Restriction map for
pFN21AE1751
(Circular) (Six-base) MAPSORT of: pFN21AE1751.seq Check: 254 from: 1 to: 5467
pFN21AE1751 5467 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2511 2511
Size: 5467
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3277 278
Size: 53 83 186 936 1741 2468
Fragments arranged by size:
2468 1741 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2424 2810 1937
Size: 487 386 4594
Fragments arranged by size:
4594 487 386
Acc65I G'GTAC_C
Cuts at: 2788 2788
Size: 5467
AclI AA'CG_TT
Cuts at: 1563 3594 3967 1563
Size: 2031 373 3063
Fragments arranged by size:
3063 2031 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3530 4914 5182 1632
Size: 1898 1384 268 1917
Fragments arranged by size:
1917 1898 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5270
AflIII A'CryG_T
Cuts at: 1203 4366 5343 1203
Size: 3163 977 1327
Fragments arranged by size:
3163 1327 977
AgeI A'CCGG_T
Cuts at: 4298 4298
Size: 5467
AhdI GACnn_n'nnGTC
Cuts at: 4196 4196
Size: 5467
AleI CACnn'nnGTG
Cuts at: 1166 2622 1166
Size: 1456 4011
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2640 1184
Size: 1456 4011
AlwNI CAG_nnn'CTG
Cuts at: 2512 2638 3210 4782 2512
Size: 126 572 1572 3197
Fragments arranged by size:
3197 1572 572 126
ApaI G_GGCC'C
Cuts at: 2095 2322 2095
Size: 227 5240
ApaLI G'TGCA_C
Cuts at: 3524 4680 3524
Size: 1156 4311
ApoI r'AATT_y
Cuts at: 1493 2776 3002 3154 5263 1493
Size: 1283 226 152 2109 1697
Fragments arranged by size:
2109 1697 1283 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4021 160
Size: 872 2989 1606
Fragments arranged by size:
2989 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5467
AvaI C'yCGr_G
Cuts at: 1870 1943 2206 2225 2792 1870
Size: 73 263 19 567 4545
Fragments arranged by size:
4545 567 263 73 19
BamHI G'GATC_C
Cuts at: 2797 3164 2797
Size: 367 5100
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2696 2788 4243 618
Size: 325 217 627 909 92 1455 1842
Fragments arranged by size:
1842 1455 909 627 325 217 92
BanII G_rGCy'C
Cuts at: 1391 2095 2179 2322 2477 2786 1391
Size: 704 84 143 155 309 4072
Fragments arranged by size:
4072 704 309 155 143 84
BbeI G_GCGC'C
Cuts at: 2700 2700
Size: 5467
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2137 2425 928
Size: 971 238 288 3970
Fragments arranged by size:
3970 971 288 238
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3658 3692 1903
Size: 34 1721 34 3678
Fragments arranged by size:
3678 1721 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3359 4569 3359
Size: 1210 4257
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5467
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2352 2540 4078
Size: 107 122 71 631 605 680 188 1538
Cuts at: 4078 136
Size: 1525
Fragments arranged by size:
1538 1525 680 631 605 188 122 107
71
BglII A'GATC_T
Cuts at: 5462 5462
Size: 5467
BlpI GC'TnA_GC
Cuts at: 2884 2884
Size: 5467
Bme1580I G_kGCm'C
Cuts at: 1790 2095 2322 2671 3528 4684 1790
Size: 305 227 349 857 1156 2573
Fragments arranged by size:
2573 1156 857 349 305 227
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5467
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3198 4156 442
Size: 416 53 83 399 24 1781 958 1753
Fragments arranged by size:
1781 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5467
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2439 4127 1239
Size: 1200 1688 2579
Fragments arranged by size:
2579 1688 1200
Bpu10I CC'TnA_GC
Cuts at: 2324 2375 2476 2324
Size: 51 101 5315
Fragments arranged by size:
5315 101 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3592 4457 4755 3592
Size: 865 298 4304
Fragments arranged by size:
4304 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4130 882
Size: 617 2631 2219
Fragments arranged by size:
2631 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3258 493
Size: 711 2054 2702
Fragments arranged by size:
2702 2054 711
BsaBI GATnn'nnATC
Cuts at: 5461 5461
Size: 5467
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2643 2697 3274 3656
Size: 53 83 186 936 1110 54 577 382
Cuts at: 3656 4291 275
Size: 635 1451
Fragments arranged by size:
1451 1110 936 635 577 382 186 83
54 53
BsaWI w'CCGG_w
Cuts at: 3900 4298 4572 4719 5130 3900
Size: 398 274 147 411 4237
Fragments arranged by size:
4237 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5242
Fragments arranged by size:
5242 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2560 1186
Size: 1374 4093
BseYI C'CCAG_C
Cuts at: 2525 2737 4670 5166 2525
Size: 212 1933 496 2826
Fragments arranged by size:
2826 1933 496 212
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2483 2600 1163
Size: 573 747 117 4030
Fragments arranged by size:
4030 747 573 117
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2917 3678 4706 1540
Size: 275 186 916 761 1028 2301
Fragments arranged by size:
2301 1028 916 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3528 3613 4684 3528
Size: 85 1071 4311
Fragments arranged by size:
4311 1071 85
BsmI GAATG_Cn'
Cuts at: 2978 3071 2978
Size: 93 5374
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2095 2179 2322 2477 2671 2786 3528
Size: 399 305 84 143 155 194 115 742
Cuts at: 3528 3613 4684 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 305 194 155 143
115 85 84
BspEI T'CCGG_A
Cuts at: 5130 5130
Size: 5467
BspHI T'CATG_A
Cuts at: 3356 5086 3356
Size: 1730 3737
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2271 2511 2565 2822 844
Size: 1427 240 54 257 3489
Fragments arranged by size:
3489 1427 257 240 54
BsrBI CCG'CTC
Cuts at: 1394 1823 3354 1394
Size: 429 1531 3507
Fragments arranged by size:
3507 1531 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3962 4136 65
Size: 1183 2714 174 1396
Fragments arranged by size:
2714 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2249 2465 2627 4111 4298 1700
Size: 110 439 216 162 1484 187 2869
Fragments arranged by size:
2869 1484 439 216 187 162 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5467
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5467
BssSI C'ACGA_G
Cuts at: 3527 4539 3527
Size: 1012 4455
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2512 2675 794
Size: 1718 163 3586
Fragments arranged by size:
3586 1718 163
BstBI TT'CG_AA
Cuts at: 4271 4271
Size: 5467
BstEII G'GTnAC_C
Cuts at: 1184 2722 1184
Size: 1538 3929
BstXI CCAn_nnnn'nTGG
Cuts at: 2707 2707
Size: 5467
BstYI r'GATC_y
Cuts at: 1972 2797 3164 3549 3566 5018 5104 5116 5462
Size: 825 367 385 17 1452 86 12 346
Cuts at: 5462 1972
Size: 1977
Fragments arranged by size:
1977 1452 825 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2045 2196 2550 513
Size: 552 939 41 151 354 3430
Fragments arranged by size:
3430 939 552 354 151 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4728
Fragments arranged by size:
4728 683 56
BtsI GCAGTG_nn'
Cuts at: 2510 2983 3777 3797 2510
Size: 473 794 20 4180
Fragments arranged by size:
4180 794 473 20
ClaI AT'CG_AT
Cuts at: 1554 2313 1554
Size: 759 4708
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5432
DraI TTT'AAA
Cuts at: 2771 2771
Size: 5467
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4474 809
Size: 507 3158 1802
Fragments arranged by size:
3158 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2199 2409 2463
Size: 54 1425 50 275 18 369 210 54
Cuts at: 2463 2629 2914 3803 5432 8
Size: 166 285 889 1629 43
Fragments arranged by size:
1629 1425 889 369 285 275 210 166
54 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2914 1537
Size: 275 1102 4090
Fragments arranged by size:
4090 1102 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2720 3397 1353
Size: 1367 677 3423
Fragments arranged by size:
3423 1367 677
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4054 4428 4574 387
Size: 1064 138 2465 374 146 1280
Fragments arranged by size:
2465 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2439 3530 4127 4914 5182 1239
Size: 393 807 1091 597 787 268 1524
Fragments arranged by size:
1524 1091 807 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2267 2267
Size: 5467
EcoO109I rG'GnC_Cy
Cuts at: 2092 2222 2319 2371 2092
Size: 130 97 52 5188
Fragments arranged by size:
5188 130 97 52
EcoRI G'AATT_C
Cuts at: 2776 3154 2776
Size: 378 5089
FspI TGC'GCA
Cuts at: 2283 3973 2283
Size: 1690 3777
HaeII r_GCGC'y
Cuts at: 1678 2638 2700 4614 1678
Size: 960 62 1914 2531
Fragments arranged by size:
2531 1914 960 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4108 4140 4182 4214 1373
Size: 32 135 32 2536 32 42 32 2626
Fragments arranged by size:
2626 2536 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2811 3057 669
Size: 988 281 873 246 3079
Fragments arranged by size:
3079 988 873 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5467
HpaI GTT'AAC
Cuts at: 3057 3057
Size: 5467
KasI G'GCGC_C
Cuts at: 2696 2696
Size: 5467
KpnI G_GTAC'C
Cuts at: 2792 2792
Size: 5467
MfeI C'AATT_G
Cuts at: 3066 3066
Size: 5467
MluI A'CGCG_T
Cuts at: 5343 5343
Size: 5467
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2742 4581 4765 1305
Size: 1437 1839 184 2007
Fragments arranged by size:
2007 1839 1437 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2411 10
Size: 54 1425 343 579 3066
Fragments arranged by size:
3066 1425 579 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2622 3425 3784 3943 518
Size: 648 1456 803 359 159 2042
Fragments arranged by size:
2042 1456 803 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2198 2287 2883 3560 4708 4953 1606
Size: 592 89 596 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 596 592 245 89
NaeI GCC'GGC
Cuts at: 2251 2467 2629 2251
Size: 216 162 5089
Fragments arranged by size:
5089 216 162
NarI GG'CG_CC
Cuts at: 2697 2697
Size: 5467
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3976
Fragments arranged by size:
3976 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5467
NgoMIV G'CCGG_C
Cuts at: 2249 2465 2627 2249
Size: 216 162 5089
Fragments arranged by size:
5089 216 162
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5467
NotI GC'GGCC_GC
Cuts at: 2914 2914
Size: 5467
NspI r_CATG'y
Cuts at: 1132 1229 2278 2825 4370 1132
Size: 97 1049 547 1545 2229
Fragments arranged by size:
2229 1545 1049 547 97
PasI CC'CwG_GG
Cuts at: 1348 2160 2692 1348
Size: 812 532 4123
Fragments arranged by size:
4123 812 532
PciI A'CATG_T
Cuts at: 4366 4366
Size: 5467
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2638 1108
Size: 1530 3937
PmeI GTTT'AAAC
Cuts at: 2771 2771
Size: 5467
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3536 3568 5049 5081 3536
Size: 32 1481 32 3922
Fragments arranged by size:
3922 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2222 2222
Size: 5467
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5467
PsiI TTA'TAA
Cuts at: 3037 3037
Size: 5467
PspOMI G'GGCC_C
Cuts at: 2091 2318 2091
Size: 227 5240
PstI C_TGCA'G
Cuts at: 830 2508 2819 830
Size: 1678 311 3478
Fragments arranged by size:
3478 1678 311
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5467
PvuII CAG'CTG
Cuts at: 2287 2287
Size: 5467
SacII CC_GC'GG
Cuts at: 2199 2199
Size: 5467
SalI G'TCGA_C
Cuts at: 1936 2809 1936
Size: 873 4594
SanDI GG'GwC_CC
Cuts at: 2222 2222
Size: 5467
SbfI CC_TGCA'GG
Cuts at: 2819 2819
Size: 5467
ScaI AGT'ACT
Cuts at: 3715 5427 3715
Size: 1712 3755
SfcI C'TryA_G
Cuts at: 826 1045 2504 2815 3950 4631 4822 826
Size: 219 1459 311 1135 681 191 1471
Fragments arranged by size:
1471 1459 1135 681 311 219 191
SfoI GGC'GCC
Cuts at: 2698 2698
Size: 5467
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5467
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5467
SmaI CCC'GGG
Cuts at: 1872 2794 1872
Size: 922 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3571 4472 4734 820
Size: 197 926 1628 901 262 1553
Fragments arranged by size:
1628 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3258 493
Size: 2765 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5467
SphI G_CATG'C
Cuts at: 1132 2825 1132
Size: 1693 3774
SspI AAT'ATT
Cuts at: 5 52 3391 5
Size: 47 3339 2081
Fragments arranged by size:
3339 2081 47
StuI AGG'CCT
Cuts at: 1517 2346 1517
Size: 829 4638
StyI C'CwwG_G
Cuts at: 513 1065 2004 2087 2412 2906 513
Size: 552 939 83 325 494 3074
Fragments arranged by size:
3074 939 552 494 325 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3494 3511 3664 3849 4294 325
Size: 1255 1914 17 153 185 445 1498
Fragments arranged by size:
1914 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3713 5425 96
Size: 274 80 33 51 1443 1736 1712 138
Fragments arranged by size:
1736 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5427 5459 5427
Size: 32 5435
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5467
XbaI T'CTAG_A
Cuts at: 2803 2803
Size: 5467
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5467
XmaI C'CCGG_G
Cuts at: 1870 2792 1870
Size: 922 4545
XmnI GAAnn'nnTTC
Cuts at: 3596 3596
Size: 5467
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3275 276
Size: 53 83 186 936 1741 2468
Fragments arranged by size:
2468 1741 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SacI SapI SexAI SfiI SrfI SwaI
XcmI