Restriction map for
pFN21AE1724
(Circular) (Six-base) MAPSORT of: pFN21AE1724.seq Check: 9571 from: 1 to: 5407
pFN21AE1724 5407 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3217 278
Size: 53 83 186 936 1681 2468
Fragments arranged by size:
2468 1681 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2562 2750 1937
Size: 625 188 4594
Fragments arranged by size:
4594 625 188
Acc65I G'GTAC_C
Cuts at: 2728 2728
Size: 5407
AclI AA'CG_TT
Cuts at: 1563 3534 3907 1563
Size: 1971 373 3063
Fragments arranged by size:
3063 1971 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2498 3470 4854 5122 1632
Size: 866 972 1384 268 1917
Fragments arranged by size:
1917 1384 972 866 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5210
AflIII A'CryG_T
Cuts at: 1203 2557 4306 5283 1203
Size: 1354 1749 977 1327
Fragments arranged by size:
1749 1354 1327 977
AgeI A'CCGG_T
Cuts at: 4238 4238
Size: 5407
AhdI GACnn_n'nnGTC
Cuts at: 4136 4136
Size: 5407
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5407
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5407
AlwNI CAG_nnn'CTG
Cuts at: 2521 3150 4722 2521
Size: 629 1572 3206
Fragments arranged by size:
3206 1572 629
ApaLI G'TGCA_C
Cuts at: 3464 4620 3464
Size: 1156 4251
ApoI r'AATT_y
Cuts at: 1493 2716 2942 3094 5203 1493
Size: 1223 226 152 2109 1697
Fragments arranged by size:
2109 1697 1223 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3961 160
Size: 872 2929 1606
Fragments arranged by size:
2929 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5407
AvaI C'yCGr_G
Cuts at: 1870 1943 2732 1870
Size: 73 789 4545
Fragments arranged by size:
4545 789 73
BamHI G'GATC_C
Cuts at: 2737 3104 2737
Size: 367 5040
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2728 4183 618
Size: 325 217 627 941 1455 1842
Fragments arranged by size:
1842 1455 941 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2081 2284 2726 1391
Size: 690 203 442 4072
Fragments arranged by size:
4072 690 442 203
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2149 2262 928
Size: 971 250 113 4073
Fragments arranged by size:
4073 971 250 113
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3598 3632 1903
Size: 34 1661 34 3678
Fragments arranged by size:
3678 1661 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2279 2623 3299 4509 2279
Size: 344 676 1210 3177
Fragments arranged by size:
3177 1210 676 344
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5407
BfrBI ATG'CAT
Cuts at: 2065 2065
Size: 5407
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2141 4018 136
Size: 107 122 71 631 605 469 1877 1525
Fragments arranged by size:
1877 1525 631 605 469 122 107 71
BglII A'GATC_T
Cuts at: 5402 5402
Size: 5407
BlpI GC'TnA_GC
Cuts at: 2824 2824
Size: 5407
Bme1580I G_kGCm'C
Cuts at: 1790 3468 4624 1790
Size: 1678 1156 2573
Fragments arranged by size:
2573 1678 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5407
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3138 4096 442
Size: 416 53 83 399 24 1721 958 1753
Fragments arranged by size:
1753 1721 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5407
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2070 4067 1239
Size: 831 1997 2579
Fragments arranged by size:
2579 1997 831
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2687 3532 4397 4695 2687
Size: 845 865 298 3399
Fragments arranged by size:
3399 865 845 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4070 882
Size: 617 2571 2219
Fragments arranged by size:
2571 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2560 3198 493
Size: 711 1356 638 2702
Fragments arranged by size:
2702 1356 711 638
BsaBI GATnn'nnATC
Cuts at: 2391 5401 2391
Size: 3010 2397
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3214 3596 4231 275
Size: 53 83 186 936 1681 382 635 1451
Fragments arranged by size:
1681 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3840 4238 4512 4659 5070 3840
Size: 398 274 147 411 4177
Fragments arranged by size:
4177 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5182
Fragments arranged by size:
5182 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5407
BseYI C'CCAG_C
Cuts at: 4610 5106 4610
Size: 496 4911
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4834
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2857 3618 4646 1540
Size: 275 186 856 761 1028 2301
Fragments arranged by size:
2301 1028 856 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3468 3553 4624 3468
Size: 85 1071 4251
Fragments arranged by size:
4251 1071 85
BsmI GAATG_Cn'
Cuts at: 2361 2550 2918 3011 2361
Size: 189 368 93 4757
Fragments arranged by size:
4757 368 189 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2081 2284 2726 3468 3553 4624 1391
Size: 399 291 203 442 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 442 399 291 203 85
BspEI T'CCGG_A
Cuts at: 5070 5070
Size: 5407
BspHI T'CATG_A
Cuts at: 3296 5026 3296
Size: 1730 3677
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2590 2604 2762 844
Size: 1746 14 158 3489
Fragments arranged by size:
3489 1746 158 14
BsrBI CCG'CTC
Cuts at: 1394 1823 3294 1394
Size: 429 1471 3507
Fragments arranged by size:
3507 1471 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3902 4076 65
Size: 1183 2654 174 1396
Fragments arranged by size:
2654 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2071 4051 4238 1700
Size: 110 261 1980 187 2869
Fragments arranged by size:
2869 1980 261 187 110
BsrGI T'GTAC_A
Cuts at: 96 2449 96
Size: 2353 3054
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5407
BssSI C'ACGA_G
Cuts at: 2104 3467 4479 2104
Size: 1363 1012 3032
Fragments arranged by size:
3032 1363 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5407
BstBI TT'CG_AA
Cuts at: 4211 4211
Size: 5407
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5407
BstXI CCAn_nnnn'nTGG
Cuts at: 2095 2176 2411 2095
Size: 81 235 5091
Fragments arranged by size:
5091 235 81
BstYI r'GATC_y
Cuts at: 1972 2737 3104 3489 3506 4958 5044 5056 5402
Size: 765 367 385 17 1452 86 12 346
Cuts at: 5402 1972
Size: 1977
Fragments arranged by size:
1977 1452 765 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2563 2563
Size: 5407
BtgI C'CryG_G
Cuts at: 513 1065 2004 2082 2368 513
Size: 552 939 78 286 3552
Fragments arranged by size:
3552 939 552 286 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4668
Fragments arranged by size:
4668 683 56
BtsI GCAGTG_nn'
Cuts at: 2923 3717 3737 2923
Size: 794 20 4593
Fragments arranged by size:
4593 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5407
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5372
DraI TTT'AAA
Cuts at: 2711 2711
Size: 5407
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4414 809
Size: 507 3098 1802
Fragments arranged by size:
3098 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2085 2166 2854
Size: 54 1425 50 275 18 255 81 688
Cuts at: 2854 3743 5372 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 688 275 255 81 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2854 1537
Size: 275 1042 4090
Fragments arranged by size:
4090 1042 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3337 1353
Size: 1984 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3994 4368 4514 387
Size: 1064 138 2405 374 146 1280
Fragments arranged by size:
2405 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2070 2498 3470 4067 4854 5122 1239
Size: 393 438 428 972 597 787 268 1524
Fragments arranged by size:
1524 972 787 597 438 428 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2518 2518
Size: 5407
EcoRI G'AATT_C
Cuts at: 2716 3094 2716
Size: 378 5029
EcoRV GAT'ATC
Cuts at: 2381 2381
Size: 5407
FspI TGC'GCA
Cuts at: 3913 3913
Size: 5407
HaeII r_GCGC'y
Cuts at: 1678 4554 1678
Size: 2876 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4048 4080 4122 4154 1373
Size: 32 135 32 2476 32 42 32 2626
Fragments arranged by size:
2626 2476 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2605 2751 2997 669
Size: 988 281 667 146 246 3079
Fragments arranged by size:
3079 988 667 281 246 146
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5407
HpaI GTT'AAC
Cuts at: 2997 2997
Size: 5407
KpnI G_GTAC'C
Cuts at: 2732 2732
Size: 5407
MfeI C'AATT_G
Cuts at: 3006 3006
Size: 5407
MluI A'CGCG_T
Cuts at: 5283 5283
Size: 5407
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2463 4521 4705 1305
Size: 1158 2058 184 2007
Fragments arranged by size:
2058 2007 1158 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2087 2168 10
Size: 54 1425 343 255 81 3249
Fragments arranged by size:
3249 1425 343 255 81 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2060 2066 2174 3365 3724 3883 518
Size: 648 894 6 108 1191 359 159 2042
Fragments arranged by size:
2042 1191 894 648 359 159 108 6
MspA1I CmG'CkG
Cuts at: 1606 2823 3500 4648 4893 1606
Size: 1217 677 1148 245 2120
Fragments arranged by size:
2120 1217 1148 677 245
NaeI GCC'GGC
Cuts at: 2073 2073
Size: 5407
NcoI C'CATG_G
Cuts at: 513 1065 2004 2368 513
Size: 552 939 364 3552
Fragments arranged by size:
3552 939 552 364
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5407
NgoMIV G'CCGG_C
Cuts at: 2071 2071
Size: 5407
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5407
NotI GC'GGCC_GC
Cuts at: 2854 2854
Size: 5407
NsiI A_TGCA'T
Cuts at: 2067 2067
Size: 5407
NspI r_CATG'y
Cuts at: 1132 1229 2069 2765 4310 1132
Size: 97 840 696 1545 2229
Fragments arranged by size:
2229 1545 840 696 97
PasI CC'CwG_GG
Cuts at: 1348 2012 1348
Size: 664 4743
PciI A'CATG_T
Cuts at: 4306 4306
Size: 5407
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5407
PfoI T'CCnGG_A
Cuts at: 2047 2047
Size: 5407
PmeI GTTT'AAAC
Cuts at: 2711 2711
Size: 5407
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3476 3508 4989 5021 3476
Size: 32 1481 32 3862
Fragments arranged by size:
3862 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5407
PsiI TTA'TAA
Cuts at: 2977 2977
Size: 5407
PstI C_TGCA'G
Cuts at: 830 2022 2442 2601 2759 830
Size: 1192 420 159 158 3478
Fragments arranged by size:
3478 1192 420 159 158
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5407
SalI G'TCGA_C
Cuts at: 1936 2749 1936
Size: 813 4594
SbfI CC_TGCA'GG
Cuts at: 2601 2759 2601
Size: 158 5249
ScaI AGT'ACT
Cuts at: 3655 5367 3655
Size: 1712 3695
SfcI C'TryA_G
Cuts at: 826 1045 2018 2438 2597 2755 3890 4571 4762
Size: 219 973 420 159 158 1135 681 191
Cuts at: 4762 826
Size: 1471
Fragments arranged by size:
1471 1135 973 681 420 219 191 159
158
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5407
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5407
SmaI CCC'GGG
Cuts at: 1872 2734 1872
Size: 862 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2666 3511 4412 4674 820
Size: 197 926 723 845 901 262 1553
Fragments arranged by size:
1553 926 901 845 723 262 197
SnaBI TAC'GTA
Cuts at: 493 3198 493
Size: 2705 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5407
SphI G_CATG'C
Cuts at: 1132 2765 1132
Size: 1633 3774
SspI AAT'ATT
Cuts at: 5 52 3331 5
Size: 47 3279 2081
Fragments arranged by size:
3279 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5407
StyI C'CwwG_G
Cuts at: 513 1065 2004 2368 2846 513
Size: 552 939 364 478 3074
Fragments arranged by size:
3074 939 552 478 364
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3434 3451 3604 3789 4234 325
Size: 1255 1854 17 153 185 445 1498
Fragments arranged by size:
1854 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2418 2449 2640
Size: 274 80 33 51 1443 441 31 191
Cuts at: 2640 3653 5365 96
Size: 1013 1712 138
Fragments arranged by size:
1712 1443 1013 441 274 191 138 80
51 33 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5367 5399 5367
Size: 32 5375
Tth111I GACn'n_nGTC
Cuts at: 1316 2225 1316
Size: 909 4498
XbaI T'CTAG_A
Cuts at: 2743 2743
Size: 5407
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2137 2137
Size: 5407
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5407
XmaI C'CCGG_G
Cuts at: 1870 2732 1870
Size: 862 4545
XmnI GAAnn'nnTTC
Cuts at: 3536 3536
Size: 5407
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3215 276
Size: 53 83 186 936 1681 2468
Fragments arranged by size:
2468 1681 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI
EcoNI FalI FseI FspAI KasI NarI NruI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI