Restriction map for
pFN21AE1780
(Circular) (Six-base) MAPSORT of: pFN21AE1780.seq Check: 5996 from: 1 to: 5569
pFN21AE1780 5569 bp
With 182 enzymes: SgfI *
June 10, 2016 17:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2171 2171
Size: 5569
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3379 278
Size: 53 83 186 936 1843 2468
Fragments arranged by size:
2468 1843 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2912 1937
Size: 975 4594
Acc65I G'GTAC_C
Cuts at: 2205 2890 2205
Size: 685 4884
AclI AA'CG_TT
Cuts at: 1563 3696 4069 1563
Size: 2133 373 3063
Fragments arranged by size:
3063 2133 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2651 3632 5016 5284 1632
Size: 1019 981 1384 268 1917
Fragments arranged by size:
1917 1384 1019 981 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5372
AflIII A'CryG_T
Cuts at: 1203 4468 5445 1203
Size: 3265 977 1327
Fragments arranged by size:
3265 1327 977
AgeI A'CCGG_T
Cuts at: 4400 4400
Size: 5569
AhdI GACnn_n'nnGTC
Cuts at: 4298 4298
Size: 5569
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5569
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2564 1184
Size: 1380 4189
AlwNI CAG_nnn'CTG
Cuts at: 2097 2629 2833 3312 4884 2097
Size: 532 204 479 1572 2782
Fragments arranged by size:
2782 1572 532 479 204
ApaI G_GGCC'C
Cuts at: 2344 2344
Size: 5569
ApaLI G'TGCA_C
Cuts at: 3626 4782 3626
Size: 1156 4413
ApoI r'AATT_y
Cuts at: 1493 2878 3104 3256 5365 1493
Size: 1385 226 152 2109 1697
Fragments arranged by size:
2109 1697 1385 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4123 160
Size: 872 3091 1606
Fragments arranged by size:
3091 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5569
AvaI C'yCGr_G
Cuts at: 1870 1943 2252 2583 2894 1870
Size: 73 309 331 311 4545
Fragments arranged by size:
4545 331 311 309 73
BamHI G'GATC_C
Cuts at: 2899 3266 2899
Size: 367 5202
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2020 2205 2360 2777 2890
Size: 325 217 627 233 185 155 417 113
Cuts at: 2890 4345 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 417 325 233 217 185
155 113
BanII G_rGCy'C
Cuts at: 1391 2219 2344 2430 2715 2804 2888 1391
Size: 828 125 86 285 89 84 4072
Fragments arranged by size:
4072 828 285 125 89 86 84
BbeI G_GCGC'C
Cuts at: 2024 2024
Size: 5569
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2708 928
Size: 971 809 3789
Fragments arranged by size:
3789 971 809
BbvCI CC'TCA_GC
Cuts at: 2644 2644
Size: 5569
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3760 3794 1903
Size: 34 50 34 1739 34 3678
Fragments arranged by size:
3678 1739 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3461 4671 3461
Size: 1210 4359
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5569
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2271 2311 2396
Size: 107 122 71 631 605 599 40 85
Cuts at: 2396 2538 2836 4180 136
Size: 142 298 1344 1525
Fragments arranged by size:
1525 1344 631 605 599 298 142 122
107 85 71 40
BglII A'GATC_T
Cuts at: 5564 5564
Size: 5569
BlpI GC'TnA_GC
Cuts at: 2803 2986 2803
Size: 183 5386
Bme1580I G_kGCm'C
Cuts at: 1790 2344 2460 2724 2780 2827 3630 4786 1790
Size: 554 116 264 56 47 803 1156 2573
Fragments arranged by size:
2573 1156 803 554 264 116 56 47
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5569
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2063 3300 4258
Size: 416 53 83 399 24 646 1237 958
Cuts at: 4258 442
Size: 1753
Fragments arranged by size:
1753 1237 958 646 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5569
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2659 2659
Size: 5569
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4229 1239
Size: 2990 2579
Bpu10I CC'TnA_GC
Cuts at: 2429 2644 2733 2429
Size: 215 89 5265
Fragments arranged by size:
5265 215 89
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3694 4559 4857 3694
Size: 865 298 4406
Fragments arranged by size:
4406 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4232 882
Size: 617 2733 2219
Fragments arranged by size:
2733 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3360 493
Size: 711 2156 2702
Fragments arranged by size:
2702 2156 711
BsaBI GATnn'nnATC
Cuts at: 5563 5563
Size: 5569
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2021 3376 3758 4393
Size: 53 83 186 936 488 1355 382 635
Cuts at: 4393 275
Size: 1451
Fragments arranged by size:
1451 1355 936 635 488 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4002 4400 4674 4821 5232 4002
Size: 398 274 147 411 4339
Fragments arranged by size:
4339 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5344
Fragments arranged by size:
5344 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5569
BseYI C'CCAG_C
Cuts at: 2119 2219 2471 2536 2826 4772 5268 2119
Size: 100 252 65 290 1946 496 2420
Fragments arranged by size:
2420 1946 496 290 252 100 65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4996
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3019 3780 4808 1540
Size: 275 186 1018 761 1028 2301
Fragments arranged by size:
2301 1028 1018 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2430 3630 3715 4786 2430
Size: 1200 85 1071 3213
Fragments arranged by size:
3213 1200 1071 85
BsmI GAATG_Cn'
Cuts at: 3080 3173 3080
Size: 93 5476
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2219 2344 2430 2460 2715 2724 2780
Size: 399 429 125 86 30 255 9 56
Cuts at: 2780 2804 2827 2888 3630 3715 4786 1391
Size: 24 23 61 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 429 399 255 125 86
85 61 56 30 24 23 9
BspEI T'CCGG_A
Cuts at: 5232 5232
Size: 5569
BspHI T'CATG_A
Cuts at: 3458 5188 3458
Size: 1730 3839
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2171 2924 844
Size: 1327 753 3489
Fragments arranged by size:
3489 1327 753
BsrBI CCG'CTC
Cuts at: 1394 1823 3456 1394
Size: 429 1633 3507
Fragments arranged by size:
3507 1633 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2356 4064 4238 65
Size: 1183 1108 1708 174 1396
Fragments arranged by size:
1708 1396 1183 1108 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2492 2618 4213 4400 1700
Size: 110 682 126 1595 187 2869
Fragments arranged by size:
2869 1595 682 187 126 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5569
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5569
BssSI C'ACGA_G
Cuts at: 2129 2592 3629 4641 2129
Size: 463 1037 1012 3057
Fragments arranged by size:
3057 1037 1012 463
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5569
BstBI TT'CG_AA
Cuts at: 4373 4373
Size: 5569
BstEII G'GTnAC_C
Cuts at: 1184 2134 1184
Size: 950 4619
BstXI CCAn_nnnn'nTGG
Cuts at: 2037 2386 2037
Size: 349 5220
BstYI r'GATC_y
Cuts at: 1972 2010 2899 3266 3651 3668 5120 5206 5218
Size: 38 889 367 385 17 1452 86 12
Cuts at: 5218 5564 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 889 385 367 346 86 38
17 12
Bsu36I CC'TnA_GG
Cuts at: 2841 2841
Size: 5569
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5017
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4830
Fragments arranged by size:
4830 683 56
BtsI GCAGTG_nn'
Cuts at: 2851 3085 3879 3899 2851
Size: 234 794 20 4521
Fragments arranged by size:
4521 794 234 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5569
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5534
DraI TTT'AAA
Cuts at: 2873 2873
Size: 5569
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4576 809
Size: 507 3260 1802
Fragments arranged by size:
3260 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2027 2063 2241
Size: 54 1425 50 275 18 197 36 178
Cuts at: 2241 2272 2494 2568 2620 3016 3905 5534 8
Size: 31 222 74 52 396 889 1629 43
Fragments arranged by size:
1629 1425 889 396 275 222 197 178
74 54 52 50 43 36 31 18
EagI C'GGCC_G
Cuts at: 1537 1812 3016 1537
Size: 275 1204 4090
Fragments arranged by size:
4090 1204 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3499 1353
Size: 2146 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4156 4530 4676 387
Size: 1064 138 2567 374 146 1280
Fragments arranged by size:
2567 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2428 2428
Size: 5569
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2651 3632 4229 5016 5284 1239
Size: 393 1019 981 597 787 268 1524
Fragments arranged by size:
1524 1019 981 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2482 2633 2482
Size: 151 5418
EcoO109I rG'GnC_Cy
Cuts at: 2094 2340 2563 2638 2094
Size: 246 223 75 5025
Fragments arranged by size:
5025 246 223 75
EcoRI G'AATT_C
Cuts at: 2878 3256 2878
Size: 378 5191
FspI TGC'GCA
Cuts at: 4075 4075
Size: 5569
HaeII r_GCGC'y
Cuts at: 1678 2024 4716 1678
Size: 346 2692 2531
Fragments arranged by size:
2692 2531 346
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4210 4242 4284 4316 1373
Size: 32 135 32 2638 32 42 32 2626
Fragments arranged by size:
2638 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2913 3159 669
Size: 988 281 975 246 3079
Fragments arranged by size:
3079 988 975 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5569
HpaI GTT'AAC
Cuts at: 3159 3159
Size: 5569
KasI G'GCGC_C
Cuts at: 2020 2020
Size: 5569
KpnI G_GTAC'C
Cuts at: 2209 2894 2209
Size: 685 4884
MfeI C'AATT_G
Cuts at: 3168 3168
Size: 5569
MluI A'CGCG_T
Cuts at: 5445 5445
Size: 5569
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2199 4683 4867 1305
Size: 894 2484 184 2007
Fragments arranged by size:
2484 2007 894 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2029 10
Size: 54 1425 343 197 3550
Fragments arranged by size:
3550 1425 343 197 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2330 3527 3886 4045 518
Size: 648 1164 1197 359 159 2042
Fragments arranged by size:
2042 1197 1164 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2358 2807 2985 3662 4810 5055 1606
Size: 752 449 178 677 1148 245 2120
Fragments arranged by size:
2120 1148 752 677 449 245 178
NaeI GCC'GGC
Cuts at: 2494 2494
Size: 5569
NarI GG'CG_CC
Cuts at: 2021 2021
Size: 5569
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5017
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5569
NgoMIV G'CCGG_C
Cuts at: 2492 2492
Size: 5569
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5569
NotI GC'GGCC_GC
Cuts at: 3016 3016
Size: 5569
NspI r_CATG'y
Cuts at: 1132 1229 2927 4472 1132
Size: 97 1698 1545 2229
Fragments arranged by size:
2229 1698 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5569
PciI A'CATG_T
Cuts at: 4468 4468
Size: 5569
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2509 1108
Size: 1401 4168
PmeI GTTT'AAAC
Cuts at: 2873 2873
Size: 5569
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3638 3670 5151 5183 3638
Size: 32 1481 32 4024
Fragments arranged by size:
4024 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2563 2638 2563
Size: 75 5494
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5569
PsiI TTA'TAA
Cuts at: 3139 3139
Size: 5569
PspOMI G'GGCC_C
Cuts at: 2340 2340
Size: 5569
PstI C_TGCA'G
Cuts at: 830 2168 2921 830
Size: 1338 753 3478
Fragments arranged by size:
3478 1338 753
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5569
PvuII CAG'CTG
Cuts at: 2807 2807
Size: 5569
SacI G_AGCT'C
Cuts at: 2430 2430
Size: 5569
SalI G'TCGA_C
Cuts at: 1936 2911 1936
Size: 975 4594
SbfI CC_TGCA'GG
Cuts at: 2921 2921
Size: 5569
ScaI AGT'ACT
Cuts at: 3817 5529 3817
Size: 1712 3857
SfcI C'TryA_G
Cuts at: 826 1045 2164 2917 4052 4733 4924 826
Size: 219 1119 753 1135 681 191 1471
Fragments arranged by size:
1471 1135 1119 753 681 219 191
SfoI GGC'GCC
Cuts at: 2022 2022
Size: 5569
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5569
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5569
SmaI CCC'GGG
Cuts at: 1872 2585 2896 1872
Size: 713 311 4545
Fragments arranged by size:
4545 713 311
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3673 4574 4836 820
Size: 197 926 1730 901 262 1553
Fragments arranged by size:
1730 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3360 493
Size: 2867 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5569
SphI G_CATG'C
Cuts at: 1132 2927 1132
Size: 1795 3774
SspI AAT'ATT
Cuts at: 5 52 3493 5
Size: 47 3441 2081
Fragments arranged by size:
3441 2081 47
StuI AGG'CCT
Cuts at: 1517 2839 1517
Size: 1322 4247
StyI C'CwwG_G
Cuts at: 513 1065 2394 3008 513
Size: 552 1329 614 3074
Fragments arranged by size:
3074 1329 614 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2153 2497 3596 3613 3766 3951 4396
Size: 1255 573 344 1099 17 153 185 445
Cuts at: 4396 325
Size: 1498
Fragments arranged by size:
1498 1255 1099 573 445 344 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3815 5527 96
Size: 274 80 33 51 1443 1838 1712 138
Fragments arranged by size:
1838 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2675 2707 5529 5561 2675
Size: 32 2822 32 2683
Fragments arranged by size:
2822 2683 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5569
XbaI T'CTAG_A
Cuts at: 2905 2905
Size: 5569
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2022 2094 2022
Size: 72 5497
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5569
XmaI C'CCGG_G
Cuts at: 1870 2583 2894 1870
Size: 713 311 4545
Fragments arranged by size:
4545 713 311
XmnI GAAnn'nnTTC
Cuts at: 3698 3698
Size: 5569
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3377 276
Size: 53 83 186 936 1843 2468
Fragments arranged by size:
2468 1843 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SrfI SwaI