Restriction map for
pFN21AE1726
(Circular) (Six-base) MAPSORT of: pFN21AE1726.seq Check: 2464 from: 1 to: 5413
pFN21AE1726 5413 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3223 278
Size: 53 83 186 936 1687 2468
Fragments arranged by size:
2468 1687 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2756 1937
Size: 819 4594
Acc65I G'GTAC_C
Cuts at: 2734 2734
Size: 5413
AclI AA'CG_TT
Cuts at: 1563 3540 3913 1563
Size: 1977 373 3063
Fragments arranged by size:
3063 1977 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2694 3476 4860 5128 1632
Size: 1062 782 1384 268 1917
Fragments arranged by size:
1917 1384 1062 782 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5216
AflIII A'CryG_T
Cuts at: 1203 4312 5289 1203
Size: 3109 977 1327
Fragments arranged by size:
3109 1327 977
AgeI A'CCGG_T
Cuts at: 2517 4244 2517
Size: 1727 3686
AhdI GACnn_n'nnGTC
Cuts at: 4142 4142
Size: 5413
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5413
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5413
AlwNI CAG_nnn'CTG
Cuts at: 3156 4728 3156
Size: 1572 3841
ApaLI G'TGCA_C
Cuts at: 3470 4626 3470
Size: 1156 4257
ApoI r'AATT_y
Cuts at: 1493 2722 2948 3100 5209 1493
Size: 1229 226 152 2109 1697
Fragments arranged by size:
2109 1697 1229 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2606 3967 160
Size: 872 1574 1361 1606
Fragments arranged by size:
1606 1574 1361 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5413
AvaI C'yCGr_G
Cuts at: 1870 1943 2738 1870
Size: 73 795 4545
Fragments arranged by size:
4545 795 73
BamHI G'GATC_C
Cuts at: 2180 2743 3110 2180
Size: 563 367 4483
Fragments arranged by size:
4483 563 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2734 4189 618
Size: 325 217 627 947 1455 1842
Fragments arranged by size:
1842 1455 947 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2304 2732 1391
Size: 913 428 4072
Fragments arranged by size:
4072 913 428
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2379 928
Size: 971 480 3962
Fragments arranged by size:
3962 971 480
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3604 3638 1903
Size: 34 1667 34 3678
Fragments arranged by size:
3678 1667 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3305 4515 3305
Size: 1210 4203
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5413
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2022 4024 136
Size: 107 122 71 631 605 350 2002 1525
Fragments arranged by size:
2002 1525 631 605 350 122 107 71
BglII A'GATC_T
Cuts at: 5408 5408
Size: 5413
BlpI GC'TnA_GC
Cuts at: 2830 2830
Size: 5413
Bme1580I G_kGCm'C
Cuts at: 1790 3474 4630 1790
Size: 1684 1156 2573
Fragments arranged by size:
2573 1684 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5413
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2309 3144 4102
Size: 416 53 83 399 24 892 835 958
Cuts at: 4102 442
Size: 1753
Fragments arranged by size:
1753 958 892 835 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5413
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2505 2505
Size: 5413
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2472 4073 1239
Size: 1233 1601 2579
Fragments arranged by size:
2579 1601 1233
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3538 4403 4701 3538
Size: 865 298 4250
Fragments arranged by size:
4250 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2344 4076 882
Size: 617 845 1732 2219
Fragments arranged by size:
2219 1732 845 617
BsaAI yAC'GTr
Cuts at: 493 1204 2056 3204 493
Size: 711 852 1148 2702
Fragments arranged by size:
2702 1148 852 711
BsaBI GATnn'nnATC
Cuts at: 5407 5407
Size: 5413
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2422 3220 3602 4237
Size: 53 83 186 936 889 798 382 635
Cuts at: 4237 275
Size: 1451
Fragments arranged by size:
1451 936 889 798 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2517 3846 4244 4518 4665 5076 2517
Size: 1329 398 274 147 411 2854
Fragments arranged by size:
2854 1329 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5188
Fragments arranged by size:
5188 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2635 2651 1186
Size: 1449 16 3948
Fragments arranged by size:
3948 1449 16
BseYI C'CCAG_C
Cuts at: 4616 5112 4616
Size: 496 4917
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4840
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2863 3624 4652 1540
Size: 275 186 862 761 1028 2301
Fragments arranged by size:
2301 1028 862 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3474 3559 4630 3474
Size: 85 1071 4257
Fragments arranged by size:
4257 1071 85
BsmI GAATG_Cn'
Cuts at: 2924 3017 2924
Size: 93 5320
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2304 2732 3474 3559 4630 1391
Size: 399 514 428 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 514 428 399 85
BspEI T'CCGG_A
Cuts at: 5076 5076
Size: 5413
BspHI T'CATG_A
Cuts at: 3302 5032 3302
Size: 1730 3683
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2389 2768 844
Size: 1545 379 3489
Fragments arranged by size:
3489 1545 379
BsrBI CCG'CTC
Cuts at: 1394 1823 2547 3300 1394
Size: 429 724 753 3507
Fragments arranged by size:
3507 753 724 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3908 4082 65
Size: 1183 2660 174 1396
Fragments arranged by size:
2660 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2126 2517 4057 4244 1700
Size: 110 316 391 1540 187 2869
Fragments arranged by size:
2869 1540 391 316 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5413
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5413
BssSI C'ACGA_G
Cuts at: 3473 4485 3473
Size: 1012 4401
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5413
BstBI TT'CG_AA
Cuts at: 4217 4217
Size: 5413
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5413
BstYI r'GATC_y
Cuts at: 1972 2180 2743 3110 3495 3512 4964 5050 5062
Size: 208 563 367 385 17 1452 86 12
Cuts at: 5062 5408 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 563 385 367 346 208 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2380 513
Size: 552 1315 3546
Fragments arranged by size:
3546 1315 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4674
Fragments arranged by size:
4674 683 56
BtsI GCAGTG_nn'
Cuts at: 2429 2929 3723 3743 2429
Size: 500 794 20 4099
Fragments arranged by size:
4099 794 500 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5413
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5378
DraI TTT'AAA
Cuts at: 2717 2717
Size: 5413
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4420 809
Size: 507 3104 1802
Fragments arranged by size:
3104 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2023 2383 2860
Size: 54 1425 50 275 18 193 360 477
Cuts at: 2860 3749 5378 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 477 360 275 193 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2860 1537
Size: 275 1048 4090
Fragments arranged by size:
4090 1048 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2474 2646 3343 1353
Size: 1121 172 697 3423
Fragments arranged by size:
3423 1121 697 172
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2060 2145 4000 4374 4520 387
Size: 1064 138 471 85 1855 374 146 1280
Fragments arranged by size:
1855 1280 1064 471 374 146 138 85
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2472 2694 3476 4073 4860 5128 1239
Size: 393 840 222 782 597 787 268 1524
Fragments arranged by size:
1524 840 787 782 597 393 268 222
EcoO109I rG'GnC_Cy
Cuts at: 2231 2262 2231
Size: 31 5382
EcoRI G'AATT_C
Cuts at: 2722 3100 2722
Size: 378 5035
FspI TGC'GCA
Cuts at: 3919 3919
Size: 5413
HaeII r_GCGC'y
Cuts at: 1678 2143 4560 1678
Size: 465 2417 2531
Fragments arranged by size:
2531 2417 465
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4054 4086 4128 4160 1373
Size: 32 135 32 2482 32 42 32 2626
Fragments arranged by size:
2626 2482 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2560 2757 3003 669
Size: 988 281 622 197 246 3079
Fragments arranged by size:
3079 988 622 281 246 197
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5413
HpaI GTT'AAC
Cuts at: 3003 3003
Size: 5413
KpnI G_GTAC'C
Cuts at: 2738 2738
Size: 5413
MfeI C'AATT_G
Cuts at: 3012 3012
Size: 5413
MluI A'CGCG_T
Cuts at: 5289 5289
Size: 5413
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4527 4711 1305
Size: 3222 184 2007
Fragments arranged by size:
3222 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2025 2385 10
Size: 54 1425 343 193 360 3038
Fragments arranged by size:
3038 1425 360 343 193 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3371 3730 3889 518
Size: 648 2205 359 159 2042
Fragments arranged by size:
2205 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2021 2672 2829 3506 4654 4899 1606
Size: 415 651 157 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 651 415 245 157
NaeI GCC'GGC
Cuts at: 2128 2128
Size: 5413
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4861
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5413
NgoMIV G'CCGG_C
Cuts at: 2126 2126
Size: 5413
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5413
NotI GC'GGCC_GC
Cuts at: 2860 2860
Size: 5413
NspI r_CATG'y
Cuts at: 1132 1229 2771 4316 1132
Size: 97 1542 1545 2229
Fragments arranged by size:
2229 1545 1542 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5413
PciI A'CATG_T
Cuts at: 4312 4312
Size: 5413
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5413
PmeI GTTT'AAAC
Cuts at: 2717 2717
Size: 5413
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3482 3514 4995 5027 3482
Size: 32 1481 32 3868
Fragments arranged by size:
3868 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2262 2262
Size: 5413
PshAI GACnn'nnGTC
Cuts at: 1613 2573 1613
Size: 960 4453
PsiI TTA'TAA
Cuts at: 2983 2983
Size: 5413
PstI C_TGCA'G
Cuts at: 830 2118 2439 2665 2765 830
Size: 1288 321 226 100 3478
Fragments arranged by size:
3478 1288 321 226 100
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5413
PvuII CAG'CTG
Cuts at: 2672 2672
Size: 5413
SalI G'TCGA_C
Cuts at: 1936 2755 1936
Size: 819 4594
SbfI CC_TGCA'GG
Cuts at: 2765 2765
Size: 5413
ScaI AGT'ACT
Cuts at: 3661 5373 3661
Size: 1712 3701
SfcI C'TryA_G
Cuts at: 826 1045 2114 2435 2661 2761 3896 4577 4768
Size: 219 1069 321 226 100 1135 681 191
Cuts at: 4768 826
Size: 1471
Fragments arranged by size:
1471 1135 1069 681 321 226 219 191
100
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5413
SgrAI Cr'CCGG_yG
Cuts at: 1700 2517 1700
Size: 817 4596
SmaI CCC'GGG
Cuts at: 1872 2740 1872
Size: 868 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3517 4418 4680 820
Size: 197 926 1574 901 262 1553
Fragments arranged by size:
1574 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3204 493
Size: 2711 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5413
SphI G_CATG'C
Cuts at: 1132 2771 1132
Size: 1639 3774
SspI AAT'ATT
Cuts at: 5 52 3337 5
Size: 47 3285 2081
Fragments arranged by size:
3285 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5413
StyI C'CwwG_G
Cuts at: 513 1065 2852 513
Size: 552 1787 3074
Fragments arranged by size:
3074 1787 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3440 3457 3610 3795 4240 325
Size: 1255 1860 17 153 185 445 1498
Fragments arranged by size:
1860 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3659 5371 96
Size: 274 80 33 51 1443 1682 1712 138
Fragments arranged by size:
1712 1682 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5373 5405 5373
Size: 32 5381
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5413
XbaI T'CTAG_A
Cuts at: 2749 2749
Size: 5413
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5413
XmaI C'CCGG_G
Cuts at: 1870 2738 1870
Size: 868 4545
XmnI GAAnn'nnTTC
Cuts at: 3542 3542
Size: 5413
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3221 276
Size: 53 83 186 936 1687 2468
Fragments arranged by size:
2468 1687 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI