Restriction map for
pFN21AE1736
(Circular) (Six-base) MAPSORT of: pFN21AE1736.seq Check: 4225 from: 1 to: 5431
pFN21AE1736 5431 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2324 2324
Size: 5431
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2587 3241 278
Size: 53 83 186 936 1051 654 2468
Fragments arranged by size:
2468 1051 936 654 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2774 1937
Size: 837 4594
Acc65I G'GTAC_C
Cuts at: 2485 2752 2485
Size: 267 5164
AclI AA'CG_TT
Cuts at: 1563 3558 3931 1563
Size: 1995 373 3063
Fragments arranged by size:
3063 1995 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2169 2235 3494 4878 5146 1632
Size: 537 66 1259 1384 268 1917
Fragments arranged by size:
1917 1384 1259 537 268 66
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5234
AflIII A'CryG_T
Cuts at: 1203 2220 4330 5307 1203
Size: 1017 2110 977 1327
Fragments arranged by size:
2110 1327 1017 977
AgeI A'CCGG_T
Cuts at: 4262 4262
Size: 5431
AhdI GACnn_n'nnGTC
Cuts at: 4160 4160
Size: 5431
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5431
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5431
AlwNI CAG_nnn'CTG
Cuts at: 3174 4746 3174
Size: 1572 3859
ApaLI G'TGCA_C
Cuts at: 3488 4644 3488
Size: 1156 4275
ApoI r'AATT_y
Cuts at: 1493 2205 2740 2966 3118 5227 1493
Size: 712 535 226 152 2109 1697
Fragments arranged by size:
2109 1697 712 535 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3985 160
Size: 872 2953 1606
Fragments arranged by size:
2953 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5431
AvaI C'yCGr_G
Cuts at: 1870 1943 2022 2756 1870
Size: 73 79 734 4545
Fragments arranged by size:
4545 734 79 73
BamHI G'GATC_C
Cuts at: 2761 3128 2761
Size: 367 5064
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2485 2752 4207 618
Size: 325 217 627 698 267 1455 1842
Fragments arranged by size:
1842 1455 698 627 325 267 217
BanII G_rGCy'C
Cuts at: 1391 2750 1391
Size: 1359 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2223 928
Size: 971 324 4136
Fragments arranged by size:
4136 971 324
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3622 3656 1903
Size: 34 1685 34 3678
Fragments arranged by size:
3678 1685 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3323 4533 3323
Size: 1210 4221
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5431
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4042 136
Size: 107 122 71 631 605 2370 1525
Fragments arranged by size:
2370 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5426 5426
Size: 5431
BlpI GC'TnA_GC
Cuts at: 2848 2848
Size: 5431
Bme1580I G_kGCm'C
Cuts at: 1790 3492 4648 1790
Size: 1702 1156 2573
Fragments arranged by size:
2573 1702 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5431
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2139 2702 3162
Size: 416 53 83 399 24 722 563 460
Cuts at: 3162 4120 442
Size: 958 1753
Fragments arranged by size:
1753 958 722 563 460 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5431
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2157 4091 1239
Size: 918 1934 2579
Fragments arranged by size:
2579 1934 918
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3556 4421 4719 3556
Size: 865 298 4268
Fragments arranged by size:
4268 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4094 882
Size: 617 2595 2219
Fragments arranged by size:
2595 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3222 493
Size: 711 2018 2702
Fragments arranged by size:
2702 2018 711
BsaBI GATnn'nnATC
Cuts at: 5425 5425
Size: 5431
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2584 3238 3620 4255
Size: 53 83 186 936 1051 654 382 635
Cuts at: 4255 275
Size: 1451
Fragments arranged by size:
1451 1051 936 654 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2450 3864 4262 4536 4683 5094 2450
Size: 1414 398 274 147 411 2787
Fragments arranged by size:
2787 1414 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2546 2576 2583 2613 1178
Size: 30 165 30 1143 30 7 30 3996
Fragments arranged by size:
3996 1143 165 30 30 30 30 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2154 2289 2343 2525 1186
Size: 968 135 54 182 4092
Fragments arranged by size:
4092 968 182 135 54
BseYI C'CCAG_C
Cuts at: 2121 4634 5130 2121
Size: 2513 496 2422
Fragments arranged by size:
2513 2422 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2358 2589 1163
Size: 573 622 231 4005
Fragments arranged by size:
4005 622 573 231
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2881 3642 4670 1540
Size: 275 186 880 761 1028 2301
Fragments arranged by size:
2301 1028 880 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3492 3577 4648 3492
Size: 85 1071 4275
Fragments arranged by size:
4275 1071 85
BsmI GAATG_Cn'
Cuts at: 2317 2942 3035 2317
Size: 625 93 4713
Fragments arranged by size:
4713 625 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2750 3492 3577 4648 1391
Size: 399 960 742 85 1071 2174
Fragments arranged by size:
2174 1071 960 742 399 85
BspEI T'CCGG_A
Cuts at: 2450 5094 2450
Size: 2644 2787
BspHI T'CATG_A
Cuts at: 2307 3320 5050 2307
Size: 1013 1730 2688
Fragments arranged by size:
2688 1730 1013
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2324 2786 844
Size: 1480 462 3489
Fragments arranged by size:
3489 1480 462
BsrBI CCG'CTC
Cuts at: 1394 1823 3318 1394
Size: 429 1495 3507
Fragments arranged by size:
3507 1495 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2175 2550 3926 4100 65
Size: 1183 927 375 1376 174 1396
Fragments arranged by size:
1396 1376 1183 927 375 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4075 4262 1700
Size: 110 2265 187 2869
Fragments arranged by size:
2869 2265 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5431
BssHII G'CGCG_C
Cuts at: 1925 2055 1925
Size: 130 5301
BssSI C'ACGA_G
Cuts at: 3491 4503 3491
Size: 1012 4419
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5431
BstBI TT'CG_AA
Cuts at: 4235 4235
Size: 5431
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5431
BstYI r'GATC_y
Cuts at: 1972 2761 3128 3513 3530 4982 5068 5080 5426
Size: 789 367 385 17 1452 86 12 346
Cuts at: 5426 1972
Size: 1977
Fragments arranged by size:
1977 1452 789 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2508 2508
Size: 5431
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4879
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2178 487
Size: 683 56 952 3740
Fragments arranged by size:
3740 952 683 56
BtsI GCAGTG_nn'
Cuts at: 2346 2947 3741 3761 2346
Size: 601 794 20 4016
Fragments arranged by size:
4016 794 601 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5431
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5396
DraI TTT'AAA
Cuts at: 2404 2735 2404
Size: 331 5100
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4438 809
Size: 507 3122 1802
Fragments arranged by size:
3122 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2373 2878 3767
Size: 54 1425 50 275 18 543 505 889
Cuts at: 3767 5396 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 543 505 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2878 1537
Size: 275 1066 4090
Fragments arranged by size:
4090 1066 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3361 1353
Size: 2008 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4018 4392 4538 387
Size: 1064 138 2429 374 146 1280
Fragments arranged by size:
2429 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2157 2169 2235 3494 4091 4878 5146
Size: 393 525 12 66 1259 597 787 268
Cuts at: 5146 1239
Size: 1524
Fragments arranged by size:
1524 1259 787 597 525 393 268 66
12
EcoRI G'AATT_C
Cuts at: 2205 2740 3118 2205
Size: 535 378 4518
Fragments arranged by size:
4518 535 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2277 2705 2277
Size: 428 5003
FspI TGC'GCA
Cuts at: 3937 3937
Size: 5431
HaeII r_GCGC'y
Cuts at: 1678 4578 1678
Size: 2900 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2070 2102 4072 4104 4146
Size: 32 135 32 498 32 1970 32 42
Cuts at: 4146 4178 1373
Size: 32 2626
Fragments arranged by size:
2626 1970 498 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2775 3021 669
Size: 988 281 837 246 3079
Fragments arranged by size:
3079 988 837 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5431
HpaI GTT'AAC
Cuts at: 3021 3021
Size: 5431
KpnI G_GTAC'C
Cuts at: 2489 2756 2489
Size: 267 5164
MfeI C'AATT_G
Cuts at: 3030 3030
Size: 5431
MluI A'CGCG_T
Cuts at: 5307 5307
Size: 5431
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2692 4545 4729 1305
Size: 1387 1853 184 2007
Fragments arranged by size:
2007 1853 1387 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2375 10
Size: 54 1425 343 543 3066
Fragments arranged by size:
3066 1425 543 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2163 3389 3748 3907 518
Size: 648 997 1226 359 159 2042
Fragments arranged by size:
2042 1226 997 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2847 3524 4672 4917 1606
Size: 1241 677 1148 245 2120
Fragments arranged by size:
2120 1241 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4879
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5431
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5431
NotI GC'GGCC_GC
Cuts at: 2878 2878
Size: 5431
NspI r_CATG'y
Cuts at: 1132 1229 2224 2789 4334 1132
Size: 97 995 565 1545 2229
Fragments arranged by size:
2229 1545 995 565 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5431
PciI A'CATG_T
Cuts at: 2220 4330 2220
Size: 2110 3321
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5431
PfoI T'CCnGG_A
Cuts at: 2042 2389 2042
Size: 347 5084
PmeI GTTT'AAAC
Cuts at: 2735 2735
Size: 5431
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3500 3532 5013 5045 3500
Size: 32 1481 32 3886
Fragments arranged by size:
3886 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5431
PsiI TTA'TAA
Cuts at: 3001 3001
Size: 5431
PstI C_TGCA'G
Cuts at: 830 2783 830
Size: 1953 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5431
SalI G'TCGA_C
Cuts at: 1936 2773 1936
Size: 837 4594
SbfI CC_TGCA'GG
Cuts at: 2783 2783
Size: 5431
ScaI AGT'ACT
Cuts at: 2574 3679 5391 2574
Size: 1105 1712 2614
Fragments arranged by size:
2614 1712 1105
SfcI C'TryA_G
Cuts at: 826 1045 2779 3914 4595 4786 826
Size: 219 1734 1135 681 191 1471
Fragments arranged by size:
1734 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5431
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5431
SmaI CCC'GGG
Cuts at: 1872 2758 1872
Size: 886 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3535 4436 4698 820
Size: 197 926 1592 901 262 1553
Fragments arranged by size:
1592 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3222 493
Size: 2729 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5431
SphI G_CATG'C
Cuts at: 1132 2789 1132
Size: 1657 3774
SspI AAT'ATT
Cuts at: 5 52 3355 5
Size: 47 3303 2081
Fragments arranged by size:
3303 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5431
StyI C'CwwG_G
Cuts at: 513 1065 2870 513
Size: 552 1805 3074
Fragments arranged by size:
3074 1805 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3458 3475 3628 3813 4258 325
Size: 1255 1878 17 153 185 445 1498
Fragments arranged by size:
1878 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2294 2444 2572
Size: 274 80 33 51 1443 317 150 128
Cuts at: 2572 3677 5389 96
Size: 1105 1712 138
Fragments arranged by size:
1712 1443 1105 317 274 150 138 128
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2583 2615 5391 5423 2583
Size: 32 2776 32 2591
Fragments arranged by size:
2776 2591 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5431
XbaI T'CTAG_A
Cuts at: 2767 2767
Size: 5431
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5431
XmaI C'CCGG_G
Cuts at: 1870 2756 1870
Size: 886 4545
XmnI GAAnn'nnTTC
Cuts at: 2205 3560 2205
Size: 1355 4076
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2585 3239 276
Size: 53 83 186 936 1051 654 2468
Fragments arranged by size:
2468 1051 936 654 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI