Restriction map for
pFN21AE1791
(Circular) (Six-base) MAPSORT of: pFN21AE1791.seq Check: 2690 from: 1 to: 5614
pFN21AE1791 5614 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3424 278
Size: 53 83 186 936 1888 2468
Fragments arranged by size:
2468 1888 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2957 1937
Size: 1020 4594
Acc65I G'GTAC_C
Cuts at: 2935 2935
Size: 5614
AclI AA'CG_TT
Cuts at: 1563 3741 4114 1563
Size: 2178 373 3063
Fragments arranged by size:
3063 2178 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3677 5061 5329 1632
Size: 2045 1384 268 1917
Fragments arranged by size:
2045 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5417
AflIII A'CryG_T
Cuts at: 1203 4513 5490 1203
Size: 3310 977 1327
Fragments arranged by size:
3310 1327 977
AgeI A'CCGG_T
Cuts at: 4445 4445
Size: 5614
AhdI GACnn_n'nnGTC
Cuts at: 2313 4343 2313
Size: 2030 3584
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5614
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5614
AlwNI CAG_nnn'CTG
Cuts at: 2084 2612 3357 4929 2084
Size: 528 745 1572 2769
Fragments arranged by size:
2769 1572 745 528
ApaLI G'TGCA_C
Cuts at: 3671 4827 3671
Size: 1156 4458
ApoI r'AATT_y
Cuts at: 1493 2923 3149 3301 5410 1493
Size: 1430 226 152 2109 1697
Fragments arranged by size:
2109 1697 1430 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4168 160
Size: 872 3136 1606
Fragments arranged by size:
3136 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5614
AvaI C'yCGr_G
Cuts at: 1870 1943 2939 1870
Size: 73 996 4545
Fragments arranged by size:
4545 996 73
BamHI G'GATC_C
Cuts at: 2944 3311 2944
Size: 367 5247
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2935 4390 618
Size: 325 217 627 1148 1455 1842
Fragments arranged by size:
1842 1455 1148 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2405 2879 2933 1391
Size: 1014 474 54 4072
Fragments arranged by size:
4072 1014 474 54
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4643
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3805 3839 1903
Size: 34 1868 34 3678
Fragments arranged by size:
3678 1868 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3506 4716 3506
Size: 1210 4404
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5614
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4225 136
Size: 107 122 71 631 605 2553 1525
Fragments arranged by size:
2553 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5609 5609
Size: 5614
BlpI GC'TnA_GC
Cuts at: 3031 3031
Size: 5614
Bme1580I G_kGCm'C
Cuts at: 1790 3675 4831 1790
Size: 1885 1156 2573
Fragments arranged by size:
2573 1885 1156
BmgBI CAC'GTC
Cuts at: 1318 2203 1318
Size: 885 4729
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3345 4303 442
Size: 416 53 83 399 24 1928 958 1753
Fragments arranged by size:
1928 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5614
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2236 2524 2606 2812 4274 1239
Size: 997 288 82 206 1462 2579
Fragments arranged by size:
2579 1462 997 288 206 82
Bpu10I CC'TnA_GC
Cuts at: 2404 2404
Size: 5614
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2855 3739 4604 4902 2855
Size: 884 865 298 3567
Fragments arranged by size:
3567 884 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2882 4277 882
Size: 617 1383 1395 2219
Fragments arranged by size:
2219 1395 1383 617
BsaAI yAC'GTr
Cuts at: 493 1204 3405 493
Size: 711 2201 2702
Fragments arranged by size:
2702 2201 711
BsaBI GATnn'nnATC
Cuts at: 5608 5608
Size: 5614
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3421 3803 4438 275
Size: 53 83 186 936 1888 382 635 1451
Fragments arranged by size:
1888 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4047 4445 4719 4866 5277 4047
Size: 398 274 147 411 4384
Fragments arranged by size:
4384 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2497 2527 1178
Size: 30 165 30 1094 30 4265
Fragments arranged by size:
4265 1094 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2645 2680 2747 1186
Size: 1459 35 67 4053
Fragments arranged by size:
4053 1459 67 35
BseYI C'CCAG_C
Cuts at: 2483 2704 2855 4817 5313 2483
Size: 221 151 1962 496 2784
Fragments arranged by size:
2784 1962 496 221 151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5041
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3064 3825 4853 1540
Size: 275 186 1063 761 1028 2301
Fragments arranged by size:
2301 1063 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2405 2625 2851 3675 3760 4831 2405
Size: 220 226 824 85 1071 3188
Fragments arranged by size:
3188 1071 824 226 220 85
BsmI GAATG_Cn'
Cuts at: 3125 3218 3125
Size: 93 5521
BsmBI CGTCTCn'nnnn_
Cuts at: 2212 2212
Size: 5614
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2405 2625 2851 2879 2933 3675 3760
Size: 399 615 220 226 28 54 742 85
Cuts at: 3760 4831 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 615 399 226 220 85
54 28
BspEI T'CCGG_A
Cuts at: 5277 5277
Size: 5614
BspHI T'CATG_A
Cuts at: 2631 3503 5233 2631
Size: 872 1730 3012
Fragments arranged by size:
3012 1730 872
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2355 2969 844
Size: 1511 614 3489
Fragments arranged by size:
3489 1511 614
BsrBI CCG'CTC
Cuts at: 1394 1823 2325 3501 1394
Size: 429 502 1176 3507
Fragments arranged by size:
3507 1176 502 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4109 4283 65
Size: 1183 2861 174 1396
Fragments arranged by size:
2861 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2010 2289 2531 4258 4445 1700
Size: 110 200 279 242 1727 187 2869
Fragments arranged by size:
2869 1727 279 242 200 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5614
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5614
BssSI C'ACGA_G
Cuts at: 3674 4686 3674
Size: 1012 4602
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5614
BstBI TT'CG_AA
Cuts at: 4418 4418
Size: 5614
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5614
BstYI r'GATC_y
Cuts at: 1972 2081 2337 2773 2944 3311 3696 3713 5165
Size: 109 256 436 171 367 385 17 1452
Cuts at: 5165 5251 5263 5609 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 436 385 367 346 256 171
109 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2333 2537 2657 2333
Size: 204 120 5290
Fragments arranged by size:
5290 204 120
BtgI C'CryG_G
Cuts at: 513 1065 2004 2136 2233 513
Size: 552 939 132 97 3894
Fragments arranged by size:
3894 939 552 132 97
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4875
Fragments arranged by size:
4875 683 56
BtsI GCAGTG_nn'
Cuts at: 2423 2613 3130 3924 3944 2423
Size: 190 517 794 20 4093
Fragments arranged by size:
4093 794 517 190 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5614
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5579
DraI TTT'AAA
Cuts at: 2918 2918
Size: 5614
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2804 4621 809
Size: 507 1488 1817 1802
Fragments arranged by size:
1817 1802 1488 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3061 3950 5579
Size: 54 1425 50 275 18 1231 889 1629
Cuts at: 5579 8
Size: 43
Fragments arranged by size:
1629 1425 1231 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3061 1537
Size: 275 1249 4090
Fragments arranged by size:
4090 1249 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2315 3544 1353
Size: 962 1229 3423
Fragments arranged by size:
3423 1229 962
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4201 4575 4721 387
Size: 1064 138 2612 374 146 1280
Fragments arranged by size:
2612 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2403 2403
Size: 5614
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2236 2524 2606 2812 3677 4274 5061
Size: 393 604 288 82 206 865 597 787
Cuts at: 5061 5329 1239
Size: 268 1524
Fragments arranged by size:
1524 865 787 604 597 393 288 268
206 82
EcoRI G'AATT_C
Cuts at: 2923 3301 2923
Size: 378 5236
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2280 2344 2280
Size: 64 5550
FseI GG_CCGG'CC
Cuts at: 2014 2014
Size: 5614
FspI TGC'GCA
Cuts at: 4120 4120
Size: 5614
HaeII r_GCGC'y
Cuts at: 1678 4761 1678
Size: 3083 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2299 2331 4255 4287 4329
Size: 32 135 32 727 32 1924 32 42
Cuts at: 4329 4361 1373
Size: 32 2626
Fragments arranged by size:
2626 1924 727 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2958 3204 669
Size: 988 281 1020 246 3079
Fragments arranged by size:
3079 1020 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5614
HpaI GTT'AAC
Cuts at: 3204 3204
Size: 5614
KpnI G_GTAC'C
Cuts at: 2939 2939
Size: 5614
MfeI C'AATT_G
Cuts at: 3213 3213
Size: 5614
MluI A'CGCG_T
Cuts at: 5490 5490
Size: 5614
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2367 4728 4912 1305
Size: 1062 2361 184 2007
Fragments arranged by size:
2361 2007 1062 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3792
Fragments arranged by size:
3792 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3572 3931 4090 518
Size: 648 2406 359 159 2042
Fragments arranged by size:
2406 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2686 3030 3707 4855 5100 1606
Size: 1080 344 677 1148 245 2120
Fragments arranged by size:
2120 1148 1080 677 344 245
NaeI GCC'GGC
Cuts at: 2012 2012
Size: 5614
NcoI C'CATG_G
Cuts at: 513 1065 2004 2136 513
Size: 552 939 132 3991
Fragments arranged by size:
3991 939 552 132
NdeI CA'TA_TG
Cuts at: 387 2740 387
Size: 2353 3261
NgoMIV G'CCGG_C
Cuts at: 2010 2010
Size: 5614
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5614
NotI GC'GGCC_GC
Cuts at: 3061 3061
Size: 5614
NspI r_CATG'y
Cuts at: 1132 1229 2972 4517 1132
Size: 97 1743 1545 2229
Fragments arranged by size:
2229 1743 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2178 1348
Size: 830 4784
PciI A'CATG_T
Cuts at: 4513 4513
Size: 5614
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5614
PfoI T'CCnGG_A
Cuts at: 2213 2213
Size: 5614
PmeI GTTT'AAAC
Cuts at: 2918 2918
Size: 5614
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3683 3715 5196 5228 3683
Size: 32 1481 32 4069
Fragments arranged by size:
4069 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5614
PsiI TTA'TAA
Cuts at: 3184 3184
Size: 5614
PstI C_TGCA'G
Cuts at: 830 2064 2352 2966 830
Size: 1234 288 614 3478
Fragments arranged by size:
3478 1234 614 288
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5614
SacI G_AGCT'C
Cuts at: 2405 2405
Size: 5614
SalI G'TCGA_C
Cuts at: 1936 2956 1936
Size: 1020 4594
SapI GCTCTTCn'nnn_
Cuts at: 2315 2315
Size: 5614
SbfI CC_TGCA'GG
Cuts at: 2966 2966
Size: 5614
ScaI AGT'ACT
Cuts at: 3862 5574 3862
Size: 1712 3902
SfcI C'TryA_G
Cuts at: 826 1045 2060 2248 2348 2962 4097 4778 4969
Size: 219 1015 188 100 614 1135 681 191
Cuts at: 4969 826
Size: 1471
Fragments arranged by size:
1471 1135 1015 681 614 219 191 188
100
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5614
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5614
SmaI CCC'GGG
Cuts at: 1872 2941 1872
Size: 1069 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2870 3718 4619 4881 820
Size: 197 926 927 848 901 262 1553
Fragments arranged by size:
1553 927 926 901 848 262 197
SnaBI TAC'GTA
Cuts at: 493 3405 493
Size: 2912 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5614
SphI G_CATG'C
Cuts at: 1132 2972 1132
Size: 1840 3774
SspI AAT'ATT
Cuts at: 5 52 3538 5
Size: 47 3486 2081
Fragments arranged by size:
3486 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5614
StyI C'CwwG_G
Cuts at: 513 1065 2004 2136 2790 3053 513
Size: 552 939 132 654 263 3074
Fragments arranged by size:
3074 939 654 552 263 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2129 2468 2489 2857 3641 3658 3811
Size: 1255 549 339 21 368 784 17 153
Cuts at: 3811 3996 4441 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 784 549 445 368 339 185
153 21 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2895 3860 5572
Size: 274 80 33 51 1443 918 965 1712
Cuts at: 5572 96
Size: 138
Fragments arranged by size:
1712 1443 965 918 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2225 2257 5574 5606 2225
Size: 32 3317 32 2233
Fragments arranged by size:
3317 2233 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5614
XbaI T'CTAG_A
Cuts at: 2950 2950
Size: 5614
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5614
XmaI C'CCGG_G
Cuts at: 1870 2939 1870
Size: 1069 4545
XmnI GAAnn'nnTTC
Cuts at: 3743 3743
Size: 5614
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3422 276
Size: 53 83 186 936 1888 2468
Fragments arranged by size:
2468 1888 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FalI FseI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoNI
EcoO109I EcoRV FspAI KasI NarI NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI