Restriction map for
pFN21AE1752
(Circular) (Six-base) MAPSORT of: pFN21AE1752.seq Check: 5529 from: 1 to: 5476
pFN21AE1752 5476 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3286 278
Size: 53 83 186 936 1750 2468
Fragments arranged by size:
2468 1750 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2819 1937
Size: 882 4594
Acc65I G'GTAC_C
Cuts at: 2797 2797
Size: 5476
AclI AA'CG_TT
Cuts at: 1563 3603 3976 1563
Size: 2040 373 3063
Fragments arranged by size:
3063 2040 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2333 2662 3539 4923 5191 1632
Size: 701 329 877 1384 268 1917
Fragments arranged by size:
1917 1384 877 701 329 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5279
AflIII A'CryG_T
Cuts at: 1203 2739 4375 5352 1203
Size: 1536 1636 977 1327
Fragments arranged by size:
1636 1536 1327 977
AgeI A'CCGG_T
Cuts at: 4307 4307
Size: 5476
AhdI GACnn_n'nnGTC
Cuts at: 4205 4205
Size: 5476
AleI CACnn'nnGTG
Cuts at: 1166 2726 1166
Size: 1560 3916
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5476
AlwNI CAG_nnn'CTG
Cuts at: 2691 3219 4791 2691
Size: 528 1572 3376
Fragments arranged by size:
3376 1572 528
ApaI G_GGCC'C
Cuts at: 2050 2634 2050
Size: 584 4892
ApaLI G'TGCA_C
Cuts at: 2171 3533 4689 2171
Size: 1362 1156 2958
Fragments arranged by size:
2958 1362 1156
ApoI r'AATT_y
Cuts at: 1493 2785 3011 3163 5272 1493
Size: 1292 226 152 2109 1697
Fragments arranged by size:
2109 1697 1292 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4030 160
Size: 872 2998 1606
Fragments arranged by size:
2998 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5476
AvaI C'yCGr_G
Cuts at: 1870 1943 2484 2623 2801 1870
Size: 73 541 139 178 4545
Fragments arranged by size:
4545 541 178 139 73
BamHI G'GATC_C
Cuts at: 2806 3173 2806
Size: 367 5109
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2475 2731 2797 4252 618
Size: 325 217 627 688 256 66 1455 1842
Fragments arranged by size:
1842 1455 688 627 325 256 217 66
BanII G_rGCy'C
Cuts at: 1391 2050 2247 2634 2795 1391
Size: 659 197 387 161 4072
Fragments arranged by size:
4072 659 387 197 161
BbeI G_GCGC'C
Cuts at: 2479 2735 2479
Size: 256 5220
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2013 928
Size: 971 114 4391
Fragments arranged by size:
4391 971 114
BbvCI CC'TCA_GC
Cuts at: 2228 2228
Size: 5476
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3667 3701 1903
Size: 34 1730 34 3678
Fragments arranged by size:
3678 1730 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3368 4578 3368
Size: 1210 4266
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5476
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2045 4087
Size: 107 122 71 631 605 337 36 2042
Cuts at: 4087 136
Size: 1525
Fragments arranged by size:
2042 1525 631 605 337 122 107 71
36
BglII A'GATC_T
Cuts at: 5471 5471
Size: 5476
BlpI GC'TnA_GC
Cuts at: 2893 2893
Size: 5476
Bme1580I G_kGCm'C
Cuts at: 1790 2050 2175 2634 3537 4693 1790
Size: 260 125 459 903 1156 2573
Fragments arranged by size:
2573 1156 903 459 260 125
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5476
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2397 3207 4165
Size: 416 53 83 399 24 980 810 958
Cuts at: 4165 442
Size: 1753
Fragments arranged by size:
1753 980 958 810 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5476
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2348 2668 4136 1239
Size: 1109 320 1468 2579
Fragments arranged by size:
2579 1468 1109 320
Bpu10I CC'TnA_GC
Cuts at: 2228 2228
Size: 5476
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3601 4466 4764 3601
Size: 865 298 4313
Fragments arranged by size:
4313 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2273 4139 882
Size: 617 774 1866 2219
Fragments arranged by size:
2219 1866 774 617
BsaAI yAC'GTr
Cuts at: 493 1204 2740 3267 493
Size: 711 1536 527 2702
Fragments arranged by size:
2702 1536 711 527
BsaBI GATnn'nnATC
Cuts at: 5470 5470
Size: 5476
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2476 2732 3283 3665
Size: 53 83 186 936 943 256 551 382
Cuts at: 3665 4300 275
Size: 635 1451
Fragments arranged by size:
1451 943 936 635 551 382 256 186
83 53
BsaWI w'CCGG_w
Cuts at: 3909 4307 4581 4728 5139 3909
Size: 398 274 147 411 4246
Fragments arranged by size:
4246 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5251
Fragments arranged by size:
5251 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5476
BseYI C'CCAG_C
Cuts at: 2211 2223 2239 2372 2451 2567 4679 5175 2211
Size: 12 16 133 79 116 2112 496 2512
Fragments arranged by size:
2512 2112 496 133 116 79 16 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2458 2677 1163
Size: 573 722 219 3962
Fragments arranged by size:
3962 722 573 219
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2390 2589 2767 2771 2775 2926
Size: 275 186 389 199 178 4 4 151
Cuts at: 2926 3687 4715 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 389 275 199 186 178
151 4 4
BsiHKAI G_wGCw'C
Cuts at: 2175 3537 3622 4693 2175
Size: 1362 85 1071 2958
Fragments arranged by size:
2958 1362 1071 85
BsmI GAATG_Cn'
Cuts at: 2987 3080 2987
Size: 93 5383
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2050 2175 2247 2634 2795 3537 3622
Size: 399 260 125 72 387 161 742 85
Cuts at: 3622 4693 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 387 260 161 125
85 72
BspEI T'CCGG_A
Cuts at: 5139 5139
Size: 5476
BspHI T'CATG_A
Cuts at: 3365 5095 3365
Size: 1730 3746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2831 844
Size: 1987 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3363 1394
Size: 429 1540 3507
Fragments arranged by size:
3507 1540 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3971 4145 65
Size: 1183 2723 174 1396
Fragments arranged by size:
2723 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2447 2762 4120 4307 1700
Size: 110 637 315 1358 187 2869
Fragments arranged by size:
2869 1358 637 315 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5476
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5476
BssSI C'ACGA_G
Cuts at: 3536 4548 3536
Size: 1012 4464
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2577 794
Size: 1783 3693
BstBI TT'CG_AA
Cuts at: 4280 4280
Size: 5476
BstEII G'GTnAC_C
Cuts at: 1184 2758 1184
Size: 1574 3902
BstXI CCAn_nnnn'nTGG
Cuts at: 2728 2728
Size: 5476
BstYI r'GATC_y
Cuts at: 1972 2806 3173 3558 3575 5027 5113 5125 5471
Size: 834 367 385 17 1452 86 12 346
Cuts at: 5471 1972
Size: 1977
Fragments arranged by size:
1977 1452 834 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2715 513
Size: 552 939 711 3274
Fragments arranged by size:
3274 939 711 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4737
Fragments arranged by size:
4737 683 56
BtsI GCAGTG_nn'
Cuts at: 2024 2129 2200 2992 3786 3806 2024
Size: 105 71 792 794 20 3694
Fragments arranged by size:
3694 794 792 105 71 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5476
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5441
DraI TTT'AAA
Cuts at: 2780 2780
Size: 5476
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4483 809
Size: 507 3167 1802
Fragments arranged by size:
3167 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2387 2445 2586
Size: 54 1425 50 275 18 557 58 141
Cuts at: 2586 2661 2718 2764 2772 2923 3812 5441 8
Size: 75 57 46 8 151 889 1629 43
Fragments arranged by size:
1629 1425 889 557 275 151 141 75
58 57 54 50 46 43 18 8
EagI C'GGCC_G
Cuts at: 1537 1812 2387 2586 2764 2772 2923 1537
Size: 275 575 199 178 8 151 4090
Fragments arranged by size:
4090 575 275 199 178 151 8
EarI CTCTTCn'nnn_
Cuts at: 1353 3406 1353
Size: 2053 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4063 4437 4583 387
Size: 1064 138 2474 374 146 1280
Fragments arranged by size:
2474 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2333 2348 2662 2668 3539 4136 4923
Size: 393 701 15 314 6 871 597 787
Cuts at: 4923 5191 1239
Size: 268 1524
Fragments arranged by size:
1524 871 787 701 597 393 314 268
15 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2749 2749
Size: 5476
EcoO109I rG'GnC_Cy
Cuts at: 2046 2047 2073 2631 2046
Size: 1 26 558 4891
Fragments arranged by size:
4891 558 26 1
EcoRI G'AATT_C
Cuts at: 2785 3163 2785
Size: 378 5098
FspI TGC'GCA
Cuts at: 3982 3982
Size: 5476
HaeII r_GCGC'y
Cuts at: 1678 2413 2479 2735 4623 1678
Size: 735 66 256 1888 2531
Fragments arranged by size:
2531 1888 735 256 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4117 4149 4191 4223 1373
Size: 32 135 32 2545 32 42 32 2626
Fragments arranged by size:
2626 2545 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2820 3066 669
Size: 988 281 882 246 3079
Fragments arranged by size:
3079 988 882 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5476
HpaI GTT'AAC
Cuts at: 3066 3066
Size: 5476
KasI G'GCGC_C
Cuts at: 2475 2731 2475
Size: 256 5220
KpnI G_GTAC'C
Cuts at: 2801 2801
Size: 5476
MfeI C'AATT_G
Cuts at: 3075 3075
Size: 5476
MluI A'CGCG_T
Cuts at: 5352 5352
Size: 5476
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4590 4774 1305
Size: 3285 184 2007
Fragments arranged by size:
3285 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2720 10
Size: 54 1425 343 888 2766
Fragments arranged by size:
2766 1425 888 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2525 2726 3434 3793 3952 518
Size: 648 1359 201 708 359 159 2042
Fragments arranged by size:
2042 1359 708 648 359 201 159
MspA1I CmG'CkG
Cuts at: 1606 2215 2227 2353 2726 2892 3569 4717 4962
Size: 609 12 126 373 166 677 1148 245
Cuts at: 4962 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 609 373 245 166 126
12
NaeI GCC'GGC
Cuts at: 2449 2449
Size: 5476
NarI GG'CG_CC
Cuts at: 2476 2732 2476
Size: 256 5220
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3985
Fragments arranged by size:
3985 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5476
NgoMIV G'CCGG_C
Cuts at: 2447 2447
Size: 5476
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5476
NotI GC'GGCC_GC
Cuts at: 2923 2923
Size: 5476
NspI r_CATG'y
Cuts at: 1132 1229 2530 2834 4379 1132
Size: 97 1301 304 1545 2229
Fragments arranged by size:
2229 1545 1301 304 97
PasI CC'CwG_GG
Cuts at: 1348 2041 1348
Size: 693 4783
PciI A'CATG_T
Cuts at: 4375 4375
Size: 5476
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2206 2218 2254 2419 2515 1108
Size: 1098 12 36 165 96 4069
Fragments arranged by size:
4069 1098 165 96 36 12
PfoI T'CCnGG_A
Cuts at: 2744 2744
Size: 5476
PmeI GTTT'AAAC
Cuts at: 2780 2780
Size: 5476
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3545 3577 5058 5090 3545
Size: 32 1481 32 3931
Fragments arranged by size:
3931 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2073 2073
Size: 5476
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5476
PsiI TTA'TAA
Cuts at: 3046 3046
Size: 5476
PspOMI G'GGCC_C
Cuts at: 2046 2630 2046
Size: 584 4892
PstI C_TGCA'G
Cuts at: 830 2136 2828 830
Size: 1306 692 3478
Fragments arranged by size:
3478 1306 692
PvuI CG_AT'CG
Cuts at: 2001 2771 2001
Size: 770 4706
PvuII CAG'CTG
Cuts at: 2227 2353 2227
Size: 126 5350
SalI G'TCGA_C
Cuts at: 1936 2818 1936
Size: 882 4594
SanDI GG'GwC_CC
Cuts at: 2073 2073
Size: 5476
SbfI CC_TGCA'GG
Cuts at: 2828 2828
Size: 5476
ScaI AGT'ACT
Cuts at: 3724 5436 3724
Size: 1712 3764
SfcI C'TryA_G
Cuts at: 826 1045 2132 2824 3959 4640 4831 826
Size: 219 1087 692 1135 681 191 1471
Fragments arranged by size:
1471 1135 1087 692 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2045 2045
Size: 5476
SfoI GGC'GCC
Cuts at: 2477 2733 2477
Size: 256 5220
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5476
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5476
SmaI CCC'GGG
Cuts at: 1872 2486 2803 1872
Size: 614 317 4545
Fragments arranged by size:
4545 614 317
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3580 4481 4743 820
Size: 197 926 1637 901 262 1553
Fragments arranged by size:
1637 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3267 493
Size: 2774 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5476
SphI G_CATG'C
Cuts at: 1132 2834 1132
Size: 1702 3774
SspI AAT'ATT
Cuts at: 5 52 3400 5
Size: 47 3348 2081
Fragments arranged by size:
3348 2081 47
StuI AGG'CCT
Cuts at: 1517 2012 1517
Size: 495 4981
StyI C'CwwG_G
Cuts at: 513 1065 2004 2595 2915 513
Size: 552 939 591 320 3074
Fragments arranged by size:
3074 939 591 552 320
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2173 2190 3503 3520 3673 3858 4303
Size: 1255 593 17 1313 17 153 185 445
Cuts at: 4303 325
Size: 1498
Fragments arranged by size:
1498 1313 1255 593 445 185 153 17
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3722 5434 96
Size: 274 80 33 51 1443 1745 1712 138
Fragments arranged by size:
1745 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5436 5468 5436
Size: 32 5444
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5476
XbaI T'CTAG_A
Cuts at: 2812 2812
Size: 5476
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2389 2389
Size: 5476
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5476
XmaI C'CCGG_G
Cuts at: 1870 2484 2801 1870
Size: 614 317 4545
Fragments arranged by size:
4545 614 317
XmnI GAAnn'nnTTC
Cuts at: 3605 3605
Size: 5476
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3284 276
Size: 53 83 186 936 1750 2468
Fragments arranged by size:
2468 1750 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SanDI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PspXI PsrI
RsrII SacI SacII SapI SexAI SrfI SwaI