Restriction map for
pFN21AE1812
(Circular) (Six-base) MAPSORT of: pFN21AE1812.seq Check: 8166 from: 1 to: 5689
pFN21AE1812 5689 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2435 2746 2435
Size: 311 5378
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3499 278
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3032 1937
Size: 1095 4594
Acc65I G'GTAC_C
Cuts at: 3010 3010
Size: 5689
AclI AA'CG_TT
Cuts at: 1563 3816 4189 1563
Size: 2253 373 3063
Fragments arranged by size:
3063 2253 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2102 2184 2753 3752 5136 5404 1632
Size: 470 82 569 999 1384 268 1917
Fragments arranged by size:
1917 1384 999 569 470 268 82
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5492
AflIII A'CryG_T
Cuts at: 1203 4588 5565 1203
Size: 3385 977 1327
Fragments arranged by size:
3385 1327 977
AgeI A'CCGG_T
Cuts at: 4520 4520
Size: 5689
AhdI GACnn_n'nnGTC
Cuts at: 4418 4418
Size: 5689
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5689
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2942 1184
Size: 1758 3931
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2274 2306 2274
Size: 32 5657
AlwNI CAG_nnn'CTG
Cuts at: 2598 2609 3432 5004 2598
Size: 11 823 1572 3283
Fragments arranged by size:
3283 1572 823 11
ApaI G_GGCC'C
Cuts at: 2935 2935
Size: 5689
ApaLI G'TGCA_C
Cuts at: 3746 4902 3746
Size: 1156 4533
ApoI r'AATT_y
Cuts at: 1493 2334 2746 2998 3224 3376 5485 1493
Size: 841 412 252 226 152 2109 1697
Fragments arranged by size:
2109 1697 841 412 252 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4243 160
Size: 872 3211 1606
Fragments arranged by size:
3211 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
AvaI C'yCGr_G
Cuts at: 1870 1943 3014 1870
Size: 73 1071 4545
Fragments arranged by size:
4545 1071 73
BamHI G'GATC_C
Cuts at: 3019 3386 3019
Size: 367 5322
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3010 4465 618
Size: 325 217 627 1223 1455 1842
Fragments arranged by size:
1842 1455 1223 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2935 3008 1391
Size: 1544 73 4072
Fragments arranged by size:
4072 1544 73
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2863 928
Size: 971 964 3754
Fragments arranged by size:
3754 971 964
BbvCI CC'TCA_GC
Cuts at: 2610 2887 2610
Size: 277 5412
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3880 3914 1903
Size: 34 1943 34 3678
Fragments arranged by size:
3678 1943 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2127 3581 4791 2127
Size: 1454 1210 3025
Fragments arranged by size:
3025 1454 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5689
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4300 136
Size: 107 122 71 631 605 2628 1525
Fragments arranged by size:
2628 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5684 5684
Size: 5689
BlpI GC'TnA_GC
Cuts at: 3106 3106
Size: 5689
Bme1580I G_kGCm'C
Cuts at: 1790 2935 2982 3750 4906 1790
Size: 1145 47 768 1156 2573
Fragments arranged by size:
2573 1156 1145 768 47
BmgBI CAC'GTC
Cuts at: 1318 2511 1318
Size: 1193 4496
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3420 4378 442
Size: 416 53 83 399 24 2003 958 1753
Fragments arranged by size:
2003 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2151 2809 4349 1239
Size: 912 658 1540 2579
Fragments arranged by size:
2579 1540 912 658
Bpu10I CC'TnA_GC
Cuts at: 2610 2887 2610
Size: 277 5412
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2642 3814 4679 4977 2642
Size: 1172 865 298 3354
Fragments arranged by size:
3354 1172 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4352 882
Size: 617 2853 2219
Fragments arranged by size:
2853 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3480 493
Size: 711 2276 2702
Fragments arranged by size:
2702 2276 711
BsaBI GATnn'nnATC
Cuts at: 2115 5683 2115
Size: 3568 2121
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3496 3878 4513 275
Size: 53 83 186 936 1963 382 635 1451
Fragments arranged by size:
1963 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2119 4122 4520 4794 4941 5352 2119
Size: 2003 398 274 147 411 2456
Fragments arranged by size:
2456 2003 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2124 2154 2274 2304 2552
Size: 30 165 30 721 30 120 30 248
Cuts at: 2552 2582 1178
Size: 30 4285
Fragments arranged by size:
4285 721 248 165 120 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2627 1186
Size: 1441 4248
BseYI C'CCAG_C
Cuts at: 2602 2769 4892 5388 2602
Size: 167 2123 496 2903
Fragments arranged by size:
2903 2123 496 167
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5116
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3139 3900 4928 1540
Size: 275 186 1138 761 1028 2301
Fragments arranged by size:
2301 1138 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3750 3835 4906 3750
Size: 85 1071 4533
Fragments arranged by size:
4533 1071 85
BsmI GAATG_Cn'
Cuts at: 3200 3293 3200
Size: 93 5596
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2935 2982 3008 3750 3835 4906 1391
Size: 399 1145 47 26 742 85 1071 2174
Fragments arranged by size:
2174 1145 1071 742 399 85 47 26
BspEI T'CCGG_A
Cuts at: 2119 5352 2119
Size: 3233 2456
BspHI T'CATG_A
Cuts at: 3578 5308 3578
Size: 1730 3959
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2435 2746 3044 844
Size: 1591 311 298 3489
Fragments arranged by size:
3489 1591 311 298
BsrBI CCG'CTC
Cuts at: 1394 1823 3576 1394
Size: 429 1753 3507
Fragments arranged by size:
3507 1753 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4184 4358 65
Size: 1183 2936 174 1396
Fragments arranged by size:
2936 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4333 4520 1700
Size: 110 2523 187 2869
Fragments arranged by size:
2869 2523 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5689
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5689
BssSI C'ACGA_G
Cuts at: 2063 3749 4761 2063
Size: 1686 1012 2991
Fragments arranged by size:
2991 1686 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5689
BstBI TT'CG_AA
Cuts at: 4493 4493
Size: 5689
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5689
BstYI r'GATC_y
Cuts at: 1972 3019 3386 3771 3788 5240 5326 5338 5684
Size: 1047 367 385 17 1452 86 12 346
Cuts at: 5684 1972
Size: 1977
Fragments arranged by size:
1977 1452 1047 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2892 513
Size: 552 1827 3310
Fragments arranged by size:
3310 1827 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4950
Fragments arranged by size:
4950 683 56
BtsI GCAGTG_nn'
Cuts at: 2206 2992 3205 3999 4019 2206
Size: 786 213 794 20 3876
Fragments arranged by size:
3876 794 786 213 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5689
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5654
DraI TTT'AAA
Cuts at: 2355 2993 2355
Size: 638 5051
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4696 809
Size: 507 3380 1802
Fragments arranged by size:
3380 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2067 2260 3136
Size: 54 1425 50 275 18 237 193 876
Cuts at: 3136 4025 5654 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 876 275 237 193 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3136 1537
Size: 275 1324 4090
Fragments arranged by size:
4090 1324 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2494 3619 1353
Size: 1141 1125 3423
Fragments arranged by size:
3423 1141 1125
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4276 4650 4796 387
Size: 1064 138 2687 374 146 1280
Fragments arranged by size:
2687 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2151 2184 2753 2809 3752 4349
Size: 393 470 49 33 569 56 943 597
Cuts at: 4349 5136 5404 1239
Size: 787 268 1524
Fragments arranged by size:
1524 943 787 597 569 470 393 268
56 49 33
EcoRI G'AATT_C
Cuts at: 2746 2998 3376 2746
Size: 252 378 5059
Fragments arranged by size:
5059 378 252
FspI TGC'GCA
Cuts at: 4195 4195
Size: 5689
HaeII r_GCGC'y
Cuts at: 1678 4836 1678
Size: 3158 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2870 2902 4330 4362 4404
Size: 32 135 32 1298 32 1428 32 42
Cuts at: 4404 4436 1373
Size: 32 2626
Fragments arranged by size:
2626 1428 1298 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3033 3279 669
Size: 988 281 1095 246 3079
Fragments arranged by size:
3079 1095 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5689
HpaI GTT'AAC
Cuts at: 3279 3279
Size: 5689
KpnI G_GTAC'C
Cuts at: 3014 3014
Size: 5689
MfeI C'AATT_G
Cuts at: 3288 3288
Size: 5689
MluI A'CGCG_T
Cuts at: 5565 5565
Size: 5689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4803 4987 1305
Size: 3498 184 2007
Fragments arranged by size:
3498 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2069 2262 10
Size: 54 1425 343 237 193 3437
Fragments arranged by size:
3437 1425 343 237 193 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2924 3647 4006 4165 518
Size: 648 1758 723 359 159 2042
Fragments arranged by size:
2042 1758 723 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2606 2731 2769 3105 3782 4930 5175 1606
Size: 1000 125 38 336 677 1148 245 2120
Fragments arranged by size:
2120 1148 1000 677 336 245 125 38
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5137
NdeI CA'TA_TG
Cuts at: 387 2072 387
Size: 1685 4004
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5689
NotI GC'GGCC_GC
Cuts at: 3136 3136
Size: 5689
NspI r_CATG'y
Cuts at: 1132 1229 3047 4592 1132
Size: 97 1818 1545 2229
Fragments arranged by size:
2229 1818 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5689
PciI A'CATG_T
Cuts at: 4588 4588
Size: 5689
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2020 1108
Size: 912 4777
PmeI GTTT'AAAC
Cuts at: 2993 2993
Size: 5689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2274 2306 3758 3790 5271 5303 2274
Size: 32 1452 32 1481 32 2660
Fragments arranged by size:
2660 1481 1452 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5689
PsiI TTA'TAA
Cuts at: 3259 3259
Size: 5689
PspOMI G'GGCC_C
Cuts at: 2931 2931
Size: 5689
PstI C_TGCA'G
Cuts at: 830 2536 2987 3041 830
Size: 1706 451 54 3478
Fragments arranged by size:
3478 1706 451 54
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5689
PvuII CAG'CTG
Cuts at: 2606 2731 2769 2606
Size: 125 38 5526
Fragments arranged by size:
5526 125 38
SalI G'TCGA_C
Cuts at: 1936 3031 1936
Size: 1095 4594
SapI GCTCTTCn'nnn_
Cuts at: 2494 2494
Size: 5689
SbfI CC_TGCA'GG
Cuts at: 3041 3041
Size: 5689
ScaI AGT'ACT
Cuts at: 2452 2570 2794 3937 5649 2452
Size: 118 224 1143 1712 2492
Fragments arranged by size:
2492 1712 1143 224 118
SfcI C'TryA_G
Cuts at: 826 1045 2532 2947 2983 3037 4172 4853 5044
Size: 219 1487 415 36 54 1135 681 191
Cuts at: 5044 826
Size: 1471
Fragments arranged by size:
1487 1471 1135 681 415 219 191 54
36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5689
SmaI CCC'GGG
Cuts at: 1872 3016 1872
Size: 1144 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2657 3793 4694 4956 820
Size: 197 926 714 1136 901 262 1553
Fragments arranged by size:
1553 1136 926 901 714 262 197
SnaBI TAC'GTA
Cuts at: 493 3480 493
Size: 2987 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5689
SphI G_CATG'C
Cuts at: 1132 3047 1132
Size: 1915 3774
SspI AAT'ATT
Cuts at: 5 52 3613 5
Size: 47 3561 2081
Fragments arranged by size:
3561 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5689
StyI C'CwwG_G
Cuts at: 513 1065 2187 2500 3128 513
Size: 552 1122 313 628 3074
Fragments arranged by size:
3074 1122 628 552 313
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2731 3716 3733 3886 4071 4516 325
Size: 1255 1151 985 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1151 985 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2265 2450 2568
Size: 274 80 33 51 1443 288 185 118
Cuts at: 2568 2792 3935 5647 96
Size: 224 1143 1712 138
Fragments arranged by size:
1712 1443 1143 288 274 224 185 138
118 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2124 2156 5649 5681 2124
Size: 32 3493 32 2132
Fragments arranged by size:
3493 2132 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5689
XbaI T'CTAG_A
Cuts at: 3025 3025
Size: 5689
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2632 2632
Size: 5689
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5689
XmaI C'CCGG_G
Cuts at: 1870 3014 1870
Size: 1144 4545
XmnI GAAnn'nnTTC
Cuts at: 3818 3818
Size: 5689
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3497 276
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI