493
Size: 711 2279 2702
Fragments arranged by size:
2702 2279 711
BsaBI GATnn'nnATC
Cuts at: 5686 5686
Size: 5692
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3499 3881 4516 275
Size: 53 83 186 936 1966 382 635 1451
Fragments arranged by size:
1966 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4125 4523 4797 4944 5355 4125
Size: 398 274 147 411 4462
Fragments arranged by size:
4462 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5467
Fragments arranged by size:
5467 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 1186
Size: 889 4803
BseYI C'CCAG_C
Cuts at: 2895 4895 5391 2895
Size: 2000 496 3196
Fragments arranged by size:
3196 2000 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2591 1163
Size: 573 855 4264
Fragments arranged by size:
4264 855 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3142 3903 4931 1540
Size: 275 186 1141 761 1028 2301
Fragments arranged by size:
2301 1141 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3753 3838 4909 3753
Size: 85 1071 4536
Fragments arranged by size:
4536 1071 85
BsmI GAATG_Cn'
Cuts at: 3203 3296 3203
Size: 93 5599
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2350 2446 3011 3753 3838 4909
Size: 399 226 334 96 565 742 85 1071
Cuts at: 4909 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 565 399 334 226 96
85
BspEI T'CCGG_A
Cuts at: 5355 5355
Size: 5692
BspHI T'CATG_A
Cuts at: 3581 5311 3581
Size: 1730 3962
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3047 844
Size: 2203 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3579 1394
Size: 429 1756 3507
Fragments arranged by size:
3507 1756 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2961 4187 4361 65
Size: 1183 1713 1226 174 1396
Fragments arranged by size:
1713 1396 1226 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4336 4523 1700
Size: 110 2526 187 2869
Fragments arranged by size:
2869 2526 187 110
BsrGI T'GTAC_A
Cuts at: 96 2637 96
Size: 2541 3151
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5692
BssSI C'ACGA_G
Cuts at: 3752 4764 3752
Size: 1012 4680
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5692
BstBI TT'CG_AA
Cuts at: 4496 4496
Size: 5692
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5692
BstYI r'GATC_y
Cuts at: 1972 2682 3022 3389 3774 3791 5243 5329 5341
Size: 710 340 367 385 17 1452 86 12
Cuts at: 5341 5687 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 710 385 367 346 340 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2058 2058
Size: 5692
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5140
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4953
Fragments arranged by size:
4953 683 56
BtsI GCAGTG_nn'
Cuts at: 2724 2850 3208 4002 4022 2724
Size: 126 358 794 20 4394
Fragments arranged by size:
4394 794 358 126 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5692
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2938 2973 529
Size: 35 2374 35 3248
Fragments arranged by size:
3248 2374 35 35
DraI TTT'AAA
Cuts at: 2996 2996
Size: 5692
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4699 809
Size: 507 3383 1802
Fragments arranged by size:
3383 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2745 2973 3139
Size: 54 1425 50 275 18 915 228 166
Cuts at: 3139 4028 5657 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 915 889 275 228 166 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3139 1537
Size: 275 1327 4090
Fragments arranged by size:
4090 1327 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2250 2619 2705 3622 1353
Size: 897 369 86 917 3423
Fragments arranged by size:
3423 917 897 369 86
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4279 4653 4799 387
Size: 1064 138 2690 374 146 1280
Fragments arranged by size:
2690 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2186 2468 2616 2729 3755 4352 5139
Size: 393 554 282 148 113 1026 597 787
Cuts at: 5139 5407 1239
Size: 268 1524
Fragments arranged by size:
1524 1026 787 597 554 393 282 268
148 113
EcoO109I rG'GnC_Cy
Cuts at: 2732 2732
Size: 5692
EcoRI G'AATT_C
Cuts at: 3001 3379 3001
Size: 378 5314
FspI TGC'GCA
Cuts at: 4198 4198
Size: 5692
HaeII r_GCGC'y
Cuts at: 1678 4839 1678
Size: 3161 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2120 2152 2501 2533 2841
Size: 32 135 32 548 32 349 32 308
Cuts at: 2841 2873 2913 2945 4333 4365 4407 4439 1373
Size: 32 40 32 1388 32 42 32 2626
Fragments arranged by size:
2626 1388 548 349 308 135 42 40
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2362 3036 3282 669
Size: 988 281 424 674 246 3079
Fragments arranged by size:
3079 988 674 424 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5692
HpaI GTT'AAC
Cuts at: 3282 3282
Size: 5692
KpnI G_GTAC'C
Cuts at: 3017 3017
Size: 5692
MfeI C'AATT_G
Cuts at: 3291 3291
Size: 5692
MluI A'CGCG_T
Cuts at: 5568 5568
Size: 5692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4806 4990 1305
Size: 3501 184 2007
Fragments arranged by size:
3501 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2747 2975 10
Size: 54 1425 343 915 228 2727
Fragments arranged by size:
2727 1425 915 343 228 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2214 3650 4009 4168 518
Size: 648 1048 1436 359 159 2042
Fragments arranged by size:
2042 1436 1048 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2895 3108 3785 4933 5178 1606
Size: 1289 213 677 1148 245 2120
Fragments arranged by size:
2120 1289 1148 677 245 213
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5140
NdeI CA'TA_TG
Cuts at: 387 2215 2611 387
Size: 1828 396 3468
Fragments arranged by size:
3468 1828 396
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5692
NotI GC'GGCC_GC
Cuts at: 3139 3139
Size: 5692
NspI r_CATG'y
Cuts at: 1132 1229 3050 4595 1132
Size: 97 1821 1545 2229
Fragments arranged by size:
2229 1821 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5692
PciI A'CATG_T
Cuts at: 4591 4591
Size: 5692
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5692
PmeI GTTT'AAAC
Cuts at: 2996 2996
Size: 5692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3761 3793 5274 5306 3761
Size: 32 1481 32 4147
Fragments arranged by size:
4147 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2732 2732
Size: 5692
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5692
PsiI TTA'TAA
Cuts at: 3262 3262
Size: 5692
PstI C_TGCA'G
Cuts at: 830 3044 830
Size: 2214 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5692
PvuII CAG'CTG
Cuts at: 2895 2895
Size: 5692
SalI G'TCGA_C
Cuts at: 1936 2360 3034 1936
Size: 424 674 4594
Fragments arranged by size:
4594 674 424
SbfI CC_TGCA'GG
Cuts at: 3044 3044
Size: 5692
ScaI AGT'ACT
Cuts at: 3940 5652 3940
Size: 1712 3980
SfcI C'TryA_G
Cuts at: 826 1045 3040 4175 4856 5047 826
Size: 219 1995 1135 681 191 1471
Fragments arranged by size:
1995 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5692
SmaI CCC'GGG
Cuts at: 1872 3019 1872
Size: 1147 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2015 2189 2459 3796 4697 4959
Size: 197 926 72 174 270 1337 901 262
Cuts at: 4959 820
Size: 1553
Fragments arranged by size:
1553 1337 926 901 270 262 197 174
72
SnaBI TAC'GTA
Cuts at: 493 3483 493
Size: 2990 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5692
SphI G_CATG'C
Cuts at: 1132 3050 1132
Size: 1918 3774
SspI AAT'ATT
Cuts at: 5 52 3616 5
Size: 47 3564 2081
Fragments arranged by size:
3564 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5692
StyI C'CwwG_G
Cuts at: 513 1065 3131 513
Size: 552 2066 3074
Fragments arranged by size:
3074 2066 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2090 2429 3719 3736 3889 4074 4519
Size: 1255 510 339 1290 17 153 185 445
Cuts at: 4519 325
Size: 1498
Fragments arranged by size:
1498 1290 1255 510 445 339 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2637 3938 5650
Size: 274 80 33 51 1443 660 1301 1712
Cuts at: 5650 96
Size: 138
Fragments arranged by size:
1712 1443 1301 660 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5652 5684 5652
Size: 32 5660
Tth111I GACn'n_nGTC
Cuts at: 1316 2357 1316
Size: 1041 4651
XbaI T'CTAG_A
Cuts at: 3028 3028
Size: 5692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2257 2257
Size: 5692
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5692
XmaI C'CCGG_G
Cuts at: 1870 3017 1870
Size: 1147 4545
XmnI GAAnn'nnTTC
Cuts at: 3821 3821
Size: 5692
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3500 276
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI