Restriction map for
pFN21AE1774
(Circular) (Six-base) MAPSORT of: pFN21AE1774.seq Check: 1138 from: 1 to: 5548
pFN21AE1774 5548 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3358 278
Size: 53 83 186 936 1822 2468
Fragments arranged by size:
2468 1822 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2571 2891 1937
Size: 634 320 4594
Fragments arranged by size:
4594 634 320
Acc65I G'GTAC_C
Cuts at: 2869 2869
Size: 5548
AclI AA'CG_TT
Cuts at: 1563 3675 4048 1563
Size: 2112 373 3063
Fragments arranged by size:
3063 2112 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3611 4995 5263 1632
Size: 1979 1384 268 1917
Fragments arranged by size:
1979 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5351
AflIII A'CryG_T
Cuts at: 1203 4447 5424 1203
Size: 3244 977 1327
Fragments arranged by size:
3244 1327 977
AgeI A'CCGG_T
Cuts at: 4379 4379
Size: 5548
AhdI GACnn_n'nnGTC
Cuts at: 4277 4277
Size: 5548
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5548
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5548
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2675 2707 2675
Size: 32 5516
AlwNI CAG_nnn'CTG
Cuts at: 3291 4863 3291
Size: 1572 3976
ApaLI G'TGCA_C
Cuts at: 3605 4761 3605
Size: 1156 4392
ApoI r'AATT_y
Cuts at: 1493 2600 2857 3083 3235 5344 1493
Size: 1107 257 226 152 2109 1697
Fragments arranged by size:
2109 1697 1107 257 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4102 160
Size: 872 3070 1606
Fragments arranged by size:
3070 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5548
AvaI C'yCGr_G
Cuts at: 1870 1943 2475 2873 1870
Size: 73 532 398 4545
Fragments arranged by size:
4545 532 398 73
BamHI G'GATC_C
Cuts at: 2471 2878 3245 2471
Size: 407 367 4774
Fragments arranged by size:
4774 407 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2784 2869 4324 618
Size: 325 217 627 997 85 1455 1842
Fragments arranged by size:
1842 1455 997 627 325 217 85
BanII G_rGCy'C
Cuts at: 1391 2867 1391
Size: 1476 4072
BbeI G_GCGC'C
Cuts at: 2788 2788
Size: 5548
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4577
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3739 3773 1903
Size: 34 1802 34 3678
Fragments arranged by size:
3678 1802 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3440 4650 3440
Size: 1210 4338
BclI T'GATC_A
Cuts at: 1548 2523 1548
Size: 975 4573
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2543 4159 136
Size: 107 122 71 631 605 871 1616 1525
Fragments arranged by size:
1616 1525 871 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5543 5543
Size: 5548
BlpI GC'TnA_GC
Cuts at: 2965 2965
Size: 5548
Bme1580I G_kGCm'C
Cuts at: 1790 2232 3609 4765 1790
Size: 442 1377 1156 2573
Fragments arranged by size:
2573 1377 1156 442
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5548
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2205 2809 3279
Size: 416 53 83 399 24 788 604 470
Cuts at: 3279 4237 442
Size: 958 1753
Fragments arranged by size:
1753 958 788 604 470 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5548
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2783 4208 1239
Size: 1544 1425 2579
Fragments arranged by size:
2579 1544 1425
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3673 4538 4836 3673
Size: 865 298 4385
Fragments arranged by size:
4385 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2292 4211 882
Size: 617 793 1919 2219
Fragments arranged by size:
2219 1919 793 617
BsaAI yAC'GTr
Cuts at: 493 1204 3339 493
Size: 711 2135 2702
Fragments arranged by size:
2702 2135 711
BsaBI GATnn'nnATC
Cuts at: 2105 5542 2105
Size: 3437 2111
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2023 2785 3355
Size: 53 83 186 936 476 14 762 570
Cuts at: 3355 3737 4372 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 762 635 570 476 382 186
83 53 14
BsaWI w'CCGG_w
Cuts at: 2026 2555 3981 4379 4653 4800 5211 2026
Size: 529 1426 398 274 147 411 2363
Fragments arranged by size:
2363 1426 529 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2675 2705 1178
Size: 30 165 30 1272 30 4021
Fragments arranged by size:
4021 1272 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5548
