Restriction map for
pFN21AE1798
(Circular) (Six-base) MAPSORT of: pFN21AE1798.seq Check: 3213 from: 1 to: 7111
pFN21AE1798 7111 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4921 278
Size: 53 83 186 936 3385 2468
Fragments arranged by size:
3385 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4454 1937
Size: 2517 4594
Acc65I G'GTAC_C
Cuts at: 4432 4432
Size: 7111
AclI AA'CG_TT
Cuts at: 1563 5238 5611 1563
Size: 3675 373 3063
Fragments arranged by size:
3675 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2180 3170 3535 3761 4184 5174 6558 6826
Size: 548 990 365 226 423 990 1384 268
Cuts at: 6826 1632
Size: 1917
Fragments arranged by size:
1917 1384 990 990 548 423 365 268
226
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6914
AflIII A'CryG_T
Cuts at: 1203 3483 3893 6010 6987 1203
Size: 2280 410 2117 977 1327
Fragments arranged by size:
2280 2117 1327 977 410
AgeI A'CCGG_T
Cuts at: 5942 5942
Size: 7111
AhdI GACnn_n'nnGTC
Cuts at: 5840 5840
Size: 7111
AleI CACnn'nnGTG
Cuts at: 1166 3552 1166
Size: 2386 4725
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7111
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2298 2330 2298
Size: 32 7079
AlwNI CAG_nnn'CTG
Cuts at: 3739 4409 4854 6426 3739
Size: 670 445 1572 4424
Fragments arranged by size:
4424 1572 670 445
ApaLI G'TGCA_C
Cuts at: 3626 5168 6324 3626
Size: 1542 1156 4413
Fragments arranged by size:
4413 1542 1156
ApoI r'AATT_y
Cuts at: 1493 2146 2788 3649 4420 4646 4798 6907 1493
Size: 653 642 861 771 226 152 2109 1697
Fragments arranged by size:
2109 1697 861 771 653 642 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5665 160
Size: 872 4633 1606
Fragments arranged by size:
4633 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7111
AvaI C'yCGr_G
Cuts at: 1870 1943 4436 1870
Size: 73 2493 4545
Fragments arranged by size:
4545 2493 73
AvrII C'CTAG_G
Cuts at: 2167 2167
Size: 7111
BamHI G'GATC_C
Cuts at: 3070 4441 4808 3070
Size: 1371 367 5373
Fragments arranged by size:
5373 1371 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2033 2665 4432 5887 618
Size: 325 217 627 246 632 1767 1455 1842
Fragments arranged by size:
1842 1767 1455 632 627 325 246 217
BanII G_rGCy'C
Cuts at: 1391 2157 3783 4430 1391
Size: 766 1626 647 4072
Fragments arranged by size:
4072 1626 766 647
BbeI G_GCGC'C
Cuts at: 2037 2037
Size: 7111
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3749 928
Size: 971 1850 4290
Fragments arranged by size:
4290 1850 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5302 5336 1903
Size: 34 3365 34 3678
Fragments arranged by size:
3678 3365 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3982 5003 6213 3982
Size: 1021 1210 4880
Fragments arranged by size:
4880 1210 1021
BclI T'GATC_A
Cuts at: 1548 3472 1548
Size: 1924 5187
BfrBI ATG'CAT
Cuts at: 3610 3610
Size: 7111
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4223 5722 136
Size: 107 122 71 631 605 2551 1499 1525
Fragments arranged by size:
2551 1525 1499 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7106 7106
Size: 7111
BlpI GC'TnA_GC
Cuts at: 4528 4528
Size: 7111
Bme1580I G_kGCm'C
Cuts at: 1790 3630 5172 6328 1790
Size: 1840 1542 1156 2573
Fragments arranged by size:
2573 1840 1542 1156
BmgBI CAC'GTC
Cuts at: 1318 3894 1318
Size: 2576 4535
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4842 5800 442
Size: 416 53 83 399 24 3425 958 1753
Fragments arranged by size:
3425 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7111
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2093 2093
Size: 7111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2196 2775 3234 5771 1239
Size: 957 579 459 2537 2579
Fragments arranged by size:
2579 2537 957 579 459
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2564 4066 5236 6101 6399 2564
Size: 1502 1170 865 298 3276
Fragments arranged by size:
3276 1502 1170 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4373 5774 882
Size: 617 2874 1401 2219
Fragments arranged by size:
2874 2219 1401 617
BsaAI yAC'GTr
Cuts at: 493 1204 2924 4902 493
Size: 711 1720 1978 2702
Fragments arranged by size:
2702 1978 1720 711
BsaBI GATnn'nnATC
Cuts at: 7105 7105
Size: 7111
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2034 3831 4918 5300
Size: 53 83 186 936 501 1797 1087 382
Cuts at: 5300 5935 275
Size: 635 1451
Fragments arranged by size:
1797 1451 1087 936 635 501 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3724 4105 4124 5544 5942 6216 6363 6774 3724
Size: 381 19 1420 398 274 147 411 4061
Fragments arranged by size:
4061 1420 411 398 381 274 147 19
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2472 2502 2713 2743 3067
Size: 30 165 30 1069 30 211 30 324
Cuts at: 3067 3097 4119 4149 1178
Size: 30 1022 30 4140
Fragments arranged by size:
4140 1069 1022 324 211 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2027 2069 2082 3528 1186
Size: 838 3 42 13 1446 4769
Fragments arranged by size:
4769 1446 838 42 13 3
BseYI C'CCAG_C
Cuts at: 4349 6314 6810 4349
Size: 1965 496 4650
Fragments arranged by size:
4650 1965 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2486 2985 1163
Size: 573 750 499 5289
Fragments arranged by size:
5289 750 573 499
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4561 5322 6350 1540
Size: 275 186 2560 761 1028 2301
Fragments arranged by size:
2560 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2157 3630 3783 5172 5257 6328 2157
Size: 1473 153 1389 85 1071 2940
Fragments arranged by size:
2940 1473 1389 1071 153 85
BsmI GAATG_Cn'
Cuts at: 4622 4715 4622
Size: 93 7018
BsmBI CGTCTCn'nnnn_
Cuts at: 3551 4259 3551
Size: 708 6403
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2157 3630 3783 4430 5172 5257 6328
Size: 399 367 1473 153 647 742 85 1071
Cuts at: 6328 1391
Size: 2174
Fragments arranged by size:
2174 1473 1071 742 647 399 367 153
85
BspEI T'CCGG_A
Cuts at: 4105 4124 6774 4105
Size: 19 2650 4442
Fragments arranged by size:
4442 2650 19
BspHI T'CATG_A
Cuts at: 3469 3576 3877 3915 5000 6730 3469
Size: 107 301 38 1085 1730 3850
Fragments arranged by size:
3850 1730 1085 301 107 38
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4466 844
Size: 3622 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4998 1394
Size: 429 3175 3507
Fragments arranged by size:
3507 3175 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3246 5606 5780 65
Size: 1183 1998 2360 174 1396
Fragments arranged by size:
2360 1998 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5755 5942 1700
Size: 110 3945 187 2869
Fragments arranged by size:
3945 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3840 96
Size: 3744 3367
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7111
BssSI C'ACGA_G
Cuts at: 2093 5171 6183 2093
Size: 3078 1012 3021
Fragments arranged by size:
3078 3021 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2098 2464 794
Size: 1304 366 5441
Fragments arranged by size:
5441 1304 366
BstBI TT'CG_AA
Cuts at: 5915 5915
Size: 7111
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7111
BstXI CCAn_nnnn'nTGG
Cuts at: 2225 3907 2225
Size: 1682 5429
BstYI r'GATC_y
Cuts at: 1972 2893 3070 3634 4015 4118 4441 4808 5193
Size: 921 177 564 381 103 323 367 385
Cuts at: 5193 5210 6662 6748 6760 7106 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 921 564 385 381 367 346
323 177 103 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2037 513
Size: 552 939 33 5587
Fragments arranged by size:
5587 939 552 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6372
Fragments arranged by size:
6372 683 56
BtsI GCAGTG_nn'
Cuts at: 4340 4627 5421 5441 4340
Size: 287 794 20 6010
Fragments arranged by size:
6010 794 287 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7111
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7076
DraI TTT'AAA
Cuts at: 4415 4415
Size: 7111
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6118 809
Size: 507 4802 1802
Fragments arranged by size:
4802 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2215 2569 4558
Size: 54 1425 50 275 18 385 354 1989
Cuts at: 4558 5447 7076 8
Size: 889 1629 43
Fragments arranged by size:
1989 1629 1425 889 385 354 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4558 1537
Size: 275 2746 4090
Fragments arranged by size:
4090 2746 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2897 2957 3048 3189 4391 5041 1353
Size: 1544 60 91 141 1202 650 3423
Fragments arranged by size:
3423 1544 1202 650 141 91 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5698 6072 6218 387
Size: 1064 138 4109 374 146 1280
Fragments arranged by size:
4109 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2155 3781 2155
Size: 1626 5485
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2180 2196 2775 3170 3234 3535 3761
Size: 393 548 16 579 395 64 301 226
Cuts at: 3761 4184 5174 5771 6558 6826 1239
Size: 423 990 597 787 268 1524
Fragments arranged by size:
1524 990 787 597 579 548 423 395
393 301 268 226 64 16
EcoO109I rG'GnC_Cy
Cuts at: 2164 4216 2164
Size: 2052 5059
EcoRI G'AATT_C
Cuts at: 4420 4798 4420
Size: 378 6733
EcoRV GAT'ATC
Cuts at: 2805 2805
Size: 7111
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3565 3565
Size: 7111
FspI TGC'GCA
Cuts at: 5617 5617
Size: 7111
HaeII r_GCGC'y
Cuts at: 1678 2037 6258 1678
Size: 359 4221 2531
Fragments arranged by size:
4221 2531 359
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2512 2544 2878 2910 5752
Size: 32 135 32 940 32 334 32 2842
Cuts at: 5752 5784 5826 5858 1373
Size: 32 42 32 2626
Fragments arranged by size:
2842 2626 940 334 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2713 4455 4701 669
Size: 988 281 775 1742 246 3079
Fragments arranged by size:
3079 1742 988 775 281 246
HindIII A'AGCT_T
Cuts at: 748 2540 3354 3584 748
Size: 1792 814 230 4275
Fragments arranged by size:
4275 1792 814 230
HpaI GTT'AAC
Cuts at: 4701 4701
Size: 7111
KasI G'GCGC_C
Cuts at: 2033 2033
Size: 7111
KpnI G_GTAC'C
Cuts at: 4436 4436
Size: 7111
MfeI C'AATT_G
Cuts at: 4710 4710
Size: 7111
MluI A'CGCG_T
Cuts at: 6987 6987
Size: 7111
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2458 3598 6225 6409 1305
Size: 1153 1140 2627 184 2007
Fragments arranged by size:
2627 2007 1153 1140 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2217 2571 10
Size: 54 1425 343 385 354 4550
Fragments arranged by size:
4550 1425 385 354 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3065 3552 3609 4143 5069 5428 5587
Size: 648 1899 487 57 534 926 359 159
Cuts at: 5587 518
Size: 2042
Fragments arranged by size:
2042 1899 926 648 534 487 359 159
57
MspA1I CmG'CkG
Cuts at: 1606 2028 2039 3170 3596 4081 4527 5204 6352
Size: 422 11 1131 426 485 446 677 1148
Cuts at: 6352 6597 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1131 677 485 446 426 422
245 11
NarI GG'CG_CC
Cuts at: 2034 2034
Size: 7111
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5620
Fragments arranged by size:
5620 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7111
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7111
NotI GC'GGCC_GC
Cuts at: 4558 4558
Size: 7111
NsiI A_TGCA'T
Cuts at: 3612 3612
Size: 7111
NspI r_CATG'y
Cuts at: 1132 1229 2126 3610 4469 6014 1132
Size: 97 897 1484 859 1545 2229
Fragments arranged by size:
2229 1545 1484 897 859 97
PasI CC'CwG_GG
Cuts at: 1348 3298 1348
Size: 1950 5161
PciI A'CATG_T
Cuts at: 6010 6010
Size: 7111
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2515 1108
Size: 1407 5704
PfoI T'CCnGG_A
Cuts at: 2316 3211 2316
Size: 895 6216
PmeI GTTT'AAAC
Cuts at: 4415 4415
Size: 7111
PmlI CAC'GTG
Cuts at: 2924 2924
Size: 7111
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5180 5212 6693 6725 5180
Size: 32 1481 32 5566
Fragments arranged by size:
5566 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2164 2164
Size: 7111
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7111
PsiI TTA'TAA
Cuts at: 4681 4681
Size: 7111
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2184 2216 2184
Size: 32 7079
PstI C_TGCA'G
Cuts at: 830 4347 4463 830
Size: 3517 116 3478
Fragments arranged by size:
3517 3478 116
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7111
PvuII CAG'CTG
Cuts at: 3170 3596 4081 3170
Size: 426 485 6200
Fragments arranged by size:
6200 485 426
SacI G_AGCT'C
Cuts at: 2157 3783 2157
Size: 1626 5485
SacII CC_GC'GG
Cuts at: 2040 2040
Size: 7111
SalI G'TCGA_C
Cuts at: 1936 4453 1936
Size: 2517 4594
SbfI CC_TGCA'GG
Cuts at: 4463 4463
Size: 7111
ScaI AGT'ACT
Cuts at: 5359 7071 5359
Size: 1712 5399
SfcI C'TryA_G
Cuts at: 826 1045 4343 4459 5594 6275 6466 826
Size: 219 3298 116 1135 681 191 1471
Fragments arranged by size:
3298 1471 1135 681 219 191 116
SfoI GGC'GCC
Cuts at: 2035 2035
Size: 7111
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7111
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7111
SmaI CCC'GGG
Cuts at: 1872 4438 1872
Size: 2566 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2543 4045 5215 6116 6378 820
Size: 197 926 600 1502 1170 901 262 1553
Fragments arranged by size:
1553 1502 1170 926 901 600 262 197
SnaBI TAC'GTA
Cuts at: 493 4902 493
Size: 4409 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7111
SphI G_CATG'C
Cuts at: 1132 4469 1132
Size: 3337 3774
SspI AAT'ATT
Cuts at: 5 52 2551 2817 3445 5035 5
Size: 47 2499 266 628 1590 2081
Fragments arranged by size:
2499 2081 1590 628 266 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7111
StyI C'CwwG_G
Cuts at: 513 1065 2004 2167 3997 4550 513
Size: 552 939 163 1830 553 3074
Fragments arranged by size:
3074 1830 939 553 552 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2717 3897 4345 5138 5155 5308 5493
Size: 1255 1137 1180 448 793 17 153 185
Cuts at: 5493 5938 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1180 1137 793 448 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3840 5357 7069
Size: 274 80 33 51 1443 1863 1517 1712
Cuts at: 7069 96
Size: 138
Fragments arranged by size:
1863 1712 1517 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2472 2504 2713 2745 3067 3099 4000 4032 4119
Size: 32 209 32 322 32 901 32 87
Cuts at: 4119 4151 7071 7103 2472
Size: 32 2920 32 2480
Fragments arranged by size:
2920 2480 901 322 209 87 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7111
XbaI T'CTAG_A
Cuts at: 4447 4447
Size: 7111
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2905 2905
Size: 7111
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7111
XmaI C'CCGG_G
Cuts at: 1870 4436 1870
Size: 2566 4545
XmnI GAAnn'nnTTC
Cuts at: 3465 3548 5240 3465
Size: 83 1692 5336
Fragments arranged by size:
5336 1692 83
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4919 276
Size: 53 83 186 936 3385 2468
Fragments arranged by size:
3385 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbvCI Bpu10I BsiWI
BstZ17I Bsu36I DraIII EcoNI FseI FspAI NaeI NgoMIV
NruI PacI PspOMI PspXI RsrII SanDI SapI SexAI
SfiI SrfI SwaI