Restriction map for
pFN21AE1735
(Circular) (Six-base) MAPSORT of: pFN21AE1735.seq Check: 6860 from: 1 to: 5431
pFN21AE1735 5431 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3241 278
Size: 53 83 186 936 1705 2468
Fragments arranged by size:
2468 1705 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2774 1937
Size: 837 4594
Acc65I G'GTAC_C
Cuts at: 2752 2752
Size: 5431
AclI AA'CG_TT
Cuts at: 1563 3558 3931 1563
Size: 1995 373 3063
Fragments arranged by size:
3063 1995 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3494 4878 5146 1632
Size: 1862 1384 268 1917
Fragments arranged by size:
1917 1862 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5234
AflIII A'CryG_T
Cuts at: 1203 2127 4330 5307 1203
Size: 924 2203 977 1327
Fragments arranged by size:
2203 1327 977 924
AgeI A'CCGG_T
Cuts at: 4262 4262
Size: 5431
AhdI GACnn_n'nnGTC
Cuts at: 4160 4160
Size: 5431
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5431
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5431
AlwNI CAG_nnn'CTG
Cuts at: 2362 2575 3174 4746 2362
Size: 213 599 1572 3047
Fragments arranged by size:
3047 1572 599 213
ApaLI G'TGCA_C
Cuts at: 3488 4644 3488
Size: 1156 4275
ApoI r'AATT_y
Cuts at: 1493 2204 2740 2966 3118 5227 1493
Size: 711 536 226 152 2109 1697
Fragments arranged by size:
2109 1697 711 536 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3985 160
Size: 872 2953 1606
Fragments arranged by size:
2953 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5431
AvaI C'yCGr_G
Cuts at: 1870 1943 2088 2226 2756 1870
Size: 73 145 138 530 4545
Fragments arranged by size:
4545 530 145 138 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2705 2738 2705
Size: 33 5398
BamHI G'GATC_C
Cuts at: 2761 3128 2761
Size: 367 5064
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2051 2752 4207 618
Size: 325 217 627 264 701 1455 1842
Fragments arranged by size:
1842 1455 701 627 325 264 217
BanII G_rGCy'C
Cuts at: 1391 2750 1391
Size: 1359 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2112 928
Size: 971 213 4247
Fragments arranged by size:
4247 971 213
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3622 3656 1903
Size: 34 1685 34 3678
Fragments arranged by size:
3678 1685 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2519 3323 4533 2519
Size: 804 1210 3417
Fragments arranged by size:
3417 1210 804
BclI T'GATC_A
Cuts at: 1548 2545 1548
Size: 997 4434
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2060 4042 136
Size: 107 122 71 631 605 388 1982 1525
Fragments arranged by size:
1982 1525 631 605 388 122 107 71
BglII A'GATC_T
Cuts at: 5426 5426
Size: 5431
BlpI GC'TnA_GC
Cuts at: 2370 2848 2370
Size: 478 4953
Bme1580I G_kGCm'C
Cuts at: 1790 2056 3492 4648 1790
Size: 266 1436 1156 2573
Fragments arranged by size:
2573 1436 1156 266
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5431
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3162 4120 442
Size: 416 53 83 399 24 1745 958 1753
Fragments arranged by size:
1753 1745 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5431
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2339 4091 1239
Size: 1100 1752 2579
Fragments arranged by size:
2579 1752 1100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2273 3556 4421 4719 2273
Size: 1283 865 298 2985
Fragments arranged by size:
2985 1283 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2160 4094 882
Size: 617 661 1934 2219
Fragments arranged by size:
2219 1934 661 617
BsaAI yAC'GTr
Cuts at: 493 1204 3222 493
Size: 711 2018 2702
Fragments arranged by size:
2702 2018 711
BsaBI GATnn'nnATC
Cuts at: 2456 5425 2456
Size: 2969 2462
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3238 3620 4255 275
Size: 53 83 186 936 1705 382 635 1451
Fragments arranged by size:
1705 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2709 3864 4262 4536 4683 5094 2709
Size: 1155 398 274 147 411 3046
Fragments arranged by size:
3046 1155 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5206
Fragments arranged by size:
5206 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2045 2418 1186
Size: 859 373 4199
Fragments arranged by size:
4199 859 373
BseYI C'CCAG_C
Cuts at: 4634 5130 4634
Size: 496 4935
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4858
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2881 3642 4670 1540
Size: 275 186 880 761 1028 2301
Fragments arranged by size:
2301 1028 880 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3492 3577 4648 3492
Size: 85 1071 4275
Fragments arranged by size:
4275 1071 85
BsmI GAATG_Cn'
Cuts at: 2942 3035 2942
Size: 93 5338
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2056 2750 3492 3577 4648 1391
Size: 399 266 694 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 694 399 266 85
BspEI T'CCGG_A
Cuts at: 2709 5094 2709
Size: 2385 3046
BspHI T'CATG_A
Cuts at: 3320 5050 3320
Size: 1730 3701
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2719 2786 844
Size: 1875 67 3489
Fragments arranged by size:
3489 1875 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3318 1394
Size: 429 1495 3507
Fragments arranged by size:
3507 1495 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3926 4100 65
Size: 1183 2678 174 1396
Fragments arranged by size:
2678 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4075 4262 1700
Size: 110 2265 187 2869
Fragments arranged by size:
2869 2265 187 110
BsrGI T'GTAC_A
Cuts at: 96 2382 96
Size: 2286 3145
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5431
BssSI C'ACGA_G
Cuts at: 3491 4503 3491
Size: 1012 4419
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5431
BstBI TT'CG_AA
Cuts at: 2148 4235 2148
Size: 2087 3344
BstEII G'GTnAC_C
Cuts at: 1184 2629 1184
Size: 1445 3986
BstYI r'GATC_y
Cuts at: 1972 2233 2761 3128 3513 3530 4982 5068 5080
Size: 261 528 367 385 17 1452 86 12
Cuts at: 5080 5426 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 528 385 367 346 261 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2015 2514 513
Size: 552 950 499 3430
Fragments arranged by size:
3430 950 552 499
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2515 487
Size: 683 56 1289 3403
Fragments arranged by size:
3403 1289 683 56
BtsI GCAGTG_nn'
Cuts at: 2172 2947 3741 3761 2172
Size: 775 794 20 3842
Fragments arranged by size:
3842 794 775 20
ClaI AT'CG_AT
Cuts at: 1554 2220 1554
Size: 666 4765
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5396
DraI TTT'AAA
Cuts at: 2735 2735
Size: 5431
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4438 809
Size: 507 3122 1802
Fragments arranged by size:
3122 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2878 3767 5396
Size: 54 1425 50 275 18 1048 889 1629
Cuts at: 5396 8
Size: 43
Fragments arranged by size:
1629 1425 1048 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2878 1537
Size: 275 1066 4090
Fragments arranged by size:
4090 1066 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2022 2187 2333 2588 3361 1353
Size: 669 165 146 255 773 3423
Fragments arranged by size:
3423 773 669 255 165 146
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2653 4018 4392 4538 387
Size: 1064 138 1064 1365 374 146 1280
Fragments arranged by size:
1365 1280 1064 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2339 3494 4091 4878 5146 1239
Size: 393 707 1155 597 787 268 1524
Fragments arranged by size:
1524 1155 787 707 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2335 2359 2416 2335
Size: 24 57 5350
Fragments arranged by size:
5350 57 24
EcoRI G'AATT_C
Cuts at: 2740 3118 2740
Size: 378 5053
FspI TGC'GCA
Cuts at: 3937 3937
Size: 5431
HaeII r_GCGC'y
Cuts at: 1678 4578 1678
Size: 2900 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4072 4104 4146 4178 1373
Size: 32 135 32 2500 32 42 32 2626
Fragments arranged by size:
2626 2500 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2775 3021 669
Size: 988 281 837 246 3079
Fragments arranged by size:
3079 988 837 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5431
HpaI GTT'AAC
Cuts at: 3021 3021
Size: 5431
KpnI G_GTAC'C
Cuts at: 2756 2756
Size: 5431
MfeI C'AATT_G
Cuts at: 3030 3030
Size: 5431
MluI A'CGCG_T
Cuts at: 5307 5307
Size: 5431
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4545 4729 1305
Size: 3240 184 2007
Fragments arranged by size:
3240 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3609
Fragments arranged by size:
3609 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3389 3748 3907 518
Size: 648 2223 359 159 2042
Fragments arranged by size:
2223 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2017 2140 2847 3524 4672 4917 1606
Size: 411 123 707 677 1148 245 2120
Fragments arranged by size:
2120 1148 707 677 411 245 123
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4879
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5431
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5431
NotI GC'GGCC_GC
Cuts at: 2878 2878
Size: 5431
NspI r_CATG'y
Cuts at: 1132 1229 2131 2789 4334 1132
Size: 97 902 658 1545 2229
Fragments arranged by size:
2229 1545 902 658 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5431
PciI A'CATG_T
Cuts at: 2127 4330 2127
Size: 2203 3228
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2575 1108
Size: 1467 3964
PfoI T'CCnGG_A
Cuts at: 2187 2187
Size: 5431
PmeI GTTT'AAAC
Cuts at: 2735 2735
Size: 5431
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3500 3532 5013 5045 3500
Size: 32 1481 32 3886
Fragments arranged by size:
3886 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2416 2416
Size: 5431
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5431
PsiI TTA'TAA
Cuts at: 3001 3001
Size: 5431
PstI C_TGCA'G
Cuts at: 830 2730 2783 830
Size: 1900 53 3478
Fragments arranged by size:
3478 1900 53
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5431
PvuII CAG'CTG
Cuts at: 2140 2140
Size: 5431
SacII CC_GC'GG
Cuts at: 2018 2018
Size: 5431
SalI G'TCGA_C
Cuts at: 1936 2773 1936
Size: 837 4594
SanDI GG'GwC_CC
Cuts at: 2416 2416
Size: 5431
SapI GCTCTTCn'nnn_
Cuts at: 2187 2187
Size: 5431
SbfI CC_TGCA'GG
Cuts at: 2783 2783
Size: 5431
ScaI AGT'ACT
Cuts at: 3679 5391 3679
Size: 1712 3719
SfcI C'TryA_G
Cuts at: 826 1045 2726 2779 3914 4595 4786 826
Size: 219 1681 53 1135 681 191 1471
Fragments arranged by size:
1681 1471 1135 681 219 191 53
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5431
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5431
SmaI CCC'GGG
Cuts at: 1872 2090 2228 2758 1872
Size: 218 138 530 4545
Fragments arranged by size:
4545 530 218 138
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2288 3535 4436 4698 820
Size: 197 926 345 1247 901 262 1553
Fragments arranged by size:
1553 1247 926 901 345 262 197
SnaBI TAC'GTA
Cuts at: 493 3222 493
Size: 2729 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5431
SphI G_CATG'C
Cuts at: 1132 2789 1132
Size: 1657 3774
SspI AAT'ATT
Cuts at: 5 52 3355 5
Size: 47 3303 2081
Fragments arranged by size:
3303 2081 47
StuI AGG'CCT
Cuts at: 1517 2396 1517
Size: 879 4552
StyI C'CwwG_G
Cuts at: 513 1065 2376 2870 513
Size: 552 1311 494 3074
Fragments arranged by size:
3074 1311 552 494
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3458 3475 3628 3813 4258 325
Size: 1255 1878 17 153 185 445 1498
Fragments arranged by size:
1878 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2382 3677 5389
Size: 274 80 33 51 1443 405 1295 1712
Cuts at: 5389 96
Size: 138
Fragments arranged by size:
1712 1443 1295 405 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2463 2495 2527 5391 5423 2463
Size: 32 32 2864 32 2471
Fragments arranged by size:
2864 2471 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5431
XbaI T'CTAG_A
Cuts at: 2767 2767
Size: 5431
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5431
XmaI C'CCGG_G
Cuts at: 1870 2088 2226 2756 1870
Size: 218 138 530 4545
Fragments arranged by size:
4545 530 218 138
XmnI GAAnn'nnTTC
Cuts at: 2327 3560 2327
Size: 1233 4198
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3239 276
Size: 53 83 186 936 1705 2468
Fragments arranged by size:
2468 1705 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacII SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacI SexAI SfiI SfoI SrfI
SwaI XcmI