Restriction map for
pFN21AE1793
(Circular) (Six-base) MAPSORT of: pFN21AE1793.seq Check: 9109 from: 1 to: 5617
pFN21AE1793 5617 bp
With 182 enzymes: SgfI *
July 6, 2012 17:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3427 278
Size: 53 83 186 936 1891 2468
Fragments arranged by size:
2468 1891 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2960 1937
Size: 1023 4594
Acc65I G'GTAC_C
Cuts at: 2938 2938
Size: 5617
AclI AA'CG_TT
Cuts at: 1563 3744 4117 1563
Size: 2181 373 3063
Fragments arranged by size:
3063 2181 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2688 3680 5064 5332 1632
Size: 1056 992 1384 268 1917
Fragments arranged by size:
1917 1384 1056 992 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5420
AflIII A'CryG_T
Cuts at: 1203 4516 5493 1203
Size: 3313 977 1327
Fragments arranged by size:
3313 1327 977
AgeI A'CCGG_T
Cuts at: 4448 4448
Size: 5617
AhdI GACnn_n'nnGTC
Cuts at: 4346 4346
Size: 5617
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5617
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5617
AlwNI CAG_nnn'CTG
Cuts at: 3360 4932 3360
Size: 1572 4045
ApaI G_GGCC'C
Cuts at: 2540 2540
Size: 5617
ApaLI G'TGCA_C
Cuts at: 3674 4830 3674
Size: 1156 4461
ApoI r'AATT_y
Cuts at: 1493 2249 2926 3152 3304 5413 1493
Size: 756 677 226 152 2109 1697
Fragments arranged by size:
2109 1697 756 677 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4171 160
Size: 872 3139 1606
Fragments arranged by size:
3139 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5617
AvaI C'yCGr_G
Cuts at: 1870 1943 2410 2942 1870
Size: 73 467 532 4545
Fragments arranged by size:
4545 532 467 73
BamHI G'GATC_C
Cuts at: 2947 3314 2947
Size: 367 5250
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2699 2938 4393 618
Size: 325 217 627 912 239 1455 1842
Fragments arranged by size:
1842 1455 912 627 325 239 217
BanII G_rGCy'C
Cuts at: 1391 2109 2319 2331 2540 2936 1391
Size: 718 210 12 209 396 4072
Fragments arranged by size:
4072 718 396 210 209 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2001 2676 2835 928
Size: 971 102 675 159 3710
Fragments arranged by size:
3710 971 675 159 102
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3808 3842 1903
Size: 34 1871 34 3678
Fragments arranged by size:
3678 1871 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2498 3509 4719 2498
Size: 1011 1210 3396
Fragments arranged by size:
3396 1210 1011
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5617
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4228 136
Size: 107 122 71 631 605 2556 1525
Fragments arranged by size:
2556 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5612 5612
Size: 5617
BlpI GC'TnA_GC
Cuts at: 2837 3034 2837
Size: 197 5420
Bme1580I G_kGCm'C
Cuts at: 1790 2540 2702 3678 4834 1790
Size: 750 162 976 1156 2573
Fragments arranged by size:
2573 1156 976 750 162
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5617
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2604 3348 4306
Size: 416 53 83 399 24 1187 744 958
Cuts at: 4306 442
Size: 1753
Fragments arranged by size:
1753 1187 958 744 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5617
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2714 4277 1239
Size: 1475 1563 2579
Fragments arranged by size:
2579 1563 1475
Bpu10I CC'TnA_GC
Cuts at: 2229 2229
Size: 5617
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2456 3742 4607 4905 2456
Size: 1286 865 298 3168
Fragments arranged by size:
3168 1286 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4280 882
Size: 617 2781 2219
Fragments arranged by size:
2781 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2494 3408 493
Size: 711 1290 914 2702
Fragments arranged by size:
2702 1290 914 711
BsaBI GATnn'nnATC
Cuts at: 5611 5611
Size: 5617
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3424 3806 4441 275
Size: 53 83 186 936 1891 382 635 1451
Fragments arranged by size:
1891 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4050 4448 4722 4869 5280 4050
Size: 398 274 147 411 4387
Fragments arranged by size:
4387 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2255 2285 2306 2336 2406
Size: 30 165 30 852 30 21 30 70
Cuts at: 2406 2436 1178
Size: 30 4359
Fragments arranged by size:
4359 852 165 70 30 30 30 30
30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5617
BseYI C'CCAG_C
Cuts at: 2067 2330 4820 5316 2067
Size: 263 2490 496 2368
Fragments arranged by size:
2490 2368 496 263
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2168 1163
Size: 573 432 4612
Fragments arranged by size:
4612 573 432
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3067 3828 4856 1540
Size: 275 186 1066 761 1028 2301
Fragments arranged by size:
2301 1066 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2319 3678 3763 4834 2319
Size: 1359 85 1071 3102
Fragments arranged by size:
3102 1359 1071 85
BsmI GAATG_Cn'
Cuts at: 3128 3221 3128
Size: 93 5524
BsmBI CGTCTCn'nnnn_
Cuts at: 2714 2714
Size: 5617
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2319 2331 2540 2702 2936 3678
Size: 399 319 210 12 209 162 234 742
Cuts at: 3678 3763 4834 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 319 234 210 209
162 85 12
BspEI T'CCGG_A
Cuts at: 5280 5280
Size: 5617
BspHI T'CATG_A
Cuts at: 3506 5236 3506
Size: 1730 3887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2972 844
Size: 2128 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2233 3504 1394
Size: 429 410 1271 3507
Fragments arranged by size:
3507 1271 429 410
BsrDI GCAATG_nn'
Cuts at: 65 1248 4112 4286 65
Size: 1183 2864 174 1396
Fragments arranged by size:
2864 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4261 4448 1700
Size: 110 2451 187 2869
Fragments arranged by size:
2869 2451 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5617
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5617
BssSI C'ACGA_G
Cuts at: 3677 4689 3677
Size: 1012 4605
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5617
BstBI TT'CG_AA
Cuts at: 4421 4421
Size: 5617
BstEII G'GTnAC_C
Cuts at: 1184 2422 1184
Size: 1238 4379
BstYI r'GATC_y
Cuts at: 1972 2344 2483 2947 3314 3699 3716 5168 5254
Size: 372 139 464 367 385 17 1452 86
Cuts at: 5254 5266 5612 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 464 385 372 367 346 139
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2208 513
Size: 552 1143 3922
Fragments arranged by size:
3922 1143 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4878
Fragments arranged by size:
4878 683 56
BtsI GCAGTG_nn'
Cuts at: 2445 2757 3133 3927 3947 2445
Size: 312 376 794 20 4115
Fragments arranged by size:
4115 794 376 312 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5617
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5582
DraI TTT'AAA
Cuts at: 2921 2921
Size: 5617
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4624 809
Size: 507 3308 1802
Fragments arranged by size:
3308 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3064 3953 5582
Size: 54 1425 50 275 18 1234 889 1629
Cuts at: 5582 8
Size: 43
Fragments arranged by size:
1629 1425 1234 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3064 1537
Size: 275 1252 4090
Fragments arranged by size:
4090 1252 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2840 3547 1353
Size: 1487 707 3423
Fragments arranged by size:
3423 1487 707
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2537 4204 4578 4724 387
Size: 1064 138 948 1667 374 146 1280
Fragments arranged by size:
1667 1280 1064 948 374 146 138
EcoICRI GAG'CTC
Cuts at: 2317 2317
Size: 5617
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2688 2714 3680 4277 5064 5332 1239
Size: 393 1056 26 966 597 787 268 1524
Fragments arranged by size:
1524 1056 966 787 597 393 268 26
EcoO109I rG'GnC_Cy
Cuts at: 2537 2537
Size: 5617
EcoRI G'AATT_C
Cuts at: 2926 3304 2926
Size: 378 5239
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2216 2443 2216
Size: 227 5390
FspI TGC'GCA
Cuts at: 4123 4123
Size: 5617
HaeII r_GCGC'y
Cuts at: 1678 2067 4764 1678
Size: 389 2697 2531
Fragments arranged by size:
2697 2531 389
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2255 2287 2306 2338 2468
Size: 32 135 32 683 32 19 32 130
Cuts at: 2468 2500 4258 4290 4332 4364 1373
Size: 32 1758 32 42 32 2626
Fragments arranged by size:
2626 1758 683 135 130 42 32 32
32 32 32 32 32 19
HincII GTy'rAC
Cuts at: 669 1657 1938 2961 3207 669
Size: 988 281 1023 246 3079
Fragments arranged by size:
3079 1023 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5617
HpaI GTT'AAC
Cuts at: 3207 3207
Size: 5617
KpnI G_GTAC'C
Cuts at: 2942 2942
Size: 5617
MfeI C'AATT_G
Cuts at: 3216 3216
Size: 5617
MluI A'CGCG_T
Cuts at: 5493 5493
Size: 5617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4731 4915 1305
Size: 3426 184 2007
Fragments arranged by size:
3426 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3795
Fragments arranged by size:
3795 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2569 3575 3934 4093 518
Size: 648 1403 1006 359 159 2042
Fragments arranged by size:
2042 1403 1006 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2182 2295 3033 3710 4858 5103 1606
Size: 576 113 738 677 1148 245 2120
Fragments arranged by size:
2120 1148 738 677 576 245 113
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5065
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5617
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5617
NotI GC'GGCC_GC
Cuts at: 3064 3064
Size: 5617
NspI r_CATG'y
Cuts at: 1132 1229 2461 2975 4520 1132
Size: 97 1232 514 1545 2229
Fragments arranged by size:
2229 1545 1232 514 97
PasI CC'CwG_GG
Cuts at: 1348 2533 1348
Size: 1185 4432
PciI A'CATG_T
Cuts at: 4516 4516
Size: 5617
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2116 1108
Size: 1008 4609
PfoI T'CCnGG_A
Cuts at: 2259 2392 2259
Size: 133 5484
PmeI GTTT'AAAC
Cuts at: 2921 2921
Size: 5617
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3686 3718 5199 5231 3686
Size: 32 1481 32 4072
Fragments arranged by size:
4072 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5617
PsiI TTA'TAA
Cuts at: 3187 3187
Size: 5617
PspOMI G'GGCC_C
Cuts at: 2536 2536
Size: 5617
PstI C_TGCA'G
Cuts at: 830 2689 2969 830
Size: 1859 280 3478
Fragments arranged by size:
3478 1859 280
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5617
PvuII CAG'CTG
Cuts at: 2182 2295 2182
Size: 113 5504
SacI G_AGCT'C
Cuts at: 2319 2319
Size: 5617
SalI G'TCGA_C
Cuts at: 1936 2959 1936
Size: 1023 4594
SbfI CC_TGCA'GG
Cuts at: 2969 2969
Size: 5617
ScaI AGT'ACT
Cuts at: 3865 5577 3865
Size: 1712 3905
SfcI C'TryA_G
Cuts at: 826 1045 2685 2965 4100 4781 4972 826
Size: 219 1640 280 1135 681 191 1471
Fragments arranged by size:
1640 1471 1135 681 280 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5617
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5617
SmaI CCC'GGG
Cuts at: 1872 2944 1872
Size: 1072 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2471 3721 4622 4884 820
Size: 197 926 528 1250 901 262 1553
Fragments arranged by size:
1553 1250 926 901 528 262 197
SnaBI TAC'GTA
Cuts at: 493 3408 493
Size: 2915 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5617
SphI G_CATG'C
Cuts at: 1132 2975 1132
Size: 1843 3774
SspI AAT'ATT
Cuts at: 5 52 3541 5
Size: 47 3489 2081
Fragments arranged by size:
3489 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5617
StyI C'CwwG_G
Cuts at: 513 1065 3056 513
Size: 552 1991 3074
Fragments arranged by size:
3074 1991 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2389 3644 3661 3814 3999 4444 325
Size: 1255 809 1255 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1255 809 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3863 5575 96
Size: 274 80 33 51 1443 1886 1712 138
Fragments arranged by size:
1886 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2388 2406 2420 2438 5577 5609 2388
Size: 18 14 18 3139 32 2396
Fragments arranged by size:
3139 2396 32 18 18 14
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5617
XbaI T'CTAG_A
Cuts at: 2440 2953 2440
Size: 513 5104
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2159 2338 2159
Size: 179 5438
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5617
XmaI C'CCGG_G
Cuts at: 1870 2942 1870
Size: 1072 4545
XmnI GAAnn'nnTTC
Cuts at: 3746 3746
Size: 5617
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3425 276
Size: 53 83 186 936 1891 2468
Fragments arranged by size:
2468 1891 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI