Restriction map for
pFN21AE1804
(Circular) (Six-base) MAPSORT of: pFN21AE1804.seq Check: 5402 from: 1 to: 5659
pFN21AE1804 5659 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3469 278
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2437 3002 1937
Size: 500 565 4594
Fragments arranged by size:
4594 565 500
Acc65I G'GTAC_C
Cuts at: 2980 2980
Size: 5659
AclI AA'CG_TT
Cuts at: 1563 3786 4159 1563
Size: 2223 373 3063
Fragments arranged by size:
3063 2223 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2774 3722 5106 5374 1632
Size: 1142 948 1384 268 1917
Fragments arranged by size:
1917 1384 1142 948 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5462
AflIII A'CryG_T
Cuts at: 1203 4558 5535 1203
Size: 3355 977 1327
Fragments arranged by size:
3355 1327 977
AgeI A'CCGG_T
Cuts at: 4490 4490
Size: 5659
AhdI GACnn_n'nnGTC
Cuts at: 4388 4388
Size: 5659
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5659
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5659
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2750 2782 2750
Size: 32 5627
AlwNI CAG_nnn'CTG
Cuts at: 2047 2059 3402 4974 2047
Size: 12 1343 1572 2732
Fragments arranged by size:
2732 1572 1343 12
ApaLI G'TGCA_C
Cuts at: 3716 4872 3716
Size: 1156 4503
ApoI r'AATT_y
Cuts at: 1493 2634 2968 3194 3346 5455 1493
Size: 1141 334 226 152 2109 1697
Fragments arranged by size:
2109 1697 1141 334 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2784 4213 160
Size: 872 1752 1429 1606
Fragments arranged by size:
1752 1606 1429 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
AvaI C'yCGr_G
Cuts at: 1870 1943 2183 2984 1870
Size: 73 240 801 4545
Fragments arranged by size:
4545 801 240 73
BamHI G'GATC_C
Cuts at: 2245 2989 3356 2245
Size: 744 367 4548
Fragments arranged by size:
4548 744 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2117 2980 4435 618
Size: 325 217 627 330 863 1455 1842
Fragments arranged by size:
1842 1455 863 627 330 325 217
BanII G_rGCy'C
Cuts at: 1391 2978 1391
Size: 1587 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2493 928
Size: 971 594 4094
Fragments arranged by size:
4094 971 594
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3850 3884 1903
Size: 34 1913 34 3678
Fragments arranged by size:
3678 1913 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3551 4761 3551
Size: 1210 4449
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5659
BfrBI ATG'CAT
Cuts at: 2686 2686
Size: 5659
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4270 136
Size: 107 122 71 631 605 337 2261 1525
Fragments arranged by size:
2261 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2673 5654 2673
Size: 2981 2678
BlpI GC'TnA_GC
Cuts at: 3076 3076
Size: 5659
Bme1580I G_kGCm'C
Cuts at: 1790 2120 3720 4876 1790
Size: 330 1600 1156 2573
Fragments arranged by size:
2573 1600 1156 330
BmgBI CAC'GTC
Cuts at: 1318 2569 1318
Size: 1251 4408
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3390 4348 442
Size: 416 53 83 399 24 1973 958 1753
Fragments arranged by size:
1973 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5659
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2024 2630 4319 1239
Size: 785 606 1689 2579
Fragments arranged by size:
2579 1689 785 606
Bpu10I CC'TnA_GC
Cuts at: 2390 2390
Size: 5659
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2446 3784 4649 4947 2446
Size: 1338 865 298 3158
Fragments arranged by size:
3158 1338 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4322 882
Size: 617 2823 2219
Fragments arranged by size:
2823 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3450 493
Size: 711 2246 2702
Fragments arranged by size:
2702 2246 711
BsaBI GATnn'nnATC
Cuts at: 5653 5653
Size: 5659
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3466 3848 4483 275
Size: 53 83 186 936 1933 382 635 1451
Fragments arranged by size:
1933 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4092 4490 4764 4911 5322 4092
Size: 398 274 147 411 4429
Fragments arranged by size:
4429 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5434
Fragments arranged by size:
5434 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5659
BseYI C'CCAG_C
Cuts at: 4862 5358 4862
Size: 496 5163
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2188 1163
Size: 573 452 4634
Fragments arranged by size:
4634 573 452
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3109 3870 4898 1540
Size: 275 186 1108 761 1028 2301
Fragments arranged by size:
2301 1108 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3720 3805 4876 3720
Size: 85 1071 4503
Fragments arranged by size:
4503 1071 85
BsmI GAATG_Cn'
Cuts at: 3170 3263 3170
Size: 93 5566
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2120 2978 3720 3805 4876 1391
Size: 399 330 858 742 85 1071 2174
Fragments arranged by size:
2174 1071 858 742 399 330 85
BspEI T'CCGG_A
Cuts at: 5322 5322
Size: 5659
BspHI T'CATG_A
Cuts at: 3548 5278 3548
Size: 1730 3929
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3014 844
Size: 2170 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3546 1394
Size: 429 1723 3507
Fragments arranged by size:
3507 1723 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4154 4328 65
Size: 1183 2906 174 1396
Fragments arranged by size:
2906 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4303 4490 1700
Size: 110 2493 187 2869
Fragments arranged by size:
2869 2493 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5659
BssHII G'CGCG_C
Cuts at: 1925 2093 2095 2097 1925
Size: 168 2 2 5487
Fragments arranged by size:
5487 168 2 2
BssSI C'ACGA_G
Cuts at: 3719 4731 3719
Size: 1012 4647
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2059 794
Size: 1265 4394
BstBI TT'CG_AA
Cuts at: 4463 4463
Size: 5659
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5659
BstYI r'GATC_y
Cuts at: 1972 2245 2673 2989 3356 3741 3758 5210 5296
Size: 273 428 316 367 385 17 1452 86
Cuts at: 5296 5308 5654 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 428 385 367 346 316 273
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4168
Fragments arranged by size:
4168 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2013 2409 487
Size: 683 56 787 396 3737
Fragments arranged by size:
3737 787 683 396 56
BtsI GCAGTG_nn'
Cuts at: 2171 3175 3969 3989 2171
Size: 1004 794 20 3841
Fragments arranged by size:
3841 1004 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5659
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5624
DraI TTT'AAA
Cuts at: 2227 2963 2227
Size: 736 4923
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4666 809
Size: 507 3350 1802
Fragments arranged by size:
3350 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3106 3995 5624
Size: 54 1425 50 275 18 1276 889 1629
Cuts at: 5624 8
Size: 43
Fragments arranged by size:
1629 1425 1276 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3106 1537
Size: 275 1294 4090
Fragments arranged by size:
4090 1294 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3589 1353
Size: 2236 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4246 4620 4766 387
Size: 1064 138 2657 374 146 1280
Fragments arranged by size:
2657 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2024 2630 2774 3722 4319 5106 5374
Size: 393 392 606 144 948 597 787 268
Cuts at: 5374 1239
Size: 1524
Fragments arranged by size:
1524 948 787 606 597 393 392 268
144
EcoNI CCTnn'n_nnAGG
Cuts at: 2648 2648
Size: 5659
EcoO109I rG'GnC_Cy
Cuts at: 2699 2699
Size: 5659
EcoRI G'AATT_C
Cuts at: 2968 3346 2968
Size: 378 5281
FspI TGC'GCA
Cuts at: 4165 4165
Size: 5659
HaeII r_GCGC'y
Cuts at: 1678 2237 4806 1678
Size: 559 2569 2531
Fragments arranged by size:
2569 2531 559
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2237 2269 2419 2451 2453
Size: 32 135 32 665 32 150 32 2
Cuts at: 2453 2485 4300 4332 4374 4406 1373
Size: 32 1815 32 42 32 2626
Fragments arranged by size:
2626 1815 665 150 135 42 32 32
32 32 32 32 32 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2353 2438 3003 3249 669
Size: 988 281 415 85 565 246 3079
Fragments arranged by size:
3079 988 565 415 281 246 85
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5659
HpaI GTT'AAC
Cuts at: 2353 3249 2353
Size: 896 4763
KpnI G_GTAC'C
Cuts at: 2984 2984
Size: 5659
MfeI C'AATT_G
Cuts at: 3258 3258
Size: 5659
MluI A'CGCG_T
Cuts at: 5535 5535
Size: 5659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4773 4957 1305
Size: 3468 184 2007
Fragments arranged by size:
3468 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3837
Fragments arranged by size:
3837 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3617 3976 4135 518
Size: 648 2451 359 159 2042
Fragments arranged by size:
2451 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2044 2154 2191 2778 3075 3752 4900 5145
Size: 438 110 37 587 297 677 1148 245
Cuts at: 5145 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 587 438 297 245 110
37
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4168
Fragments arranged by size:
4168 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5659
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5659
NotI GC'GGCC_GC
Cuts at: 3106 3106
Size: 5659
NsiI A_TGCA'T
Cuts at: 2688 2688
Size: 5659
NspI r_CATG'y
Cuts at: 1132 1229 3017 4562 1132
Size: 97 1788 1545 2229
Fragments arranged by size:
2229 1788 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2014 1348
Size: 666 4993
PciI A'CATG_T
Cuts at: 4558 4558
Size: 5659
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5659
PfoI T'CCnGG_A
Cuts at: 2739 2945 2739
Size: 206 5453
PmeI GTTT'AAAC
Cuts at: 2963 2963
Size: 5659
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3728 3760 5241 5273 3728
Size: 32 1481 32 4114
Fragments arranged by size:
4114 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2699 2699
Size: 5659
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5659
PsiI TTA'TAA
Cuts at: 3229 3229
Size: 5659
PstI C_TGCA'G
Cuts at: 830 2055 3011 830
Size: 1225 956 3478
Fragments arranged by size:
3478 1225 956
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5659
PvuII CAG'CTG
Cuts at: 2044 2778 2044
Size: 734 4925
SalI G'TCGA_C
Cuts at: 1936 2436 3001 1936
Size: 500 565 4594
Fragments arranged by size:
4594 565 500
SanDI GG'GwC_CC
Cuts at: 2699 2699
Size: 5659
SbfI CC_TGCA'GG
Cuts at: 3011 3011
Size: 5659
ScaI AGT'ACT
Cuts at: 3907 5619 3907
Size: 1712 3947
SfcI C'TryA_G
Cuts at: 826 1045 2051 3007 4142 4823 5014 826
Size: 219 1006 956 1135 681 191 1471
Fragments arranged by size:
1471 1135 1006 956 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5659
SmaI CCC'GGG
Cuts at: 1872 2986 1872
Size: 1114 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2425 3763 4664 4926 820
Size: 197 926 482 1338 901 262 1553
Fragments arranged by size:
1553 1338 926 901 482 262 197
SnaBI TAC'GTA
Cuts at: 493 3450 493
Size: 2957 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5659
SphI G_CATG'C
Cuts at: 1132 3017 1132
Size: 1885 3774
SspI AAT'ATT
Cuts at: 5 52 3583 5
Size: 47 3531 2081
Fragments arranged by size:
3531 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5659
StyI C'CwwG_G
Cuts at: 513 1065 2004 2544 3098 513
Size: 552 939 540 554 3074
Fragments arranged by size:
3074 939 554 552 540
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2846 3686 3703 3856 4041 4486 325
Size: 1255 1266 840 17 153 185 445 1498
Fragments arranged by size:
1498 1266 1255 840 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2772 3905 5617
Size: 274 80 33 51 1443 795 1133 1712
Cuts at: 5617 96
Size: 138
Fragments arranged by size:
1712 1443 1133 795 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2691 2723 5619 5651 2691
Size: 32 2896 32 2699
Fragments arranged by size:
2896 2699 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5659
XbaI T'CTAG_A
Cuts at: 2995 2995
Size: 5659
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2953 2953
Size: 5659
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5659
XmaI C'CCGG_G
Cuts at: 1870 2984 1870
Size: 1114 4545
XmnI GAAnn'nnTTC
Cuts at: 3788 3788
Size: 5659
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3467 276
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SapI SexAI SfiI SfoI SrfI SwaI