Restriction map for
pFN21AE1749
(Circular) (Six-base) MAPSORT of: pFN21AE1749.seq Check: 7884 from: 1 to: 5461
pFN21AE1749 5461 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3271 278
Size: 53 83 186 936 1735 2468
Fragments arranged by size:
2468 1735 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2804 1937
Size: 867 4594
Acc65I G'GTAC_C
Cuts at: 2782 2782
Size: 5461
AclI AA'CG_TT
Cuts at: 1563 3588 3961 1563
Size: 2025 373 3063
Fragments arranged by size:
3063 2025 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2040 2268 2750 3524 4908 5176 1632
Size: 408 228 482 774 1384 268 1917
Fragments arranged by size:
1917 1384 774 482 408 268 228
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5264
AflIII A'CryG_T
Cuts at: 1203 4360 5337 1203
Size: 3157 977 1327
Fragments arranged by size:
3157 1327 977
AgeI A'CCGG_T
Cuts at: 4292 4292
Size: 5461
AhdI GACnn_n'nnGTC
Cuts at: 4190 4190
Size: 5461
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5461
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5461
AlwNI CAG_nnn'CTG
Cuts at: 2134 2291 3204 4776 2134
Size: 157 913 1572 2819
Fragments arranged by size:
2819 1572 913 157
ApaLI G'TGCA_C
Cuts at: 2562 3518 4674 2562
Size: 956 1156 3349
Fragments arranged by size:
3349 1156 956
ApoI r'AATT_y
Cuts at: 1493 2770 2996 3148 5257 1493
Size: 1277 226 152 2109 1697
Fragments arranged by size:
2109 1697 1277 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4015 160
Size: 872 2983 1606
Fragments arranged by size:
2983 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5461
AvaI C'yCGr_G
Cuts at: 1870 1943 2786 1870
Size: 73 843 4545
Fragments arranged by size:
4545 843 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2134 2167 2134
Size: 33 5428
BamHI G'GATC_C
Cuts at: 2477 2791 3158 2477
Size: 314 367 4780
Fragments arranged by size:
4780 367 314
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2386 2782 4237 618
Size: 325 217 627 599 396 1455 1842
Fragments arranged by size:
1842 1455 627 599 396 325 217
BanII G_rGCy'C
Cuts at: 1391 2780 1391
Size: 1389 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2186 2508 928
Size: 971 287 322 3881
Fragments arranged by size:
3881 971 322 287
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2089 2123 3652 3686 1903
Size: 34 152 34 1529 34 3678
Fragments arranged by size:
3678 1529 152 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3353 4563 3353
Size: 1210 4251
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5461
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2238 4072 136
Size: 107 122 71 631 605 566 1834 1525
Fragments arranged by size:
1834 1525 631 605 566 122 107 71
BglII A'GATC_T
Cuts at: 5456 5456
Size: 5461
BlpI GC'TnA_GC
Cuts at: 2878 2878
Size: 5461
Bme1580I G_kGCm'C
Cuts at: 1790 2389 2566 3522 4678 1790
Size: 599 177 956 1156 2573
Fragments arranged by size:
2573 1156 956 599 177
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5461
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3192 4150 442
Size: 416 53 83 399 24 1775 958 1753
Fragments arranged by size:
1775 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5461
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2070 2744 4121 1239
Size: 831 674 1377 2579
Fragments arranged by size:
2579 1377 831 674
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2327 3586 4451 4749 2327
Size: 1259 865 298 3039
Fragments arranged by size:
3039 1259 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4124 882
Size: 617 2625 2219
Fragments arranged by size:
2625 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3252 493
Size: 711 2048 2702
Fragments arranged by size:
2702 2048 711
BsaBI GATnn'nnATC
Cuts at: 5455 5455
Size: 5461
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3268 3650 4285 275
Size: 53 83 186 936 1735 382 635 1451
Fragments arranged by size:
1735 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3894 4292 4566 4713 5124 3894
Size: 398 274 147 411 4231
Fragments arranged by size:
4231 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2350 2380 1178
Size: 30 165 30 947 30 4259
Fragments arranged by size:
4259 947 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2720 1186
Size: 1534 3927
BseYI C'CCAG_C
Cuts at: 4664 5160 4664
Size: 496 4965
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2546 1163
Size: 573 810 4078
Fragments arranged by size:
4078 810 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2911 3672 4700 1540
Size: 275 186 910 761 1028 2301
Fragments arranged by size:
2301 1028 910 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2070 2566 3522 3607 4678 2070
Size: 496 956 85 1071 2853
Fragments arranged by size:
2853 1071 956 496 85
BsmI GAATG_Cn'
Cuts at: 2972 3065 2972
Size: 93 5368
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2389 2566 2780 3522 3607 4678
Size: 399 280 319 177 214 742 85 1071
Cuts at: 4678 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 319 280 214 177
85
BspEI T'CCGG_A
Cuts at: 5124 5124
Size: 5461
BspHI T'CATG_A
Cuts at: 3350 5080 3350
Size: 1730 3731
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2816 844
Size: 1972 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3348 1394
Size: 429 1525 3507
Fragments arranged by size:
3507 1525 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2200 2332 2543 3956 4130 65
Size: 1183 952 132 211 1413 174 1396
Fragments arranged by size:
1413 1396 1183 952 211 174 132
BsrFI r'CCGG_y
Cuts at: 1700 1810 4105 4292 1700
Size: 110 2295 187 2869
Fragments arranged by size:
2869 2295 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5461
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5461
BssSI C'ACGA_G
Cuts at: 3521 4533 3521
Size: 1012 4449
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2624 794
Size: 1830 3631
BstBI TT'CG_AA
Cuts at: 4265 4265
Size: 5461
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5461
BstYI r'GATC_y
Cuts at: 1972 2477 2791 3158 3543 3560 5012 5098 5110
Size: 505 314 367 385 17 1452 86 12
Cuts at: 5110 5456 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 505 385 367 346 314 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2473 2473
Size: 5461
BtgI C'CryG_G
Cuts at: 513 1065 2658 513
Size: 552 1593 3316
Fragments arranged by size:
3316 1593 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4722
Fragments arranged by size:
4722 683 56
BtsI GCAGTG_nn'
Cuts at: 2566 2977 3771 3791 2566
Size: 411 794 20 4236
Fragments arranged by size:
4236 794 411 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5461
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2100 2135 529
Size: 35 1536 35 3855
Fragments arranged by size:
3855 1536 35 35
DraI TTT'AAA
Cuts at: 2765 2765
Size: 5461
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4468 809
Size: 507 3152 1802
Fragments arranged by size:
3152 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2908 3797 5426
Size: 54 1425 50 275 18 1078 889 1629
Cuts at: 5426 8
Size: 43
Fragments arranged by size:
1629 1425 1078 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2908 1537
Size: 275 1096 4090
Fragments arranged by size:
4090 1096 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3391 1353
Size: 2038 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4048 4422 4568 387
Size: 1064 138 2459 374 146 1280
Fragments arranged by size:
2459 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2040 2070 2268 2744 2750 3524 4121
Size: 393 408 30 198 476 6 774 597
Cuts at: 4121 4908 5176 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 774 597 476 408 393 268
198 30 6
EcoO109I rG'GnC_Cy
Cuts at: 2288 2288
Size: 5461
EcoRI G'AATT_C
Cuts at: 2770 3148 2770
Size: 378 5083
FspI TGC'GCA
Cuts at: 3967 3967
Size: 5461
HaeII r_GCGC'y
Cuts at: 1678 4608 1678
Size: 2930 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2350 2382 4102 4134 4176
Size: 32 135 32 778 32 1720 32 42
Cuts at: 4176 4208 1373
Size: 32 2626
Fragments arranged by size:
2626 1720 778 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2805 3051 669
Size: 988 281 867 246 3079
Fragments arranged by size:
3079 988 867 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5461
HpaI GTT'AAC
Cuts at: 3051 3051
Size: 5461
KpnI G_GTAC'C
Cuts at: 2786 2786
Size: 5461
MfeI C'AATT_G
Cuts at: 3060 3060
Size: 5461
MluI A'CGCG_T
Cuts at: 5337 5337
Size: 5461
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4575 4759 1305
Size: 3270 184 2007
Fragments arranged by size:
3270 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3639
Fragments arranged by size:
3639 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2318 2699 3419 3778 3937 518
Size: 648 1152 381 720 359 159 2042
Fragments arranged by size:
2042 1152 720 648 381 359 159
MspA1I CmG'CkG
Cuts at: 1606 2877 3554 4702 4947 1606
Size: 1271 677 1148 245 2120
Fragments arranged by size:
2120 1271 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2658 513
Size: 552 1593 3316
Fragments arranged by size:
3316 1593 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5461
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5461
NotI GC'GGCC_GC
Cuts at: 2908 2908
Size: 5461
NspI r_CATG'y
Cuts at: 1132 1229 2819 4364 1132
Size: 97 1590 1545 2229
Fragments arranged by size:
2229 1590 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5461
PciI A'CATG_T
Cuts at: 4360 4360
Size: 5461
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2444 1108
Size: 1336 4125
PfoI T'CCnGG_A
Cuts at: 2586 2586
Size: 5461
PmeI GTTT'AAAC
Cuts at: 2765 2765
Size: 5461
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3530 3562 5043 5075 3530
Size: 32 1481 32 3916
Fragments arranged by size:
3916 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5461
PsiI TTA'TAA
Cuts at: 3031 3031
Size: 5461
PstI C_TGCA'G
Cuts at: 830 2813 830
Size: 1983 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5461
SalI G'TCGA_C
Cuts at: 1936 2803 1936
Size: 867 4594
SbfI CC_TGCA'GG
Cuts at: 2813 2813
Size: 5461
ScaI AGT'ACT
Cuts at: 3709 5421 3709
Size: 1712 3749
SexAI A'CCwGG_T
Cuts at: 2166 2166
Size: 5461
SfcI C'TryA_G
Cuts at: 826 1045 2809 3944 4625 4816 826
Size: 219 1764 1135 681 191 1471
Fragments arranged by size:
1764 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5461
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5461
SmaI CCC'GGG
Cuts at: 1872 2788 1872
Size: 916 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2342 3565 4466 4728 820
Size: 197 926 399 1223 901 262 1553
Fragments arranged by size:
1553 1223 926 901 399 262 197
SnaBI TAC'GTA
Cuts at: 493 3252 493
Size: 2759 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5461
SphI G_CATG'C
Cuts at: 1132 2819 1132
Size: 1687 3774
SspI AAT'ATT
Cuts at: 5 52 2717 3385 5
Size: 47 2665 668 2081
Fragments arranged by size:
2665 2081 668 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5461
StyI C'CwwG_G
Cuts at: 513 1065 2658 2900 513
Size: 552 1593 242 3074
Fragments arranged by size:
3074 1593 552 242
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3488 3505 3658 3843 4288 325
Size: 1255 1908 17 153 185 445 1498
Fragments arranged by size:
1908 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3707 5419 96
Size: 274 80 33 51 1443 1730 1712 138
Fragments arranged by size:
1730 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5421 5453 5421
Size: 32 5429
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5461
XbaI T'CTAG_A
Cuts at: 2368 2797 2368
Size: 429 5032
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5461
XmaI C'CCGG_G
Cuts at: 1870 2786 1870
Size: 916 4545
XmnI GAAnn'nnTTC
Cuts at: 3590 3590
Size: 5461
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3269 276
Size: 53 83 186 936 1735 2468
Fragments arranged by size:
2468 1735 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI XcmI