Restriction map for
pFN21AE1809
(Circular) (Six-base) MAPSORT of: pFN21AE1809.seq Check: 9454 from: 1 to: 5680
pFN21AE1809 5680 bp
With 182 enzymes: SgfI *
January 30, 2014 17:20 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3490 278
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3023 1937
Size: 1086 4594
Acc65I G'GTAC_C
Cuts at: 3001 3001
Size: 5680
AclI AA'CG_TT
Cuts at: 1563 3807 4180 1563
Size: 2244 373 3063
Fragments arranged by size:
3063 2244 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2880 3743 5127 5395 1632
Size: 1248 863 1384 268 1917
Fragments arranged by size:
1917 1384 1248 863 268
AfeI AGC'GCT
Cuts at: 2390 2390
Size: 5680
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5483
AflIII A'CryG_T
Cuts at: 1203 2010 4579 5556 1203
Size: 807 2569 977 1327
Fragments arranged by size:
2569 1327 977 807
AgeI A'CCGG_T
Cuts at: 4511 4511
Size: 5680
AhdI GACnn_n'nnGTC
Cuts at: 4409 4409
Size: 5680
AleI CACnn'nnGTG
Cuts at: 1166 2234 1166
Size: 1068 4612
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5680
AlwNI CAG_nnn'CTG
Cuts at: 2185 2743 3423 4995 2185
Size: 558 680 1572 2870
Fragments arranged by size:
2870 1572 680 558
ApaLI G'TGCA_C
Cuts at: 3737 4893 3737
Size: 1156 4524
ApoI r'AATT_y
Cuts at: 1493 2788 2989 3215 3367 5476 1493
Size: 1295 201 226 152 2109 1697
Fragments arranged by size:
2109 1697 1295 226 201 152
AseI AT'TA_AT
Cuts at: 160 1032 4234 160
Size: 872 3202 1606
Fragments arranged by size:
3202 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
AvaI C'yCGr_G
Cuts at: 1870 1943 2139 2529 2932 3005 1870
Size: 73 196 390 403 73 4545
Fragments arranged by size:
4545 403 390 196 73 73
BamHI G'GATC_C
Cuts at: 3010 3377 3010
Size: 367 5313
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2066 2811 3001 4456 618
Size: 325 217 627 279 745 190 1455 1842
Fragments arranged by size:
1842 1455 745 627 325 279 217 190
BanII G_rGCy'C
Cuts at: 1391 2999 1391
Size: 1608 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2868 928
Size: 971 969 3740
Fragments arranged by size:
3740 971 969
BbvCI CC'TCA_GC
Cuts at: 2507 2507
Size: 5680
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3871 3905 1903
Size: 34 1934 34 3678
Fragments arranged by size:
3678 1934 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3572 4782 3572
Size: 1210 4470
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5680
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2524 4291 136
Size: 107 122 71 631 605 852 1767 1525
Fragments arranged by size:
1767 1525 852 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2379 5675 2379
Size: 3296 2384
BlpI GC'TnA_GC
Cuts at: 3097 3097
Size: 5680
Bme1580I G_kGCm'C
Cuts at: 1790 2313 3741 4897 1790
Size: 523 1428 1156 2573
Fragments arranged by size:
2573 1428 1156 523
BmgBI CAC'GTC
Cuts at: 1318 2536 1318
Size: 1218 4462
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2113 2879 3411
Size: 416 53 83 399 24 696 766 532
Cuts at: 3411 4369 442
Size: 958 1753
Fragments arranged by size:
1753 958 766 696 532 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2327 1056
Size: 1271 4409
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2914 2914
Size: 5680
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4340 1239
Size: 3101 2579
Bpu10I CC'TnA_GC
Cuts at: 2507 2507
Size: 5680
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2552 3805 4670 4968 2552
Size: 1253 865 298 3264
Fragments arranged by size:
3264 1253 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2261 4343 882
Size: 617 762 2082 2219
Fragments arranged by size:
2219 2082 762 617
BsaAI yAC'GTr
Cuts at: 493 1204 2320 2602 2770 3471 493
Size: 711 1116 282 168 701 2702
Fragments arranged by size:
2702 1116 711 701 282 168
BsaBI GATnn'nnATC
Cuts at: 2730 5674 2730
Size: 2944 2736
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3487 3869 4504 275
Size: 53 83 186 936 1954 382 635 1451
Fragments arranged by size:
1954 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4113 4511 4785 4932 5343 4113
Size: 398 274 147 411 4450
Fragments arranged by size:
4450 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5455
Fragments arranged by size:
5455 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5680
BseYI C'CCAG_C
Cuts at: 2402 2783 4883 5379 2402
Size: 381 2100 496 2703
Fragments arranged by size:
2703 2100 496 381
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2198 2211 2405 1163
Size: 573 462 13 194 4438
Fragments arranged by size:
4438 573 462 194 13
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3130 3891 4919 1540
Size: 275 186 1129 761 1028 2301
Fragments arranged by size:
2301 1129 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2646 3741 3826 4897 2646
Size: 1095 85 1071 3429
Fragments arranged by size:
3429 1095 1071 85
BsmI GAATG_Cn'
Cuts at: 3191 3284 3191
Size: 93 5587
BsmBI CGTCTCn'nnnn_
Cuts at: 2526 2526
Size: 5680
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2313 2646 2999 3741 3826 4897 1391
Size: 399 523 333 353 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 523 399 353 333 85
BspEI T'CCGG_A
Cuts at: 5343 5343
Size: 5680
BspHI T'CATG_A
Cuts at: 3569 5299 3569
Size: 1730 3950
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2751 3035 844
Size: 1907 284 3489
Fragments arranged by size:
3489 1907 284
BsrBI CCG'CTC
Cuts at: 1394 1823 2295 3567 1394
Size: 429 472 1272 3507
Fragments arranged by size:
3507 1272 472 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2356 4175 4349 65
Size: 1183 1108 1819 174 1396
Fragments arranged by size:
1819 1396 1183 1108 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4324 4511 1700
Size: 110 2514 187 2869
Fragments arranged by size:
2869 2514 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5680
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5680
BssSI C'ACGA_G
Cuts at: 3740 4752 3740
Size: 1012 4668
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2743 794
Size: 1949 3731
BstBI TT'CG_AA
Cuts at: 4484 4484
Size: 5680
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5680
BstYI r'GATC_y
Cuts at: 1972 2181 2379 2430 3010 3377 3762 3779 5231
Size: 209 198 51 580 367 385 17 1452
Cuts at: 5231 5317 5329 5675 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 580 385 367 346 209 198
86 51 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2094 513
Size: 552 939 90 4099
Fragments arranged by size:
4099 939 552 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4941
Fragments arranged by size:
4941 683 56
BtsI GCAGTG_nn'
Cuts at: 2656 2681 2703 3196 3990 4010 2656
Size: 25 22 493 794 20 4326
Fragments arranged by size:
4326 794 493 25 22 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5680
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5645
DraI TTT'AAA
Cuts at: 2984 2984
Size: 5680
DraIII CAC_nnn'GTG
Cuts at: 2486 2873 2486
Size: 387 5293
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2446 4687 809
Size: 507 1130 2241 1802
Fragments arranged by size:
2241 1802 1130 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2148 2454 2586
Size: 54 1425 50 275 18 318 306 132
Cuts at: 2586 2614 2678 2688 2719 3127 4016 5645 8
Size: 28 64 10 31 408 889 1629 43
Fragments arranged by size:
1629 1425 889 408 318 306 275 132
64 54 50 43 31 28 18 10
EagI C'GGCC_G
Cuts at: 1537 1812 3127 1537
Size: 275 1315 4090
Fragments arranged by size:
4090 1315 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3610 1353
Size: 2257 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4267 4641 4787 387
Size: 1064 138 2678 374 146 1280
Fragments arranged by size:
2678 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2880 3743 4340 5127 5395 1239
Size: 393 1248 863 597 787 268 1524
Fragments arranged by size:
1524 1248 863 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2113 2526 2824 2113
Size: 413 298 4969
Fragments arranged by size:
4969 413 298
EcoRI G'AATT_C
Cuts at: 2989 3367 2989
Size: 378 5302
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2962 2962
Size: 5680
FspI TGC'GCA
Cuts at: 4186 4186
Size: 5680
HaeII r_GCGC'y
Cuts at: 1678 2024 2392 4827 1678
Size: 346 368 2435 2531
Fragments arranged by size:
2531 2435 368 346
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2270 2302 4321 4353 4395
Size: 32 135 32 698 32 2019 32 42
Cuts at: 4395 4427 1373
Size: 32 2626
Fragments arranged by size:
2626 2019 698 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3024 3270 669
Size: 988 281 1086 246 3079
Fragments arranged by size:
3079 1086 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5680
HpaI GTT'AAC
Cuts at: 3270 3270
Size: 5680
KpnI G_GTAC'C
Cuts at: 3005 3005
Size: 5680
MfeI C'AATT_G
Cuts at: 3279 3279
Size: 5680
MluI A'CGCG_T
Cuts at: 5556 5556
Size: 5680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4794 4978 1305
Size: 3489 184 2007
Fragments arranged by size:
3489 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2150 2456 2616 2690 10
Size: 54 1425 343 318 306 160 74 3000
Fragments arranged by size:
3000 1425 343 318 306 160 74 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2234 2462 3638 3997 4156 518
Size: 648 843 225 228 1176 359 159 2042
Fragments arranged by size:
2042 1176 843 648 359 228 225 159
MspA1I CmG'CkG
Cuts at: 1606 2557 2578 3096 3773 4921 5166 1606
Size: 951 21 518 677 1148 245 2120
Fragments arranged by size:
2120 1148 951 677 518 245 21
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4189
Fragments arranged by size:
4189 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5680
NheI G'CTAG_C
Cuts at: 1052 2323 1052
Size: 1271 4409
NotI GC'GGCC_GC
Cuts at: 3127 3127
Size: 5680
NspI r_CATG'y
Cuts at: 1132 1229 2014 3038 4583 1132
Size: 97 785 1024 1545 2229
Fragments arranged by size:
2229 1545 1024 785 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5680
PciI A'CATG_T
Cuts at: 2010 4579 2010
Size: 2569 3111
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2391 2662 1108
Size: 1283 271 4126
Fragments arranged by size:
4126 1283 271
PfoI T'CCnGG_A
Cuts at: 2262 2262
Size: 5680
PmeI GTTT'AAAC
Cuts at: 2984 2984
Size: 5680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3749 3781 5262 5294 3749
Size: 32 1481 32 4135
Fragments arranged by size:
4135 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2113 2824 2113
Size: 711 4969
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5680
PsiI TTA'TAA
Cuts at: 3250 3250
Size: 5680
PstI C_TGCA'G
Cuts at: 830 2739 3032 830
Size: 1909 293 3478
Fragments arranged by size:
3478 1909 293
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5680
PvuII CAG'CTG
Cuts at: 2557 2578 2557
Size: 21 5659
SalI G'TCGA_C
Cuts at: 1936 3022 1936
Size: 1086 4594
SanDI GG'GwC_CC
Cuts at: 2113 2113
Size: 5680
SbfI CC_TGCA'GG
Cuts at: 3032 3032
Size: 5680
ScaI AGT'ACT
Cuts at: 3928 5640 3928
Size: 1712 3968
SfcI C'TryA_G
Cuts at: 826 1045 2735 3028 4163 4844 5035 826
Size: 219 1690 293 1135 681 191 1471
Fragments arranged by size:
1690 1471 1135 681 293 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5680
SmaI CCC'GGG
Cuts at: 1872 2141 3007 1872
Size: 269 866 4545
Fragments arranged by size:
4545 866 269
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2567 3784 4685 4947 820
Size: 197 926 624 1217 901 262 1553
Fragments arranged by size:
1553 1217 926 901 624 262 197
SnaBI TAC'GTA
Cuts at: 493 3471 493
Size: 2978 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5680
SphI G_CATG'C
Cuts at: 1132 3038 1132
Size: 1906 3774
SspI AAT'ATT
Cuts at: 5 52 3604 5
Size: 47 3552 2081
Fragments arranged by size:
3552 2081 47
StuI AGG'CCT
Cuts at: 1517 2930 1517
Size: 1413 4267
StyI C'CwwG_G
Cuts at: 513 1065 2004 2157 2344 3119 513
Size: 552 939 153 187 775 3074
Fragments arranged by size:
3074 939 775 552 187 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2087 2177 2861 3707 3724 3877 4062
Size: 1255 507 90 684 846 17 153 185
Cuts at: 4062 4507 325
Size: 445 1498
Fragments arranged by size:
1498 1255 846 684 507 445 185 153
90 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3926 5638 96
Size: 274 80 33 51 1443 1949 1712 138
Fragments arranged by size:
1949 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5640 5672 5640
Size: 32 5648
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5680
XbaI T'CTAG_A
Cuts at: 3016 3016
Size: 5680
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2342 2449 2464 2342
Size: 107 15 5558
Fragments arranged by size:
5558 107 15
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5680
XmaI C'CCGG_G
Cuts at: 1870 2139 3005 1870
Size: 269 866 4545
Fragments arranged by size:
4545 866 269
XmnI GAAnn'nnTTC
Cuts at: 3809 3809
Size: 5680
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3488 276
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI
SexAI SfiI SfoI SrfI SwaI