BseYI C'CCAG_C
Cuts at: 2123 2308 4751 5247 2123
Size: 185 2443 496 2424
Fragments arranged by size:
2443 2424 496 185
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2399 1163
Size: 573 663 4312
Fragments arranged by size:
4312 663 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2020 2827 2998 3759 4787 1540
Size: 275 186 19 807 171 761 1028 2301
Fragments arranged by size:
2301 1028 807 761 275 186 171 19
BsiHKAI G_wGCw'C
Cuts at: 3609 3694 4765 3609
Size: 85 1071 4392
Fragments arranged by size:
4392 1071 85
BsmI GAATG_Cn'
Cuts at: 3059 3152 3059
Size: 93 5455
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2232 2867 3609 3694 4765 1391
Size: 399 442 635 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 635 442 399 85
BspEI T'CCGG_A
Cuts at: 2026 5211 2026
Size: 3185 2363
BspHI T'CATG_A
Cuts at: 3437 5167 3437
Size: 1730 3818
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2181 2310 2903 844
Size: 1337 129 593 3489
Fragments arranged by size:
3489 1337 593 129
BsrBI CCG'CTC
Cuts at: 1394 1823 2776 3435 1394
Size: 429 953 659 3507
Fragments arranged by size:
3507 953 659 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4043 4217 65
Size: 1183 2795 174 1396
Fragments arranged by size:
2795 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2019 2822 4192 4379 1700
Size: 110 209 803 1370 187 2869
Fragments arranged by size:
2869 1370 803 209 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5548
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5548
BssSI C'ACGA_G
Cuts at: 3608 4620 3608
Size: 1012 4536
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5548
BstBI TT'CG_AA
Cuts at: 4352 4352
Size: 5548
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5548
BstYI r'GATC_y
Cuts at: 1972 2471 2878 3245 3630 3647 5099 5185 5197
Size: 499 407 367 385 17 1452 86 12
Cuts at: 5197 5543 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 499 407 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2014 513
Size: 552 939 10 4047
Fragments arranged by size:
4047 939 552 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4809
Fragments arranged by size:
4809 683 56
BtsI GCAGTG_nn'
Cuts at: 2387 3064 3858 3878 2387
Size: 677 794 20 4057
Fragments arranged by size:
4057 794 677 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5548
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5513
DraI TTT'AAA
Cuts at: 2852 2852
Size: 5548
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4555 809
Size: 507 3239 1802
Fragments arranged by size:
3239 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2017 2824 2995
Size: 54 1425 50 275 18 187 807 171
Cuts at: 2995 3884 5513 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 807 275 187 171 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2017 2824 2995 1537
Size: 275 205 807 171 4090
Fragments arranged by size:
4090 807 275 205 171
EarI CTCTTCn'nnn_
Cuts at: 1353 2738 3478 1353
Size: 1385 740 3423
Fragments arranged by size:
3423 1385 740
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2052 4135 4509 4655 387
Size: 1064 138 463 2083 374 146 1280
Fragments arranged by size:
2083 1280 1064 463 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2783 3611 4208 4995 5263 1239
Size: 393 1151 828 597 787 268 1524
Fragments arranged by size:
1524 1151 828 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2857 3235 2857
Size: 378 5170
EcoRV GAT'ATC
Cuts at: 2107 2251 2107
Size: 144 5404
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2566 2860 2566
Size: 294 5254
FspI TGC'GCA
Cuts at: 4054 4054
Size: 5548
HaeII r_GCGC'y
Cuts at: 1678 2428 2788 4695 1678
Size: 750 360 1907 2531
Fragments arranged by size:
2531 1907 750 360
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2644 2676 2731 2763 4189
Size: 32 135 32 1072 32 55 32 1426
Cuts at: 4189 4221 4263 4295 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1426 1072 135 55 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2892 3138 669
Size: 988 281 954 246 3079
Fragments arranged by size:
3079 988 954 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5548
HpaI GTT'AAC
Cuts at: 3138 3138
Size: 5548
KasI G'GCGC_C
Cuts at: 2784 2784
Size: 5548
KpnI G_GTAC'C
Cuts at: 2873 2873
Size: 5548
MfeI C'AATT_G
Cuts at: 3147 3147
Size: 5548
MluI A'CGCG_T
Cuts at: 5424 5424
Size: 5548
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2496 4662 4846 1305
Size: 1191 2166 184 2007
Fragments arranged by size:
2166 2007 1191 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3726
Fragments arranged by size:
3726 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3506 3865 4024 518
Size: 648 2340 359 159 2042
Fragments arranged by size:
2340 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2016 2491 2964 3641 4789 5034 1606
Size: 410 475 473 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 475 473 410 245
NaeI GCC'GGC
Cuts at: 2021 2021
Size: 5548
NarI GG'CG_CC
Cuts at: 2785 2785
Size: 5548
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4057
Fragments arranged by size:
4057 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5548
NgoMIV G'CCGG_C
Cuts at: 2019 2019
Size: 5548
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5548
NotI GC'GGCC_GC
Cuts at: 2995 2995
Size: 5548
NspI r_CATG'y
Cuts at: 1132 1229 2906 4451 1132
Size: 97 1677 1545 2229
Fragments arranged by size:
2229 1677 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2679 1348
Size: 1331 4217
PciI A'CATG_T
Cuts at: 4447 4447
Size: 5548
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5548
PmeI GTTT'AAAC
Cuts at: 2852 2852
Size: 5548
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2675 2707 3617 3649 5130 5162 2675
Size: 32 910 32 1481 32 3061
Fragments arranged by size:
3061 1481 910 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5548
PsiI TTA'TAA
Cuts at: 3118 3118
Size: 5548
PstI C_TGCA'G
Cuts at: 830 2490 2900 830
Size: 1660 410 3478
Fragments arranged by size:
3478 1660 410
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5548
PvuII CAG'CTG
Cuts at: 2491 2491
Size: 5548
SacII CC_GC'GG
Cuts at: 2017 2017
Size: 5548
SalI G'TCGA_C
Cuts at: 1936 2890 1936
Size: 954 4594
SbfI CC_TGCA'GG
Cuts at: 2900 2900
Size: 5548
ScaI AGT'ACT
Cuts at: 3796 5508 3796
Size: 1712 3836
SfcI C'TryA_G
Cuts at: 826 1045 2486 2896 4031 4712 4903 826
Size: 219 1441 410 1135 681 191 1471
Fragments arranged by size:
1471 1441 1135 681 410 219 191
SfoI GGC'GCC
Cuts at: 2786 2786
Size: 5548
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5548
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5548
SmaI CCC'GGG
Cuts at: 1872 2875 1872
Size: 1003 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3652 4553 4815 820
Size: 197 926 1709 901 262 1553
Fragments arranged by size:
1709 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3339 493
Size: 2846 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5548
SphI G_CATG'C
Cuts at: 1132 2906 1132
Size: 1774 3774
SspI AAT'ATT
Cuts at: 5 52 3472 5
Size: 47 3420 2081
Fragments arranged by size:
3420 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5548
StyI C'CwwG_G
Cuts at: 513 1065 2004 2987 513
Size: 552 939 983 3074
Fragments arranged by size:
3074 983 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2227 2298 2746 2828 3575 3592 3745
Size: 1255 647 71 448 82 747 17 153
Cuts at: 3745 3930 4375 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 747 647 448 445 185 153
82 71 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2430 2817 3794
Size: 274 80 33 51 1443 453 387 977
Cuts at: 3794 5506 96
Size: 1712 138
Fragments arranged by size:
1712 1443 977 453 387 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5508 5540 5508
Size: 32 5516
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5548
XbaI T'CTAG_A
Cuts at: 2884 2884
Size: 5548
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5548
XmaI C'CCGG_G
Cuts at: 1870 2873 1870
Size: 1003 4545
XmnI GAAnn'nnTTC
Cuts at: 3677 3677
Size: 5548
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3356 276
Size: 53 83 186 936 1822 2468
Fragments arranged by size:
2468 1822 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I FseI FspAI NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SanDI SapI SexAI SfiI SrfI SwaI XcmI