Restriction map for
pFN21AE1725
(Circular) (Six-base) MAPSORT of: pFN21AE1725.seq Check: 4089 from: 1 to: 5410
pFN21AE1725 5410 bp
With 182 enzymes: SgfI *
January 30, 2014 17:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2653 2653
Size: 5410
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3220 278
Size: 53 83 186 936 1684 2468
Fragments arranged by size:
2468 1684 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2753 1937
Size: 816 4594
Acc65I G'GTAC_C
Cuts at: 2731 2731
Size: 5410
AclI AA'CG_TT
Cuts at: 1563 3537 3910 1563
Size: 1974 373 3063
Fragments arranged by size:
3063 1974 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2447 3473 4857 5125 1632
Size: 815 1026 1384 268 1917
Fragments arranged by size:
1917 1384 1026 815 268
AfeI AGC'GCT
Cuts at: 2246 2246
Size: 5410
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5213
AflIII A'CryG_T
Cuts at: 1203 4309 5286 1203
Size: 3106 977 1327
Fragments arranged by size:
3106 1327 977
AgeI A'CCGG_T
Cuts at: 4241 4241
Size: 5410
AhdI GACnn_n'nnGTC
Cuts at: 4139 4139
Size: 5410
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5410
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5410
AlwNI CAG_nnn'CTG
Cuts at: 2692 3153 4725 2692
Size: 461 1572 3377
Fragments arranged by size:
3377 1572 461
ApaLI G'TGCA_C
Cuts at: 3467 4623 3467
Size: 1156 4254
ApoI r'AATT_y
Cuts at: 1493 2719 2945 3097 5206 1493
Size: 1226 226 152 2109 1697
Fragments arranged by size:
2109 1697 1226 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3964 160
Size: 872 2932 1606
Fragments arranged by size:
2932 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5410
AvaI C'yCGr_G
Cuts at: 1870 1943 2068 2735 1870
Size: 73 125 667 4545
Fragments arranged by size:
4545 667 125 73
BamHI G'GATC_C
Cuts at: 2740 3107 2740
Size: 367 5043
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2017 2460 2613 2665 2731
Size: 325 217 627 230 443 153 52 66
Cuts at: 2731 4186 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 443 325 230 217 153
66 52
BanII G_rGCy'C
Cuts at: 1391 2033 2069 2211 2729 1391
Size: 642 36 142 518 4072
Fragments arranged by size:
4072 642 518 142 36
BbeI G_GCGC'C
Cuts at: 2021 2021
Size: 5410
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2354 928
Size: 971 455 3984
Fragments arranged by size:
3984 971 455
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3601 3635 1903
Size: 34 1664 34 3678
Fragments arranged by size:
3678 1664 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3302 4512 3302
Size: 1210 4200
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5410
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4021 136
Size: 107 122 71 631 605 2349 1525
Fragments arranged by size:
2349 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5405 5405
Size: 5410
BlpI GC'TnA_GC
Cuts at: 2063 2827 2063
Size: 764 4646
Bme1580I G_kGCm'C
Cuts at: 1790 2670 3471 4627 1790
Size: 880 801 1156 2573
Fragments arranged by size:
2573 1156 880 801
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5410
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2168 2673 3141
Size: 416 53 83 399 24 751 505 468
Cuts at: 3141 4099 442
Size: 958 1753
Fragments arranged by size:
1753 958 751 505 468 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5410
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2047 2318 2486 2546 4070 1239
Size: 808 271 168 60 1524 2579
Fragments arranged by size:
2579 1524 808 271 168 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2246 3535 4400 4698 2246
Size: 1289 865 298 2958
Fragments arranged by size:
2958 1289 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2396 4073 882
Size: 617 897 1677 2219
Fragments arranged by size:
2219 1677 897 617
BsaAI yAC'GTr
Cuts at: 493 1204 3201 493
Size: 711 1997 2702
Fragments arranged by size:
2702 1997 711
BsaBI GATnn'nnATC
Cuts at: 5404 5404
Size: 5410
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2018 3217 3599 4234
Size: 53 83 186 936 485 1199 382 635
Cuts at: 4234 275
Size: 1451
Fragments arranged by size:
1451 1199 936 635 485 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3843 4241 4515 4662 5073 3843
Size: 398 274 147 411 4180
Fragments arranged by size:
4180 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2459 2489 1178
Size: 30 165 30 1056 30 4099
Fragments arranged by size:
4099 1056 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2036 2107 2219 2479 2555 1186
Size: 850 71 112 260 76 4041
Fragments arranged by size:
4041 850 260 112 76 71
BseYI C'CCAG_C
Cuts at: 2273 2354 4613 5109 2273
Size: 81 2259 496 2574
Fragments arranged by size:
2574 2259 496 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2603 1163
Size: 573 867 3970
Fragments arranged by size:
3970 867 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2860 3621 4649 1540
Size: 275 186 859 761 1028 2301
Fragments arranged by size:
2301 1028 859 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2069 2211 2709 3471 3556 4627 2069
Size: 142 498 762 85 1071 2852
Fragments arranged by size:
2852 1071 762 498 142 85
BsmI GAATG_Cn'
Cuts at: 2921 3014 2921
Size: 93 5317
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 2069 2211 2670 2709 2729 3471
Size: 399 243 36 142 459 39 20 742
Cuts at: 3471 3556 4627 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 459 399 243 142 85
39 36 20
BspEI T'CCGG_A
Cuts at: 5073 5073
Size: 5410
BspHI T'CATG_A
Cuts at: 3299 5029 3299
Size: 1730 3680
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2166 2202 2653 2700 2765 844
Size: 1322 36 451 47 65 3489
Fragments arranged by size:
3489 1322 451 65 47 36
BsrBI CCG'CTC
Cuts at: 1394 1823 2257 3297 1394
Size: 429 434 1040 3507
Fragments arranged by size:
3507 1040 434 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3905 4079 65
Size: 1183 2657 174 1396
Fragments arranged by size:
2657 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4054 4241 1700
Size: 110 2244 187 2869
Fragments arranged by size:
2869 2244 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5410
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5410
BssSI C'ACGA_G
Cuts at: 3470 4482 3470
Size: 1012 4398
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2293 794
Size: 1499 3911
BstBI TT'CG_AA
Cuts at: 4214 4214
Size: 5410
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5410
BstXI CCAn_nnnn'nTGG
Cuts at: 2508 2508
Size: 5410
BstYI r'GATC_y
Cuts at: 1972 2740 3107 3492 3509 4961 5047 5059 5405
Size: 768 367 385 17 1452 86 12 346
Cuts at: 5405 1972
Size: 1977
Fragments arranged by size:
1977 1452 768 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2181 2507 2583 513
Size: 552 1116 326 76 3340
Fragments arranged by size:
3340 1116 552 326 76
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4671
Fragments arranged by size:
4671 683 56
BtsI GCAGTG_nn'
Cuts at: 2480 2926 3720 3740 2480
Size: 446 794 20 4150
Fragments arranged by size:
4150 794 446 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5410
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5375
DraI TTT'AAA
Cuts at: 2714 2714
Size: 5410
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2129 4417 809
Size: 507 813 2288 1802
Fragments arranged by size:
2288 1802 813 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2601 2857 3746
Size: 54 1425 50 275 18 771 256 889
Cuts at: 3746 5375 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 771 275 256 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2857 1537
Size: 275 1045 4090
Fragments arranged by size:
4090 1045 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3340 1353
Size: 1987 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2653 3997 4371 4517 387
Size: 1064 138 1064 1344 374 146 1280
Fragments arranged by size:
1344 1280 1064 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2067 2209 2067
Size: 142 5268
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2047 2318 2447 2486 2546 3473 4070
Size: 393 415 271 129 39 60 927 597
Cuts at: 4070 4857 5125 1239
Size: 787 268 1524
Fragments arranged by size:
1524 927 787 597 415 393 271 268
129 60 39
EcoNI CCTnn'n_nnAGG
Cuts at: 2396 2396
Size: 5410
EcoO109I rG'GnC_Cy
Cuts at: 2082 2218 2689 2082
Size: 136 471 4803
Fragments arranged by size:
4803 471 136
EcoRI G'AATT_C
Cuts at: 2719 3097 2719
Size: 378 5032
FspI TGC'GCA
Cuts at: 3916 3916
Size: 5410
HaeII r_GCGC'y
Cuts at: 1678 2021 2248 2426 4557 1678
Size: 343 227 178 2131 2531
Fragments arranged by size:
2531 2131 343 227 178
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4051 4083 4125 4157 1373
Size: 32 135 32 2479 32 42 32 2626
Fragments arranged by size:
2626 2479 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2754 3000 669
Size: 988 281 816 246 3079
Fragments arranged by size:
3079 988 816 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5410
HpaI GTT'AAC
Cuts at: 3000 3000
Size: 5410
KasI G'GCGC_C
Cuts at: 2017 2017
Size: 5410
KpnI G_GTAC'C
Cuts at: 2735 2735
Size: 5410
MfeI C'AATT_G
Cuts at: 3009 3009
Size: 5410
MluI A'CGCG_T
Cuts at: 5286 5286
Size: 5410
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2125 2145 2214 4524 4708 1305
Size: 820 20 69 2310 184 2007
Fragments arranged by size:
2310 2007 820 184 69 20
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3588
Fragments arranged by size:
3588 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2506 3368 3727 3886 518
Size: 648 843 497 862 359 159 2042
Fragments arranged by size:
2042 862 843 648 497 359 159
MspA1I CmG'CkG
Cuts at: 1606 2358 2416 2606 2826 3503 4651 4896 1606
Size: 752 58 190 220 677 1148 245 2120
Fragments arranged by size:
2120 1148 752 677 245 220 190 58
NarI GG'CG_CC
Cuts at: 2018 2018
Size: 5410
NcoI C'CATG_G
Cuts at: 513 1065 2507 513
Size: 552 1442 3416
Fragments arranged by size:
3416 1442 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5410
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5410
NotI GC'GGCC_GC
Cuts at: 2857 2857
Size: 5410
NspI r_CATG'y
Cuts at: 1132 1229 2768 4313 1132
Size: 97 1539 1545 2229
Fragments arranged by size:
2229 1545 1539 97
PasI CC'CwG_GG
Cuts at: 1348 2654 1348
Size: 1306 4104
PciI A'CATG_T
Cuts at: 4309 4309
Size: 5410
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5410
PmeI GTTT'AAAC
Cuts at: 2714 2714
Size: 5410
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3479 3511 4992 5024 3479
Size: 32 1481 32 3865
Fragments arranged by size:
3865 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2082 2689 2082
Size: 607 4803
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5410
PsiI TTA'TAA
Cuts at: 2980 2980
Size: 5410
PstI C_TGCA'G
Cuts at: 830 2199 2400 2475 2762 830
Size: 1369 201 75 287 3478
Fragments arranged by size:
3478 1369 287 201 75
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5410
PvuII CAG'CTG
Cuts at: 2358 2416 2358
Size: 58 5352
SacI G_AGCT'C
Cuts at: 2069 2211 2069
Size: 142 5268
SalI G'TCGA_C
Cuts at: 1936 2752 1936
Size: 816 4594
SanDI GG'GwC_CC
Cuts at: 2082 2082
Size: 5410
SbfI CC_TGCA'GG
Cuts at: 2199 2400 2762 2199
Size: 201 362 4847
Fragments arranged by size:
4847 362 201
ScaI AGT'ACT
Cuts at: 3658 5370 3658
Size: 1712 3698
SfcI C'TryA_G
Cuts at: 826 1045 2195 2396 2471 2758 3893 4574 4765
Size: 219 1150 201 75 287 1135 681 191
Cuts at: 4765 826
Size: 1471
Fragments arranged by size:
1471 1150 1135 681 287 219 201 191
75
SfoI GGC'GCC
Cuts at: 2019 2019
Size: 5410
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5410
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5410
SmaI CCC'GGG
Cuts at: 1872 2737 1872
Size: 865 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2261 3514 4415 4677 820
Size: 197 926 318 1253 901 262 1553
Fragments arranged by size:
1553 1253 926 901 318 262 197
SnaBI TAC'GTA
Cuts at: 493 3201 493
Size: 2708 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5410
SphI G_CATG'C
Cuts at: 1132 2768 1132
Size: 1636 3774
SspI AAT'ATT
Cuts at: 5 52 3334 5
Size: 47 3282 2081
Fragments arranged by size:
3282 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5410
StyI C'CwwG_G
Cuts at: 513 1065 2507 2849 513
Size: 552 1442 342 3074
Fragments arranged by size:
3074 1442 552 342
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3437 3454 3607 3792 4237 325
Size: 1255 1857 17 153 185 445 1498
Fragments arranged by size:
1857 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3656 5368 96
Size: 274 80 33 51 1443 1679 1712 138
Fragments arranged by size:
1712 1679 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2459 2491 5370 5402 2459
Size: 32 2879 32 2467
Fragments arranged by size:
2879 2467 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5410
XbaI T'CTAG_A
Cuts at: 2746 2746
Size: 5410
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5410
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5410
XmaI C'CCGG_G
Cuts at: 1870 2735 1870
Size: 865 4545
XmnI GAAnn'nnTTC
Cuts at: 3539 3539
Size: 5410
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3218 276
Size: 53 83 186 936 1684 2468
Fragments arranged by size:
2468 1684 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI
SfiI SrfI SwaI