Restriction map for
pFN21AE1805
(Circular) (Six-base) MAPSORT of: pFN21AE1805.seq Check: 7891 from: 1 to: 5665
pFN21AE1805 5665 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2078 2078
Size: 5665
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3475 278
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3008 1937
Size: 1071 4594
Acc65I G'GTAC_C
Cuts at: 2986 2986
Size: 5665
AclI AA'CG_TT
Cuts at: 1563 3792 4165 1563
Size: 2229 373 3063
Fragments arranged by size:
3063 2229 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2425 3728 5112 5380 1632
Size: 793 1303 1384 268 1917
Fragments arranged by size:
1917 1384 1303 793 268
AflII C'TTAA_G
Cuts at: 820 1017 2960 820
Size: 197 1943 3525
Fragments arranged by size:
3525 1943 197
AflIII A'CryG_T
Cuts at: 1203 4564 5541 1203
Size: 3361 977 1327
Fragments arranged by size:
3361 1327 977
AgeI A'CCGG_T
Cuts at: 4496 4496
Size: 5665
AhdI GACnn_n'nnGTC
Cuts at: 4394 4394
Size: 5665
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5665
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5665
AlwNI CAG_nnn'CTG
Cuts at: 3408 4980 3408
Size: 1572 4093
ApaLI G'TGCA_C
Cuts at: 2093 2459 3722 4878 2093
Size: 366 1263 1156 2880
Fragments arranged by size:
2880 1263 1156 366
ApoI r'AATT_y
Cuts at: 1493 2329 2597 2974 3200 3352 5461 1493
Size: 836 268 377 226 152 2109 1697
Fragments arranged by size:
2109 1697 836 377 268 226 152
AscI GG'CGCG_CC
Cuts at: 2043 2043
Size: 5665
AseI AT'TA_AT
Cuts at: 160 1032 4219 160
Size: 872 3187 1606
Fragments arranged by size:
3187 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
AvaI C'yCGr_G
Cuts at: 1870 1943 2990 1870
Size: 73 1047 4545
Fragments arranged by size:
4545 1047 73
BamHI G'GATC_C
Cuts at: 2995 3362 2995
Size: 367 5298
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2986 4441 618
Size: 325 217 627 1199 1455 1842
Fragments arranged by size:
1842 1455 1199 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2298 2804 2984 1391
Size: 907 506 180 4072
Fragments arranged by size:
4072 907 506 180
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2591 928
Size: 971 692 4002
Fragments arranged by size:
4002 971 692
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3856 3890 1903
Size: 34 50 34 1835 34 3678
Fragments arranged by size:
3678 1835 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3557 4767 3557
Size: 1210 4455
BclI T'GATC_A
Cuts at: 1548 2373 1548
Size: 825 4840
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4276 136
Size: 107 122 71 631 605 2604 1525
Fragments arranged by size:
2604 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5660 5660
Size: 5665
BlpI GC'TnA_GC
Cuts at: 3082 3082
Size: 5665
Bme1580I G_kGCm'C
Cuts at: 1790 2097 2463 2621 3726 4882 1790
Size: 307 366 158 1105 1156 2573
Fragments arranged by size:
2573 1156 1105 366 307 158
BmgBI CAC'GTC
Cuts at: 1318 2839 1318
Size: 1521 4144
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3396 4354 442
Size: 416 53 83 399 24 1979 958 1753
Fragments arranged by size:
1979 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5665
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2843 4325 1239
Size: 1604 1482 2579
Fragments arranged by size:
2579 1604 1482
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2189 2486 2936 3790 4655 4953 2189
Size: 297 450 854 865 298 2901
Fragments arranged by size:
2901 865 854 450 298 297
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4328 882
Size: 617 2829 2219
Fragments arranged by size:
2829 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2134 3456 493
Size: 711 930 1322 2702
Fragments arranged by size:
2702 1322 930 711
BsaBI GATnn'nnATC
Cuts at: 5659 5659
Size: 5665
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3472 3854 4489 275
Size: 53 83 186 936 1939 382 635 1451
Fragments arranged by size:
1939 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4098 4496 4770 4917 5328 4098
Size: 398 274 147 411 4435
Fragments arranged by size:
4435 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2375 2405 2453 2483 1178
Size: 30 165 30 972 30 48 30 4360
Fragments arranged by size:
4360 972 165 48 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2461 1186
Size: 1275 4390
BseYI C'CCAG_C
Cuts at: 2113 2551 2709 2813 4868 5364 2113
Size: 438 158 104 2055 496 2414
Fragments arranged by size:
2414 2055 496 438 158 104
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2156 2402 2921 1163
Size: 573 420 246 519 3907
Fragments arranged by size:
3907 573 519 420 246
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3115 3876 4904 1540
Size: 275 186 1114 761 1028 2301
Fragments arranged by size:
2301 1114 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2097 2439 2463 3726 3811 4882 2097
Size: 342 24 1263 85 1071 2880
Fragments arranged by size:
2880 1263 1071 342 85 24
BsmI GAATG_Cn'
Cuts at: 3176 3269 3176
Size: 93 5572
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2097 2298 2439 2463 2621 2804 2984
Size: 399 307 201 141 24 158 183 180
Cuts at: 2984 3726 3811 4882 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 307 201 183 180
158 141 85 24
BspEI T'CCGG_A
Cuts at: 5328 5328
Size: 5665
BspHI T'CATG_A
Cuts at: 3554 5284 3554
Size: 1730 3935
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2078 3020 844
Size: 1234 942 3489
Fragments arranged by size:
3489 1234 942
BsrBI CCG'CTC
Cuts at: 1394 1823 2775 3552 1394
Size: 429 952 777 3507
Fragments arranged by size:
3507 952 777 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2640 4160 4334 65
Size: 1183 1392 1520 174 1396
Fragments arranged by size:
1520 1396 1392 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2079 4309 4496 1700
Size: 110 269 2230 187 2869
Fragments arranged by size:
2869 2230 269 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5665
BssHII G'CGCG_C
Cuts at: 1925 2043 1925
Size: 118 5547
BssSI C'ACGA_G
Cuts at: 3725 4737 3725
Size: 1012 4653
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2425 794
Size: 1631 4034
BstBI TT'CG_AA
Cuts at: 4469 4469
Size: 5665
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5665
BstXI CCAn_nnnn'nTGG
Cuts at: 2320 2320
Size: 5665
BstYI r'GATC_y
Cuts at: 1972 2995 3362 3747 3764 5216 5302 5314 5660
Size: 1023 367 385 17 1452 86 12 346
Cuts at: 5660 1972
Size: 1977
Fragments arranged by size:
1977 1452 1023 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2454 513
Size: 552 1389 3724
Fragments arranged by size:
3724 1389 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4926
Fragments arranged by size:
4926 683 56
BtsI GCAGTG_nn'
Cuts at: 2884 3181 3975 3995 2884
Size: 297 794 20 4554
Fragments arranged by size:
4554 794 297 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5665
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5630
DraI TTT'AAA
Cuts at: 2193 2969 2193
Size: 776 4889
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4672 809
Size: 507 3356 1802
Fragments arranged by size:
3356 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2128 2668 3112
Size: 54 1425 50 275 18 298 540 444
Cuts at: 3112 4001 5630 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 540 444 298 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3112 1537
Size: 275 1300 4090
Fragments arranged by size:
4090 1300 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2136 2792 3595 1353
Size: 783 656 803 3423
Fragments arranged by size:
3423 803 783 656
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4252 4626 4772 387
Size: 1064 138 2663 374 146 1280
Fragments arranged by size:
2663 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2425 2843 3728 4325 5112 5380 1239
Size: 393 793 418 885 597 787 268 1524
Fragments arranged by size:
1524 885 793 787 597 418 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2446 2446
Size: 5665
EcoRI G'AATT_C
Cuts at: 2329 2974 3352 2329
Size: 645 378 4642
Fragments arranged by size:
4642 645 378
FspI TGC'GCA
Cuts at: 4171 4171
Size: 5665
HaeII r_GCGC'y
Cuts at: 1678 2120 2127 2360 4812 1678
Size: 442 7 233 2452 2531
Fragments arranged by size:
2531 2452 442 233 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2216 2248 4306 4338 4380
Size: 32 135 32 644 32 2058 32 42
Cuts at: 4380 4412 1373
Size: 32 2626
Fragments arranged by size:
2626 2058 644 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2689 3009 3255 669
Size: 988 281 751 320 246 3079
Fragments arranged by size:
3079 988 751 320 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5665
HpaI GTT'AAC
Cuts at: 3255 3255
Size: 5665
KpnI G_GTAC'C
Cuts at: 2990 2990
Size: 5665
MfeI C'AATT_G
Cuts at: 3264 3264
Size: 5665
MluI A'CGCG_T
Cuts at: 5541 5541
Size: 5665
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4779 4963 1305
Size: 3474 184 2007
Fragments arranged by size:
3474 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2130 2670 10
Size: 54 1425 343 298 540 3005
Fragments arranged by size:
3005 1425 540 343 298 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2072 3623 3982 4141 518
Size: 648 843 63 1551 359 159 2042
Fragments arranged by size:
2042 1551 843 648 359 159 63
MspA1I CmG'CkG
Cuts at: 1606 2551 2765 3081 3758 4906 5151 1606
Size: 945 214 316 677 1148 245 2120
Fragments arranged by size:
2120 1148 945 677 316 245 214
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5113
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5665
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5665
NotI GC'GGCC_GC
Cuts at: 3112 3112
Size: 5665
NspI r_CATG'y
Cuts at: 1132 1229 2077 3023 4568 1132
Size: 97 848 946 1545 2229
Fragments arranged by size:
2229 1545 946 848 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5665
PciI A'CATG_T
Cuts at: 4564 4564
Size: 5665
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5665
PmeI GTTT'AAAC
Cuts at: 2969 2969
Size: 5665
PmlI CAC'GTG
Cuts at: 2134 2134
Size: 5665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3734 3766 5247 5279 3734
Size: 32 1481 32 4120
Fragments arranged by size:
4120 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2446 2446
Size: 5665
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5665
PsiI TTA'TAA
Cuts at: 3235 3235
Size: 5665
PstI C_TGCA'G
Cuts at: 830 2221 2879 3017 830
Size: 1391 658 138 3478
Fragments arranged by size:
3478 1391 658 138
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5665
PvuII CAG'CTG
Cuts at: 2551 2551
Size: 5665
SalI G'TCGA_C
Cuts at: 1936 3007 1936
Size: 1071 4594
SapI GCTCTTCn'nnn_
Cuts at: 2792 2792
Size: 5665
SbfI CC_TGCA'GG
Cuts at: 3017 3017
Size: 5665
ScaI AGT'ACT
Cuts at: 3913 5625 3913
Size: 1712 3953
SexAI A'CCwGG_T
Cuts at: 2016 2016
Size: 5665
SfcI C'TryA_G
Cuts at: 826 1045 2217 2875 3013 4148 4829 5020 826
Size: 219 1172 658 138 1135 681 191 1471
Fragments arranged by size:
1471 1172 1135 681 658 219 191 138
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5665
SmaI CCC'GGG
Cuts at: 1872 2992 1872
Size: 1120 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2168 2501 2951 2960 3769 4670
Size: 197 926 225 333 450 9 809 901
Cuts at: 4670 4932 820
Size: 262 1553
Fragments arranged by size:
1553 926 901 809 450 333 262 225
197 9
SnaBI TAC'GTA
Cuts at: 493 3456 493
Size: 2963 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5665
SphI G_CATG'C
Cuts at: 1132 3023 1132
Size: 1891 3774
SspI AAT'ATT
Cuts at: 5 52 3589 5
Size: 47 3537 2081
Fragments arranged by size:
3537 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5665
StyI C'CwwG_G
Cuts at: 513 1065 2804 3104 513
Size: 552 1739 300 3074
Fragments arranged by size:
3074 1739 552 300
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2464 3692 3709 3862 4047 4492 325
Size: 1255 884 1228 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1228 884 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2854 3911 5623
Size: 274 80 33 51 1443 877 1057 1712
Cuts at: 5623 96
Size: 138
Fragments arranged by size:
1712 1443 1057 877 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2453 2485 5625 5657 2453
Size: 32 3140 32 2461
Fragments arranged by size:
3140 2461 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5665
XbaI T'CTAG_A
Cuts at: 3001 3001
Size: 5665
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2317 2317
Size: 5665
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5665
XmaI C'CCGG_G
Cuts at: 1870 2990 1870
Size: 1120 4545
XmnI GAAnn'nnTTC
Cuts at: 2496 3794 2496
Size: 1298 4367
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3473 276
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI