Restriction map for
pFN21AE1788
(Circular) (Six-base) MAPSORT of: pFN21AE1788.seq Check: 6125 from: 1 to: 5590
pFN21AE1788 5590 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3400 278
Size: 53 83 186 936 1864 2468
Fragments arranged by size:
2468 1864 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2933 1937
Size: 996 4594
Acc65I G'GTAC_C
Cuts at: 2911 2911
Size: 5590
AclI AA'CG_TT
Cuts at: 1563 3717 4090 1563
Size: 2154 373 3063
Fragments arranged by size:
3063 2154 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2279 2815 3653 5037 5305 1632
Size: 647 536 838 1384 268 1917
Fragments arranged by size:
1917 1384 838 647 536 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5393
AflIII A'CryG_T
Cuts at: 1203 4489 5466 1203
Size: 3286 977 1327
Fragments arranged by size:
3286 1327 977
AgeI A'CCGG_T
Cuts at: 4421 4421
Size: 5590
AhdI GACnn_n'nnGTC
Cuts at: 2714 4319 2714
Size: 1605 3985
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5590
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5590
AlwNI CAG_nnn'CTG
Cuts at: 2257 3333 4905 2257
Size: 1076 1572 2942
Fragments arranged by size:
2942 1572 1076
ApaI G_GGCC'C
Cuts at: 2480 2480
Size: 5590
ApaLI G'TGCA_C
Cuts at: 2571 3647 4803 2571
Size: 1076 1156 3358
Fragments arranged by size:
3358 1156 1076
ApoI r'AATT_y
Cuts at: 1493 2222 2899 3125 3277 5386 1493
Size: 729 677 226 152 2109 1697
Fragments arranged by size:
2109 1697 729 677 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4144 160
Size: 872 3112 1606
Fragments arranged by size:
3112 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5590
AvaI C'yCGr_G
Cuts at: 1870 1943 2915 1870
Size: 73 972 4545
Fragments arranged by size:
4545 972 73
BamHI G'GATC_C
Cuts at: 2672 2920 3287 2672
Size: 248 367 4975
Fragments arranged by size:
4975 367 248
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2586 2911 4366 618
Size: 325 217 627 799 325 1455 1842
Fragments arranged by size:
1842 1455 799 627 325 325 217
BanII G_rGCy'C
Cuts at: 1391 2369 2480 2909 1391
Size: 978 111 429 4072
Fragments arranged by size:
4072 978 429 111
BbeI G_GCGC'C
Cuts at: 2590 2590
Size: 5590
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2640 2821 928
Size: 971 741 181 3697
Fragments arranged by size:
3697 971 741 181
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3781 3815 1903
Size: 34 1844 34 3678
Fragments arranged by size:
3678 1844 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3482 4692 3482
Size: 1210 4380
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5590
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2100 2109 4201
Size: 107 122 71 631 605 428 9 2092
Cuts at: 4201 136
Size: 1525
Fragments arranged by size:
2092 1525 631 605 428 122 107 71
9
BglII A'GATC_T
Cuts at: 5585 5585
Size: 5590
BlpI GC'TnA_GC
Cuts at: 3007 3007
Size: 5590
Bme1580I G_kGCm'C
Cuts at: 1790 2480 2540 2575 3651 4807 1790
Size: 690 60 35 1076 1156 2573
Fragments arranged by size:
2573 1156 1076 690 60 35
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5590
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2441 3321 4279
Size: 416 53 83 399 24 1024 880 958
Cuts at: 4279 442
Size: 1753
Fragments arranged by size:
1753 1024 958 880 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5590
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2507 4250 1239
Size: 1268 1743 2579
Fragments arranged by size:
2579 1743 1268
Bpu10I CC'TnA_GC
Cuts at: 2630 2630
Size: 5590
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3715 4580 4878 3715
Size: 865 298 4427
Fragments arranged by size:
4427 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4253 882
Size: 617 2754 2219
Fragments arranged by size:
2754 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3381 493
Size: 711 2177 2702
Fragments arranged by size:
2702 2177 711
BsaBI GATnn'nnATC
Cuts at: 5584 5584
Size: 5590
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2587 3397 3779 4414
Size: 53 83 186 936 1054 810 382 635
Cuts at: 4414 275
Size: 1451
Fragments arranged by size:
1451 1054 936 810 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4023 4421 4695 4842 5253 4023
Size: 398 274 147 411 4360
Fragments arranged by size:
4360 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5365
Fragments arranged by size:
5365 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2005 2025 2088 1186
Size: 819 20 63 4688
Fragments arranged by size:
4688 819 63 20
BseYI C'CCAG_C
Cuts at: 2085 2510 4793 5289 2085
Size: 425 2283 496 2386
Fragments arranged by size:
2386 2283 496 425
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2146 1163
Size: 573 410 4607
Fragments arranged by size:
4607 573 410
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3040 3801 4829 1540
Size: 275 186 1039 761 1028 2301
Fragments arranged by size:
2301 1039 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2575 3651 3736 4807 2575
Size: 1076 85 1071 3358
Fragments arranged by size:
3358 1076 1071 85
BsmI GAATG_Cn'
Cuts at: 2453 3101 3194 2453
Size: 648 93 4849
Fragments arranged by size:
4849 648 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2369 2480 2540 2575 2909 3651 3736
Size: 399 579 111 60 35 334 742 85
Cuts at: 3736 4807 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 579 399 334 111 85
60 35
BspEI T'CCGG_A
Cuts at: 5253 5253
Size: 5590
BspHI T'CATG_A
Cuts at: 2841 3479 5209 2841
Size: 638 1730 3222
Fragments arranged by size:
3222 1730 638
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2564 2945 844
Size: 1720 381 3489
Fragments arranged by size:
3489 1720 381
BsrBI CCG'CTC
Cuts at: 1394 1823 2120 3477 1394
Size: 429 297 1357 3507
Fragments arranged by size:
3507 1357 429 297
BsrDI GCAATG_nn'
Cuts at: 65 1248 2550 4085 4259 65
Size: 1183 1302 1535 174 1396
Fragments arranged by size:
1535 1396 1302 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4234 4421 1700
Size: 110 2424 187 2869
Fragments arranged by size:
2869 2424 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5590
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5590
BssSI C'ACGA_G
Cuts at: 3650 4662 3650
Size: 1012 4578
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2279 2579 794
Size: 1485 300 3805
Fragments arranged by size:
3805 1485 300
BstBI TT'CG_AA
Cuts at: 4394 4394
Size: 5590
BstEII G'GTnAC_C
Cuts at: 1184 2134 1184
Size: 950 4640
BstYI r'GATC_y
Cuts at: 1972 2672 2920 3287 3672 3689 5141 5227 5239
Size: 700 248 367 385 17 1452 86 12
Cuts at: 5239 5585 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 700 385 367 346 248 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2105 513
Size: 552 1040 3998
Fragments arranged by size:
3998 1040 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4851
Fragments arranged by size:
4851 683 56
BtsI GCAGTG_nn'
Cuts at: 2523 2794 3106 3900 3920 2523
Size: 271 312 794 20 4193
Fragments arranged by size:
4193 794 312 271 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5590
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5555
DraI TTT'AAA
Cuts at: 2220 2894 2220
Size: 674 4916
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4597 809
Size: 507 3281 1802
Fragments arranged by size:
3281 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2853 3037 3926
Size: 54 1425 50 275 18 1023 184 889
Cuts at: 3926 5555 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1023 889 275 184 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3037 1537
Size: 275 1225 4090
Fragments arranged by size:
4090 1225 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2813 3520 1353
Size: 1460 707 3423
Fragments arranged by size:
3423 1460 707
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4177 4551 4697 387
Size: 1064 138 2588 374 146 1280
Fragments arranged by size:
2588 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2279 2507 2815 3653 4250 5037 5305
Size: 393 647 228 308 838 597 787 268
Cuts at: 5305 1239
Size: 1524
Fragments arranged by size:
1524 838 787 647 597 393 308 268
228
EcoNI CCTnn'n_nnAGG
Cuts at: 2261 2261
Size: 5590
EcoO109I rG'GnC_Cy
Cuts at: 2310 2477 2310
Size: 167 5423
EcoRI G'AATT_C
Cuts at: 2899 3277 2899
Size: 378 5212
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2221 2221
Size: 5590
FspI TGC'GCA
Cuts at: 4096 4096
Size: 5590
HaeII r_GCGC'y
Cuts at: 1678 2590 4737 1678
Size: 912 2147 2531
Fragments arranged by size:
2531 2147 912
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2700 2732 4231 4263 4305
Size: 32 135 32 1128 32 1499 32 42
Cuts at: 4305 4337 1373
Size: 32 2626
Fragments arranged by size:
2626 1499 1128 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2244 2506 2934 3180 669
Size: 988 281 306 262 428 246 3079
Fragments arranged by size:
3079 988 428 306 281 262 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5590
HpaI GTT'AAC
Cuts at: 3180 3180
Size: 5590
KasI G'GCGC_C
Cuts at: 2586 2586
Size: 5590
KpnI G_GTAC'C
Cuts at: 2915 2915
Size: 5590
MfeI C'AATT_G
Cuts at: 3189 3189
Size: 5590
MluI A'CGCG_T
Cuts at: 5466 5466
Size: 5590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4704 4888 1305
Size: 3399 184 2007
Fragments arranged by size:
3399 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2855 10
Size: 54 1425 343 1023 2745
Fragments arranged by size:
2745 1425 1023 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2409 2543 3548 3907 4066 518
Size: 648 1243 134 1005 359 159 2042
Fragments arranged by size:
2042 1243 1005 648 359 159 134
MspA1I CmG'CkG
Cuts at: 1606 3006 3683 4831 5076 1606
Size: 1400 677 1148 245 2120
Fragments arranged by size:
2120 1400 1148 677 245
NarI GG'CG_CC
Cuts at: 2587 2587
Size: 5590
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5038
NdeI CA'TA_TG
Cuts at: 387 2322 387
Size: 1935 3655
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5590
NotI GC'GGCC_GC
Cuts at: 3037 3037
Size: 5590
NspI r_CATG'y
Cuts at: 1132 1229 2948 4493 1132
Size: 97 1719 1545 2229
Fragments arranged by size:
2229 1719 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2653 1348
Size: 1305 4285
PciI A'CATG_T
Cuts at: 4489 4489
Size: 5590
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5590
PmeI GTTT'AAAC
Cuts at: 2894 2894
Size: 5590
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3659 3691 5172 5204 3659
Size: 32 1481 32 4045
Fragments arranged by size:
4045 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5590
PsiI TTA'TAA
Cuts at: 3160 3160
Size: 5590
PspOMI G'GGCC_C
Cuts at: 2476 2476
Size: 5590
PstI C_TGCA'G
Cuts at: 830 2308 2942 830
Size: 1478 634 3478
Fragments arranged by size:
3478 1478 634
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5590
SalI G'TCGA_C
Cuts at: 1936 2932 1936
Size: 996 4594
SbfI CC_TGCA'GG
Cuts at: 2942 2942
Size: 5590
ScaI AGT'ACT
Cuts at: 3838 5550 3838
Size: 1712 3878
SfcI C'TryA_G
Cuts at: 826 1045 2304 2938 4073 4754 4945 826
Size: 219 1259 634 1135 681 191 1471
Fragments arranged by size:
1471 1259 1135 681 634 219 191
SfoI GGC'GCC
Cuts at: 2588 2588
Size: 5590
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5590
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5590
SmaI CCC'GGG
Cuts at: 1872 2917 1872
Size: 1045 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3694 4595 4857 820
Size: 197 926 1751 901 262 1553
Fragments arranged by size:
1751 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3381 493
Size: 2888 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5590
SphI G_CATG'C
Cuts at: 1132 2948 1132
Size: 1816 3774
SspI AAT'ATT
Cuts at: 5 52 3514 5
Size: 47 3462 2081
Fragments arranged by size:
3462 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5590
StyI C'CwwG_G
Cuts at: 513 1065 2361 3029 513
Size: 552 1296 668 3074
Fragments arranged by size:
3074 1296 668 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3617 3634 3787 3972 4417 325
Size: 1255 2037 17 153 185 445 1498
Fragments arranged by size:
2037 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3836 5548 96
Size: 274 80 33 51 1443 1859 1712 138
Fragments arranged by size:
1859 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5550 5582 5550
Size: 32 5558
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5590
XbaI T'CTAG_A
Cuts at: 2926 2926
Size: 5590
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2530 2530
Size: 5590
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5590
XmaI C'CCGG_G
Cuts at: 1870 2915 1870
Size: 1045 4545
XmnI GAAnn'nnTTC
Cuts at: 2663 3719 2663
Size: 1056 4534
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3398 276
Size: 53 83 186 936 1864 2468
Fragments arranged by size:
2468 1864 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SrfI SwaI