Restriction map for
pFN21AE1731
(Circular) (Six-base) MAPSORT of: pFN21AE1731.seq Check: 2826 from: 1 to: 5422
pFN21AE1731 5422 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3232 278
Size: 53 83 186 936 1696 2468
Fragments arranged by size:
2468 1696 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2109 2765 1937
Size: 172 656 4594
Fragments arranged by size:
4594 656 172
Acc65I G'GTAC_C
Cuts at: 2289 2743 2289
Size: 454 4968
AclI AA'CG_TT
Cuts at: 1563 3549 3922 1563
Size: 1986 373 3063
Fragments arranged by size:
3063 1986 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3485 4869 5137 1632
Size: 1853 1384 268 1917
Fragments arranged by size:
1917 1853 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5225
AflIII A'CryG_T
Cuts at: 1203 2466 4321 5298 1203
Size: 1263 1855 977 1327
Fragments arranged by size:
1855 1327 1263 977
AgeI A'CCGG_T
Cuts at: 4253 4253
Size: 5422
AhdI GACnn_n'nnGTC
Cuts at: 4151 4151
Size: 5422
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5422
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5422
AlwNI CAG_nnn'CTG
Cuts at: 3165 4737 3165
Size: 1572 3850
ApaI G_GGCC'C
Cuts at: 2066 2434 2066
Size: 368 5054
ApaLI G'TGCA_C
Cuts at: 3479 4635 3479
Size: 1156 4266
ApoI r'AATT_y
Cuts at: 1493 2731 2957 3109 5218 1493
Size: 1238 226 152 2109 1697
Fragments arranged by size:
2109 1697 1238 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3976 160
Size: 872 2944 1606
Fragments arranged by size:
2944 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5422
AvaI C'yCGr_G
Cuts at: 1870 1943 2182 2747 1870
Size: 73 239 565 4545
Fragments arranged by size:
4545 565 239 73
BamHI G'GATC_C
Cuts at: 2364 2752 3119 2364
Size: 388 367 4667
Fragments arranged by size:
4667 388 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2128 2136 2289 2743 4198
Size: 325 217 627 341 8 153 454 1455
Cuts at: 4198 618
Size: 1842
Fragments arranged by size:
1842 1455 627 454 341 325 217 153
8
BanII G_rGCy'C
Cuts at: 1391 2046 2066 2074 2434 2654 2670 2699 2741
Size: 655 20 8 360 220 16 29 42
Cuts at: 2741 1391
Size: 4072
Fragments arranged by size:
4072 655 360 220 42 29 20 16
8
BbeI G_GCGC'C
Cuts at: 2132 2140 2132
Size: 8 5414
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4451
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3613 3647 1903
Size: 34 1676 34 3678
Fragments arranged by size:
3678 1676 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3314 4524 3314
Size: 1210 4212
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5422
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2050 2103 2632
Size: 107 122 71 631 605 378 53 529
Cuts at: 2632 4033 136
Size: 1401 1525
Fragments arranged by size:
1525 1401 631 605 529 378 122 107
71 53
BglII A'GATC_T
Cuts at: 5417 5417
Size: 5422
BlpI GC'TnA_GC
Cuts at: 2030 2839 2030
Size: 809 4613
Bme1580I G_kGCm'C
Cuts at: 1790 2066 2434 3483 4639 1790
Size: 276 368 1049 1156 2573
Fragments arranged by size:
2573 1156 1049 368 276
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5422
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2702 3153 4111
Size: 416 53 83 399 24 1285 451 958
Cuts at: 4111 442
Size: 1753
Fragments arranged by size:
1753 1285 958 451 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5422
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2060 2156 2228 2684 2726 4082 1239
Size: 821 96 72 456 42 1356 2579
Fragments arranged by size:
2579 1356 821 456 96 72 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3547 4412 4710 3547
Size: 865 298 4259
Fragments arranged by size:
4259 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2279 4085 882
Size: 617 780 1806 2219
Fragments arranged by size:
2219 1806 780 617
BsaAI yAC'GTr
Cuts at: 493 1204 2388 2467 3213 493
Size: 711 1184 79 746 2702
Fragments arranged by size:
2702 1184 746 711 79
BsaBI GATnn'nnATC
Cuts at: 5416 5416
Size: 5422
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2106 2129 2137 3229
Size: 53 83 186 936 573 23 8 1092
Cuts at: 3229 3611 4246 275
Size: 382 635 1451
Fragments arranged by size:
1451 1092 936 635 573 382 186 83
53 23 8
BsaWI w'CCGG_w
Cuts at: 2406 3855 4253 4527 4674 5085 2406
Size: 1449 398 274 147 411 2743
Fragments arranged by size:
2743 1449 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5197
Fragments arranged by size:
5197 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5422
BseYI C'CCAG_C
Cuts at: 2374 2444 2700 4625 5121 2374
Size: 70 256 1925 496 2675
Fragments arranged by size:
2675 1925 496 256 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2162 1163
Size: 573 426 4423
Fragments arranged by size:
4423 573 426
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2098 2191 2872 3633 4661 1540
Size: 275 186 97 93 681 761 1028 2301
Fragments arranged by size:
2301 1028 761 681 275 186 97 93
BsiHKAI G_wGCw'C
Cuts at: 2118 2334 3483 3568 4639 2118
Size: 216 1149 85 1071 2901
Fragments arranged by size:
2901 1149 1071 216 85
BsmI GAATG_Cn'
Cuts at: 2933 3026 2933
Size: 93 5329
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2066 2074 2118 2334 2434 2654
Size: 399 256 20 8 44 216 100 220
Cuts at: 2654 2670 2699 2741 3483 3568 4639 1391
Size: 16 29 42 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 256 220 216 100
85 44 42 29 20 16 8
BspEI T'CCGG_A
Cuts at: 5085 5085
Size: 5422
BspHI T'CATG_A
Cuts at: 3311 5041 3311
Size: 1730 3692
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2777 844
Size: 1933 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2187 2362 3309 1394
Size: 429 364 175 947 3507
Fragments arranged by size:
3507 947 429 364 175
BsrDI GCAATG_nn'
Cuts at: 65 1248 3917 4091 65
Size: 1183 2669 174 1396
Fragments arranged by size:
2669 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2436 2487 4066 4253 1700
Size: 110 626 51 1579 187 2869
Fragments arranged by size:
2869 1579 626 187 110 51
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5422
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5422
BssSI C'ACGA_G
Cuts at: 2369 2567 3482 4494 2369
Size: 198 915 1012 3297
Fragments arranged by size:
3297 1012 915 198
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5422
BstBI TT'CG_AA
Cuts at: 4226 4226
Size: 5422
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5422
BstYI r'GATC_y
Cuts at: 1972 2364 2752 3119 3504 3521 4973 5059 5071
Size: 392 388 367 385 17 1452 86 12
Cuts at: 5071 5417 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 392 388 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2098 513
Size: 552 939 94 3837
Fragments arranged by size:
3837 939 552 94
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2100 487
Size: 683 56 874 3809
Fragments arranged by size:
3809 874 683 56
BtsI GCAGTG_nn'
Cuts at: 2250 2938 3732 3752 2250
Size: 688 794 20 3920
Fragments arranged by size:
3920 794 688 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5422
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5387
DraI TTT'AAA
Cuts at: 2726 2726
Size: 5422
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4429 809
Size: 507 3113 1802
Fragments arranged by size:
3113 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2095 2188 2262
Size: 54 1425 50 275 18 265 93 74
Cuts at: 2262 2295 2869 3758 5387 8
Size: 33 574 889 1629 43
Fragments arranged by size:
1629 1425 889 574 275 265 93 74
54 50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 2095 2188 2869 1537
Size: 275 283 93 681 4090
Fragments arranged by size:
4090 681 283 275 93
EarI CTCTTCn'nnn_
Cuts at: 1353 2282 3352 1353
Size: 929 1070 3423
Fragments arranged by size:
3423 1070 929
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4009 4383 4529 387
Size: 1064 138 2420 374 146 1280
Fragments arranged by size:
2420 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2156 2228 2684 2726 3485 4082
Size: 393 428 96 72 456 42 759 597
Cuts at: 4082 4869 5137 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 759 597 456 428 393 268
96 72 42
EcoO109I rG'GnC_Cy
Cuts at: 2063 2430 2063
Size: 367 5055
EcoRI G'AATT_C
Cuts at: 2731 3109 2731
Size: 378 5044
FseI GG_CCGG'CC
Cuts at: 2491 2491
Size: 5422
FspI TGC'GCA
Cuts at: 2718 3928 2718
Size: 1210 4212
FspAI rTGC'GCAy
Cuts at: 2718 2718
Size: 5422
HaeII r_GCGC'y
Cuts at: 1678 2132 2140 2220 2343 2451 4569 1678
Size: 454 8 80 123 108 2118 2531
Fragments arranged by size:
2531 2118 454 123 108 80 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4063 4095 4137 4169 1373
Size: 32 135 32 2491 32 42 32 2626
Fragments arranged by size:
2626 2491 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2197 2766 3012 669
Size: 988 281 259 569 246 3079
Fragments arranged by size:
3079 988 569 281 259 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5422
HpaI GTT'AAC
Cuts at: 3012 3012
Size: 5422
KasI G'GCGC_C
Cuts at: 2128 2136 2128
Size: 8 5414
KpnI G_GTAC'C
Cuts at: 2293 2747 2293
Size: 454 4968
MfeI C'AATT_G
Cuts at: 3021 3021
Size: 5422
MluI A'CGCG_T
Cuts at: 5298 5298
Size: 5422
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4536 4720 1305
Size: 3231 184 2007
Fragments arranged by size:
3231 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2264 2297 10
Size: 54 1425 343 432 33 3135
Fragments arranged by size:
3135 1425 432 343 54 33
MslI CAynn'nnrTG
Cuts at: 518 1166 3380 3739 3898 518
Size: 648 2214 359 159 2042
Fragments arranged by size:
2214 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2060 2176 2704 2838 3515 4663 4908 1606
Size: 454 116 528 134 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 528 454 245 134 116
NaeI GCC'GGC
Cuts at: 2438 2489 2438
Size: 51 5371
NarI GG'CG_CC
Cuts at: 2129 2137 2129
Size: 8 5414
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3931
Fragments arranged by size:
3931 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5422
NgoMIV G'CCGG_C
Cuts at: 2436 2487 2436
Size: 51 5371
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5422
NotI GC'GGCC_GC
Cuts at: 2188 2869 2188
Size: 681 4741
NspI r_CATG'y
Cuts at: 1132 1229 2780 4325 1132
Size: 97 1551 1545 2229
Fragments arranged by size:
2229 1551 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2066 1348
Size: 718 4704
PciI A'CATG_T
Cuts at: 4321 4321
Size: 5422
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2374 2572 1108
Size: 1266 198 3958
Fragments arranged by size:
3958 1266 198
PmeI GTTT'AAAC
Cuts at: 2726 2726
Size: 5422
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3491 3523 5004 5036 3491
Size: 32 1481 32 3877
Fragments arranged by size:
3877 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5422
PsiI TTA'TAA
Cuts at: 2992 2992
Size: 5422
PspOMI G'GGCC_C
Cuts at: 2062 2430 2062
Size: 368 5054
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2204 2236 2204
Size: 32 5390
PstI C_TGCA'G
Cuts at: 830 2774 830
Size: 1944 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5422
PvuII CAG'CTG
Cuts at: 2176 2704 2176
Size: 528 4894
SalI G'TCGA_C
Cuts at: 1936 2764 1936
Size: 828 4594
SbfI CC_TGCA'GG
Cuts at: 2774 2774
Size: 5422
ScaI AGT'ACT
Cuts at: 3670 5382 3670
Size: 1712 3710
SfcI C'TryA_G
Cuts at: 826 1045 2221 2335 2585 2770 3905 4586 4777
Size: 219 1176 114 250 185 1135 681 191
Cuts at: 4777 826
Size: 1471
Fragments arranged by size:
1471 1176 1135 681 250 219 191 185
114
SfoI GGC'GCC
Cuts at: 2130 2138 2130
Size: 8 5414
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5422
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5422
SmaI CCC'GGG
Cuts at: 1872 2749 1872
Size: 877 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3526 4427 4689 820
Size: 197 926 1583 901 262 1553
Fragments arranged by size:
1583 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2388 3213 493
Size: 1895 825 2702
Fragments arranged by size:
2702 1895 825
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5422
SphI G_CATG'C
Cuts at: 1132 2780 1132
Size: 1648 3774
SspI AAT'ATT
Cuts at: 5 52 3346 5
Size: 47 3294 2081
Fragments arranged by size:
3294 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5422
StyI C'CwwG_G
Cuts at: 513 1065 2004 2119 2861 513
Size: 552 939 115 742 3074
Fragments arranged by size:
3074 939 742 552 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3449 3466 3619 3804 4249 325
Size: 1255 1869 17 153 185 445 1498
Fragments arranged by size:
1869 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3668 5380 96
Size: 274 80 33 51 1443 1691 1712 138
Fragments arranged by size:
1712 1691 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5382 5414 5382
Size: 32 5390
Tth111I GACn'n_nGTC
Cuts at: 1316 2413 1316
Size: 1097 4325
XbaI T'CTAG_A
Cuts at: 2758 2758
Size: 5422
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5422
XmaI C'CCGG_G
Cuts at: 1870 2747 1870
Size: 877 4545
XmnI GAAnn'nnTTC
Cuts at: 3551 3551
Size: 5422
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3230 276
Size: 53 83 186 936 1696 2468
Fragments arranged by size:
2468 1696 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FseI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI NruI NsiI PacI PfoI
PmlI PpuMI PspXI RsrII SacI SacII SanDI SapI
SexAI SfiI SrfI SwaI XcmI