Restriction map for
pFN21AE1770
(Circular) (Six-base) MAPSORT of: pFN21AE1770.seq Check: 1257 from: 1 to: 5536
pFN21AE1770 5536 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2135 2290 2135
Size: 155 5381
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3346 278
Size: 53 83 186 936 1810 2468
Fragments arranged by size:
2468 1810 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2580 2745 2879 1937
Size: 643 165 134 4594
Fragments arranged by size:
4594 643 165 134
Acc65I G'GTAC_C
Cuts at: 2857 2857
Size: 5536
AclI AA'CG_TT
Cuts at: 1563 3663 4036 1563
Size: 2100 373 3063
Fragments arranged by size:
3063 2100 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3599 4983 5251 1632
Size: 1967 1384 268 1917
Fragments arranged by size:
1967 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5339
AflIII A'CryG_T
Cuts at: 1203 2604 4435 5412 1203
Size: 1401 1831 977 1327
Fragments arranged by size:
1831 1401 1327 977
AgeI A'CCGG_T
Cuts at: 4367 4367
Size: 5536
AhdI GACnn_n'nnGTC
Cuts at: 4265 4265
Size: 5536
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5536
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5536
AlwNI CAG_nnn'CTG
Cuts at: 3279 4851 3279
Size: 1572 3964
ApaI G_GGCC'C
Cuts at: 2666 2831 2666
Size: 165 5371
ApaLI G'TGCA_C
Cuts at: 3593 4749 3593
Size: 1156 4380
ApoI r'AATT_y
Cuts at: 1493 2845 3071 3223 5332 1493
Size: 1352 226 152 2109 1697
Fragments arranged by size:
2109 1697 1352 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4090 160
Size: 872 3058 1606
Fragments arranged by size:
3058 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5536
AvaI C'yCGr_G
Cuts at: 1870 1943 2861 1870
Size: 73 918 4545
Fragments arranged by size:
4545 918 73
AvrII C'CTAG_G
Cuts at: 2260 2275 2260
Size: 15 5521
BamHI G'GATC_C
Cuts at: 2775 2866 3233 2775
Size: 91 367 5078
Fragments arranged by size:
5078 367 91
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2465 2492 2857 4312 618
Size: 325 217 627 678 27 365 1455 1842
Fragments arranged by size:
1842 1455 678 627 365 325 217 27
BanII G_rGCy'C
Cuts at: 1391 2250 2666 2831 2855 1391
Size: 859 416 165 24 4072
Fragments arranged by size:
4072 859 416 165 24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2064 928
Size: 971 165 4400
Fragments arranged by size:
4400 971 165
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3727 3761 1903
Size: 34 1790 34 3678
Fragments arranged by size:
3678 1790 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3428 4638 3428
Size: 1210 4326
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5536
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4147 136
Size: 107 122 71 631 605 2475 1525
Fragments arranged by size:
2475 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5531 5531
Size: 5536
BlpI GC'TnA_GC
Cuts at: 2953 2953
Size: 5536
Bme1580I G_kGCm'C
Cuts at: 1790 2495 2666 2831 3597 4753 1790
Size: 705 171 165 766 1156 2573
Fragments arranged by size:
2573 1156 766 705 171 165
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5536
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3267 4225 442
Size: 416 53 83 399 24 1850 958 1753
Fragments arranged by size:
1850 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5536
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2671 2671
Size: 5536
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2408 4196 1239
Size: 1169 1788 2579
Fragments arranged by size:
2579 1788 1169
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2743 3661 4526 4824 2743
Size: 918 865 298 3455
Fragments arranged by size:
3455 918 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4199 882
Size: 617 2700 2219
Fragments arranged by size:
2700 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3327 493
Size: 711 2123 2702
Fragments arranged by size:
2702 2123 711
BsaBI GATnn'nnATC
Cuts at: 5530 5530
Size: 5536
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3343 3725 4360 275
Size: 53 83 186 936 1810 382 635 1451
Fragments arranged by size:
