Restriction map for
pFN21AE1783
(Circular) (Six-base) MAPSORT of: pFN21AE1783.seq Check: 6805 from: 1 to: 5572
pFN21AE1783 5572 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2403 2629 2403
Size: 226 5346
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3382 278
Size: 53 83 186 936 1846 2468
Fragments arranged by size:
2468 1846 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2715 2915 1937
Size: 778 200 4594
Fragments arranged by size:
4594 778 200
Acc65I G'GTAC_C
Cuts at: 2893 2893
Size: 5572
AclI AA'CG_TT
Cuts at: 1563 3699 4072 1563
Size: 2136 373 3063
Fragments arranged by size:
3063 2136 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3635 5019 5287 1632
Size: 2003 1384 268 1917
Fragments arranged by size:
2003 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5375
AflIII A'CryG_T
Cuts at: 1203 2493 4471 5448 1203
Size: 1290 1978 977 1327
Fragments arranged by size:
1978 1327 1290 977
AgeI A'CCGG_T
Cuts at: 4403 4403
Size: 5572
AhdI GACnn_n'nnGTC
Cuts at: 4301 4301
Size: 5572
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5572
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5572
AlwNI CAG_nnn'CTG
Cuts at: 2662 3315 4887 2662
Size: 653 1572 3347
Fragments arranged by size:
3347 1572 653
ApaLI G'TGCA_C
Cuts at: 3629 4785 3629
Size: 1156 4416
ApoI r'AATT_y
Cuts at: 1493 2881 3107 3259 5368 1493
Size: 1388 226 152 2109 1697
Fragments arranged by size:
2109 1697 1388 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4126 160
Size: 872 3094 1606
Fragments arranged by size:
3094 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5572
AvaI C'yCGr_G
Cuts at: 1870 1943 2222 2436 2897 1870
Size: 73 279 214 461 4545
Fragments arranged by size:
4545 461 279 214 73
BamHI G'GATC_C
Cuts at: 2902 3269 2902
Size: 367 5205
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2336 2893 4348 618
Size: 325 217 627 549 557 1455 1842
Fragments arranged by size:
1842 1455 627 557 549 325 217
BanII G_rGCy'C
Cuts at: 1391 2229 2891 1391
Size: 838 662 4072
Fragments arranged by size:
4072 838 662
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2530 928
Size: 971 631 3970
Fragments arranged by size:
3970 971 631
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3763 3797 1903
Size: 34 1826 34 3678
Fragments arranged by size:
3678 1826 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3464 4674 3464
Size: 1210 4362
BclI T'GATC_A
Cuts at: 1548 2031 1548
Size: 483 5089
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2166 2193 2505
Size: 107 122 71 631 605 494 27 312
Cuts at: 2505 2778 2838 4183 136
Size: 273 60 1345 1525
Fragments arranged by size:
1525 1345 631 605 494 312 273 122
107 71 60 27
BglII A'GATC_T
Cuts at: 5567 5567
Size: 5572
BlpI GC'TnA_GC
Cuts at: 2687 2989 2687
Size: 302 5270
Bme1580I G_kGCm'C
Cuts at: 1790 2107 2759 3633 4789 1790
Size: 317 652 874 1156 2573
Fragments arranged by size:
2573 1156 874 652 317
BmgBI CAC'GTC
Cuts at: 1318 2181 2494 1318
Size: 863 313 4396
Fragments arranged by size:
4396 863 313
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2072 3303 4261
Size: 416 53 83 399 24 655 1231 958
Cuts at: 4261 442
Size: 1753
Fragments arranged by size:
1753 1231 958 655 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5572
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2717 4232 1239
Size: 1478 1515 2579
Fragments arranged by size:
2579 1515 1478
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2576 3697 4562 4860 2576
Size: 1121 865 298 3288
Fragments arranged by size:
3288 1121 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4235 882
Size: 617 2736 2219
Fragments arranged by size:
2736 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2524 3363 493
Size: 711 1320 839 2702
Fragments arranged by size:
2702 1320 839 711
BsaBI GATnn'nnATC
Cuts at: 5566 5566
Size: 5572
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2532 2586 3379 3761
Size: 53 83 186 936 999 54 793 382
Cuts at: 3761 4396 275
Size: 635 1451
Fragments arranged by size:
1451 999 936 793 635 382 186 83
54 53
BsaWI w'CCGG_w
Cuts at: 4005 4403 4677 4824 5235 4005
Size: 398 274 147 411 4342
Fragments arranged by size:
4342 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5347
Fragments arranged by size:
5347 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2039 2456 1186
Size: 853 417 4302
Fragments arranged by size:
4302 853 417
BseYI C'CCAG_C
Cuts at: 2196 2469 4775 5271 2196
Size: 273 2306 496 2497
Fragments arranged by size:
2497 2306 496 273
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2114 2195 2381 1163
Size: 573 378 81 186 4354
Fragments arranged by size:
4354 573 378 186 81
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2158 2188 3022 3783 4811 1540
Size: 275 186 157 30 834 761 1028 2301
Fragments arranged by size:
2301 1028 834 761 275 186 157 30
BsiHKAI G_wGCw'C
Cuts at: 2229 2457 2693 3633 3718 4789 2229
Size: 228 236 940 85 1071 3012
Fragments arranged by size:
3012 1071 940 236 228 85
BsmI GAATG_Cn'
Cuts at: 3083 3176 3083
Size: 93 5479
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2107 2229 2457 2693 2759 2891 3633
Size: 399 317 122 228 236 66 132 742
Cuts at: 3633 3718 4789 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 317 236 228 132
122 85 66
BspEI T'CCGG_A
Cuts at: 5235 5235
Size: 5572
BspHI T'CATG_A
Cuts at: 3461 5191 3461
Size: 1730 3842
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2250 2403 2629 2927 844
Size: 1406 153 226 298 3489
Fragments arranged by size:
3489 1406 298 226 153
BsrBI CCG'CTC
Cuts at: 1394 1823 3459 1394
Size: 429 1636 3507
Fragments arranged by size:
3507 1636 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2082 2376 4067 4241 65
Size: 1183 834 294 1691 174 1396
Fragments arranged by size:
1691 1396 1183 834 294 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2339 2777 2837 4216 4403 1700
Size: 110 529 438 60 1379 187 2869
Fragments arranged by size:
2869 1379 529 438 187 110 60
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5572
BssHII G'CGCG_C
Cuts at: 1925 2669 1925
Size: 744 4828
BssSI C'ACGA_G
Cuts at: 3632 4644 3632
Size: 1012 4560
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5572
BstBI TT'CG_AA
Cuts at: 4376 4376
Size: 5572
BstEII G'GTnAC_C
Cuts at: 1184 2390 1184
Size: 1206 4366
BstXI CCAn_nnnn'nTGG
Cuts at: 2299 2559 2299
Size: 260 5312
BstYI r'GATC_y
Cuts at: 1972 2259 2902 3269 3654 3671 5123 5209 5221
Size: 287 643 367 385 17 1452 86 12
Cuts at: 5221 5567 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 643 385 367 346 287 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2068 2385 2568 2826 513
Size: 552 1003 317 183 258 3259
Fragments arranged by size:
3259 1003 552 317 258 183
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2263 487
Size: 683 56 1037 3796
Fragments arranged by size:
3796 1037 683 56
BtsI GCAGTG_nn'
Cuts at: 2091 2350 2509 3088 3882 3902 2091
Size: 259 159 579 794 20 3761
Fragments arranged by size:
3761 794 579 259 159 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5572
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5537
DraI TTT'AAA
Cuts at: 2876 2876
Size: 5572
DraIII CAC_nnn'GTG
Cuts at: 2404 2461 2404
Size: 57 5515
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4579 809
Size: 507 3263 1802
Fragments arranged by size:
3263 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 2155 2185
Size: 54 1425 50 275 18 219 106 30
Cuts at: 2185 2526 3019 3908 5537 8
Size: 341 493 889 1629 43
Fragments arranged by size:
1629 1425 889 493 341 275 219 106
54 50 43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 2155 2185 3019 1537
Size: 275 343 30 834 4090
Fragments arranged by size:
4090 834 343 275 30
EarI CTCTTCn'nnn_
Cuts at: 1353 3502 1353
Size: 2149 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4159 4533 4679 387
Size: 1064 138 2570 374 146 1280
Fragments arranged by size:
2570 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2227 2227
Size: 5572
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2717 3635 4232 5019 5287 1239
Size: 393 1085 918 597 787 268 1524
Fragments arranged by size:
1524 1085 918 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2881 3259 2881
Size: 378 5194
FspI TGC'GCA
Cuts at: 2483 2726 4078 2483
Size: 243 1352 3977
Fragments arranged by size:
3977 1352 243
FspAI rTGC'GCAy
Cuts at: 2483 2726 2483
Size: 243 5329
HaeII r_GCGC'y
Cuts at: 1678 2611 2663 4719 1678
Size: 933 52 2056 2531
Fragments arranged by size:
2531 2056 933 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2537 2569 4213 4245 4287
Size: 32 135 32 965 32 1644 32 42
Cuts at: 4287 4319 1373
Size: 32 2626
Fragments arranged by size:
2626 1644 965 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2146 2916 3162 669
Size: 988 281 208 770 246 3079
Fragments arranged by size:
3079 988 770 281 246 208
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5572
HpaI GTT'AAC
Cuts at: 3162 3162
Size: 5572
KpnI G_GTAC'C
Cuts at: 2897 2897
Size: 5572
MfeI C'AATT_G
Cuts at: 3171 3171
Size: 5572
MluI A'CGCG_T
Cuts at: 5448 5448
Size: 5572
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2280 2808 4686 4870 1305
Size: 975 528 1878 184 2007
Fragments arranged by size:
2007 1878 975 528 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3750
Fragments arranged by size:
3750 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2291 2294 2813 3530 3889 4048 518
Size: 648 1125 3 519 717 359 159 2042
Fragments arranged by size:
2042 1125 717 648 519 359 159 3
MspA1I CmG'CkG
Cuts at: 1606 2422 2469 2805 2988 3665 4813 5058 1606
Size: 816 47 336 183 677 1148 245 2120
Fragments arranged by size:
2120 1148 816 677 336 245 183 47
NaeI GCC'GGC
Cuts at: 2779 2779
Size: 5572
NcoI C'CATG_G
Cuts at: 513 1065 2068 513
Size: 552 1003 4017
Fragments arranged by size:
4017 1003 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5572
NgoMIV G'CCGG_C
Cuts at: 2777 2777
Size: 5572
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5572
NotI GC'GGCC_GC
Cuts at: 3019 3019
Size: 5572
NspI r_CATG'y
Cuts at: 1132 1229 2655 2930 4475 1132
Size: 97 1426 275 1545 2229
Fragments arranged by size:
2229 1545 1426 275 97
PasI CC'CwG_GG
Cuts at: 1348 2559 1348
Size: 1211 4361
PciI A'CATG_T
Cuts at: 4471 4471
Size: 5572
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2662 1108
Size: 1554 4018
PfoI T'CCnGG_A
Cuts at: 2448 2541 2448
Size: 93 5479
PmeI GTTT'AAAC
Cuts at: 2876 2876
Size: 5572
PmlI CAC'GTG
Cuts at: 2524 2524
Size: 5572
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3641 3673 5154 5186 3641
Size: 32 1481 32 4027
Fragments arranged by size:
4027 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5572
PsiI TTA'TAA
Cuts at: 3142 3142
Size: 5572
PstI C_TGCA'G
Cuts at: 830 2924 830
Size: 2094 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5572
SacI G_AGCT'C
Cuts at: 2229 2229
Size: 5572
SalI G'TCGA_C
Cuts at: 1936 2914 1936
Size: 978 4594
SbfI CC_TGCA'GG
Cuts at: 2924 2924
Size: 5572
ScaI AGT'ACT
Cuts at: 3820 5532 3820
Size: 1712 3860
SfcI C'TryA_G
Cuts at: 826 1045 2920 4055 4736 4927 826
Size: 219 1875 1135 681 191 1471
Fragments arranged by size:
1875 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5572
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5572
SmaI CCC'GGG
Cuts at: 1872 2438 2899 1872
Size: 566 461 4545
Fragments arranged by size:
4545 566 461
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2591 3676 4577 4839 820
Size: 197 926 648 1085 901 262 1553
Fragments arranged by size:
1553 1085 926 901 648 262 197
SnaBI TAC'GTA
Cuts at: 493 3363 493
Size: 2870 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5572
SphI G_CATG'C
Cuts at: 1132 2930 1132
Size: 1798 3774
SspI AAT'ATT
Cuts at: 5 52 3496 5
Size: 47 3444 2081
Fragments arranged by size:
3444 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5572
StyI C'CwwG_G
Cuts at: 513 1065 2068 2172 3011 513
Size: 552 1003 104 839 3074
Fragments arranged by size:
3074 1003 839 552 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2188 2398 2461 2501 3599 3616 3769
Size: 1255 608 210 63 40 1098 17 153
Cuts at: 3769 3954 4399 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1098 608 445 210 185 153
63 40 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3818 5530 96
Size: 274 80 33 51 1443 1841 1712 138
Fragments arranged by size:
1841 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5532 5564 5532
Size: 32 5540
Tth111I GACn'n_nGTC
Cuts at: 1316 2534 1316
Size: 1218 4354
XbaI T'CTAG_A
Cuts at: 2908 2908
Size: 5572
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2059 2120 2011
Size: 48 61 5463
Fragments arranged by size:
5463 61 48
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5572
XmaI C'CCGG_G
Cuts at: 1870 2436 2897 1870
Size: 566 461 4545
Fragments arranged by size:
4545 566 461
XmnI GAAnn'nnTTC
Cuts at: 3701 3701
Size: 5572
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3380 276
Size: 53 83 186 936 1846 2468
Fragments arranged by size:
2468 1846 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PstI PvuI SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI
EcoO109I EcoRV FalI FseI KasI NarI NruI NsiI
PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI