Restriction map for
pFN21AE1753
(Circular) (Six-base) MAPSORT of: pFN21AE1753.seq Check: 6679 from: 1 to: 5485
pFN21AE1753 5485 bp
With 182 enzymes: SgfI *
January 27, 2015 16:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2656 2656
Size: 5485
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3295 278
Size: 53 83 186 936 1759 2468
Fragments arranged by size:
2468 1759 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2828 1937
Size: 891 4594
Acc65I G'GTAC_C
Cuts at: 2806 2806
Size: 5485
AclI AA'CG_TT
Cuts at: 1563 3612 3985 1563
Size: 2049 373 3063
Fragments arranged by size:
3063 2049 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2156 2456 2774 3548 4932 5200 1632
Size: 524 300 318 774 1384 268 1917
Fragments arranged by size:
1917 1384 774 524 318 300 268
AfeI AGC'GCT
Cuts at: 2458 2458
Size: 5485
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5288
AflIII A'CryG_T
Cuts at: 1203 4384 5361 1203
Size: 3181 977 1327
Fragments arranged by size:
3181 1327 977
AgeI A'CCGG_T
Cuts at: 4316 4316
Size: 5485
AhdI GACnn_n'nnGTC
Cuts at: 4214 4214
Size: 5485
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5485
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5485
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2412 2444 2412
Size: 32 5453
AlwNI CAG_nnn'CTG
Cuts at: 2752 3228 4800 2752
Size: 476 1572 3437
Fragments arranged by size:
3437 1572 476
ApaLI G'TGCA_C
Cuts at: 3542 4698 3542
Size: 1156 4329
ApoI r'AATT_y
Cuts at: 1493 2675 2794 3020 3172 5281 1493
Size: 1182 119 226 152 2109 1697
Fragments arranged by size:
2109 1697 1182 226 152 119
AseI AT'TA_AT
Cuts at: 160 1032 4039 160
Size: 872 3007 1606
Fragments arranged by size:
3007 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5485
AvaI C'yCGr_G
Cuts at: 1870 1943 2810 1870
Size: 73 867 4545
Fragments arranged by size:
4545 867 73
BamHI G'GATC_C
Cuts at: 2178 2815 3182 2178
Size: 637 367 4481
Fragments arranged by size:
4481 637 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2736 2806 4261 618
Size: 325 217 627 949 70 1455 1842
Fragments arranged by size:
1842 1455 949 627 325 217 70
BanII G_rGCy'C
Cuts at: 1391 2804 1391
Size: 1413 4072
BbeI G_GCGC'C
Cuts at: 2740 2740
Size: 5485
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4514
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3676 3710 1903
Size: 34 1739 34 3678
Fragments arranged by size:
3678 1739 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3377 4587 3377
Size: 1210 4275
BclI T'GATC_A
Cuts at: 1548 2158 1548
Size: 610 4875
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4096 136
Size: 107 122 71 631 605 2424 1525
Fragments arranged by size:
2424 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5480 5480
Size: 5485
BlpI GC'TnA_GC
Cuts at: 2745 2902 2745
Size: 157 5328
Bme1580I G_kGCm'C
Cuts at: 1790 3546 4702 1790
Size: 1756 1156 2573
Fragments arranged by size:
2573 1756 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5485
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2026 2302 3216
Size: 416 53 83 399 24 609 276 914
Cuts at: 3216 4174 442
Size: 958 1753
Fragments arranged by size:
1753 958 914 609 416 399 276 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5485
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4145 1239
Size: 2906 2579
Bpu10I CC'TnA_GC
Cuts at: 2043 2043
Size: 5485
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2225 2621 3610 4475 4773 2225
Size: 396 989 865 298 2937
Fragments arranged by size:
2937 989 865 396 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4148 882
Size: 617 2649 2219
Fragments arranged by size:
2649 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3276 493
Size: 711 2072 2702
Fragments arranged by size:
2702 2072 711
BsaBI GATnn'nnATC
Cuts at: 5479 5479
Size: 5485
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2737 3292 3674 4309
Size: 53 83 186 936 1204 555 382 635
Cuts at: 4309 275
Size: 1451
Fragments arranged by size:
1451 1204 936 635 555 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3918 4316 4590 4737 5148 3918
Size: 398 274 147 411 4255
Fragments arranged by size:
4255 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2587 2617 1178
Size: 30 165 30 1184 30 4046
Fragments arranged by size:
4046 1184 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2595 1186
Size: 1409 4076
BseYI C'CCAG_C
Cuts at: 2740 4688 5184 2740
Size: 1948 496 3041
Fragments arranged by size:
3041 1948 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2426 1163
Size: 573 690 4222
Fragments arranged by size:
4222 690 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2935 3696 4724 1540
Size: 275 186 934 761 1028 2301
Fragments arranged by size:
2301 1028 934 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2049 2241 2637 3546 3631 4702 2049
Size: 192 396 909 85 1071 2832
Fragments arranged by size:
2832 1071 909 396 192 85
BsmI GAATG_Cn'
Cuts at: 2996 3089 2996
Size: 93 5392
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2241 2637 2804 3546 3631 4702
Size: 399 259 192 396 167 742 85 1071
Cuts at: 4702 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 396 259 192 167
85
BspEI T'CCGG_A
Cuts at: 5148 5148
Size: 5485
BspHI T'CATG_A
Cuts at: 3374 5104 3374
Size: 1730 3755
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2656 2840 844
Size: 1812 184 3489
Fragments arranged by size:
3489 1812 184
BsrBI CCG'CTC
Cuts at: 1394 1823 3372 1394
Size: 429 1549 3507
Fragments arranged by size:
3507 1549 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2067 3980 4154 65
Size: 1183 819 1913 174 1396
Fragments arranged by size:
1913 1396 1183 819 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4129 4316 1700
Size: 110 2319 187 2869
Fragments arranged by size:
2869 2319 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5485
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5485
BssSI C'ACGA_G
Cuts at: 2544 3545 4557 2544
Size: 1001 1012 3472
Fragments arranged by size:
3472 1012 1001
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5485
BstBI TT'CG_AA
Cuts at: 4289 4289
Size: 5485
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5485
BstYI r'GATC_y
Cuts at: 1972 2178 2815 3182 3567 3584 5036 5122 5134
Size: 206 637 367 385 17 1452 86 12
Cuts at: 5134 5480 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 637 385 367 346 206 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2174 2174
Size: 5485
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4933
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4746
Fragments arranged by size:
4746 683 56
BtsI GCAGTG_nn'
Cuts at: 2241 3001 3795 3815 2241
Size: 760 794 20 3911
Fragments arranged by size:
3911 794 760 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5485
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5450
DraI TTT'AAA
Cuts at: 2789 2789
Size: 5485
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4492 809
Size: 507 3176 1802
Fragments arranged by size:
3176 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2424 2932 3821
Size: 54 1425 50 275 18 594 508 889
Cuts at: 3821 5450 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 594 508 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2932 1537
Size: 275 1120 4090
Fragments arranged by size:
4090 1120 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2613 3415 1353
Size: 1260 802 3423
Fragments arranged by size:
3423 1260 802
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4072 4446 4592 387
Size: 1064 138 2483 374 146 1280
Fragments arranged by size:
2483 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2456 2774 3548 4145 4932 5200
Size: 393 524 300 318 774 597 787 268
Cuts at: 5200 1239
Size: 1524
Fragments arranged by size:
1524 787 774 597 524 393 318 300
268
EcoO109I rG'GnC_Cy
Cuts at: 2249 2249
Size: 5485
EcoRI G'AATT_C
Cuts at: 2794 3172 2794
Size: 378 5107
FspI TGC'GCA
Cuts at: 3991 3991
Size: 5485
HaeII r_GCGC'y
Cuts at: 1678 2086 2460 2740 4632 1678
Size: 408 374 280 1892 2531
Fragments arranged by size:
2531 1892 408 374 280
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2072 2104 2587 2619 4126
Size: 32 135 32 500 32 483 32 1507
Cuts at: 4126 4158 4200 4232 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1507 500 483 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2098 2829 3075 669
Size: 988 281 160 731 246 3079
Fragments arranged by size:
3079 988 731 281 246 160
HindIII A'AGCT_T
Cuts at: 748 2772 748
Size: 2024 3461
HpaI GTT'AAC
Cuts at: 3075 3075
Size: 5485
KasI G'GCGC_C
Cuts at: 2736 2736
Size: 5485
KpnI G_GTAC'C
Cuts at: 2810 2810
Size: 5485
MfeI C'AATT_G
Cuts at: 3084 3084
Size: 5485
MluI A'CGCG_T
Cuts at: 5361 5361
Size: 5485
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4599 4783 1305
Size: 3294 184 2007
Fragments arranged by size:
3294 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3663
Fragments arranged by size:
3663 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3443 3802 3961 518
Size: 648 2277 359 159 2042
Fragments arranged by size:
2277 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2901 3578 4726 4971 1606
Size: 1295 677 1148 245 2120
Fragments arranged by size:
2120 1295 1148 677 245
NarI GG'CG_CC
Cuts at: 2737 2737
Size: 5485
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4933
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5485
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5485
NotI GC'GGCC_GC
Cuts at: 2932 2932
Size: 5485
NspI r_CATG'y
Cuts at: 1132 1229 2843 4388 1132
Size: 97 1614 1545 2229
Fragments arranged by size:
2229 1614 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5485
PciI A'CATG_T
Cuts at: 4384 4384
Size: 5485
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5485
PmeI GTTT'AAAC
Cuts at: 2789 2789
Size: 5485
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3554 3586 5067 5099 3554
Size: 32 1481 32 3940
Fragments arranged by size:
3940 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2249 2249
Size: 5485
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5485
PsiI TTA'TAA
Cuts at: 3055 3055
Size: 5485
PstI C_TGCA'G
Cuts at: 830 2353 2837 830
Size: 1523 484 3478
Fragments arranged by size:
3478 1523 484
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5485
SalI G'TCGA_C
Cuts at: 1936 2827 1936
Size: 891 4594
SbfI CC_TGCA'GG
Cuts at: 2837 2837
Size: 5485
ScaI AGT'ACT
Cuts at: 3733 5445 3733
Size: 1712 3773
SfcI C'TryA_G
Cuts at: 826 1045 2349 2833 3968 4649 4840 826
Size: 219 1304 484 1135 681 191 1471
Fragments arranged by size:
1471 1304 1135 681 484 219 191
SfoI GGC'GCC
Cuts at: 2738 2738
Size: 5485
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5485
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5485
SmaI CCC'GGG
Cuts at: 1872 2812 1872
Size: 940 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2240 2636 3589 4490 4752 820
Size: 197 926 297 396 953 901 262 1553
Fragments arranged by size:
1553 953 926 901 396 297 262 197
SnaBI TAC'GTA
Cuts at: 493 3276 493
Size: 2783 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5485
SphI G_CATG'C
Cuts at: 1132 2843 1132
Size: 1711 3774
SspI AAT'ATT
Cuts at: 5 52 3409 5
Size: 47 3357 2081
Fragments arranged by size:
3357 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5485
StyI C'CwwG_G
Cuts at: 513 1065 2073 2924 513
Size: 552 1008 851 3074
Fragments arranged by size:
3074 1008 851 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3512 3529 3682 3867 4312 325
Size: 1255 1932 17 153 185 445 1498
Fragments arranged by size:
1932 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3731 5443 96
Size: 274 80 33 51 1443 1754 1712 138
Fragments arranged by size:
1754 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5445 5477 5445
Size: 32 5453
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5485
XbaI T'CTAG_A
Cuts at: 2821 2821
Size: 5485
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5485
XmaI C'CCGG_G
Cuts at: 1870 2810 1870
Size: 940 4545
XmnI GAAnn'nnTTC
Cuts at: 3614 3614
Size: 5485
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3293 276
Size: 53 83 186 936 1759 2468
Fragments arranged by size:
2468 1759 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI