Restriction map for
pFN21AE1742
(Circular) (Six-base) MAPSORT of: pFN21AE1742.seq Check: 4962 from: 1 to: 5437
pFN21AE1742 5437 bp
With 182 enzymes: SgfI *
August 16, 2021 15:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2361 2361
Size: 5437
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3247 278
Size: 53 83 186 936 1711 2468
Fragments arranged by size:
2468 1711 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2780 1937
Size: 843 4594
Acc65I G'GTAC_C
Cuts at: 2758 2758
Size: 5437
AclI AA'CG_TT
Cuts at: 1563 3564 3937 1563
Size: 2001 373 3063
Fragments arranged by size:
3063 2001 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2164 2684 3500 4884 5152 1632
Size: 532 520 816 1384 268 1917
Fragments arranged by size:
1917 1384 816 532 520 268
AfeI AGC'GCT
Cuts at: 2366 2366
Size: 5437
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5240
AflIII A'CryG_T
Cuts at: 1203 4336 5313 1203
Size: 3133 977 1327
Fragments arranged by size:
3133 1327 977
AgeI A'CCGG_T
Cuts at: 4268 4268
Size: 5437
AhdI GACnn_n'nnGTC
Cuts at: 4166 4166
Size: 5437
AleI CACnn'nnGTG
Cuts at: 1166 2275 2438 1166
Size: 1109 163 4165
Fragments arranged by size:
4165 1109 163
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2214 2456 1184
Size: 1030 242 4165
Fragments arranged by size:
4165 1030 242
AlwNI CAG_nnn'CTG
Cuts at: 3180 4752 3180
Size: 1572 3865
ApaI G_GGCC'C
Cuts at: 2207 2207
Size: 5437
ApaLI G'TGCA_C
Cuts at: 3494 4650 3494
Size: 1156 4281
ApoI r'AATT_y
Cuts at: 1493 2746 2972 3124 5233 1493
Size: 1253 226 152 2109 1697
Fragments arranged by size:
2109 1697 1253 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3991 160
Size: 872 2959 1606
Fragments arranged by size:
2959 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5437
AvaI C'yCGr_G
Cuts at: 1870 1943 2762 1870
Size: 73 819 4545
Fragments arranged by size:
4545 819 73
BamHI G'GATC_C
Cuts at: 2578 2767 3134 2578
Size: 189 367 4881
Fragments arranged by size:
4881 367 189
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2589 2758 4213 618
Size: 325 217 627 802 169 1455 1842
Fragments arranged by size:
1842 1455 802 627 325 217 169
BanII G_rGCy'C
Cuts at: 1391 2178 2207 2756 1391
Size: 787 29 549 4072
Fragments arranged by size:
4072 787 549 29
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4466
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3628 3662 1903
Size: 34 1691 34 3678
Fragments arranged by size:
3678 1691 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3329 4539 3329
Size: 1210 4227
BclI T'GATC_A
Cuts at: 1548 2325 1548
Size: 777 4660
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4048 136
Size: 107 122 71 631 605 2376 1525
Fragments arranged by size:
2376 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5432 5432
Size: 5437
BlpI GC'TnA_GC
Cuts at: 2854 2854
Size: 5437
Bme1580I G_kGCm'C
Cuts at: 1790 2207 3498 4654 1790
Size: 417 1291 1156 2573
Fragments arranged by size:
2573 1291 1156 417
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5437
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3168 4126 442
Size: 416 53 83 399 24 1751 958 1753
Fragments arranged by size:
1753 1751 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5437
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2660 4097 1239
Size: 1421 1437 2579
Fragments arranged by size:
2579 1437 1421
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3562 4427 4725 3562
Size: 865 298 4274
Fragments arranged by size:
4274 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2491 4100 882
Size: 617 992 1609 2219
Fragments arranged by size:
2219 1609 992 617
BsaAI yAC'GTr
Cuts at: 493 1204 3228 493
Size: 711 2024 2702
Fragments arranged by size:
2702 2024 711
BsaBI GATnn'nnATC
Cuts at: 5431 5431
Size: 5437
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3244 3626 4261 275
Size: 53 83 186 936 1711 382 635 1451
Fragments arranged by size:
1711 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3870 4268 4542 4689 5100 3870
Size: 398 274 147 411 4207
Fragments arranged by size:
4207 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5212
Fragments arranged by size:
5212 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5437
BseYI C'CCAG_C
Cuts at: 4640 5136 4640
Size: 496 4941
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2299 2416 2506 1163
Size: 573 563 117 90 4094
Fragments arranged by size:
4094 573 563 117 90
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2887 3648 4676 1540
Size: 275 186 886 761 1028 2301
Fragments arranged by size:
2301 1028 886 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2178 2450 3498 3583 4654 2178
Size: 272 1048 85 1071 2961
Fragments arranged by size:
2961 1071 1048 272 85
BsmI GAATG_Cn'
Cuts at: 2948 3041 2948
Size: 93 5344
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2178 2207 2450 2756 3498 3583 4654
Size: 399 388 29 243 306 742 85 1071
Cuts at: 4654 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 388 306 243 85
29
BspEI T'CCGG_A
Cuts at: 5100 5100
Size: 5437
BspHI T'CATG_A
Cuts at: 3326 5056 3326
Size: 1730 3707
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2073 2196 2361 2792 844
Size: 1229 123 165 431 3489
Fragments arranged by size:
3489 1229 431 165 123
BsrBI CCG'CTC
Cuts at: 1394 1823 2467 2644 3324 1394
Size: 429 644 177 680 3507
Fragments arranged by size:
3507 680 644 429 177
BsrDI GCAATG_nn'
Cuts at: 65 1248 2334 3932 4106 65
Size: 1183 1086 1598 174 1396
Fragments arranged by size:
1598 1396 1183 1086 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4081 4268 1700
Size: 110 2271 187 2869
Fragments arranged by size:
2869 2271 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5437
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5437
BssSI C'ACGA_G
Cuts at: 3497 4509 3497
Size: 1012 4425
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5437
BstBI TT'CG_AA
Cuts at: 4241 4241
Size: 5437
BstEII G'GTnAC_C
Cuts at: 1184 2284 1184
Size: 1100 4337
BstXI CCAn_nnnn'nTGG
Cuts at: 2694 2694
Size: 5437
BstYI r'GATC_y
Cuts at: 1972 2578 2767 3134 3519 3536 4988 5074 5086
Size: 606 189 367 385 17 1452 86 12
Cuts at: 5086 5432 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 606 385 367 346 189 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3946
Fragments arranged by size:
3946 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4698
Fragments arranged by size:
4698 683 56
BtsI GCAGTG_nn'
Cuts at: 2953 3747 3767 2953
Size: 794 20 4623
Fragments arranged by size:
4623 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5437
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5402
DraI TTT'AAA
Cuts at: 2741 2741
Size: 5437
DraIII CAC_nnn'GTG
Cuts at: 2401 2401
Size: 5437
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4444 809
Size: 507 3128 1802
Fragments arranged by size:
3128 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2884 3773
Size: 54 1425 50 275 18 204 850 889
Cuts at: 3773 5402 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 850 275 204 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2884 1537
Size: 275 1072 4090
Fragments arranged by size:
4090 1072 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2183 3367 1353
Size: 830 1184 3423
Fragments arranged by size:
3423 1184 830
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4024 4398 4544 387
Size: 1064 138 2435 374 146 1280
Fragments arranged by size:
2435 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2176 2176
Size: 5437
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2164 2660 2684 3500 4097 4884 5152
Size: 393 532 496 24 816 597 787 268
Cuts at: 5152 1239
Size: 1524
Fragments arranged by size:
1524 816 787 597 532 496 393 268
24
EcoO109I rG'GnC_Cy
Cuts at: 2204 2635 2683 2204
Size: 431 48 4958
Fragments arranged by size:
4958 431 48
EcoRI G'AATT_C
Cuts at: 2746 3124 2746
Size: 378 5059
FspI TGC'GCA
Cuts at: 3943 3943
Size: 5437
HaeII r_GCGC'y
Cuts at: 1678 2368 4584 1678
Size: 690 2216 2531
Fragments arranged by size:
2531 2216 690
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4078 4110 4152 4184 1373
Size: 32 135 32 2506 32 42 32 2626
Fragments arranged by size:
2626 2506 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2781 3027 669
Size: 988 281 843 246 3079
Fragments arranged by size:
3079 988 843 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5437
HpaI GTT'AAC
Cuts at: 3027 3027
Size: 5437
KpnI G_GTAC'C
Cuts at: 2762 2762
Size: 5437
MfeI C'AATT_G
Cuts at: 3036 3036
Size: 5437
MluI A'CGCG_T
Cuts at: 5313 5313
Size: 5437
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4551 4735 1305
Size: 3246 184 2007
Fragments arranged by size:
3246 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2036 10
Size: 54 1425 343 204 3411
Fragments arranged by size:
3411 1425 343 204 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2275 2438 2718 3395 3754 3913 518
Size: 648 1109 163 280 677 359 159 2042
Fragments arranged by size:
2042 1109 677 648 359 280 163 159
MspA1I CmG'CkG
Cuts at: 1606 2537 2853 3530 4678 4923 1606
Size: 931 316 677 1148 245 2120
Fragments arranged by size:
2120 1148 931 677 316 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3946
Fragments arranged by size:
3946 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5437
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5437
NotI GC'GGCC_GC
Cuts at: 2884 2884
Size: 5437
NspI r_CATG'y
Cuts at: 1132 1229 2395 2531 2795 4340 1132
Size: 97 1166 136 264 1545 2229
Fragments arranged by size:
2229 1545 1166 264 136 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5437
PciI A'CATG_T
Cuts at: 4336 4336
Size: 5437
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5437
PfoI T'CCnGG_A
Cuts at: 2210 2574 2210
Size: 364 5073
PmeI GTTT'AAAC
Cuts at: 2741 2741
Size: 5437
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3506 3538 5019 5051 3506
Size: 32 1481 32 3892
Fragments arranged by size:
3892 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5437
PsiI TTA'TAA
Cuts at: 3007 3007
Size: 5437
PspOMI G'GGCC_C
Cuts at: 2203 2203
Size: 5437
PstI C_TGCA'G
Cuts at: 830 2193 2358 2673 2789 830
Size: 1363 165 315 116 3478
Fragments arranged by size:
3478 1363 315 165 116
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5437
PvuII CAG'CTG
Cuts at: 2537 2537
Size: 5437
SacI G_AGCT'C
Cuts at: 2178 2178
Size: 5437
SalI G'TCGA_C
Cuts at: 1936 2779 1936
Size: 843 4594
SapI GCTCTTCn'nnn_
Cuts at: 2183 2183
Size: 5437
SbfI CC_TGCA'GG
Cuts at: 2193 2789 2193
Size: 596 4841
ScaI AGT'ACT
Cuts at: 3685 5397 3685
Size: 1712 3725
SexAI A'CCwGG_T
Cuts at: 2397 2397
Size: 5437
SfcI C'TryA_G
Cuts at: 826 1045 2189 2354 2669 2785 3920 4601 4792
Size: 219 1144 165 315 116 1135 681 191
Cuts at: 4792 826
Size: 1471
Fragments arranged by size:
1471 1144 1135 681 315 219 191 165
116
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5437
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5437
SmaI CCC'GGG
Cuts at: 1872 2764 1872
Size: 892 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3541 4442 4704 820
Size: 197 926 1598 901 262 1553
Fragments arranged by size:
1598 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3228 493
Size: 2735 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5437
SphI G_CATG'C
Cuts at: 1132 2795 1132
Size: 1663 3774
SspI AAT'ATT
Cuts at: 5 52 3361 5
Size: 47 3309 2081
Fragments arranged by size:
3309 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5437
StyI C'CwwG_G
Cuts at: 513 1065 2004 2876 513
Size: 552 939 872 3074
Fragments arranged by size:
3074 939 872 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2265 2595 3464 3481 3634 3819 4264
Size: 1255 685 330 869 17 153 185 445
Cuts at: 4264 325
Size: 1498
Fragments arranged by size:
1498 1255 869 685 445 330 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3683 5395 96
Size: 274 80 33 51 1443 1706 1712 138
Fragments arranged by size:
1712 1706 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2715 2747 5397 5429 2715
Size: 32 2650 32 2723
Fragments arranged by size:
2723 2650 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5437
XbaI T'CTAG_A
Cuts at: 2773 2773
Size: 5437
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2405 2405
Size: 5437
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5437
XmaI C'CCGG_G
Cuts at: 1870 2762 1870
Size: 892 4545
XmnI GAAnn'nnTTC
Cuts at: 2558 3566 2558
Size: 1008 4429
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3245 276
Size: 53 83 186 936 1711 2468
Fragments arranged by size:
2468 1711 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI
SfiI SfoI SrfI SwaI