Restriction map for
pFN21AE1790
(Circular) (Six-base) MAPSORT of: pFN21AE1790.seq Check: 7467 from: 1 to: 5596
pFN21AE1790 5596 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2708 2708
Size: 5596
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3406 278
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2939 1937
Size: 1002 4594
Acc65I G'GTAC_C
Cuts at: 2917 2917
Size: 5596
AclI AA'CG_TT
Cuts at: 1563 3723 4096 1563
Size: 2160 373 3063
Fragments arranged by size:
3063 2160 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3659 5043 5311 1632
Size: 2027 1384 268 1917
Fragments arranged by size:
2027 1917 1384 268
AfeI AGC'GCT
Cuts at: 2789 2789
Size: 5596
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5399
AflIII A'CryG_T
Cuts at: 1203 2370 2475 4495 5472 1203
Size: 1167 105 2020 977 1327
Fragments arranged by size:
2020 1327 1167 977 105
AgeI A'CCGG_T
Cuts at: 4427 4427
Size: 5596
AhdI GACnn_n'nnGTC
Cuts at: 4325 4325
Size: 5596
AleI CACnn'nnGTG
Cuts at: 1166 2744 1166
Size: 1578 4018
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5596
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2462 2494 2462
Size: 32 5564
AlwNI CAG_nnn'CTG
Cuts at: 2624 3339 4911 2624
Size: 715 1572 3309
Fragments arranged by size:
3309 1572 715
ApaLI G'TGCA_C
Cuts at: 2366 3653 4809 2366
Size: 1287 1156 3153
Fragments arranged by size:
3153 1287 1156
ApoI r'AATT_y
Cuts at: 1493 2905 3131 3283 5392 1493
Size: 1412 226 152 2109 1697
Fragments arranged by size:
2109 1697 1412 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4150 160
Size: 872 3118 1606
Fragments arranged by size:
3118 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
AvaI C'yCGr_G
Cuts at: 1870 1943 2168 2185 2306 2921 1870
Size: 73 225 17 121 615 4545
Fragments arranged by size:
4545 615 225 121 73 17
BamHI G'GATC_C
Cuts at: 2926 3293 2926
Size: 367 5229
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2101 2506 2797 2917 4372
Size: 325 217 627 314 405 291 120 1455
Cuts at: 4372 618
Size: 1842
Fragments arranged by size:
1842 1455 627 405 325 314 291 217
120
BanII G_rGCy'C
Cuts at: 1391 2736 2915 1391
Size: 1345 179 4072
Fragments arranged by size:
4072 1345 179
BbeI G_GCGC'C
Cuts at: 2105 2105
Size: 5596
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4625
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2395 2429 3787 3821 1903
Size: 34 50 34 374 34 1358 34 3678
Fragments arranged by size:
3678 1358 374 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3488 4698 3488
Size: 1210 4386
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5596
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2882 4207
Size: 107 122 71 631 605 337 873 1325
Cuts at: 4207 136
Size: 1525
Fragments arranged by size:
1525 1325 873 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 5591 5591
Size: 5596
BlpI GC'TnA_GC
Cuts at: 3013 3013
Size: 5596
Bme1580I G_kGCm'C
Cuts at: 1790 2169 2370 2511 2682 2802 3657 4813 1790
Size: 379 201 141 171 120 855 1156 2573
Fragments arranged by size:
2573 1156 855 379 201 171 141 120
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5596
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3327 4285 442
Size: 416 53 83 399 24 1910 958 1753
Fragments arranged by size:
1910 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5596
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2377 2377
Size: 5596
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2473 2561 2729 2804 4256 1239
Size: 1234 88 168 75 1452 2579
Fragments arranged by size:
2579 1452 1234 168 88 75
Bpu10I CC'TnA_GC
Cuts at: 2486 2486
Size: 5596
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2213 2855 3721 4586 4884 2213
Size: 642 866 865 298 2925
Fragments arranged by size:
2925 866 865 642 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4259 882
Size: 617 2760 2219
Fragments arranged by size:
2760 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2248 2641 2671 2746 3387 493
Size: 711 1044 393 30 75 641 2702
Fragments arranged by size:
2702 1044 711 641 393 75 30
BsaBI GATnn'nnATC
Cuts at: 5590 5590
Size: 5596
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2102 3403 3785 4420
Size: 53 83 186 936 569 1301 382 635
Cuts at: 4420 275
Size: 1451
Fragments arranged by size:
1451 1301 936 635 569 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4029 4427 4701 4848 5259 4029
Size: 398 274 147 411 4366
Fragments arranged by size:
4366 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5371
Fragments arranged by size:
5371 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5596
BseYI C'CCAG_C
Cuts at: 2050 2113 4799 5295 2050
Size: 63 2686 496 2351
Fragments arranged by size:
2686 2351 496 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2575 1163
Size: 573 839 4184
Fragments arranged by size:
4184 839 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2061 2332 2419 3046 3807 4835
Size: 275 186 60 271 87 627 761 1028
Cuts at: 4835 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 627 275 271 186 87
60
BsiHKAI G_wGCw'C
Cuts at: 2370 2736 3657 3742 4813 2370
Size: 366 921 85 1071 3153
Fragments arranged by size:
3153 1071 921 366 85
BsiWI C'GTAC_G
Cuts at: 2244 2244
Size: 5596
BsmI GAATG_Cn'
Cuts at: 2530 3107 3200 2530
Size: 577 93 4926
Fragments arranged by size:
4926 577 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2318 2318
Size: 5596
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2169 2370 2511 2682 2736 2802 2915
Size: 399 379 201 141 171 54 66 113
Cuts at: 2915 3657 3742 4813 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 379 201 171 141
113 85 66 54
BspEI T'CCGG_A
Cuts at: 5259 5259
Size: 5596
BspHI T'CATG_A
Cuts at: 3485 5215 3485
Size: 1730 3866
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2708 2951 844
Size: 1864 243 3489
Fragments arranged by size:
3489 1864 243
BsrBI CCG'CTC
Cuts at: 1394 1823 2818 3483 1394
Size: 429 995 665 3507
Fragments arranged by size:
3507 995 665 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4091 4265 65
Size: 1183 2843 174 1396
Fragments arranged by size:
2843 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2021 2118 2331 4240 4427 1700
Size: 110 211 97 213 1909 187 2869
Fragments arranged by size:
2869 1909 213 211 187 110 97
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5596
BssHII G'CGCG_C
Cuts at: 1925 2277 1925
Size: 352 5244
BssSI C'ACGA_G
Cuts at: 3656 4668 3656
Size: 1012 4584
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5596
BstBI TT'CG_AA
Cuts at: 4400 4400
Size: 5596
BstEII G'GTnAC_C
Cuts at: 1184 2423 1184
Size: 1239 4357
BstXI CCAn_nnnn'nTGG
Cuts at: 2782 2782
Size: 5596
BstYI r'GATC_y
Cuts at: 1972 2926 3293 3678 3695 5147 5233 5245 5591
Size: 954 367 385 17 1452 86 12 346
Cuts at: 5591 1972
Size: 1977
Fragments arranged by size:
1977 1452 954 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2136 2179 2272 2317 2401 2419 2466
Size: 552 1071 43 93 45 84 18 47
Cuts at: 2466 513
Size: 3643
Fragments arranged by size:
3643 1071 552 93 84 47 45 43
18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4857
Fragments arranged by size:
4857 683 56
BtsI GCAGTG_nn'
Cuts at: 2413 2682 3112 3906 3926 2413
Size: 269 430 794 20 4083
Fragments arranged by size:
4083 794 430 269 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5596
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5561
DraI TTT'AAA
Cuts at: 2900 2900
Size: 5596
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2310 4603 809
Size: 507 994 2293 1802
Fragments arranged by size:
2293 1802 994 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2196 2430 3043
Size: 54 1425 50 275 18 366 234 613
Cuts at: 3043 3932 5561 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 613 366 275 234 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3043 1537
Size: 275 1231 4090
Fragments arranged by size:
4090 1231 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2609 3526 1353
Size: 1256 917 3423
Fragments arranged by size:
3423 1256 917
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4183 4557 4703 387
Size: 1064 138 2594 374 146 1280
Fragments arranged by size:
2594 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2734 2734
Size: 5596
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2473 2561 2729 2804 3659 4256 5043
Size: 393 841 88 168 75 855 597 787
Cuts at: 5043 5311 1239
Size: 268 1524
Fragments arranged by size:
1524 855 841 787 597 393 268 168
88 75
EcoO109I rG'GnC_Cy
Cuts at: 2174 2174
Size: 5596
EcoRI G'AATT_C
Cuts at: 2905 3283 2905
Size: 378 5218
FseI GG_CCGG'CC
Cuts at: 2025 2025
Size: 5596
FspI TGC'GCA
Cuts at: 4102 4102
Size: 5596
HaeII r_GCGC'y
Cuts at: 1678 2015 2105 2791 4743 1678
Size: 337 90 686 1952 2531
Fragments arranged by size:
2531 1952 686 337 90
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2618 2650 4237 4269 4311
Size: 32 135 32 1046 32 1587 32 42
Cuts at: 4311 4343 1373
Size: 32 2626
Fragments arranged by size:
2626 1587 1046 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2940 3186 669
Size: 988 281 1002 246 3079
Fragments arranged by size:
3079 1002 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5596
HpaI GTT'AAC
Cuts at: 3186 3186
Size: 5596
KasI G'GCGC_C
Cuts at: 2101 2101
Size: 5596
KpnI G_GTAC'C
Cuts at: 2921 2921
Size: 5596
MfeI C'AATT_G
Cuts at: 3195 3195
Size: 5596
MluI A'CGCG_T
Cuts at: 2370 5472 2370
Size: 3102 2494
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4710 4894 1305
Size: 3405 184 2007
Fragments arranged by size:
3405 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2198 10
Size: 54 1425 343 366 3408
Fragments arranged by size:
3408 1425 366 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2744 3554 3913 4072 518
Size: 648 1578 810 359 159 2042
Fragments arranged by size:
2042 1578 810 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2181 2274 2299 2319 2403 2421 2451 3012
Size: 575 93 25 20 84 18 30 561
Cuts at: 3012 3689 4837 5082 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 575 561 245 93 84
30 25 20 18
NaeI GCC'GGC
Cuts at: 2023 2120 2023
Size: 97 5499
NarI GG'CG_CC
Cuts at: 2102 2102
Size: 5596
NcoI C'CATG_G
Cuts at: 513 1065 2466 513
Size: 552 1401 3643
Fragments arranged by size:
3643 1401 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5596
NgoMIV G'CCGG_C
Cuts at: 2021 2118 2021
Size: 97 5499
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5596
NotI GC'GGCC_GC
Cuts at: 3043 3043
Size: 5596
NspI r_CATG'y
Cuts at: 1132 1229 2954 4499 1132
Size: 97 1725 1545 2229
Fragments arranged by size:
2229 1725 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5596
PciI A'CATG_T
Cuts at: 4495 4495
Size: 5596
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5596
PfoI T'CCnGG_A
Cuts at: 2706 2706
Size: 5596
PmeI GTTT'AAAC
Cuts at: 2900 2900
Size: 5596
PmlI CAC'GTG
Cuts at: 2671 2671
Size: 5596
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3665 3697 5178 5210 3665
Size: 32 1481 32 4051
Fragments arranged by size:
4051 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2174 2174
Size: 5596
PshAI GACnn'nnGTC
Cuts at: 1613 2421 1613
Size: 808 4788
PsiI TTA'TAA
Cuts at: 3166 3166
Size: 5596
PstI C_TGCA'G
Cuts at: 830 2948 830
Size: 2118 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5596
PvuII CAG'CTG
Cuts at: 2299 2299
Size: 5596
SacI G_AGCT'C
Cuts at: 2736 2736
Size: 5596
SacII CC_GC'GG
Cuts at: 2182 2275 2320 2404 2422 2182
Size: 93 45 84 18 5356
Fragments arranged by size:
5356 93 84 45 18
SalI G'TCGA_C
Cuts at: 1936 2938 1936
Size: 1002 4594
SanDI GG'GwC_CC
Cuts at: 2174 2174
Size: 5596
SbfI CC_TGCA'GG
Cuts at: 2948 2948
Size: 5596
ScaI AGT'ACT
Cuts at: 3844 5556 3844
Size: 1712 3884
SexAI A'CCwGG_T
Cuts at: 2649 2649
Size: 5596
SfcI C'TryA_G
Cuts at: 826 1045 2944 4079 4760 4951 826
Size: 219 1899 1135 681 191 1471
Fragments arranged by size:
1899 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2103 2103
Size: 5596
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5596
SmaI CCC'GGG
Cuts at: 1872 2170 2187 2923 1872
Size: 298 17 736 4545
Fragments arranged by size:
4545 736 298 17
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2228 2870 3700 4601 4863 820
Size: 197 926 285 642 830 901 262 1553
Fragments arranged by size:
1553 926 901 830 642 285 262 197
SnaBI TAC'GTA
Cuts at: 493 2641 3387 493
Size: 2148 746 2702
Fragments arranged by size:
2702 2148 746
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5596
SphI G_CATG'C
Cuts at: 1132 2954 1132
Size: 1822 3774
SrfI GCCC'GGGC
Cuts at: 2187 2187
Size: 5596
SspI AAT'ATT
Cuts at: 5 52 3520 5
Size: 47 3468 2081
Fragments arranged by size:
3468 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5596
StyI C'CwwG_G
Cuts at: 513 1065 2466 3035 513
Size: 552 1401 569 3074
Fragments arranged by size:
3074 1401 569 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2042 2275 3623 3640 3793 3978 4423
Size: 1255 462 233 1348 17 153 185 445
Cuts at: 4423 325
Size: 1498
Fragments arranged by size:
1498 1348 1255 462 445 233 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3842 5554 96
Size: 274 80 33 51 1443 1865 1712 138
Fragments arranged by size:
1865 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5556 5588 5556
Size: 32 5564
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5596
XbaI T'CTAG_A
Cuts at: 2932 2932
Size: 5596
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2761 2761
Size: 5596
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5596
XmaI C'CCGG_G
Cuts at: 1870 2168 2185 2921 1870
Size: 298 17 736 4545
Fragments arranged by size:
4545 736 298 17
XmnI GAAnn'nnTTC
Cuts at: 3725 3725
Size: 5596
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3404 276
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbvCI BfrBI BstZ17I Bsu36I
DraIII EcoNI EcoRV FalI FspAI NruI NsiI PacI
PspOMI PspXI PsrI RsrII SapI SfiI SwaI