Restriction map for
pFN21AE1732
(Circular) (Six-base) MAPSORT of: pFN21AE1732.seq Check: 6397 from: 1 to: 5425
pFN21AE1732 5425 bp
With 182 enzymes: SgfI *
July 6, 2012 17:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2419 2579 2419
Size: 160 5265
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3235 278
Size: 53 83 186 936 1699 2468
Fragments arranged by size:
2468 1699 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2768 1937
Size: 831 4594
Acc65I G'GTAC_C
Cuts at: 2746 2746
Size: 5425
AclI AA'CG_TT
Cuts at: 1563 3552 3925 1563
Size: 1989 373 3063
Fragments arranged by size:
3063 1989 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3488 4872 5140 1632
Size: 1856 1384 268 1917
Fragments arranged by size:
1917 1856 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5228
AflIII A'CryG_T
Cuts at: 1203 4324 5301 1203
Size: 3121 977 1327
Fragments arranged by size:
3121 1327 977
AgeI A'CCGG_T
Cuts at: 2219 4256 2219
Size: 2037 3388
AhdI GACnn_n'nnGTC
Cuts at: 4154 4154
Size: 5425
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5425
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5425
AlwNI CAG_nnn'CTG
Cuts at: 2303 3168 4740 2303
Size: 865 1572 2988
Fragments arranged by size:
2988 1572 865
ApaI G_GGCC'C
Cuts at: 2064 2151 2064
Size: 87 5338
ApaLI G'TGCA_C
Cuts at: 3482 4638 3482
Size: 1156 4269
ApoI r'AATT_y
Cuts at: 1493 2734 2960 3112 5221 1493
Size: 1241 226 152 2109 1697
Fragments arranged by size:
2109 1697 1241 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3979 160
Size: 872 2947 1606
Fragments arranged by size:
2947 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5425
AvaI C'yCGr_G
Cuts at: 1870 1943 2065 2750 1870
Size: 73 122 685 4545
Fragments arranged by size:
4545 685 122 73
BamHI G'GATC_C
Cuts at: 2755 3122 2755
Size: 367 5058
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2746 4201 618
Size: 325 217 627 959 1455 1842
Fragments arranged by size:
1842 1455 959 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2064 2151 2744 1391
Size: 673 87 593 4072
Fragments arranged by size:
4072 673 593 87
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4454
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3616 3650 1903
Size: 34 1679 34 3678
Fragments arranged by size:
3678 1679 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3317 4527 3317
Size: 1210 4215
BclI T'GATC_A
Cuts at: 1548 2613 1548
Size: 1065 4360
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2039 2185 4036
Size: 107 122 71 631 605 367 146 1851
Cuts at: 4036 136
Size: 1525
Fragments arranged by size:
1851 1525 631 605 367 146 122 107
71
BglII A'GATC_T
Cuts at: 5420 5420
Size: 5425
BlpI GC'TnA_GC
Cuts at: 2842 2842
Size: 5425
Bme1580I G_kGCm'C
Cuts at: 1790 2064 2151 3486 4642 1790
Size: 274 87 1335 1156 2573
Fragments arranged by size:
2573 1335 1156 274 87
BmgBI CAC'GTC
Cuts at: 1318 2197 1318
Size: 879 4546
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2324 2641 3156
Size: 416 53 83 399 24 907 317 515
Cuts at: 3156 4114 442
Size: 958 1753
Fragments arranged by size:
1753 958 907 515 416 399 317 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5425
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4085 1239
Size: 2846 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3550 4415 4713 3550
Size: 865 298 4262
Fragments arranged by size:
4262 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2636 4088 882
Size: 617 1137 1452 2219
Fragments arranged by size:
2219 1452 1137 617
BsaAI yAC'GTr
Cuts at: 493 1204 3216 493
Size: 711 2012 2702
Fragments arranged by size:
2702 2012 711
BsaBI GATnn'nnATC
Cuts at: 5419 5419
Size: 5425
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3232 3614 4249 275
Size: 53 83 186 936 1699 382 635 1451
Fragments arranged by size:
1699 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2219 3858 4256 4530 4677 5088 2219
Size: 1639 398 274 147 411 2556
Fragments arranged by size:
2556 1639 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2219 2249 2427 2457 1178
Size: 30 165 30 816 30 178 30 4146
Fragments arranged by size:
4146 816 178 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2258 2621 1186
Size: 1072 363 3990
Fragments arranged by size:
3990 1072 363
BseYI C'CCAG_C
Cuts at: 2200 2378 2555 4628 5124 2200
Size: 178 177 2073 496 2501
Fragments arranged by size:
2501 2073 496 178 177
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4852
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2184 2875 3636 4664 1540
Size: 275 186 183 691 761 1028 2301
Fragments arranged by size:
2301 1028 761 691 275 186 183
BsiHKAI G_wGCw'C
Cuts at: 2580 3486 3571 4642 2580
Size: 906 85 1071 3363
Fragments arranged by size:
3363 1071 906 85
BsmI GAATG_Cn'
Cuts at: 2936 3029 2936
Size: 93 5332
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2151 2580 2744 3486 3571 4642
Size: 399 274 87 429 164 742 85 1071
Cuts at: 4642 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 429 399 274 164 87
85
BspEI T'CCGG_A
Cuts at: 5088 5088
Size: 5425
BspHI T'CATG_A
Cuts at: 3314 5044 3314
Size: 1730 3695
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2419 2579 2780 844
Size: 1575 160 201 3489
Fragments arranged by size:
3489 1575 201 160
BsrBI CCG'CTC
Cuts at: 1394 1823 2054 3312 1394
Size: 429 231 1258 3507
Fragments arranged by size:
3507 1258 429 231
BsrDI GCAATG_nn'
Cuts at: 65 1248 3920 4094 65
Size: 1183 2672 174 1396
Fragments arranged by size:
2672 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2219 2355 2676 4069 4256 1700
Size: 110 409 136 321 1393 187 2869
Fragments arranged by size:
2869 1393 409 321 187 136 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5425
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5425
BssSI C'ACGA_G
Cuts at: 3485 4497 3485
Size: 1012 4413
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5425
BstBI TT'CG_AA
Cuts at: 4229 4229
Size: 5425
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5425
BstYI r'GATC_y
Cuts at: 1972 2755 3122 3507 3524 4976 5062 5074 5420
Size: 783 367 385 17 1452 86 12 346
Cuts at: 5420 1972
Size: 1977
Fragments arranged by size:
1977 1452 783 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2241 2423 2241
Size: 182 5243
BtgI C'CryG_G
Cuts at: 513 1065 2439 513
Size: 552 1374 3499
Fragments arranged by size:
3499 1374 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4686
Fragments arranged by size:
4686 683 56
BtsI GCAGTG_nn'
Cuts at: 2941 3735 3755 2941
Size: 794 20 4611
Fragments arranged by size:
4611 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5425
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5390
DraI TTT'AAA
Cuts at: 2729 2729
Size: 5425
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2468 4432 809
Size: 507 1152 1964 1802
Fragments arranged by size:
1964 1802 1152 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2872 3761 5390
Size: 54 1425 50 275 18 1042 889 1629
Cuts at: 5390 8
Size: 43
Fragments arranged by size:
1629 1425 1042 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2872 1537
Size: 275 1060 4090
Fragments arranged by size:
4090 1060 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3355 1353
Size: 2002 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2408 2618 4012 4386 4532 387
Size: 1064 138 819 210 1394 374 146 1280
Fragments arranged by size:
1394 1280 1064 819 374 210 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3488 4085 4872 5140 1239
Size: 393 1856 597 787 268 1524
Fragments arranged by size:
1856 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2426 2426
Size: 5425
EcoO109I rG'GnC_Cy
Cuts at: 2060 2061 2147 2362 2060
Size: 1 86 215 5123
Fragments arranged by size:
5123 215 86 1
EcoRI G'AATT_C
Cuts at: 2734 3112 2734
Size: 378 5047
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2281 2281
Size: 5425
FspI TGC'GCA
Cuts at: 3931 3931
Size: 5425
HaeII r_GCGC'y
Cuts at: 1678 2386 4572 1678
Size: 708 2186 2531
Fragments arranged by size:
2531 2186 708
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2279 2311 2633 2665 4066
Size: 32 135 32 707 32 322 32 1401
Cuts at: 4066 4098 4140 4172 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1401 707 322 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2473 2769 3015 669
Size: 988 281 535 296 246 3079
Fragments arranged by size:
3079 988 535 296 281 246
HindIII A'AGCT_T
Cuts at: 748 2524 748
Size: 1776 3649
HpaI GTT'AAC
Cuts at: 3015 3015
Size: 5425
KpnI G_GTAC'C
Cuts at: 2750 2750
Size: 5425
MfeI C'AATT_G
Cuts at: 3024 3024
Size: 5425
MluI A'CGCG_T
Cuts at: 5301 5301
Size: 5425
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2106 4539 4723 1305
Size: 801 2433 184 2007
Fragments arranged by size:
2433 2007 801 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3603
Fragments arranged by size:
3603 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2637 3383 3742 3901 518
Size: 648 843 628 746 359 159 2042
Fragments arranged by size:
2042 843 746 648 628 359 159
MspA1I CmG'CkG
Cuts at: 1606 2164 2212 2715 2841 3518 4666 4911 1606
Size: 558 48 503 126 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 558 503 245 126 48
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4873
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5425
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5425
NotI GC'GGCC_GC
Cuts at: 2872 2872
Size: 5425
NspI r_CATG'y
Cuts at: 1132 1229 2783 4328 1132
Size: 97 1554 1545 2229
Fragments arranged by size:
2229 1554 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2144 1348
Size: 796 4629
PciI A'CATG_T
Cuts at: 4324 4324
Size: 5425
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2077 1108
Size: 969 4456
PmeI GTTT'AAAC
Cuts at: 2729 2729
Size: 5425
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3494 3526 5007 5039 3494
Size: 32 1481 32 3880
Fragments arranged by size:
3880 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2362 2362
Size: 5425
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5425
PsiI TTA'TAA
Cuts at: 2995 2995
Size: 5425
PspOMI G'GGCC_C
Cuts at: 2060 2147 2060
Size: 87 5338
PstI C_TGCA'G
Cuts at: 830 2169 2211 2777 830
Size: 1339 42 566 3478
Fragments arranged by size:
3478 1339 566 42
PvuI CG_AT'CG
Cuts at: 2001 2184 2001
Size: 183 5242
SalI G'TCGA_C
Cuts at: 1936 2767 1936
Size: 831 4594
SbfI CC_TGCA'GG
Cuts at: 2777 2777
Size: 5425
ScaI AGT'ACT
Cuts at: 3673 5385 3673
Size: 1712 3713
SfcI C'TryA_G
Cuts at: 826 1045 2165 2207 2710 2773 3908 4589 4780
Size: 219 1120 42 503 63 1135 681 191
Cuts at: 4780 826
Size: 1471
Fragments arranged by size:
1471 1135 1120 681 503 219 191 63
42
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5425
SgrAI Cr'CCGG_yG
Cuts at: 1700 2219 1700
Size: 519 4906
SmaI CCC'GGG
Cuts at: 1872 2752 1872
Size: 880 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3529 4430 4692 820
Size: 197 926 1586 901 262 1553
Fragments arranged by size:
1586 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3216 493
Size: 2723 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5425
SphI G_CATG'C
Cuts at: 1132 2783 1132
Size: 1651 3774
SspI AAT'ATT
Cuts at: 5 52 3349 5
Size: 47 3297 2081
Fragments arranged by size:
3297 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5425
StyI C'CwwG_G
Cuts at: 513 1065 2864 513
Size: 552 1799 3074
Fragments arranged by size:
3074 1799 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3452 3469 3622 3807 4252 325
Size: 1255 1872 17 153 185 445 1498
Fragments arranged by size:
1872 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3671 5383 96
Size: 274 80 33 51 1443 1694 1712 138
Fragments arranged by size:
1712 1694 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2206 2238 2427 2459 5385 5417 2206
Size: 32 189 32 2926 32 2214
Fragments arranged by size:
2926 2214 189 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2047 2267 2647 1316
Size: 731 220 380 4094
Fragments arranged by size:
4094 731 380 220
XbaI T'CTAG_A
Cuts at: 2761 2761
Size: 5425
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5425
XmaI C'CCGG_G
Cuts at: 1870 2750 1870
Size: 880 4545
XmnI GAAnn'nnTTC
Cuts at: 3554 3554
Size: 5425
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3233 276
Size: 53 83 186 936 1699 2468
Fragments arranged by size:
2468 1699 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI