Restriction map for
pFN21AE1776
(Circular) (Six-base) MAPSORT of: pFN21AE1776.seq Check: 7145 from: 1 to: 5557
pFN21AE1776 5557 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3367 278
Size: 53 83 186 936 1831 2468
Fragments arranged by size:
2468 1831 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2399 2900 1937
Size: 462 501 4594
Fragments arranged by size:
4594 501 462
Acc65I G'GTAC_C
Cuts at: 2878 2878
Size: 5557
AclI AA'CG_TT
Cuts at: 1563 3684 4057 1563
Size: 2121 373 3063
Fragments arranged by size:
3063 2121 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2483 3620 5004 5272 1632
Size: 851 1137 1384 268 1917
Fragments arranged by size:
1917 1384 1137 851 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5360
AflIII A'CryG_T
Cuts at: 1203 2292 4456 5433 1203
Size: 1089 2164 977 1327
Fragments arranged by size:
2164 1327 1089 977
AgeI A'CCGG_T
Cuts at: 4388 4388
Size: 5557
AhdI GACnn_n'nnGTC
Cuts at: 4286 4286
Size: 5557
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5557
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5557
AlwNI CAG_nnn'CTG
Cuts at: 2408 3300 4872 2408
Size: 892 1572 3093
Fragments arranged by size:
3093 1572 892
ApaLI G'TGCA_C
Cuts at: 3614 4770 3614
Size: 1156 4401
ApoI r'AATT_y
Cuts at: 1493 2866 3092 3244 5353 1493
Size: 1373 226 152 2109 1697
Fragments arranged by size:
2109 1697 1373 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4111 160
Size: 872 3079 1606
Fragments arranged by size:
3079 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5557
AvaI C'yCGr_G
Cuts at: 1870 1943 2882 1870
Size: 73 939 4545
Fragments arranged by size:
4545 939 73
BamHI G'GATC_C
Cuts at: 2887 3254 2887
Size: 367 5190
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2075 2812 2878 4333 618
Size: 325 217 627 288 737 66 1455 1842
Fragments arranged by size:
1842 1455 737 627 325 288 217 66
BanII G_rGCy'C
Cuts at: 1391 2029 2039 2157 2876 1391
Size: 638 10 118 719 4072
Fragments arranged by size:
4072 719 638 118 10
BbeI G_GCGC'C
Cuts at: 2079 2079
Size: 5557
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2259 2500 928
Size: 971 360 241 3985
Fragments arranged by size:
3985 971 360 241
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3748 3782 1903
Size: 34 1811 34 3678
Fragments arranged by size:
3678 1811 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3449 4659 3449
Size: 1210 4347
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5557
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2121 4168 136
Size: 107 122 71 631 605 449 2047 1525
Fragments arranged by size:
2047 1525 631 605 449 122 107 71
BglII A'GATC_T
Cuts at: 5552 5552
Size: 5557
BlpI GC'TnA_GC
Cuts at: 2974 2974
Size: 5557
Bme1580I G_kGCm'C
Cuts at: 1790 2817 3618 4774 1790
Size: 1027 801 1156 2573
Fragments arranged by size:
2573 1156 1027 801
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5557
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3288 4246 442
Size: 416 53 83 399 24 1871 958 1753
Fragments arranged by size:
1871 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5557
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2414 2680 2816 4217 1239
Size: 1175 266 136 1401 2579
Fragments arranged by size:
2579 1401 1175 266 136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3682 4547 4845 3682
Size: 865 298 4394
Fragments arranged by size:
4394 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4220 882
Size: 617 2721 2219
Fragments arranged by size:
2721 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2146 2566 3348 493
Size: 711 942 420 782 2702
Fragments arranged by size:
2702 942 782 711 420
BsaBI GATnn'nnATC
Cuts at: 2204 5551 2204
Size: 3347 2210
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2076 2703 3364 3746
Size: 53 83 186 936 543 627 661 382
Cuts at: 3746 4381 275
Size: 635 1451
Fragments arranged by size:
1451 936 661 635 627 543 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3990 4388 4662 4809 5220 3990
Size: 398 274 147 411 4327
Fragments arranged by size:
4327 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2282 2312 1178
Size: 30 165 30 879 30 4423
Fragments arranged by size:
4423 879 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2801 1186
Size: 1615 3942
BseYI C'CCAG_C
Cuts at: 2071 4760 5256 2071
Size: 2689 496 2372
Fragments arranged by size:
2689 2372 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4984
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2186 2743 3007 3768 4796 1540
Size: 275 186 185 557 264 761 1028 2301
Fragments arranged by size:
2301 1028 761 557 275 264 186 185
BsiHKAI G_wGCw'C
Cuts at: 2088 2300 3618 3703 4774 2088
Size: 212 1318 85 1071 2871
Fragments arranged by size:
2871 1318 1071 212 85
BsmI GAATG_Cn'
Cuts at: 3068 3161 3068
Size: 93 5464
BsmBI CGTCTCn'nnnn_
Cuts at: 2054 2054
Size: 5557
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2029 2039 2088 2157 2300 2817 2876
Size: 399 239 10 49 69 143 517 59
Cuts at: 2876 3618 3703 4774 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 517 399 239 143 85
69 59 49 10
BspEI T'CCGG_A
Cuts at: 5220 5220
Size: 5557
BspHI T'CATG_A
Cuts at: 3446 5176 3446
Size: 1730 3827
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2912 844
Size: 2068 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3444 1394
Size: 429 1621 3507
Fragments arranged by size:
3507 1621 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4052 4226 65
Size: 1183 2804 174 1396
Fragments arranged by size:
2804 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2111 4201 4388 1700
Size: 110 301 2090 187 2869
Fragments arranged by size:
2869 2090 301 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5557
BssHII G'CGCG_C
Cuts at: 1925 2107 1925
Size: 182 5375
BssSI C'ACGA_G
Cuts at: 3617 4629 3617
Size: 1012 4545
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5557
BstBI TT'CG_AA
Cuts at: 4361 4361
Size: 5557
BstEII G'GTnAC_C
Cuts at: 1184 2602 1184
Size: 1418 4139
BstYI r'GATC_y
Cuts at: 1972 2887 3254 3639 3656 5108 5194 5206 5552
Size: 915 367 385 17 1452 86 12 346
Cuts at: 5552 1972
Size: 1977
Fragments arranged by size:
1977 1452 915 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2400 2400
Size: 5557
BtgI C'CryG_G
Cuts at: 513 1065 2004 2817 513
Size: 552 939 813 3253
Fragments arranged by size:
3253 939 813 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4818
Fragments arranged by size:
4818 683 56
BtsI GCAGTG_nn'
Cuts at: 3073 3867 3887 3073
Size: 794 20 4743
Fragments arranged by size:
4743 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5557
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5522
DraI TTT'AAA
Cuts at: 2861 2861
Size: 5557
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4564 809
Size: 507 3248 1802
Fragments arranged by size:
3248 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2148 2183 2453
Size: 54 1425 50 275 18 318 35 270
Cuts at: 2453 2532 3004 3893 5522 8
Size: 79 472 889 1629 43
Fragments arranged by size:
1629 1425 889 472 318 275 270 79
54 50 43 35 18
EagI C'GGCC_G
Cuts at: 1537 1812 2183 3004 1537
Size: 275 371 821 4090
Fragments arranged by size:
4090 821 371 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2801 3487 1353
Size: 1448 686 3423
Fragments arranged by size:
3423 1448 686
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2019 4144 4518 4664 387
Size: 1064 138 430 2125 374 146 1280
Fragments arranged by size:
2125 1280 1064 430 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2414 2483 2680 2816 3620 4217 5004
Size: 393 782 69 197 136 804 597 787
Cuts at: 5004 5272 1239
Size: 268 1524
Fragments arranged by size:
1524 804 787 782 597 393 268 197
136 69
EcoRI G'AATT_C
Cuts at: 2866 3244 2866
Size: 378 5179
FspI TGC'GCA
Cuts at: 4063 4063
Size: 5557
HaeII r_GCGC'y
Cuts at: 1678 2079 2141 4704 1678
Size: 401 62 2563 2531
Fragments arranged by size:
2563 2531 401 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4198 4230 4272 4304 1373
Size: 32 135 32 2626 32 42 32 2626
Fragments arranged by size:
2626 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2901 3147 669
Size: 988 281 963 246 3079
Fragments arranged by size:
3079 988 963 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5557
HpaI GTT'AAC
Cuts at: 3147 3147
Size: 5557
KasI G'GCGC_C
Cuts at: 2075 2075
Size: 5557
KpnI G_GTAC'C
Cuts at: 2882 2882
Size: 5557
MfeI C'AATT_G
Cuts at: 3156 3156
Size: 5557
MluI A'CGCG_T
Cuts at: 5433 5433
Size: 5557
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4671 4855 1305
Size: 3366 184 2007
Fragments arranged by size:
3366 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2455 10
Size: 54 1425 343 623 3112
Fragments arranged by size:
3112 1425 623 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3515 3874 4033 518
Size: 648 2349 359 159 2042
Fragments arranged by size:
2349 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2098 2546 2973 3650 4798 5043 1606
Size: 492 448 427 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 492 448 427 245
NaeI GCC'GGC
Cuts at: 2113 2113
Size: 5557
NarI GG'CG_CC
Cuts at: 2076 2076
Size: 5557
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4066
Fragments arranged by size:
4066 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5557
NgoMIV G'CCGG_C
Cuts at: 2111 2111
Size: 5557
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5557
NotI GC'GGCC_GC
Cuts at: 3004 3004
Size: 5557
NspI r_CATG'y
Cuts at: 1132 1229 2296 2915 4460 1132
Size: 97 1067 619 1545 2229
Fragments arranged by size:
2229 1545 1067 619 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5557
PciI A'CATG_T
Cuts at: 2292 4456 2292
Size: 2164 3393
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5557
PmeI GTTT'AAAC
Cuts at: 2861 2861
Size: 5557
PmlI CAC'GTG
Cuts at: 2566 2566
Size: 5557
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3626 3658 5139 5171 3626
Size: 32 1481 32 4012
Fragments arranged by size:
4012 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2339 1613
Size: 726 4831
PsiI TTA'TAA
Cuts at: 3127 3127
Size: 5557
PstI C_TGCA'G
Cuts at: 830 2121 2909 830
Size: 1291 788 3478
Fragments arranged by size:
3478 1291 788
PvuI CG_AT'CG
Cuts at: 2001 2743 2001
Size: 742 4815
PvuII CAG'CTG
Cuts at: 2546 2546
Size: 5557
SalI G'TCGA_C
Cuts at: 1936 2899 1936
Size: 963 4594
SbfI CC_TGCA'GG
Cuts at: 2121 2909 2121
Size: 788 4769
ScaI AGT'ACT
Cuts at: 3805 5517 3805
Size: 1712 3845
SexAI A'CCwGG_T
Cuts at: 2853 2853
Size: 5557
SfcI C'TryA_G
Cuts at: 826 1045 2117 2905 4040 4721 4912 826
Size: 219 1072 788 1135 681 191 1471
Fragments arranged by size:
1471 1135 1072 788 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2121 2121
Size: 5557
SfoI GGC'GCC
Cuts at: 2077 2077
Size: 5557
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5557
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5557
SmaI CCC'GGG
Cuts at: 1872 2884 1872
Size: 1012 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3661 4562 4824 820
Size: 197 926 1718 901 262 1553
Fragments arranged by size:
1718 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3348 493
Size: 2855 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5557
SphI G_CATG'C
Cuts at: 1132 2915 1132
Size: 1783 3774
SspI AAT'ATT
Cuts at: 5 52 3481 5
Size: 47 3429 2081
Fragments arranged by size:
3429 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5557
StyI C'CwwG_G
Cuts at: 513 1065 2004 2364 2996 513
Size: 552 939 360 632 3074
Fragments arranged by size:
3074 939 632 552 360
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3584 3601 3754 3939 4384 325
Size: 1255 2004 17 153 185 445 1498
Fragments arranged by size:
2004 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3803 5515 96
Size: 274 80 33 51 1443 1826 1712 138
Fragments arranged by size:
1826 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2661 2693 5517 5549 2661
Size: 32 2824 32 2669
Fragments arranged by size:
2824 2669 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5557
XbaI T'CTAG_A
Cuts at: 2893 2893
Size: 5557
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2068 2068
Size: 5557
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5557
XmaI C'CCGG_G
Cuts at: 1870 2882 1870
Size: 1012 4545
XmnI GAAnn'nnTTC
Cuts at: 3686 3686
Size: 5557
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3365 276
Size: 53 83 186 936 1831 2468
Fragments arranged by size:
2468 1831 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI
PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SrfI SwaI