Restriction map for
pFN21AE1737
(Circular) (Six-base) MAPSORT of: pFN21AE1737.seq Check: 8121 from: 1 to: 5434
pFN21AE1737 5434 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3244 278
Size: 53 83 186 936 1708 2468
Fragments arranged by size:
2468 1708 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2777 1937
Size: 840 4594
Acc65I G'GTAC_C
Cuts at: 2286 2755 2286
Size: 469 4965
AclI AA'CG_TT
Cuts at: 1563 3561 3934 1563
Size: 1998 373 3063
Fragments arranged by size:
3063 1998 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2281 2534 3497 4881 5149 1632
Size: 649 253 963 1384 268 1917
Fragments arranged by size:
1917 1384 963 649 268 253
AfeI AGC'GCT
Cuts at: 2043 2043
Size: 5434
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5237
AflIII A'CryG_T
Cuts at: 1203 4333 5310 1203
Size: 3130 977 1327
Fragments arranged by size:
3130 1327 977
AgeI A'CCGG_T
Cuts at: 4265 4265
Size: 5434
AhdI GACnn_n'nnGTC
Cuts at: 4163 4163
Size: 5434
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5434
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5434
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2579 2611 2579
Size: 32 5402
AlwNI CAG_nnn'CTG
Cuts at: 3177 4749 3177
Size: 1572 3862
ApaLI G'TGCA_C
Cuts at: 3491 4647 3491
Size: 1156 4278
ApoI r'AATT_y
Cuts at: 1493 2743 2969 3121 5230 1493
Size: 1250 226 152 2109 1697
Fragments arranged by size:
2109 1697 1250 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3988 160
Size: 872 2956 1606
Fragments arranged by size:
2956 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5434
AvaI C'yCGr_G
Cuts at: 1870 1943 2011 2759 1870
Size: 73 68 748 4545
Fragments arranged by size:
4545 748 73 68
BamHI G'GATC_C
Cuts at: 2269 2764 3131 2269
Size: 495 367 4572
Fragments arranged by size:
4572 495 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2221 2286 2453 2755 4210
Size: 325 217 627 434 65 167 302 1455
Cuts at: 4210 618
Size: 1842
Fragments arranged by size:
1842 1455 627 434 325 302 217 167
65
BanII G_rGCy'C
Cuts at: 1391 2012 2753 1391
Size: 621 741 4072
Fragments arranged by size:
4072 741 621
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2522 928
Size: 971 623 3840
Fragments arranged by size:
3840 971 623
BbvCI CC'TCA_GC
Cuts at: 2495 2495
Size: 5434
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3625 3659 1903
Size: 34 1688 34 3678
Fragments arranged by size:
3678 1688 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3326 4536 3326
Size: 1210 4224
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5434
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4045 136
Size: 107 122 71 631 605 2373 1525
Fragments arranged by size:
2373 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5429 5429
Size: 5434
BlpI GC'TnA_GC
Cuts at: 2851 2851
Size: 5434
Bme1580I G_kGCm'C
Cuts at: 1790 2458 3495 4651 1790
Size: 668 1037 1156 2573
Fragments arranged by size:
2573 1156 1037 668
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5434
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2374 3165 4123
Size: 416 53 83 399 24 957 791 958
Cuts at: 4123 442
Size: 1753
Fragments arranged by size:
1753 958 957 791 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5434
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2510 2510
Size: 5434
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2285 2624 4094 1239
Size: 1046 339 1470 2579
Fragments arranged by size:
2579 1470 1046 339
Bpu10I CC'TnA_GC
Cuts at: 2495 2495
Size: 5434
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3559 4424 4722 3559
Size: 865 298 4271
Fragments arranged by size:
4271 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2540 4097 882
Size: 617 1041 1557 2219
Fragments arranged by size:
2219 1557 1041 617
BsaAI yAC'GTr
Cuts at: 493 1204 2704 3225 493
Size: 711 1500 521 2702
Fragments arranged by size:
2702 1500 711 521
BsaBI GATnn'nnATC
Cuts at: 5428 5428
Size: 5434
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3241 3623 4258 275
Size: 53 83 186 936 1708 382 635 1451
Fragments arranged by size:
1708 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2695 3867 4265 4539 4686 5097 2695
Size: 1172 398 274 147 411 3032
Fragments arranged by size:
3032 1172 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5209
Fragments arranged by size:
5209 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2696 1186
Size: 1510 3924
BseYI C'CCAG_C
Cuts at: 2258 2457 4637 5133 2258
Size: 199 2180 496 2559
Fragments arranged by size:
2559 2180 496 199
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4861
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2884 3645 4673 1540
Size: 275 186 883 761 1028 2301
Fragments arranged by size:
2301 1028 883 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 2424 3495 3580 4651 2012
Size: 412 1071 85 1071 2795
Fragments arranged by size:
2795 1071 1071 412 85
BsmI GAATG_Cn'
Cuts at: 2945 3038 2945
Size: 93 5341
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2424 2458 2753 3495 3580 4651
Size: 399 222 412 34 295 742 85 1071
Cuts at: 4651 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 412 399 295 222 85
34
BspEI T'CCGG_A
Cuts at: 5097 5097
Size: 5434
BspHI T'CATG_A
Cuts at: 3323 5053 3323
Size: 1730 3704
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2789 844
Size: 1945 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3321 1394
Size: 429 1498 3507
Fragments arranged by size:
3507 1498 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2343 3929 4103 65
Size: 1183 1095 1586 174 1396
Fragments arranged by size:
1586 1396 1183 1095 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4078 4265 1700
Size: 110 2268 187 2869
Fragments arranged by size:
2869 2268 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5434
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5434
BssSI C'ACGA_G
Cuts at: 3494 4506 3494
Size: 1012 4422
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5434
BstBI TT'CG_AA
Cuts at: 4238 4238
Size: 5434
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5434
BstXI CCAn_nnnn'nTGG
Cuts at: 2350 2350
Size: 5434
BstYI r'GATC_y
Cuts at: 1972 2269 2764 3131 3516 3533 4985 5071 5083
Size: 297 495 367 385 17 1452 86 12
Cuts at: 5083 5429 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 495 385 367 346 297 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2349 513
Size: 552 1284 3598
Fragments arranged by size:
3598 1284 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4695
Fragments arranged by size:
4695 683 56
BtsI GCAGTG_nn'
Cuts at: 2950 3744 3764 2950
Size: 794 20 4620
Fragments arranged by size:
4620 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5434
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5399
DraI TTT'AAA
Cuts at: 2738 2738
Size: 5434
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4441 809
Size: 507 3125 1802
Fragments arranged by size:
3125 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2198 2881 3770
Size: 54 1425 50 275 18 368 683 889
Cuts at: 3770 5399 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 683 368 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2881 1537
Size: 275 1069 4090
Fragments arranged by size:
4090 1069 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3364 1353
Size: 2011 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2228 4021 4395 4541 387
Size: 1064 138 639 1793 374 146 1280
Fragments arranged by size:
1793 1280 1064 639 374 146 138
EcoICRI GAG'CTC
Cuts at: 2010 2010
Size: 5434
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2281 2285 2534 2624 3497 4094 4881
Size: 393 649 4 249 90 873 597 787
Cuts at: 4881 5149 1239
Size: 268 1524
Fragments arranged by size:
1524 873 787 649 597 393 268 249
90 4
EcoO109I rG'GnC_Cy
Cuts at: 2166 2204 2491 2166
Size: 38 287 5109
Fragments arranged by size:
5109 287 38
EcoRI G'AATT_C
Cuts at: 2743 3121 2743
Size: 378 5056
FspI TGC'GCA
Cuts at: 3940 3940
Size: 5434
HaeII r_GCGC'y
Cuts at: 1678 2045 4581 1678
Size: 367 2536 2531
Fragments arranged by size:
2536 2531 367
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2261 2293 2555 2587 4075
Size: 32 135 32 689 32 262 32 1488
Cuts at: 4075 4107 4149 4181 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1488 689 262 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2778 3024 669
Size: 988 281 840 246 3079
Fragments arranged by size:
3079 988 840 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5434
HpaI GTT'AAC
Cuts at: 3024 3024
Size: 5434
KpnI G_GTAC'C
Cuts at: 2290 2759 2290
Size: 469 4965
MfeI C'AATT_G
Cuts at: 3033 3033
Size: 5434
MluI A'CGCG_T
Cuts at: 5310 5310
Size: 5434
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2301 4548 4732 1305
Size: 996 2247 184 2007
Fragments arranged by size:
2247 2007 996 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2200 10
Size: 54 1425 343 368 3244
Fragments arranged by size:
3244 1425 368 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2348 3392 3751 3910 518
Size: 648 1182 1044 359 159 2042
Fragments arranged by size:
2042 1182 1044 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2850 3527 4675 4920 1606
Size: 1244 677 1148 245 2120
Fragments arranged by size:
2120 1244 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2349 513
Size: 552 1284 3598
Fragments arranged by size:
3598 1284 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5434
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5434
NotI GC'GGCC_GC
Cuts at: 2881 2881
Size: 5434
NspI r_CATG'y
Cuts at: 1132 1229 2792 4337 1132
Size: 97 1563 1545 2229
Fragments arranged by size:
2229 1563 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5434
PciI A'CATG_T
Cuts at: 4333 4333
Size: 5434
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5434
PfoI T'CCnGG_A
Cuts at: 2168 2618 2168
Size: 450 4984
PmeI GTTT'AAAC
Cuts at: 2738 2738
Size: 5434
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3503 3535 5016 5048 3503
Size: 32 1481 32 3889
Fragments arranged by size:
3889 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2166 2204 2491 2166
Size: 38 287 5109
Fragments arranged by size:
5109 287 38
PshAI GACnn'nnGTC
Cuts at: 1613 2317 1613
Size: 704 4730
PsiI TTA'TAA
Cuts at: 3004 3004
Size: 5434
PstI C_TGCA'G
Cuts at: 830 2486 2786 830
Size: 1656 300 3478
Fragments arranged by size:
3478 1656 300
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5434
SacI G_AGCT'C
Cuts at: 2012 2012
Size: 5434
SalI G'TCGA_C
Cuts at: 1936 2776 1936
Size: 840 4594
SanDI GG'GwC_CC
Cuts at: 2166 2166
Size: 5434
SbfI CC_TGCA'GG
Cuts at: 2786 2786
Size: 5434
ScaI AGT'ACT
Cuts at: 3682 5394 3682
Size: 1712 3722
SfcI C'TryA_G
Cuts at: 826 1045 2482 2782 3917 4598 4789 826
Size: 219 1437 300 1135 681 191 1471
Fragments arranged by size:
1471 1437 1135 681 300 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5434
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5434
SmaI CCC'GGG
Cuts at: 1872 2761 1872
Size: 889 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3538 4439 4701 820
Size: 197 926 1595 901 262 1553
Fragments arranged by size:
1595 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3225 493
Size: 2732 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5434
SphI G_CATG'C
Cuts at: 1132 2792 1132
Size: 1660 3774
SspI AAT'ATT
Cuts at: 5 52 3358 5
Size: 47 3306 2081
Fragments arranged by size:
3306 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5434
StyI C'CwwG_G
Cuts at: 513 1065 2181 2349 2873 513
Size: 552 1116 168 524 3074
Fragments arranged by size:
3074 1116 552 524 168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2239 3461 3478 3631 3816 4261 325
Size: 1255 659 1222 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1222 659 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3680 5392 96
Size: 274 80 33 51 1443 1703 1712 138
Fragments arranged by size:
1712 1703 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5394 5426 5394
Size: 32 5402
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5434
XbaI T'CTAG_A
Cuts at: 2770 2770
Size: 5434
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2626 2626
Size: 5434
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5434
XmaI C'CCGG_G
Cuts at: 1870 2759 1870
Size: 889 4545
XmnI GAAnn'nnTTC
Cuts at: 3563 3563
Size: 5434
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3242 276
Size: 53 83 186 936 1708 2468
Fragments arranged by size:
2468 1708 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI
SexAI SfiI SfoI SrfI SwaI