1810 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2340 3969 4367 4641 4788 5199 2340
Size: 1629 398 274 147 411 2677
Fragments arranged by size:
2677 1629 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2184 2214 2381 2411 1178
Size: 30 165 30 781 30 167 30 4303
Fragments arranged by size:
4303 781 167 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2579 1186
Size: 1393 4143
BseYI C'CCAG_C
Cuts at: 2487 2805 4739 5235 2487
Size: 318 1934 496 2788
Fragments arranged by size:
2788 1934 496 318
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4963
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2986 3747 4775 1540
Size: 275 186 985 761 1028 2301
Fragments arranged by size:
2301 1028 985 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3597 3682 4753 3597
Size: 85 1071 4380
Fragments arranged by size:
4380 1071 85
BsmI GAATG_Cn'
Cuts at: 3047 3140 3047
Size: 93 5443
BsmBI CGTCTCn'nnnn_
Cuts at: 2704 2704
Size: 5536
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2250 2495 2666 2831 2855 3597 3682
Size: 399 460 245 171 165 24 742 85
Cuts at: 3682 4753 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 460 399 245 171 165
85 24
BspEI T'CCGG_A
Cuts at: 5199 5199
Size: 5536
BspHI T'CATG_A
Cuts at: 3425 5155 3425
Size: 1730 3806
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2135 2290 2891 844
Size: 1291 155 601 3489
Fragments arranged by size:
3489 1291 601 155
BsrBI CCG'CTC
Cuts at: 1394 1823 3423 1394
Size: 429 1600 3507
Fragments arranged by size:
3507 1600 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4031 4205 65
Size: 1183 2783 174 1396
Fragments arranged by size:
2783 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2615 2733 4180 4367 1700
Size: 110 805 118 1447 187 2869
Fragments arranged by size:
2869 1447 805 187 118 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5536
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5536
BssSI C'ACGA_G
Cuts at: 3596 4608 3596
Size: 1012 4524
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5536
BstBI TT'CG_AA
Cuts at: 4340 4340
Size: 5536
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5536
BstYI r'GATC_y
Cuts at: 1972 2775 2866 3233 3618 3635 5087 5173 5185
Size: 803 91 367 385 17 1452 86 12
Cuts at: 5185 5531 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 803 385 367 346 91 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4984
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4797
Fragments arranged by size:
4797 683 56
BtsI GCAGTG_nn'
Cuts at: 2220 3052 3846 3866 2220
Size: 832 794 20 3890
Fragments arranged by size:
3890 832 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5536
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5501
DraI TTT'AAA
Cuts at: 2840 2840
Size: 5536
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4543 809
Size: 507 3227 1802
Fragments arranged by size:
3227 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2983 3872 5501
Size: 54 1425 50 275 18 1153 889 1629
Cuts at: 5501 8
Size: 43
Fragments arranged by size:
1629 1425 1153 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2983 1537
Size: 275 1171 4090
Fragments arranged by size:
4090 1171 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3466 1353
Size: 2113 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4123 4497 4643 387
Size: 1064 138 2534 374 146 1280
Fragments arranged by size:
2534 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2408 3599 4196 4983 5251 1239
Size: 393 776 1191 597 787 268 1524
Fragments arranged by size:
1524 1191 787 776 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2215 2662 2663 2827 2215
Size: 447 1 164 4924
Fragments arranged by size:
4924 447 164 1
EcoRI G'AATT_C
Cuts at: 2845 3223 2845
Size: 378 5158
FspI TGC'GCA
Cuts at: 4042 4042
Size: 5536
HaeII r_GCGC'y
Cuts at: 1678 4683 1678
Size: 3005 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2184 2216 2381 2413 4177
Size: 32 135 32 612 32 165 32 1764
Cuts at: 4177 4209 4251 4283 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1764 612 165 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2880 3126 669
Size: 988 281 942 246 3079
Fragments arranged by size:
3079 988 942 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5536
HpaI GTT'AAC
Cuts at: 3126 3126
Size: 5536
KpnI G_GTAC'C
Cuts at: 2861 2861
Size: 5536
MfeI C'AATT_G
Cuts at: 3135 3135
Size: 5536
MluI A'CGCG_T
Cuts at: 5412 5412
Size: 5536
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2572 4650 4834 1305
Size: 1267 2078 184 2007
Fragments arranged by size:
2078 2007 1267 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3714
Fragments arranged by size:
3714 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2370 3494 3853 4012 518
Size: 648 1204 1124 359 159 2042
Fragments arranged by size:
2042 1204 1124 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2952 3629 4777 5022 1606
Size: 1346 677 1148 245 2120
Fragments arranged by size:
2120 1346 1148 677 245
NaeI GCC'GGC
Cuts at: 2617 2617
Size: 5536
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4984
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5536
NgoMIV G'CCGG_C
Cuts at: 2615 2615
Size: 5536
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5536
NotI GC'GGCC_GC
Cuts at: 2983 2983
Size: 5536
NspI r_CATG'y
Cuts at: 1132 1229 2608 2894 4439 1132
Size: 97 1379 286 1545 2229
Fragments arranged by size:
2229 1545 1379 286 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5536
PciI A'CATG_T
Cuts at: 2604 4435 2604
Size: 1831 3705
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5536
PmeI GTTT'AAAC
Cuts at: 2840 2840
Size: 5536
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3605 3637 5118 5150 3605
Size: 32 1481 32 3991
Fragments arranged by size:
3991 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2215 2215
Size: 5536
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5536
PsiI TTA'TAA
Cuts at: 3106 3106
Size: 5536
PspOMI G'GGCC_C
Cuts at: 2662 2827 2662
Size: 165 5371
PstI C_TGCA'G
Cuts at: 830 2397 2888 830
Size: 1567 491 3478
Fragments arranged by size:
3478 1567 491
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5536
SalI G'TCGA_C
Cuts at: 1936 2878 1936
Size: 942 4594
SbfI CC_TGCA'GG
Cuts at: 2888 2888
Size: 5536
ScaI AGT'ACT
Cuts at: 3784 5496 3784
Size: 1712 3824
SfcI C'TryA_G
Cuts at: 826 1045 2393 2884 4019 4700 4891 826
Size: 219 1348 491 1135 681 191 1471
Fragments arranged by size:
1471 1348 1135 681 491 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5536
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5536
SmaI CCC'GGG
Cuts at: 1872 2863 1872
Size: 991 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2758 3640 4541 4803 820
Size: 197 926 815 882 901 262 1553
Fragments arranged by size:
1553 926 901 882 815 262 197
SnaBI TAC'GTA
Cuts at: 493 3327 493
Size: 2834 2702
SpeI A'CTAG_T
Cuts at: 152 2164 152
Size: 2012 3524
SphI G_CATG'C
Cuts at: 1132 2894 1132
Size: 1762 3774
SspI AAT'ATT
Cuts at: 5 52 3460 5
Size: 47 3408 2081
Fragments arranged by size:
3408 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5536
StyI C'CwwG_G
Cuts at: 513 1065 2040 2076 2260 2275 2975 513
Size: 552 975 36 184 15 700 3074
Fragments arranged by size:
3074 975 700 552 184 36 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2369 3563 3580 3733 3918 4363 325
Size: 1255 789 1194 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1194 789 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3782 5494 96
Size: 274 80 33 51 1443 1805 1712 138
Fragments arranged by size:
1805 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5496 5528 5496
Size: 32 5504
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5536
XbaI T'CTAG_A
Cuts at: 2872 2872
Size: 5536
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5536
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5536
XmaI C'CCGG_G
Cuts at: 1870 2861 1870
Size: 991 4545
XmnI GAAnn'nnTTC
Cuts at: 3665 3665
Size: 5536
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3344 276
Size: 53 83 186 936 1810 2468
Fragments arranged by size:
2468 1810 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI