Restriction map for
pFN21AE1787
(Circular) (Six-base) MAPSORT of: pFN21AE1787.seq Check: 647 from: 1 to: 5587
pFN21AE1787 5587 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3397 278
Size: 53 83 186 936 1861 2468
Fragments arranged by size:
2468 1861 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2930 1937
Size: 993 4594
Acc65I G'GTAC_C
Cuts at: 2908 2908
Size: 5587
AclI AA'CG_TT
Cuts at: 1563 3714 4087 1563
Size: 2151 373 3063
Fragments arranged by size:
3063 2151 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2888 3650 5034 5302 1632
Size: 1256 762 1384 268 1917
Fragments arranged by size:
1917 1384 1256 762 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5390
AflIII A'CryG_T
Cuts at: 1203 2524 2560 4486 5463 1203
Size: 1321 36 1926 977 1327
Fragments arranged by size:
1926 1327 1321 977 36
AgeI A'CCGG_T
Cuts at: 4418 4418
Size: 5587
AhdI GACnn_n'nnGTC
Cuts at: 4316 4316
Size: 5587
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5587
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5587
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2693 2725 2693
Size: 32 5555
AlwNI CAG_nnn'CTG
Cuts at: 3330 4902 3330
Size: 1572 4015
ApaLI G'TGCA_C
Cuts at: 3644 4800 3644
Size: 1156 4431
ApoI r'AATT_y
Cuts at: 1493 2008 2299 2483 2825 2896 3122 3274 5383
Size: 515 291 184 342 71 226 152 2109
Cuts at: 5383 1493
Size: 1697
Fragments arranged by size:
2109 1697 515 342 291 226 184 152
71
AseI AT'TA_AT
Cuts at: 160 1032 4141 160
Size: 872 3109 1606
Fragments arranged by size:
3109 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5587
AvaI C'yCGr_G
Cuts at: 1870 1943 2912 1870
Size: 73 969 4545
Fragments arranged by size:
4545 969 73
BamHI G'GATC_C
Cuts at: 2459 2917 3284 2459
Size: 458 367 4762
Fragments arranged by size:
4762 458 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2908 4363 618
Size: 325 217 627 1121 1455 1842
Fragments arranged by size:
1842 1455 1121 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2906 1391
Size: 1515 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2324 2589 928
Size: 971 425 265 3926
Fragments arranged by size:
3926 971 425 265
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3778 3812 1903
Size: 34 1841 34 3678
Fragments arranged by size:
3678 1841 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3479 4689 3479
Size: 1210 4377
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5587
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4198 136
Size: 107 122 71 631 605 2526 1525
Fragments arranged by size:
2526 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5582 5582
Size: 5587
BlpI GC'TnA_GC
Cuts at: 3004 3004
Size: 5587
Bme1580I G_kGCm'C
Cuts at: 1790 3648 4804 1790
Size: 1858 1156 2573
Fragments arranged by size:
2573 1858 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5587
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3318 4276 442
Size: 416 53 83 399 24 1901 958 1753
Fragments arranged by size:
1901 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5587
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4247 1239
Size: 3008 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3712 4577 4875 3712
Size: 865 298 4424
Fragments arranged by size:
4424 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4250 882
Size: 617 2751 2219
Fragments arranged by size:
2751 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2561 3378 493
Size: 711 1357 817 2702
Fragments arranged by size:
2702 1357 817 711
BsaBI GATnn'nnATC
Cuts at: 5581 5581
Size: 5587
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3394 3776 4411 275
Size: 53 83 186 936 1861 382 635 1451
Fragments arranged by size:
1861 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4020 4418 4692 4839 5250 4020
Size: 398 274 147 411 4357
Fragments arranged by size:
4357 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5362
Fragments arranged by size:
5362 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2666 1186
Size: 1480 4107
BseYI C'CCAG_C
Cuts at: 4790 5286 4790
Size: 496 5091
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5014
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3037 3798 4826 1540
Size: 275 186 1036 761 1028 2301
Fragments arranged by size:
2301 1036 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3648 3733 4804 3648
Size: 85 1071 4431
Fragments arranged by size:
4431 1071 85
BsmI GAATG_Cn'
Cuts at: 2255 2398 3098 3191 2255
Size: 143 700 93 4651
Fragments arranged by size:
4651 700 143 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2906 3648 3733 4804 1391
Size: 399 1116 742 85 1071 2174
Fragments arranged by size:
2174 1116 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5250 5250
Size: 5587
BspHI T'CATG_A
Cuts at: 2268 2766 3476 5206 2268
Size: 498 710 1730 2649
Fragments arranged by size:
2649 1730 710 498
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2942 844
Size: 2098 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3474 1394
Size: 429 1651 3507
Fragments arranged by size:
3507 1651 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2127 4082 4256 65
Size: 1183 879 1955 174 1396
Fragments arranged by size:
1955 1396 1183 879 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4231 4418 1700
Size: 110 2421 187 2869
Fragments arranged by size:
2869 2421 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5587
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5587
BssSI C'ACGA_G
Cuts at: 3647 4659 3647
Size: 1012 4575
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5587
BstBI TT'CG_AA
Cuts at: 2875 4391 2875
Size: 1516 4071
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5587
BstYI r'GATC_y
Cuts at: 1972 2459 2917 3284 3669 3686 5138 5224 5236
Size: 487 458 367 385 17 1452 86 12
Cuts at: 5236 5582 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 487 458 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2047 513
Size: 552 982 4053
Fragments arranged by size:
4053 982 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4848
Fragments arranged by size:
4848 683 56
BtsI GCAGTG_nn'
Cuts at: 2726 3103 3897 3917 2726
Size: 377 794 20 4396
Fragments arranged by size:
4396 794 377 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5587
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2385 2420 529
Size: 35 1821 35 3696
Fragments arranged by size:
3696 1821 35 35
DraI TTT'AAA
Cuts at: 2891 2891
Size: 5587
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4594 809
Size: 507 3278 1802
Fragments arranged by size:
3278 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3034 3923 5552
Size: 54 1425 50 275 18 1204 889 1629
Cuts at: 5552 8
Size: 43
Fragments arranged by size:
1629 1425 1204 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3034 1537
Size: 275 1222 4090
Fragments arranged by size:
4090 1222 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3517 1353
Size: 2164 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4174 4548 4694 387
Size: 1064 138 2585 374 146 1280
Fragments arranged by size:
2585 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2888 3650 4247 5034 5302 1239
Size: 393 1256 762 597 787 268 1524
Fragments arranged by size:
1524 1256 787 762 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2591 2591
Size: 5587
EcoRI G'AATT_C
Cuts at: 2008 2299 2896 3274 2008
Size: 291 597 378 4321
Fragments arranged by size:
4321 597 378 291
EcoRV GAT'ATC
Cuts at: 2599 2599
Size: 5587
FspI TGC'GCA
Cuts at: 4093 4093
Size: 5587
HaeII r_GCGC'y
Cuts at: 1678 4734 1678
Size: 3056 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2287 2319 2365 2397 2659
Size: 32 135 32 715 32 46 32 262
Cuts at: 2659 2691 4228 4260 4302 4334 1373
Size: 32 1537 32 42 32 2626
Fragments arranged by size:
2626 1537 715 262 135 46 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2931 3177 669
Size: 988 281 993 246 3079
Fragments arranged by size:
3079 993 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5587
HpaI GTT'AAC
Cuts at: 3177 3177
Size: 5587
KpnI G_GTAC'C
Cuts at: 2912 2912
Size: 5587
MfeI C'AATT_G
Cuts at: 3186 3186
Size: 5587
MluI A'CGCG_T
Cuts at: 5463 5463
Size: 5587
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4701 4885 1305
Size: 3396 184 2007
Fragments arranged by size:
3396 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3765
Fragments arranged by size:
3765 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3545 3904 4063 518
Size: 648 2379 359 159 2042
Fragments arranged by size:
2379 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3003 3680 4828 5073 1606
Size: 1397 677 1148 245 2120
Fragments arranged by size:
2120 1397 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2047 513
Size: 552 982 4053
Fragments arranged by size:
4053 982 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5587
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5587
NotI GC'GGCC_GC
Cuts at: 3034 3034
Size: 5587
NspI r_CATG'y
Cuts at: 1132 1229 2528 2945 4490 1132
Size: 97 1299 417 1545 2229
Fragments arranged by size:
2229 1545 1299 417 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5587
PciI A'CATG_T
Cuts at: 2524 4486 2524
Size: 1962 3625
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2456 1108
Size: 1348 4239
PfoI T'CCnGG_A
Cuts at: 2244 2455 2631 2244
Size: 211 176 5200
Fragments arranged by size:
5200 211 176
PmeI GTTT'AAAC
Cuts at: 2891 2891
Size: 5587
PmlI CAC'GTG
Cuts at: 2561 2561
Size: 5587
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3656 3688 5169 5201 3656
Size: 32 1481 32 4042
Fragments arranged by size:
4042 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5587
PsiI TTA'TAA
Cuts at: 3157 3157
Size: 5587
PstI C_TGCA'G
Cuts at: 830 2939 830
Size: 2109 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5587
SalI G'TCGA_C
Cuts at: 1936 2929 1936
Size: 993 4594
SbfI CC_TGCA'GG
Cuts at: 2939 2939
Size: 5587
ScaI AGT'ACT
Cuts at: 3835 5547 3835
Size: 1712 3875
SfcI C'TryA_G
Cuts at: 826 1045 2935 4070 4751 4942 826
Size: 219 1890 1135 681 191 1471
Fragments arranged by size:
1890 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5587
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5587
SmaI CCC'GGG
Cuts at: 1872 2914 1872
Size: 1042 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3691 4592 4854 820
Size: 197 926 1748 901 262 1553
Fragments arranged by size:
1748 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3378 493
Size: 2885 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5587
SphI G_CATG'C
Cuts at: 1132 2945 1132
Size: 1813 3774
SspI AAT'ATT
Cuts at: 5 52 2081 3511 5
Size: 47 2029 1430 2081
Fragments arranged by size:
2081 2029 1430 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5587
StyI C'CwwG_G
Cuts at: 513 1065 2047 3026 513
Size: 552 982 979 3074
Fragments arranged by size:
3074 982 979 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3614 3631 3784 3969 4414 325
Size: 1255 2034 17 153 185 445 1498
Fragments arranged by size:
2034 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2563 3833 5545
Size: 274 80 33 51 1443 586 1270 1712
Cuts at: 5545 96
Size: 138
Fragments arranged by size:
1712 1443 1270 586 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5547 5579 5547
Size: 32 5555
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5587
XbaI T'CTAG_A
Cuts at: 2923 2923
Size: 5587
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5587
XmaI C'CCGG_G
Cuts at: 1870 2912 1870
Size: 1042 4545
XmnI GAAnn'nnTTC
Cuts at: 2340 3716 2340
Size: 1376 4211
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3395 276
Size: 53 83 186 936 1861 2468
Fragments arranged by size:
2468 1861 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoO109I FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI