Restriction map for
pFN21AE1739
(Circular) (Six-base) MAPSORT of: pFN21AE1739.seq Check: 1305 from: 1 to: 5437
pFN21AE1739 5437 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3247 278
Size: 53 83 186 936 1711 2468
Fragments arranged by size:
2468 1711 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2780 1937
Size: 843 4594
Acc65I G'GTAC_C
Cuts at: 2758 2758
Size: 5437
AclI AA'CG_TT
Cuts at: 1563 3564 3937 1563
Size: 2001 373 3063
Fragments arranged by size:
3063 2001 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2315 3500 4884 5152 1632
Size: 683 1185 1384 268 1917
Fragments arranged by size:
1917 1384 1185 683 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5240
AflIII A'CryG_T
Cuts at: 1203 4336 5313 1203
Size: 3133 977 1327
Fragments arranged by size:
3133 1327 977
AgeI A'CCGG_T
Cuts at: 4268 4268
Size: 5437
AhdI GACnn_n'nnGTC
Cuts at: 4166 4166
Size: 5437
AleI CACnn'nnGTG
Cuts at: 1166 2615 1166
Size: 1449 3988
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5437
AlwNI CAG_nnn'CTG
Cuts at: 2224 3180 4752 2224
Size: 956 1572 2909
Fragments arranged by size:
2909 1572 956
ApaLI G'TGCA_C
Cuts at: 3494 4650 3494
Size: 1156 4281
ApoI r'AATT_y
Cuts at: 1493 2746 2972 3124 5233 1493
Size: 1253 226 152 2109 1697
Fragments arranged by size:
2109 1697 1253 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3991 160
Size: 872 2959 1606
Fragments arranged by size:
2959 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5437
AvaI C'yCGr_G
Cuts at: 1870 1943 2186 2258 2528 2540 2762 1870
Size: 73 243 72 270 12 222 4545
Fragments arranged by size:
4545 270 243 222 73 72 12
BamHI G'GATC_C
Cuts at: 2767 3134 2767
Size: 367 5070
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2110 2437 2507 2546 2758
Size: 325 217 627 323 327 70 39 212
Cuts at: 2758 4213 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 327 325 323 217 212
70 39
BanII G_rGCy'C
Cuts at: 1391 2265 2756 1391
Size: 874 491 4072
Fragments arranged by size:
4072 874 491
BbeI G_GCGC'C
Cuts at: 2441 2511 2550 2441
Size: 70 39 5328
Fragments arranged by size:
5328 70 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2714 928
Size: 971 815 3651
Fragments arranged by size:
3651 971 815
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3628 3662 1903
Size: 34 1691 34 3678
Fragments arranged by size:
3678 1691 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3329 4539 3329
Size: 1210 4227
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5437
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4048 136
Size: 107 122 71 631 605 337 2039 1525
Fragments arranged by size:
2039 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2248 5432 2248
Size: 3184 2253
BlpI GC'TnA_GC
Cuts at: 2854 2854
Size: 5437
Bme1580I G_kGCm'C
Cuts at: 1790 2014 2148 2194 2525 3498 4654 1790
Size: 224 134 46 331 973 1156 2573
Fragments arranged by size:
2573 1156 973 331 224 134 46
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5437
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2024 2219 3168
Size: 416 53 83 399 24 607 195 949
Cuts at: 3168 4126 442
Size: 958 1753
Fragments arranged by size:
1753 958 949 607 416 399 195 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2069 1056
Size: 1013 4424
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2027 2042 2547 4097 1239
Size: 788 15 505 1550 2579
Fragments arranged by size:
2579 1550 788 505 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3562 4427 4725 3562
Size: 865 298 4274
Fragments arranged by size:
4274 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4100 882
Size: 617 2601 2219
Fragments arranged by size:
2601 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3228 493
Size: 711 2024 2702
Fragments arranged by size:
2702 2024 711
BsaBI GATnn'nnATC
Cuts at: 5431 5431
Size: 5437
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2367 2438 2508 2547
Size: 53 83 186 936 834 71 70 39
Cuts at: 2547 3244 3626 4261 275
Size: 697 382 635 1451
Fragments arranged by size:
1451 936 834 697 635 382 186 83
71 70 53 39
BsaWI w'CCGG_w
Cuts at: 2643 3870 4268 4542 4689 5100 2643
Size: 1227 398 274 147 411 2980
Fragments arranged by size:
2980 1227 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2151 2181 2545 2575 1178
Size: 30 165 30 748 30 364 30 4040
Fragments arranged by size:
4040 748 364 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2246 2249 1186
Size: 1060 3 4374
Fragments arranged by size:
4374 1060 3
BseYI C'CCAG_C
Cuts at: 2199 2266 2313 4640 5136 2199
Size: 67 47 2327 496 2500
Fragments arranged by size:
2500 2327 496 67 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2657 1163
Size: 573 921 3943
Fragments arranged by size:
3943 921 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2181 2887 3648 4676 1540
Size: 275 186 180 706 761 1028 2301
Fragments arranged by size:
2301 1028 761 706 275 186 180
BsiHKAI G_wGCw'C
Cuts at: 3498 3583 4654 3498
Size: 85 1071 4281
Fragments arranged by size:
4281 1071 85
BsmI GAATG_Cn'
Cuts at: 2948 3041 2948
Size: 93 5344
BsmBI CGTCTCn'nnnn_
Cuts at: 2168 2312 2546 2168
Size: 144 234 5059
Fragments arranged by size:
5059 234 144
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2014 2148 2194 2265 2525 2756 3498
Size: 399 224 134 46 71 260 231 742
Cuts at: 3498 3583 4654 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 260 231 224 134
85 71 46
BspEI T'CCGG_A
Cuts at: 2643 5100 2643
Size: 2457 2980
BspHI T'CATG_A
Cuts at: 3326 5056 3326
Size: 1730 3707
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2792 844
Size: 1948 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2349 2469 3324 1394
Size: 429 526 120 855 3507
Fragments arranged by size:
3507 855 526 429 120
BsrDI GCAATG_nn'
Cuts at: 65 1248 3932 4106 65
Size: 1183 2684 174 1396
Fragments arranged by size:
2684 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4081 4268 1700
Size: 110 2271 187 2869
Fragments arranged by size:
2869 2271 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5437
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5437
BssSI C'ACGA_G
Cuts at: 2611 3497 4509 2611
Size: 886 1012 3539
Fragments arranged by size:
3539 1012 886
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5437
BstBI TT'CG_AA
Cuts at: 4241 4241
Size: 5437
BstEII G'GTnAC_C
Cuts at: 1184 2092 1184
Size: 908 4529
BstXI CCAn_nnnn'nTGG
Cuts at: 2089 2089
Size: 5437
BstYI r'GATC_y
Cuts at: 1972 2248 2767 3134 3519 3536 4988 5074 5086
Size: 276 519 367 385 17 1452 86 12
Cuts at: 5086 5432 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 519 385 367 346 276 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3946
Fragments arranged by size:
3946 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4698
Fragments arranged by size:
4698 683 56
BtsI GCAGTG_nn'
Cuts at: 2953 3747 3767 2953
Size: 794 20 4623
Fragments arranged by size:
4623 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5437
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5402
DraI TTT'AAA
Cuts at: 2741 2741
Size: 5437
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4444 809
Size: 507 3128 1802
Fragments arranged by size:
3128 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2178 2577 2884
Size: 54 1425 50 275 18 348 399 307
Cuts at: 2884 3773 5402 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 399 348 307 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2178 2884 1537
Size: 275 366 706 4090
Fragments arranged by size:
4090 706 366 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3367 1353
Size: 2014 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4024 4398 4544 387
Size: 1064 138 2435 374 146 1280
Fragments arranged by size:
2435 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2027 2042 2315 2547 3500 4097 4884
Size: 393 395 15 273 232 953 597 787
Cuts at: 4884 5152 1239
Size: 268 1524
Fragments arranged by size:
1524 953 787 597 395 393 273 268
232 15
EcoO109I rG'GnC_Cy
Cuts at: 2455 2455
Size: 5437
EcoRI G'AATT_C
Cuts at: 2746 3124 2746
Size: 378 5059
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2731 2731
Size: 5437
FspI TGC'GCA
Cuts at: 2483 3943 2483
Size: 1460 3977
HaeII r_GCGC'y
Cuts at: 1678 2289 2384 2441 2511 2550 4584 1678
Size: 611 95 57 70 39 2034 2531
Fragments arranged by size:
2531 2034 611 95 70 57 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2545 2577 4078 4110 4152
Size: 32 135 32 973 32 1501 32 42
Cuts at: 4152 4184 1373
Size: 32 2626
Fragments arranged by size:
2626 1501 973 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2668 2781 3027 669
Size: 988 281 730 113 246 3079
Fragments arranged by size:
3079 988 730 281 246 113
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5437
HpaI GTT'AAC
Cuts at: 3027 3027
Size: 5437
KasI G'GCGC_C
Cuts at: 2437 2507 2546 2437
Size: 70 39 5328
Fragments arranged by size:
5328 70 39
KpnI G_GTAC'C
Cuts at: 2762 2762
Size: 5437
MfeI C'AATT_G
Cuts at: 3036 3036
Size: 5437
MluI A'CGCG_T
Cuts at: 5313 5313
Size: 5437
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4551 4735 1305
Size: 3246 184 2007
Fragments arranged by size:
3246 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3615
Fragments arranged by size:
3615 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2615 3395 3754 3913 518
Size: 648 1449 780 359 159 2042
Fragments arranged by size:
2042 1449 780 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2270 2293 2853 3530 4678 4923 1606
Size: 664 23 560 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 664 560 245 23
NarI GG'CG_CC
Cuts at: 2438 2508 2547 2438
Size: 70 39 5328
Fragments arranged by size:
5328 70 39
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3946
Fragments arranged by size:
3946 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5437
NheI G'CTAG_C
Cuts at: 1052 2065 1052
Size: 1013 4424
NotI GC'GGCC_GC
Cuts at: 2884 2884
Size: 5437
NruI TCG'CGA
Cuts at: 2699 2699
Size: 5437
NspI r_CATG'y
Cuts at: 1132 1229 2795 4340 1132
Size: 97 1566 1545 2229
Fragments arranged by size:
2229 1566 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2461 1348
Size: 1113 4324
PciI A'CATG_T
Cuts at: 4336 4336
Size: 5437
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5437
PmeI GTTT'AAAC
Cuts at: 2741 2741
Size: 5437
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3506 3538 5019 5051 3506
Size: 32 1481 32 3892
Fragments arranged by size:
3892 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2455 2455
Size: 5437
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5437
PsiI TTA'TAA
Cuts at: 3007 3007
Size: 5437
PspXI vC'TCGA_Gb
Cuts at: 2540 2540
Size: 5437
PstI C_TGCA'G
Cuts at: 830 2789 830
Size: 1959 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5437
PvuII CAG'CTG
Cuts at: 2270 2270
Size: 5437
SalI G'TCGA_C
Cuts at: 1936 2779 1936
Size: 843 4594
SanDI GG'GwC_CC
Cuts at: 2455 2455
Size: 5437
SbfI CC_TGCA'GG
Cuts at: 2789 2789
Size: 5437
ScaI AGT'ACT
Cuts at: 3685 5397 3685
Size: 1712 3725
SfcI C'TryA_G
Cuts at: 826 1045 2785 3920 4601 4792 826
Size: 219 1740 1135 681 191 1471
Fragments arranged by size:
1740 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2439 2509 2548 2439
Size: 70 39 5328
Fragments arranged by size:
5328 70 39
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5437
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5437
SmaI CCC'GGG
Cuts at: 1872 2188 2260 2764 1872
Size: 316 72 504 4545
Fragments arranged by size:
4545 504 316 72
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2540 3541 4442 4704 820
Size: 197 926 597 1001 901 262 1553
Fragments arranged by size:
1553 1001 926 901 597 262 197
SnaBI TAC'GTA
Cuts at: 493 3228 493
Size: 2735 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5437
SphI G_CATG'C
Cuts at: 1132 2795 1132
Size: 1663 3774
SspI AAT'ATT
Cuts at: 5 52 3361 5
Size: 47 3309 2081
Fragments arranged by size:
3309 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5437
StyI C'CwwG_G
Cuts at: 513 1065 2004 2876 513
Size: 552 939 872 3074
Fragments arranged by size:
3074 939 872 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2461 3464 3481 3634 3819 4264 325
Size: 1255 881 1003 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1003 881 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3683 5395 96
Size: 274 80 33 51 1443 1706 1712 138
Fragments arranged by size:
1712 1706 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5397 5429 5397
Size: 32 5405
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5437
XbaI T'CTAG_A
Cuts at: 2773 2773
Size: 5437
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5437
XhoI C'TCGA_G
Cuts at: 1943 2540 1943
Size: 597 4840
XmaI C'CCGG_G
Cuts at: 1870 2186 2258 2762 1870
Size: 316 72 504 4545
Fragments arranged by size:
4545 504 316 72
XmnI GAAnn'nnTTC
Cuts at: 2711 3566 2711
Size: 855 4582
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3245 276
Size: 53 83 186 936 1711 2468
Fragments arranged by size:
2468 1711 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NruI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspXI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI NaeI NgoMIV NsiI PacI
PfoI PmlI PspOMI PsrI RsrII SacI SacII SapI
SexAI SfiI SrfI SwaI