Restriction map for
pFN21AE1781
(Circular) (Six-base) MAPSORT of: pFN21AE1781.seq Check: 8986 from: 1 to: 5572
pFN21AE1781 5572 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3382 278
Size: 53 83 186 936 1846 2468
Fragments arranged by size:
2468 1846 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2915 1937
Size: 978 4594
Acc65I G'GTAC_C
Cuts at: 2274 2893 2274
Size: 619 4953
AclI AA'CG_TT
Cuts at: 1563 3699 4072 1563
Size: 2136 373 3063
Fragments arranged by size:
3063 2136 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3635 5019 5287 1632
Size: 2003 1384 268 1917
Fragments arranged by size:
2003 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5375
AflIII A'CryG_T
Cuts at: 1203 2262 4471 5448 1203
Size: 1059 2209 977 1327
Fragments arranged by size:
2209 1327 1059 977
AgeI A'CCGG_T
Cuts at: 4403 4403
Size: 5572
AhdI GACnn_n'nnGTC
Cuts at: 2705 4301 2705
Size: 1596 3976
AleI CACnn'nnGTG
Cuts at: 1166 2579 1166
Size: 1413 4159
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5572
AlwNI CAG_nnn'CTG
Cuts at: 2235 2535 2638 2651 3315 4887 2235
Size: 300 103 13 664 1572 2920
Fragments arranged by size:
2920 1572 664 300 103 13
ApaI G_GGCC'C
Cuts at: 2454 2454
Size: 5572
ApaLI G'TGCA_C
Cuts at: 3629 4785 3629
Size: 1156 4416
ApoI r'AATT_y
Cuts at: 1493 2881 3107 3259 5368 1493
Size: 1388 226 152 2109 1697
Fragments arranged by size:
2109 1697 1388 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4126 160
Size: 872 3094 1606
Fragments arranged by size:
3094 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5572
AvaI C'yCGr_G
Cuts at: 1870 1943 2897 1870
Size: 73 954 4545
Fragments arranged by size:
4545 954 73
BamHI G'GATC_C
Cuts at: 2902 3269 2902
Size: 367 5205
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2274 2573 2893 4348 618
Size: 325 217 627 487 299 320 1455 1842
Fragments arranged by size:
1842 1455 627 487 325 320 299 217
BanII G_rGCy'C
Cuts at: 1391 2454 2527 2891 1391
Size: 1063 73 364 4072
Fragments arranged by size:
4072 1063 364 73
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4601
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3763 3797 1903
Size: 34 1826 34 3678
Fragments arranged by size:
3678 1826 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2009 3464 4674 2009
Size: 1455 1210 2907
Fragments arranged by size:
2907 1455 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5572
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4183 136
Size: 107 122 71 631 605 2511 1525
Fragments arranged by size:
2511 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2142 5567 2142
Size: 3425 2147
BlpI GC'TnA_GC
Cuts at: 2989 2989
Size: 5572
Bme1580I G_kGCm'C
Cuts at: 1790 2454 3633 4789 1790
Size: 664 1179 1156 2573
Fragments arranged by size:
2573 1179 1156 664
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5572
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2294 3303 4261
Size: 416 53 83 399 24 877 1009 958
Cuts at: 4261 442
Size: 1753
Fragments arranged by size:
1753 1009 958 877 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5572
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2780 4232 1239
Size: 1541 1452 2579
Fragments arranged by size:
2579 1541 1452
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2064 3697 4562 4860 2064
Size: 1633 865 298 2776
Fragments arranged by size:
2776 1633 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4235 882
Size: 617 2736 2219
Fragments arranged by size:
2736 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3363 493
Size: 711 2159 2702
Fragments arranged by size:
2702 2159 711
BsaBI GATnn'nnATC
Cuts at: 5566 5566
Size: 5572
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3379 3761 4396 275
Size: 53 83 186 936 1846 382 635 1451
Fragments arranged by size:
1846 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2505 4005 4403 4677 4824 5235 2505
Size: 1500 398 274 147 411 2842
Fragments arranged by size:
2842 1500 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5347
Fragments arranged by size:
5347 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5572
BseYI C'CCAG_C
Cuts at: 2597 2638 4775 5271 2597
Size: 41 2137 496 2898
Fragments arranged by size:
2898 2137 496 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2790 1163
Size: 573 1054 3945
Fragments arranged by size:
3945 1054 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2506 3022 3783 4811 1540
Size: 275 186 505 516 761 1028 2301
Fragments arranged by size:
2301 1028 761 516 505 275 186
BsiHKAI G_wGCw'C
Cuts at: 3633 3718 4789 3633
Size: 85 1071 4416
Fragments arranged by size:
4416 1071 85
BsmI GAATG_Cn'
Cuts at: 2847 3083 3176 2847
Size: 236 93 5243
Fragments arranged by size:
5243 236 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2454 2527 2891 3633 3718 4789 1391
Size: 399 664 73 364 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 664 399 364 85 73
BspEI T'CCGG_A
Cuts at: 5235 5235
Size: 5572
BspHI T'CATG_A
Cuts at: 3461 5191 3461
Size: 1730 3842
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2244 2927 844
Size: 1400 683 3489
Fragments arranged by size:
3489 1400 683
BsrBI CCG'CTC
Cuts at: 1394 1823 3459 1394
Size: 429 1636 3507
Fragments arranged by size:
3507 1636 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4067 4241 65
Size: 1183 2819 174 1396
Fragments arranged by size:
2819 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4216 4403 1700
Size: 110 2406 187 2869
Fragments arranged by size:
2869 2406 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5572
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5572
BssSI C'ACGA_G
Cuts at: 3632 4644 3632
Size: 1012 4560
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5572
BstBI TT'CG_AA
Cuts at: 4376 4376
Size: 5572
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5572
BstXI CCAn_nnnn'nTGG
Cuts at: 2839 2839
Size: 5572
BstYI r'GATC_y
Cuts at: 1972 2142 2902 3269 3654 3671 5123 5209 5221
Size: 170 760 367 385 17 1452 86 12
Cuts at: 5221 5567 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 760 385 367 346 170 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4081
Fragments arranged by size:
4081 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4833
Fragments arranged by size:
4833 683 56
BtsI GCAGTG_nn'
Cuts at: 2040 3088 3882 3902 2040
Size: 1048 794 20 3710
Fragments arranged by size:
3710 1048 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5572
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5537
DraI TTT'AAA
Cuts at: 2876 2876
Size: 5572
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4579 809
Size: 507 3263 1802
Fragments arranged by size:
3263 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2200 2841 3019
Size: 54 1425 50 275 18 370 641 178
Cuts at: 3019 3908 5537 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 641 370 275 178 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3019 1537
Size: 275 1207 4090
Fragments arranged by size:
4090 1207 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3502 1353
Size: 2149 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4159 4533 4679 387
Size: 1064 138 2570 374 146 1280
Fragments arranged by size:
2570 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2780 3635 4232 5019 5287 1239
Size: 393 1148 855 597 787 268 1524
Fragments arranged by size:
1524 1148 855 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2445 2762 2445
Size: 317 5255
EcoO109I rG'GnC_Cy
Cuts at: 2232 2450 2594 2232
Size: 218 144 5210
Fragments arranged by size:
5210 218 144
EcoRI G'AATT_C
Cuts at: 2881 3259 2881
Size: 378 5194
EcoRV GAT'ATC
Cuts at: 2389 2389
Size: 5572
FspI TGC'GCA
Cuts at: 4078 4078
Size: 5572
HaeII r_GCGC'y
Cuts at: 1678 4719 1678
Size: 3041 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2691 2723 4213 4245 4287
Size: 32 135 32 1119 32 1490 32 42
Cuts at: 4287 4319 1373
Size: 32 2626
Fragments arranged by size:
2626 1490 1119 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2916 3162 669
Size: 988 281 978 246 3079
Fragments arranged by size:
3079 988 978 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5572
HpaI GTT'AAC
Cuts at: 3162 3162
Size: 5572
KpnI G_GTAC'C
Cuts at: 2278 2897 2278
Size: 619 4953
MfeI C'AATT_G
Cuts at: 3171 3171
Size: 5572
MluI A'CGCG_T
Cuts at: 5448 5448
Size: 5572
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2362 4686 4870 1305
Size: 1057 2324 184 2007
Fragments arranged by size:
2324 2007 1057 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2843 10
Size: 54 1425 343 1011 2739
Fragments arranged by size:
2739 1425 1011 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2265 2579 2837 3530 3889 4048 518
Size: 648 1099 314 258 693 359 159 2042
Fragments arranged by size:
2042 1099 693 648 359 314 258 159
MspA1I CmG'CkG
Cuts at: 1606 2792 2988 3665 4813 5058 1606
Size: 1186 196 677 1148 245 2120
Fragments arranged by size:
2120 1186 1148 677 245 196
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4081
Fragments arranged by size:
4081 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5572
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5572
NotI GC'GGCC_GC
Cuts at: 3019 3019
Size: 5572
NspI r_CATG'y
Cuts at: 1132 1229 2266 2930 4475 1132
Size: 97 1037 664 1545 2229
Fragments arranged by size:
2229 1545 1037 664 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5572
PciI A'CATG_T
Cuts at: 2262 4471 2262
Size: 2209 3363
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2320 1108
Size: 1212 4360
PmeI GTTT'AAAC
Cuts at: 2876 2876
Size: 5572
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3641 3673 5154 5186 3641
Size: 32 1481 32 4027
Fragments arranged by size:
4027 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5572
PsiI TTA'TAA
Cuts at: 2034 3142 2034
Size: 1108 4464
PspOMI G'GGCC_C
Cuts at: 2450 2450
Size: 5572
PstI C_TGCA'G
Cuts at: 830 2047 2634 2924 830
Size: 1217 587 290 3478
Fragments arranged by size:
3478 1217 587 290
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5572
PvuII CAG'CTG
Cuts at: 2792 2792
Size: 5572
SalI G'TCGA_C
Cuts at: 1936 2914 1936
Size: 978 4594
SbfI CC_TGCA'GG
Cuts at: 2924 2924
Size: 5572
ScaI AGT'ACT
Cuts at: 3820 5532 3820
Size: 1712 3860
SexAI A'CCwGG_T
Cuts at: 2419 2419
Size: 5572
SfcI C'TryA_G
Cuts at: 826 1045 2043 2564 2630 2920 4055 4736 4927
Size: 219 998 521 66 290 1135 681 191
Cuts at: 4927 826
Size: 1471
Fragments arranged by size:
1471 1135 998 681 521 290 219 191
66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5572
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5572
SmaI CCC'GGG
Cuts at: 1872 2899 1872
Size: 1027 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2079 3676 4577 4839 820
Size: 197 926 136 1597 901 262 1553
Fragments arranged by size:
1597 1553 926 901 262 197 136
SnaBI TAC'GTA
Cuts at: 493 3363 493
Size: 2870 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5572
SphI G_CATG'C
Cuts at: 1132 2930 1132
Size: 1798 3774
SspI AAT'ATT
Cuts at: 5 52 3496 5
Size: 47 3444 2081
Fragments arranged by size:
3444 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5572
StyI C'CwwG_G
Cuts at: 513 1065 2004 3011 513
Size: 552 939 1007 3074
Fragments arranged by size:
3074 1007 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2312 3599 3616 3769 3954 4399 325
Size: 1255 732 1287 17 153 185 445 1498
Fragments arranged by size:
1498 1287 1255 732 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2180 2663 3818
Size: 274 80 33 51 1443 203 483 1155
Cuts at: 3818 5530 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1155 483 274 203 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5532 5564 5532
Size: 32 5540
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5572
XbaI T'CTAG_A
Cuts at: 2908 2908
Size: 5572
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5572
XmaI C'CCGG_G
Cuts at: 1870 2897 1870
Size: 1027 4545
XmnI GAAnn'nnTTC
Cuts at: 2052 3701 2052
Size: 1649 3923
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3380 276
Size: 53 83 186 936 1846 2468
Fragments arranged by size:
2468 1846 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI XcmI