Restriction map for
pFN21AE1797
(Circular) (Six-base) MAPSORT of: pFN21AE1797.seq Check: 5328 from: 1 to: 7234
pFN21AE1797 7234 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4256 4256
Size: 7234
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5044 278
Size: 53 83 186 936 3508 2468
Fragments arranged by size:
3508 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4577 1937
Size: 2640 4594
Acc65I G'GTAC_C
Cuts at: 4555 4555
Size: 7234
AclI AA'CG_TT
Cuts at: 1563 5361 5734 1563
Size: 3798 373 3063
Fragments arranged by size:
3798 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2390 2471 3167 4367 5297 6681 6949 1632
Size: 758 81 696 1200 930 1384 268 1917
Fragments arranged by size:
1917 1384 1200 930 758 696 268 81
AfeI AGC'GCT
Cuts at: 2014 2014
Size: 7234
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7037
AflIII A'CryG_T
Cuts at: 1203 6133 7110 1203
Size: 4930 977 1327
Fragments arranged by size:
4930 1327 977
AgeI A'CCGG_T
Cuts at: 6065 6065
Size: 7234
AhdI GACnn_n'nnGTC
Cuts at: 5963 5963
Size: 7234
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7234
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3752 1184
Size: 2568 4666
AlwNI CAG_nnn'CTG
Cuts at: 4977 6549 4977
Size: 1572 5662
ApaI G_GGCC'C
Cuts at: 3802 3820 3802
Size: 18 7216
ApaLI G'TGCA_C
Cuts at: 3620 5291 6447 3620
Size: 1671 1156 4407
Fragments arranged by size:
4407 1671 1156
ApoI r'AATT_y
Cuts at: 1493 2967 4326 4543 4769 4921 7030 1493
Size: 1474 1359 217 226 152 2109 1697
Fragments arranged by size:
2109 1697 1474 1359 226 217 152
AseI AT'TA_AT
Cuts at: 160 1032 5788 160
Size: 872 4756 1606
Fragments arranged by size:
4756 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7234
AvaI C'yCGr_G
Cuts at: 1870 1943 2648 3061 3130 3503 3831 4559 1870
Size: 73 705 413 69 373 328 728 4545
Fragments arranged by size:
4545 728 705 413 373 328 73 69
BamHI G'GATC_C
Cuts at: 4564 4931 4564
Size: 367 6867
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3253 3488 3981 4008 4555
Size: 325 217 627 1466 235 493 27 547
Cuts at: 4555 6010 618
Size: 1455 1842
Fragments arranged by size:
1842 1466 1455 627 547 493 325 235
217 27
BanII G_rGCy'C
Cuts at: 1391 3224 3231 3802 3820 4553 1391
Size: 1833 7 571 18 733 4072
Fragments arranged by size:
4072 1833 733 571 18 7
BbeI G_GCGC'C
Cuts at: 3257 3492 3257
Size: 235 6999
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3309 928
Size: 971 1410 4853
Fragments arranged by size:
4853 1410 971
BbvCI CC'TCA_GC
Cuts at: 4119 4119
Size: 7234
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4421 4455 5425 5459 1903
Size: 34 2484 34 970 34 3678
Fragments arranged by size:
3678 2484 970 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2314 2368 2807 5126 6336 2314
Size: 54 439 2319 1210 3212
Fragments arranged by size:
3212 2319 1210 439 54
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7234
BfrBI ATG'CAT
Cuts at: 3016 3016
Size: 7234
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2203 3639 5845
Size: 107 122 71 631 605 531 1436 2206
Cuts at: 5845 136
Size: 1525
Fragments arranged by size:
2206 1525 1436 631 605 531 122 107
71
BglII A'GATC_T
Cuts at: 4464 7229 4464
Size: 2765 4469
BlpI GC'TnA_GC
Cuts at: 3120 4089 4388 4651 3120
Size: 969 299 263 5703
Fragments arranged by size:
5703 969 299 263
Bme1580I G_kGCm'C
Cuts at: 1790 3624 3802 3820 3916 4011 4033 5295 6451
Size: 1834 178 18 96 95 22 1262 1156
Cuts at: 6451 1790
Size: 2573
Fragments arranged by size:
2573 1834 1262 1156 178 96 95 22
18
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7234
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2072 4965 5923
Size: 416 53 83 399 24 655 2893 958
Cuts at: 5923 442
Size: 1753
Fragments arranged by size:
2893 1753 958 655 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 4118 1056
Size: 3062 4172
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3145 3145
Size: 7234
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2321 2987 3173 3197 3896 3945 4115 4218
Size: 1082 666 186 24 699 49 170 103
Cuts at: 4218 4265 5894 1239
Size: 47 1629 2579
Fragments arranged by size:
2579 1629 1082 699 666 186 170 103
49 47 24
Bpu10I CC'TnA_GC
Cuts at: 4119 4119
Size: 7234
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3329 5359 6224 6522 3329
Size: 2030 865 298 4041
Fragments arranged by size:
4041 2030 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2093 5897 882
Size: 617 594 3804 2219
Fragments arranged by size:
3804 2219 617 594
BsaAI yAC'GTr
Cuts at: 493 1204 5025 493
Size: 711 3821 2702
Fragments arranged by size:
3821 2702 711
BsaBI GATnn'nnATC
Cuts at: 7228 7228
Size: 7234
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3254 3489 5041 5423
Size: 53 83 186 936 1721 235 1552 382
Cuts at: 5423 6058 275
Size: 635 1451
Fragments arranged by size:
1721 1552 1451 936 635 382 235 186
83 53
BsaWI w'CCGG_w
Cuts at: 2215 3688 4335 5667 6065 6339 6486 6897 2215
Size: 1473 647 1332 398 274 147 411 2552
Fragments arranged by size:
2552 1473 1332 647 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3257 3287 1178
Size: 30 165 30 1854 30 5125
Fragments arranged by size:
5125 1854 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2620 2714 2845 3194 3491 3605 4112 4475
Size: 1434 94 131 349 297 114 507 363
Cuts at: 4475 1186
Size: 3945
Fragments arranged by size:
3945 1434 507 363 349 297 131 114
94
BseYI C'CCAG_C
Cuts at: 2042 3701 3812 6437 6933 2042
Size: 1659 111 2625 496 2343
Fragments arranged by size:
2625 2343 1659 496 111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6661
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3477 4684 5445 6473 1540
Size: 275 186 1476 1207 761 1028 2301
Fragments arranged by size:
2301 1476 1207 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3231 3624 4488 5295 5380 6451 3231
Size: 393 864 807 85 1071 4014
Fragments arranged by size:
4014 1071 864 807 393 85
BsmI GAATG_Cn'
Cuts at: 4745 4838 4745
Size: 93 7141
BsmBI CGTCTCn'nnnn_
Cuts at: 3963 4472 3963
Size: 509 6725
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3224 3231 3624 3802 3820 3916 4011
Size: 399 1434 7 393 178 18 96 95
Cuts at: 4011 4033 4488 4553 5295 5380 6451 1391
Size: 22 455 65 742 85 1071 2174
Fragments arranged by size:
2174 1434 1071 742 455 399 393 178
96 95 85 65 22 18 7
BspEI T'CCGG_A
Cuts at: 2215 3688 6897 2215
Size: 1473 3209 2552
Fragments arranged by size:
3209 2552 1473
BspHI T'CATG_A
Cuts at: 5123 6853 5123
Size: 1730 5504
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4256 4589 844
Size: 3412 333 3489
Fragments arranged by size:
3489 3412 333
BsrBI CCG'CTC
Cuts at: 1394 1823 3399 3886 3954 4451 4525 5121 1394
Size: 429 1576 487 68 497 74 596 3507
Fragments arranged by size:
3507 1576 596 497 487 429 74 68
BsrDI GCAATG_nn'
Cuts at: 65 1248 2353 3754 5729 5903 65
Size: 1183 1105 1401 1975 174 1396
Fragments arranged by size:
1975 1401 1396 1183 1105 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3978 5878 6065 1700
Size: 110 2168 1900 187 2869
Fragments arranged by size:
2869 2168 1900 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7234
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7234
BssSI C'ACGA_G
Cuts at: 5294 6306 5294
Size: 1012 6222
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3619 3871 794
Size: 2825 252 4157
Fragments arranged by size:
4157 2825 252
BstBI TT'CG_AA
Cuts at: 6038 6038
Size: 7234
BstEII G'GTnAC_C
Cuts at: 1184 2137 1184
Size: 953 6281
BstXI CCAn_nnnn'nTGG
Cuts at: 4084 4294 4084
Size: 210 7024
BstYI r'GATC_y
Cuts at: 1972 2535 3195 4464 4564 4931 5316 5333 6785
Size: 563 660 1269 100 367 385 17 1452
Cuts at: 6785 6871 6883 7229 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1269 660 563 385 367 346
100 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2069 2742 2775 2793 3096 3471 513
Size: 552 1004 673 33 18 303 375 4276
Fragments arranged by size:
4276 1004 673 552 375 303 33 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3002 4070 4446 487
Size: 683 56 1776 1068 376 3275
Fragments arranged by size:
3275 1776 1068 683 376 56
BtsI GCAGTG_nn'
Cuts at: 2962 3028 3622 3872 4750 5544 5564 2962
Size: 66 594 250 878 794 20 4632
Fragments arranged by size:
4632 878 794 594 250 66 20
ClaI AT'CG_AT
Cuts at: 1554 4530 1554
Size: 2976 4258
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7199
DraI TTT'AAA
Cuts at: 4378 4538 4378
Size: 160 7074
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6241 809
Size: 507 4925 1802
Fragments arranged by size:
4925 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3037 3474 4155
Size: 54 1425 50 275 18 1207 437 681
Cuts at: 4155 4185 4681 5570 7199 8
Size: 30 496 889 1629 43
Fragments arranged by size:
1629 1425 1207 889 681 496 437 275
54 50 43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 3474 4681 1537
Size: 275 1662 1207 4090
Fragments arranged by size:
4090 1662 1207 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2164 2691 3204 5164 1353
Size: 811 527 513 1960 3423
Fragments arranged by size:
3423 1960 811 527 513
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2188 3617 3822 5821 6195 6341
Size: 1064 138 599 1429 205 1999 374 146
Cuts at: 6341 387
Size: 1280
Fragments arranged by size:
1999 1429 1280 1064 599 374 205 146
138
EcoICRI GAG'CTC
Cuts at: 3229 3229
Size: 7234
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2321 2390 2471 2987 3167 3173 3197
Size: 393 689 69 81 516 180 6 24
Cuts at: 3197 3896 3945 4115 4218 4265 4367 5297 5894
Size: 699 49 170 103 47 102 930 597
Cuts at: 5894 6681 6949 1239
Size: 787 268 1524
Fragments arranged by size:
1524 930 787 699 689 597 516 393
268 180 170 103 102 81 69 49
47 24 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2827 3080 4108 2827
Size: 253 1028 5953
Fragments arranged by size:
5953 1028 253
EcoO109I rG'GnC_Cy
Cuts at: 2820 3340 3798 3799 3816 3817 4048 2820
Size: 520 458 1 17 1 231 6006
Fragments arranged by size:
6006 520 458 231 17 1 1
EcoRI G'AATT_C
Cuts at: 2967 4543 4921 2967
Size: 1576 378 5280
Fragments arranged by size:
5280 1576 378
EcoRV GAT'ATC
Cuts at: 2910 2910
Size: 7234
FspI TGC'GCA
Cuts at: 5740 5740
Size: 7234
HaeII r_GCGC'y
Cuts at: 1678 2016 2895 3187 3257 3492 6381 1678
Size: 338 879 292 70 235 2889 2531
Fragments arranged by size:
2889 2531 879 338 292 235 70
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2469 2501 2525 2557 2821
Size: 32 135 32 897 32 24 32 264
Cuts at: 2821 2853 2924 2956 3317 3349 5875 5907 5949
Size: 32 71 32 361 32 2526 32 42
Cuts at: 5949 5981 1373
Size: 32 2626
Fragments arranged by size:
2626 2526 897 361 264 135 71 42
32 32 32 32 32 32 32 32
32 24
HincII GTy'rAC
Cuts at: 669 1657 1938 2806 4578 4824 669
Size: 988 281 868 1772 246 3079
Fragments arranged by size:
3079 1772 988 868 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7234
HpaI GTT'AAC
Cuts at: 4824 4824
Size: 7234
KasI G'GCGC_C
Cuts at: 3253 3488 3253
Size: 235 6999
KpnI G_GTAC'C
Cuts at: 4559 4559
Size: 7234
MfeI C'AATT_G
Cuts at: 4833 4833
Size: 7234
MluI A'CGCG_T
Cuts at: 7110 7110
Size: 7234
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2187 3138 6348 6532 1305
Size: 882 951 3210 184 2007
Fragments arranged by size:
3210 2007 951 882 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3039 4157 10
Size: 54 1425 343 1207 1118 3087
Fragments arranged by size:
3087 1425 1207 1118 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2942 3021 3567 5192 5551 5710 518
Size: 648 1776 79 546 1625 359 159 2042
Fragments arranged by size:
2042 1776 1625 648 546 359 159 79
MspA1I CmG'CkG
Cuts at: 1606 2071 2449 3119 3473 3616 4005 4093 4144
Size: 465 378 670 354 143 389 88 51
Cuts at: 4144 4650 5327 6475 6720 1606
Size: 506 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 670 506 465 389 378
354 245 143 88 51
NaeI GCC'GGC
Cuts at: 3980 3980
Size: 7234
NarI GG'CG_CC
Cuts at: 3254 3489 3254
Size: 235 6999
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6682
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7234
NgoMIV G'CCGG_C
Cuts at: 3978 3978
Size: 7234
NheI G'CTAG_C
Cuts at: 1052 4114 1052
Size: 3062 4172
NotI GC'GGCC_GC
Cuts at: 3474 4681 3474
Size: 1207 6027
NsiI A_TGCA'T
Cuts at: 3018 3018
Size: 7234
NspI r_CATG'y
Cuts at: 1132 1229 3545 4592 6137 1132
Size: 97 2316 1047 1545 2229
Fragments arranged by size:
2316 2229 1545 1047 97
PasI CC'CwG_GG
Cuts at: 1348 3635 4366 1348
Size: 2287 731 4216
Fragments arranged by size:
4216 2287 731
PciI A'CATG_T
Cuts at: 6133 6133
Size: 7234
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3175 1108
Size: 2067 5167
PfoI T'CCnGG_A
Cuts at: 2034 2298 2034
Size: 264 6970
PmeI GTTT'AAAC
Cuts at: 4538 4538
Size: 7234
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5303 5335 6816 6848 5303
Size: 32 1481 32 5689
Fragments arranged by size:
5689 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2820 3340 4048 2820
Size: 520 708 6006
Fragments arranged by size:
6006 708 520
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7234
PsiI TTA'TAA
Cuts at: 4804 4804
Size: 7234
PspOMI G'GGCC_C
Cuts at: 3798 3816 3798
Size: 18 7216
PstI C_TGCA'G
Cuts at: 830 3192 3825 4586 830
Size: 2362 633 761 3478
Fragments arranged by size:
3478 2362 761 633
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7234
PvuII CAG'CTG
Cuts at: 2449 3119 4093 2449
Size: 670 974 5590
Fragments arranged by size:
5590 974 670
SacI G_AGCT'C
Cuts at: 3231 3231
Size: 7234
SacII CC_GC'GG
Cuts at: 2072 3474 2072
Size: 1402 5832
SalI G'TCGA_C
Cuts at: 1936 4576 1936
Size: 2640 4594
SbfI CC_TGCA'GG
Cuts at: 4586 4586
Size: 7234
ScaI AGT'ACT
Cuts at: 5482 7194 5482
Size: 1712 5522
SfcI C'TryA_G
Cuts at: 826 1045 3188 3382 3821 4582 5717 6398 6589
Size: 219 2143 194 439 761 1135 681 191
Cuts at: 6589 826
Size: 1471
Fragments arranged by size:
2143 1471 1135 761 681 439 219 194
191
SfoI GGC'GCC
Cuts at: 3255 3490 3255
Size: 235 6999
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7234
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7234
SmaI CCC'GGG
Cuts at: 1872 3833 4561 1872
Size: 1961 728 4545
Fragments arranged by size:
4545 1961 728
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3130 3344 5338 6239 6501 820
Size: 197 926 1187 214 1994 901 262 1553
Fragments arranged by size:
1994 1553 1187 926 901 262 214 197
SnaBI TAC'GTA
Cuts at: 493 5025 493
Size: 4532 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7234
SphI G_CATG'C
Cuts at: 1132 4592 1132
Size: 3460 3774
SspI AAT'ATT
Cuts at: 5 52 5158 5
Size: 47 5106 2081
Fragments arranged by size:
5106 2081 47
StuI AGG'CCT
Cuts at: 1517 3932 4103 1517
Size: 2415 171 4648
Fragments arranged by size:
4648 2415 171
StyI C'CwwG_G
Cuts at: 513 1065 3081 3585 3681 3939 4673 513
Size: 552 2016 504 96 258 734 3074
Fragments arranged by size:
3074 2016 734 552 504 258 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2546 4058 4090 5261 5278 5431 5616
Size: 1255 966 1512 32 1171 17 153 185
Cuts at: 5616 6061 325
Size: 445 1498
Fragments arranged by size:
1512 1498 1255 1171 966 445 185 153
32 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5480 7192 96
Size: 274 80 33 51 1443 3503 1712 138
Fragments arranged by size:
3503 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3257 3289 7194 7226 3257
Size: 32 3905 32 3265
Fragments arranged by size:
3905 3265 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2242 1316
Size: 926 6308
XbaI T'CTAG_A
Cuts at: 4570 4570
Size: 7234
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4225 4225
Size: 7234
XhoI C'TCGA_G
Cuts at: 1943 3130 1943
Size: 1187 6047
XmaI C'CCGG_G
Cuts at: 1870 3831 4559 1870
Size: 1961 728 4545
Fragments arranged by size:
4545 1961 728
XmnI GAAnn'nnTTC
Cuts at: 4326 5363 4326
Size: 1037 6197
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5042 276
Size: 53 83 186 936 3508 2468
Fragments arranged by size:
3508 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BsiWI BstZ17I Bsu36I DraIII
FalI FseI FspAI NruI PacI PmlI PspXI PsrI
RsrII SanDI SapI SexAI SfiI SrfI SwaI