Restriction map for
pFN21AE1764
(Circular) (Six-base) MAPSORT of: pFN21AE1764.seq Check: 2069 from: 1 to: 5515
pFN21AE1764 5515 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3325 278
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2858 1937
Size: 921 4594
Acc65I G'GTAC_C
Cuts at: 2836 2836
Size: 5515
AclI AA'CG_TT
Cuts at: 1563 3642 4015 1563
Size: 2079 373 3063
Fragments arranged by size:
3063 2079 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3578 4962 5230 1632
Size: 1946 1384 268 1917
Fragments arranged by size:
1946 1917 1384 268
AfeI AGC'GCT
Cuts at: 2600 2600
Size: 5515
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5318
AflIII A'CryG_T
Cuts at: 1203 4414 5391 1203
Size: 3211 977 1327
Fragments arranged by size:
3211 1327 977
AgeI A'CCGG_T
Cuts at: 2769 4346 2769
Size: 1577 3938
AhdI GACnn_n'nnGTC
Cuts at: 4244 4244
Size: 5515
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5515
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5515
AlwNI CAG_nnn'CTG
Cuts at: 3258 4830 3258
Size: 1572 3943
ApaLI G'TGCA_C
Cuts at: 3572 4728 3572
Size: 1156 4359
ApoI r'AATT_y
Cuts at: 1493 2180 2824 3050 3202 5311 1493
Size: 687 644 226 152 2109 1697
Fragments arranged by size:
2109 1697 687 644 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4069 160
Size: 872 3037 1606
Fragments arranged by size:
3037 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
AvaI C'yCGr_G
Cuts at: 1870 1943 2077 2840 1870
Size: 73 134 763 4545
Fragments arranged by size:
4545 763 134 73
BamHI G'GATC_C
Cuts at: 2845 3212 2845
Size: 367 5148
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2084 2164 2608 2836 4291
Size: 325 217 627 297 80 444 228 1455
Cuts at: 4291 618
Size: 1842
Fragments arranged by size:
1842 1455 627 444 325 297 228 217
80
BanII G_rGCy'C
Cuts at: 1391 2424 2577 2697 2834 1391
Size: 1033 153 120 137 4072
Fragments arranged by size:
4072 1033 153 137 120
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2136 928
Size: 971 237 4307
Fragments arranged by size:
4307 971 237
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3706 3740 1903
Size: 34 1769 34 3678
Fragments arranged by size:
3678 1769 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3407 4617 3407
Size: 1210 4305
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5515
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2442 4126 136
Size: 107 122 71 631 605 770 1684 1525
Fragments arranged by size:
1684 1525 770 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5510 5510
Size: 5515
BlpI GC'TnA_GC
Cuts at: 2756 2932 2756
Size: 176 5339
Bme1580I G_kGCm'C
Cuts at: 1790 2087 2611 3576 4732 1790
Size: 297 524 965 1156 2573
Fragments arranged by size:
2573 1156 965 524 297
BmgBI CAC'GTC
Cuts at: 1318 2704 1318
Size: 1386 4129
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3246 4204 442
Size: 416 53 83 399 24 1829 958 1753
Fragments arranged by size:
1829 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2057 2323 2621 4175 1239
Size: 818 266 298 1554 2579
Fragments arranged by size:
2579 1554 818 298 266
Bpu10I CC'TnA_GC
Cuts at: 2691 2691
Size: 5515
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3640 4505 4803 3640
Size: 865 298 4352
Fragments arranged by size:
4352 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4178 882
Size: 617 2679 2219
Fragments arranged by size:
2679 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3306 493
Size: 711 2102 2702
Fragments arranged by size:
2702 2102 711
BsaBI GATnn'nnATC
Cuts at: 5509 5509
Size: 5515
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2060 2133 3322 3704
Size: 53 83 186 936 527 73 1189 382
Cuts at: 3704 4339 275
Size: 635 1451
Fragments arranged by size:
1451 1189 936 635 527 382 186 83
73 53
BsaWI w'CCGG_w
Cuts at: 2769 3948 4346 4620 4767 5178 2769
Size: 1179 398 274 147 411 3106
Fragments arranged by size:
3106 1179 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2619 2649 2736 2766 1178
Size: 30 165 30 1216 30 87 30 3927
Fragments arranged by size:
3927 1216 165 87 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2624 1186
Size: 1438 4077
BseYI C'CCAG_C
Cuts at: 4718 5214 4718
Size: 496 5019
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4942
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2965 3726 4754 1540
Size: 275 186 964 761 1028 2301
Fragments arranged by size:
2301 1028 964 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2525 2757 3576 3661 4732 2525
Size: 232 819 85 1071 3308
Fragments arranged by size:
3308 1071 819 232 85
BsmI GAATG_Cn'
Cuts at: 3026 3119 3026
Size: 93 5422
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2424 2525 2577 2611 2697 2757
Size: 399 297 337 101 52 34 86 60
Cuts at: 2757 2834 3576 3661 4732 1391
Size: 77 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 337 297 101 86
85 77 60 52 34
BspEI T'CCGG_A
Cuts at: 5178 5178
Size: 5515
BspHI T'CATG_A
Cuts at: 3404 5134 3404
Size: 1730 3785
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2870 844
Size: 2026 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2041 3402 1394
Size: 429 218 1361 3507
Fragments arranged by size:
3507 1361 429 218
BsrDI GCAATG_nn'
Cuts at: 65 1248 4010 4184 65
Size: 1183 2762 174 1396
Fragments arranged by size:
2762 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2769 4159 4346 1700
Size: 110 959 1390 187 2869
Fragments arranged by size:
2869 1390 959 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5515
BssHII G'CGCG_C
Cuts at: 1925 2026 1925
Size: 101 5414
BssSI C'ACGA_G
Cuts at: 3575 4587 3575
Size: 1012 4503
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5515
BstBI TT'CG_AA
Cuts at: 4319 4319
Size: 5515
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5515
BstYI r'GATC_y
Cuts at: 1972 2109 2353 2845 3212 3597 3614 5066 5152
Size: 137 244 492 367 385 17 1452 86
Cuts at: 5152 5164 5510 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 492 385 367 346 244 137
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4024
Fragments arranged by size:
4024 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4776
Fragments arranged by size:
4776 683 56
BtsI GCAGTG_nn'
Cuts at: 2026 3031 3825 3845 2026
Size: 1005 794 20 3696
Fragments arranged by size:
3696 1005 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5515
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5480
DraI TTT'AAA
Cuts at: 2819 2819
Size: 5515
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4522 809
Size: 507 3206 1802
Fragments arranged by size:
3206 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2050 2237 2962
Size: 54 1425 50 275 18 220 187 725
Cuts at: 2962 3851 5480 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 725 275 220 187 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2962 1537
Size: 275 1150 4090
Fragments arranged by size:
4090 1150 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2147 2589 3445 1353
Size: 794 442 856 3423
Fragments arranged by size:
3423 856 794 442
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2280 4102 4476 4622 387
Size: 1064 138 691 1822 374 146 1280
Fragments arranged by size:
1822 1280 1064 691 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2323 2621 3578 4175 4962 5230
Size: 393 425 266 298 957 597 787 268
Cuts at: 5230 1239
Size: 1524
Fragments arranged by size:
1524 957 787 597 425 393 298 268
266
EcoNI CCTnn'n_nnAGG
Cuts at: 2651 2651
Size: 5515
EcoO109I rG'GnC_Cy
Cuts at: 2289 2558 2289
Size: 269 5246
EcoRI G'AATT_C
Cuts at: 2180 2824 3202 2180
Size: 644 378 4493
Fragments arranged by size:
4493 644 378
FspI TGC'GCA
Cuts at: 4021 4021
Size: 5515
HaeII r_GCGC'y
Cuts at: 1678 2602 4662 1678
Size: 924 2060 2531
Fragments arranged by size:
2531 2060 924
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4156 4188 4230 4262 1373
Size: 32 135 32 2584 32 42 32 2626
Fragments arranged by size:
2626 2584 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2452 2859 3105 669
Size: 988 281 514 407 246 3079
Fragments arranged by size:
3079 988 514 407 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5515
HpaI GTT'AAC
Cuts at: 2452 3105 2452
Size: 653 4862
KpnI G_GTAC'C
Cuts at: 2840 2840
Size: 5515
MfeI C'AATT_G
Cuts at: 3114 3114
Size: 5515
MluI A'CGCG_T
Cuts at: 5391 5391
Size: 5515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4629 4813 1305
Size: 3324 184 2007
Fragments arranged by size:
3324 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2052 10
Size: 54 1425 343 220 3473
Fragments arranged by size:
3473 1425 343 220 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2465 3473 3832 3991 518
Size: 648 1299 1008 359 159 2042
Fragments arranged by size:
2042 1299 1008 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2931 3608 4756 5001 1606
Size: 1325 677 1148 245 2120
Fragments arranged by size:
2120 1325 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4024
Fragments arranged by size:
4024 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5515
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5515
NotI GC'GGCC_GC
Cuts at: 2962 2962
Size: 5515
NspI r_CATG'y
Cuts at: 1132 1229 2873 4418 1132
Size: 97 1644 1545 2229
Fragments arranged by size:
2229 1644 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5515
PciI A'CATG_T
Cuts at: 4414 4414
Size: 5515
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5515
PfoI T'CCnGG_A
Cuts at: 2349 2349
Size: 5515
PmeI GTTT'AAAC
Cuts at: 2819 2819
Size: 5515
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3584 3616 5097 5129 3584
Size: 32 1481 32 3970
Fragments arranged by size:
3970 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2289 2558 2289
Size: 269 5246
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5515
PsiI TTA'TAA
Cuts at: 3085 3085
Size: 5515
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2705 2737 2705
Size: 32 5483
PstI C_TGCA'G
Cuts at: 830 2867 830
Size: 2037 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5515
SalI G'TCGA_C
Cuts at: 1936 2857 1936
Size: 921 4594
SapI GCTCTTCn'nnn_
Cuts at: 2589 2589
Size: 5515
SbfI CC_TGCA'GG
Cuts at: 2867 2867
Size: 5515
ScaI AGT'ACT
Cuts at: 3763 5475 3763
Size: 1712 3803
SfcI C'TryA_G
Cuts at: 826 1045 2863 3998 4679 4870 826
Size: 219 1818 1135 681 191 1471
Fragments arranged by size:
1818 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5515
SmaI CCC'GGG
Cuts at: 1872 2842 1872
Size: 970 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3619 4520 4782 820
Size: 197 926 1676 901 262 1553
Fragments arranged by size:
1676 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3306 493
Size: 2813 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5515
SphI G_CATG'C
Cuts at: 1132 2873 1132
Size: 1741 3774
SspI AAT'ATT
Cuts at: 5 52 3439 5
Size: 47 3387 2081
Fragments arranged by size:
3387 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5515
StyI C'CwwG_G
Cuts at: 513 1065 2004 2088 2954 513
Size: 552 939 84 866 3074
Fragments arranged by size:
3074 939 866 552 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3542 3559 3712 3897 4342 325
Size: 1255 1962 17 153 185 445 1498
Fragments arranged by size:
1962 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2376 3761 5473
Size: 274 80 33 51 1443 399 1385 1712
Cuts at: 5473 96
Size: 138
Fragments arranged by size:
1712 1443 1385 399 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2384 2399 2416 2431 2619 2651 2656 2688 2736
Size: 15 17 15 188 32 5 32 48
Cuts at: 2736 2768 5475 5507 2384
Size: 32 2707 32 2392
Fragments arranged by size:
2707 2392 188 48 32 32 32 32
17 15 15 5
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5515
XbaI T'CTAG_A
Cuts at: 2851 2851
Size: 5515
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2113 2113
Size: 5515
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5515
XmaI C'CCGG_G
Cuts at: 1870 2840 1870
Size: 970 4545
XmnI GAAnn'nnTTC
Cuts at: 3644 3644
Size: 5515
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3323 276
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PvuII
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI