Restriction map for
pFN21AE1741
(Circular) (Six-base) MAPSORT of: pFN21AE1741.seq Check: 2357 from: 1 to: 5437
pFN21AE1741 5437 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3247 278
Size: 53 83 186 936 1711 2468
Fragments arranged by size:
2468 1711 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2780 1937
Size: 843 4594
Acc65I G'GTAC_C
Cuts at: 2758 2758
Size: 5437
AclI AA'CG_TT
Cuts at: 1563 3564 3937 1563
Size: 2001 373 3063
Fragments arranged by size:
3063 2001 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3500 4884 5152 1632
Size: 1868 1384 268 1917
Fragments arranged by size:
1917 1868 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5240
AflIII A'CryG_T
Cuts at: 1203 4336 5313 1203
Size: 3133 977 1327
Fragments arranged by size:
3133 1327 977
AgeI A'CCGG_T
Cuts at: 4268 4268
Size: 5437
AhdI GACnn_n'nnGTC
Cuts at: 4166 4166
Size: 5437
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5437
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2268 1184
Size: 1084 4353
AlwNI CAG_nnn'CTG
Cuts at: 2694 3180 4752 2694
Size: 486 1572 3379
Fragments arranged by size:
3379 1572 486
ApaLI G'TGCA_C
Cuts at: 3494 4650 3494
Size: 1156 4281
ApoI r'AATT_y
Cuts at: 1493 2185 2746 2972 3124 5233 1493
Size: 692 561 226 152 2109 1697
Fragments arranged by size:
2109 1697 692 561 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3991 160
Size: 872 2959 1606
Fragments arranged by size:
2959 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5437
AvaI C'yCGr_G
Cuts at: 1870 1943 2762 1870
Size: 73 819 4545
Fragments arranged by size:
4545 819 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2398 2431 2398
Size: 33 5404
BamHI G'GATC_C
Cuts at: 2767 3134 2767
Size: 367 5070
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2758 4213 618
Size: 325 217 627 971 1455 1842
Fragments arranged by size:
1842 1455 971 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2756 1391
Size: 1365 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4466
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3628 3662 1903
Size: 34 1691 34 3678
Fragments arranged by size:
3678 1691 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2399 3329 4539 2399
Size: 930 1210 3297
Fragments arranged by size:
3297 1210 930
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5437
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4048 136
Size: 107 122 71 631 605 2376 1525
Fragments arranged by size:
2376 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5432 5432
Size: 5437
BlpI GC'TnA_GC
Cuts at: 2656 2854 2656
Size: 198 5239
Bme1580I G_kGCm'C
Cuts at: 1790 3498 4654 1790
Size: 1708 1156 2573
Fragments arranged by size:
2573 1708 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5437
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2346 3168 4126
Size: 416 53 83 399 24 929 822 958
Cuts at: 4126 442
Size: 1753
Fragments arranged by size:
1753 958 929 822 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5437
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2357 2517 2716 4097 1239
Size: 1118 160 199 1381 2579
Fragments arranged by size:
2579 1381 1118 199 160
Bpu10I CC'TnA_GC
Cuts at: 2588 2588
Size: 5437
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3562 4427 4725 3562
Size: 865 298 4274
Fragments arranged by size:
4274 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2566 4100 882
Size: 617 1067 1534 2219
Fragments arranged by size:
2219 1534 1067 617
BsaAI yAC'GTr
Cuts at: 493 1204 3228 493
Size: 711 2024 2702
Fragments arranged by size:
2702 2024 711
BsaBI GATnn'nnATC
Cuts at: 5431 5431
Size: 5437
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3244 3626 4261 275
Size: 53 83 186 936 1711 382 635 1451
Fragments arranged by size:
1711 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3870 4268 4542 4689 5100 3870
Size: 398 274 147 411 4207
Fragments arranged by size:
4207 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5212
Fragments arranged by size:
5212 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2444 1186
Size: 1258 4179
BseYI C'CCAG_C
Cuts at: 4640 5136 4640
Size: 496 4941
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4864
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2016 2887 3648 4676 1540
Size: 275 186 15 871 761 1028 2301
Fragments arranged by size:
2301 1028 871 761 275 186 15
BsiHKAI G_wGCw'C
Cuts at: 3498 3583 4654 3498
Size: 85 1071 4281
Fragments arranged by size:
4281 1071 85
BsmI GAATG_Cn'
Cuts at: 2948 3041 2948
Size: 93 5344
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2756 3498 3583 4654 1391
Size: 399 966 742 85 1071 2174
Fragments arranged by size:
2174 1071 966 742 399 85
BspEI T'CCGG_A
Cuts at: 5100 5100
Size: 5437
BspHI T'CATG_A
Cuts at: 3326 5056 3326
Size: 1730 3707
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2067 2362 2792 844
Size: 1223 295 430 3489
Fragments arranged by size:
3489 1223 430 295
BsrBI CCG'CTC
Cuts at: 1394 1823 3324 1394
Size: 429 1501 3507
Fragments arranged by size:
3507 1501 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3932 4106 65
Size: 1183 2684 174 1396
Fragments arranged by size:
2684 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4081 4268 1700
Size: 110 2271 187 2869
Fragments arranged by size:
2869 2271 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5437
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5437
BssSI C'ACGA_G
Cuts at: 3497 4509 3497
Size: 1012 4425
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5437
BstBI TT'CG_AA
Cuts at: 4241 4241
Size: 5437
BstEII G'GTnAC_C
Cuts at: 1184 2231 1184
Size: 1047 4390
BstYI r'GATC_y
Cuts at: 1972 2417 2767 3134 3519 3536 4988 5074 5086
Size: 445 350 367 385 17 1452 86 12
Cuts at: 5086 5432 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 445 385 367 350 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2413 2643 513
Size: 552 939 409 230 3307
Fragments arranged by size:
3307 939 552 409 230
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4698
Fragments arranged by size:
4698 683 56
BtsI GCAGTG_nn'
Cuts at: 2215 2953 3747 3767 2215
Size: 738 794 20 3885
Fragments arranged by size:
3885 794 738 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5437
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5402
DraI TTT'AAA
Cuts at: 2741 2741
Size: 5437
DraIII CAC_nnn'GTG
Cuts at: 2354 2354
Size: 5437
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4444 809
Size: 507 3128 1802
Fragments arranged by size:
3128 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2884 3773
Size: 54 1425 50 275 18 183 871 889
Cuts at: 3773 5402 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 871 275 183 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2013 2884 1537
Size: 275 201 871 4090
Fragments arranged by size:
4090 871 275 201
EarI CTCTTCn'nnn_
Cuts at: 1353 2235 2609 3367 1353
Size: 882 374 758 3423
Fragments arranged by size:
3423 882 758 374
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2521 4024 4398 4544 387
Size: 1064 138 932 1503 374 146 1280
Fragments arranged by size:
1503 1280 1064 932 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2357 2517 2716 3500 4097 4884 5152
Size: 393 725 160 199 784 597 787 268
Cuts at: 5152 1239
Size: 1524
Fragments arranged by size:
1524 787 784 725 597 393 268 199
160
EcoRI G'AATT_C
Cuts at: 2746 3124 2746
Size: 378 5059
FspI TGC'GCA
Cuts at: 3943 3943
Size: 5437
HaeII r_GCGC'y
Cuts at: 1678 4584 1678
Size: 2906 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2084 2116 4078 4110 4152
Size: 32 135 32 512 32 1962 32 42
Cuts at: 4152 4184 1373
Size: 32 2626
Fragments arranged by size:
2626 1962 512 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2781 3027 669
Size: 988 281 843 246 3079
Fragments arranged by size:
3079 988 843 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5437
HpaI GTT'AAC
Cuts at: 3027 3027
Size: 5437
KpnI G_GTAC'C
Cuts at: 2762 2762
Size: 5437
MfeI C'AATT_G
Cuts at: 3036 3036
Size: 5437
MluI A'CGCG_T
Cuts at: 5313 5313
Size: 5437
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4551 4735 1305
Size: 3246 184 2007
Fragments arranged by size:
3246 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3615
Fragments arranged by size:
3615 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3395 3754 3913 518
Size: 648 2229 359 159 2042
Fragments arranged by size:
2229 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2012 2248 2853 3530 4678 4923 1606
Size: 406 236 605 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 605 406 245 236
NcoI C'CATG_G
Cuts at: 513 1065 2004 2643 513
Size: 552 939 639 3307
Fragments arranged by size:
3307 939 639 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5437
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5437
NotI GC'GGCC_GC
Cuts at: 2013 2884 2013
Size: 871 4566
NruI TCG'CGA
Cuts at: 2045 2045
Size: 5437
NspI r_CATG'y
Cuts at: 1132 1229 2795 4340 1132
Size: 97 1566 1545 2229
Fragments arranged by size:
2229 1566 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5437
PciI A'CATG_T
Cuts at: 4336 4336
Size: 5437
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5437
PfoI T'CCnGG_A
Cuts at: 2075 2075
Size: 5437
PmeI GTTT'AAAC
Cuts at: 2741 2741
Size: 5437
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3506 3538 5019 5051 3506
Size: 32 1481 32 3892
Fragments arranged by size:
3892 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5437
PsiI TTA'TAA
Cuts at: 3007 3007
Size: 5437
PstI C_TGCA'G
Cuts at: 830 2373 2789 830
Size: 1543 416 3478
Fragments arranged by size:
3478 1543 416
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5437
PvuII CAG'CTG
Cuts at: 2248 2248
Size: 5437
SalI G'TCGA_C
Cuts at: 1936 2779 1936
Size: 843 4594
SapI GCTCTTCn'nnn_
Cuts at: 2235 2235
Size: 5437
SbfI CC_TGCA'GG
Cuts at: 2789 2789
Size: 5437
ScaI AGT'ACT
Cuts at: 3685 5397 3685
Size: 1712 3725
SfcI C'TryA_G
Cuts at: 826 1045 2369 2785 3920 4601 4792 826
Size: 219 1324 416 1135 681 191 1471
Fragments arranged by size:
1471 1324 1135 681 416 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5437
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5437
SmaI CCC'GGG
Cuts at: 1872 2764 1872
Size: 892 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3541 4442 4704 820
Size: 197 926 1598 901 262 1553
Fragments arranged by size:
1598 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3228 493
Size: 2735 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5437
SphI G_CATG'C
Cuts at: 1132 2795 1132
Size: 1663 3774
SspI AAT'ATT
Cuts at: 5 52 3361 5
Size: 47 3309 2081
Fragments arranged by size:
3309 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5437
StyI C'CwwG_G
Cuts at: 513 1065 2004 2643 2876 513
Size: 552 939 639 233 3074
Fragments arranged by size:
3074 939 639 552 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2365 2653 3464 3481 3634 3819 4264
Size: 1255 785 288 811 17 153 185 445
Cuts at: 4264 325
Size: 1498
Fragments arranged by size:
1498 1255 811 785 445 288 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3683 5395 96
Size: 274 80 33 51 1443 1706 1712 138
Fragments arranged by size:
1712 1706 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2371 2403 2674 2706 5397 5429 2371
Size: 32 271 32 2691 32 2379
Fragments arranged by size:
2691 2379 271 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2216 1316
Size: 900 4537
XbaI T'CTAG_A
Cuts at: 2773 2773
Size: 5437
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5437
XmaI C'CCGG_G
Cuts at: 1870 2762 1870
Size: 892 4545
XmnI GAAnn'nnTTC
Cuts at: 3566 3566
Size: 5437
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3245 276
Size: 53 83 186 936 1711 2468
Fragments arranged by size:
2468 1711 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NruI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI