Restriction map for
pFN21AE1771
(Circular) (Six-base) MAPSORT of: pFN21AE1771.seq Check: 7259 from: 1 to: 5536
pFN21AE1771 5536 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3346 278
Size: 53 83 186 936 1810 2468
Fragments arranged by size:
2468 1810 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2879 1937
Size: 942 4594
Acc65I G'GTAC_C
Cuts at: 2857 2857
Size: 5536
AclI AA'CG_TT
Cuts at: 1563 3663 4036 1563
Size: 2100 373 3063
Fragments arranged by size:
3063 2100 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2039 2210 2243 3599 4983 5251 1632
Size: 407 171 33 1356 1384 268 1917
Fragments arranged by size:
1917 1384 1356 407 268 171 33
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5339
AflIII A'CryG_T
Cuts at: 1203 4435 5412 1203
Size: 3232 977 1327
Fragments arranged by size:
3232 1327 977
AgeI A'CCGG_T
Cuts at: 4367 4367
Size: 5536
AhdI GACnn_n'nnGTC
Cuts at: 4265 4265
Size: 5536
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5536
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2768 1184
Size: 1584 3952
AlwNI CAG_nnn'CTG
Cuts at: 3279 4851 3279
Size: 1572 3964
ApaLI G'TGCA_C
Cuts at: 2415 3593 4749 2415
Size: 1178 1156 3202
Fragments arranged by size:
3202 1178 1156
ApoI r'AATT_y
Cuts at: 1493 2194 2374 2845 3071 3223 5332 1493
Size: 701 180 471 226 152 2109 1697
Fragments arranged by size:
2109 1697 701 471 226 180 152
AseI AT'TA_AT
Cuts at: 160 1032 4090 160
Size: 872 3058 1606
Fragments arranged by size:
3058 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5536
AvaI C'yCGr_G
Cuts at: 1870 1943 2057 2861 1870
Size: 73 114 804 4545
Fragments arranged by size:
4545 804 114 73
BamHI G'GATC_C
Cuts at: 2866 3233 2866
Size: 367 5169
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2167 2857 4312 618
Size: 325 217 627 380 690 1455 1842
Fragments arranged by size:
1842 1455 690 627 380 325 217
BanII G_rGCy'C
Cuts at: 1391 2559 2855 1391
Size: 1168 296 4072
Fragments arranged by size:
4072 1168 296
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4565
BbvCI CC'TCA_GC
Cuts at: 2144 2144
Size: 5536
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3727 3761 1903
Size: 34 1790 34 3678
Fragments arranged by size:
3678 1790 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3428 4638 3428
Size: 1210 4326
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5536
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4147 136
Size: 107 122 71 631 605 2475 1525
Fragments arranged by size:
2475 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5531 5531
Size: 5536
BlpI GC'TnA_GC
Cuts at: 2953 2953
Size: 5536
Bme1580I G_kGCm'C
Cuts at: 1790 2419 3597 4753 1790
Size: 629 1178 1156 2573
Fragments arranged by size:
2573 1178 1156 629
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5536
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3267 4225 442
Size: 416 53 83 399 24 1850 958 1753
Fragments arranged by size:
1850 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5536
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4196 1239
Size: 2957 2579
Bpu10I CC'TnA_GC
Cuts at: 2144 2144
Size: 5536
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2357 2386 3661 4526 4824 2357
Size: 29 1275 865 298 3069
Fragments arranged by size:
3069 1275 865 298 29
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4199 882
Size: 617 2700 2219
Fragments arranged by size:
2700 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3327 493
Size: 711 2123 2702
Fragments arranged by size:
2702 2123 711
BsaBI GATnn'nnATC
Cuts at: 5530 5530
Size: 5536
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3343 3725 4360 275
Size: 53 83 186 936 1810 382 635 1451
Fragments arranged by size:
1810 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3969 4367 4641 4788 5199 3969
Size: 398 274 147 411 4306
Fragments arranged by size:
4306 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5311
Fragments arranged by size:
5311 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2048 2246 1186
Size: 862 198 4476
Fragments arranged by size:
4476 862 198
BseYI C'CCAG_C
Cuts at: 2544 4739 5235 2544
Size: 2195 496 2845
Fragments arranged by size:
2845 2195 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4963
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2986 3747 4775 1540
Size: 275 186 985 761 1028 2301
Fragments arranged by size:
2301 1028 985 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2208 2419 2559 3597 3682 4753 2208
Size: 211 140 1038 85 1071 2991
Fragments arranged by size:
2991 1071 1038 211 140 85
BsmI GAATG_Cn'
Cuts at: 3047 3140 3047
Size: 93 5443
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2208 2419 2559 2855 3597 3682 4753
Size: 399 418 211 140 296 742 85 1071
Cuts at: 4753 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 418 399 296 211 140
85
BspEI T'CCGG_A
Cuts at: 5199 5199
Size: 5536
BspHI T'CATG_A
Cuts at: 3425 5155 3425
Size: 1730 3806
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2620 2891 844
Size: 1776 271 3489
Fragments arranged by size:
3489 1776 271
BsrBI CCG'CTC
Cuts at: 1394 1823 3423 1394
Size: 429 1600 3507
Fragments arranged by size:
3507 1600 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2667 4031 4205 65
Size: 1183 1419 1364 174 1396
Fragments arranged by size:
1419 1396 1364 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4180 4367 1700
Size: 110 2370 187 2869
Fragments arranged by size:
2869 2370 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5536
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5536
BssSI C'ACGA_G
Cuts at: 2310 3596 4608 2310
Size: 1286 1012 3238
Fragments arranged by size:
3238 1286 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5536
BstBI TT'CG_AA
Cuts at: 4340 4340
Size: 5536
BstEII G'GTnAC_C
Cuts at: 1184 2659 1184
Size: 1475 4061
BstXI CCAn_nnnn'nTGG
Cuts at: 2095 2095
Size: 5536
BstYI r'GATC_y
Cuts at: 1972 2866 3233 3618 3635 5087 5173 5185 5531
Size: 894 367 385 17 1452 86 12 346
Cuts at: 5531 1972
Size: 1977
Fragments arranged by size:
1977 1452 894 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2495 2523 2495
Size: 28 5508
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4984
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2164 487
Size: 683 56 938 3859
Fragments arranged by size:
3859 938 683 56
BtsI GCAGTG_nn'
Cuts at: 3052 3846 3866 3052
Size: 794 20 4722
Fragments arranged by size:
4722 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5536
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5501
DraI TTT'AAA
Cuts at: 2840 2840
Size: 5536
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4543 809
Size: 507 3227 1802
Fragments arranged by size:
3227 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2620 2983 3872
Size: 54 1425 50 275 18 790 363 889
Cuts at: 3872 5501 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 790 363 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2983 1537
Size: 275 1171 4090
Fragments arranged by size:
4090 1171 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2385 3466 1353
Size: 1032 1081 3423
Fragments arranged by size:
3423 1081 1032
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4123 4497 4643 387
Size: 1064 138 2534 374 146 1280
Fragments arranged by size:
2534 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2557 2557
Size: 5536
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2210 2243 3599 4196 4983 5251
Size: 393 407 171 33 1356 597 787 268
Cuts at: 5251 1239
Size: 1524
Fragments arranged by size:
1524 1356 787 597 407 393 268 171
33
EcoRI G'AATT_C
Cuts at: 2845 3223 2845
Size: 378 5158
FspI TGC'GCA
Cuts at: 4042 4042
Size: 5536
HaeII r_GCGC'y
Cuts at: 1678 4683 1678
Size: 3005 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2498 2530 4177 4209 4251
Size: 32 135 32 926 32 1647 32 42
Cuts at: 4251 4283 1373
Size: 32 2626
Fragments arranged by size:
2626 1647 926 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2104 2880 3126 669
Size: 988 281 166 776 246 3079
Fragments arranged by size:
3079 988 776 281 246 166
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5536
HpaI GTT'AAC
Cuts at: 3126 3126
Size: 5536
KpnI G_GTAC'C
Cuts at: 2861 2861
Size: 5536
MfeI C'AATT_G
Cuts at: 3135 3135
Size: 5536
MluI A'CGCG_T
Cuts at: 5412 5412
Size: 5536
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2112 4650 4834 1305
Size: 807 2538 184 2007
Fragments arranged by size:
2538 2007 807 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2622 10
Size: 54 1425 343 790 2924
Fragments arranged by size:
2924 1425 790 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3494 3853 4012 518
Size: 648 2328 359 159 2042
Fragments arranged by size:
2328 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2952 3629 4777 5022 1606
Size: 1346 677 1148 245 2120
Fragments arranged by size:
2120 1346 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4984
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5536
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5536
NotI GC'GGCC_GC
Cuts at: 2983 2983
Size: 5536
NspI r_CATG'y
Cuts at: 1132 1229 2095 2755 2894 4439 1132
Size: 97 866 660 139 1545 2229
Fragments arranged by size:
2229 1545 866 660 139 97
PasI CC'CwG_GG
Cuts at: 1348 2655 1348
Size: 1307 4229
PciI A'CATG_T
Cuts at: 4435 4435
Size: 5536
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2462 1108
Size: 1354 4182
PfoI T'CCnGG_A
Cuts at: 2383 2383
Size: 5536
PmeI GTTT'AAAC
Cuts at: 2840 2840
Size: 5536
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3605 3637 5118 5150 3605
Size: 32 1481 32 3991
Fragments arranged by size:
3991 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5536
PsiI TTA'TAA
Cuts at: 3106 3106
Size: 5536
PstI C_TGCA'G
Cuts at: 830 2888 830
Size: 2058 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5536
SacI G_AGCT'C
Cuts at: 2559 2559
Size: 5536
SalI G'TCGA_C
Cuts at: 1936 2878 1936
Size: 942 4594
SbfI CC_TGCA'GG
Cuts at: 2888 2888
Size: 5536
ScaI AGT'ACT
Cuts at: 3784 5496 3784
Size: 1712 3824
SfcI C'TryA_G
Cuts at: 826 1045 2884 4019 4700 4891 826
Size: 219 1839 1135 681 191 1471
Fragments arranged by size:
1839 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5536
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5536
SmaI CCC'GGG
Cuts at: 1872 2863 1872
Size: 991 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2336 2365 3640 4541 4803 820
Size: 197 926 393 29 1275 901 262 1553
Fragments arranged by size:
1553 1275 926 901 393 262 197 29
SnaBI TAC'GTA
Cuts at: 493 3327 493
Size: 2834 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5536
SphI G_CATG'C
Cuts at: 1132 2894 1132
Size: 1762 3774
SspI AAT'ATT
Cuts at: 5 52 3460 5
Size: 47 3408 2081
Fragments arranged by size:
3408 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5536
StyI C'CwwG_G
Cuts at: 513 1065 2281 2975 513
Size: 552 1216 694 3074
Fragments arranged by size:
3074 1216 694 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2647 3563 3580 3733 3918 4363 325
Size: 1255 1067 916 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1067 916 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3782 5494 96
Size: 274 80 33 51 1443 1805 1712 138
Fragments arranged by size:
1805 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2124 2156 5496 5528 2124
Size: 32 3340 32 2132
Fragments arranged by size:
3340 2132 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5536
XbaI T'CTAG_A
Cuts at: 2872 2872
Size: 5536
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2684 2684
Size: 5536
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5536
XmaI C'CCGG_G
Cuts at: 1870 2861 1870
Size: 991 4545
XmnI GAAnn'nnTTC
Cuts at: 3665 3665
Size: 5536
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3344 276
Size: 53 83 186 936 1810 2468
Fragments arranged by size:
2468 1810 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI