Restriction map for
pFN21AE1723
(Circular) (Six-base) MAPSORT of: pFN21AE1723.seq Check: 2275 from: 1 to: 5407
pFN21AE1723 5407 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3217 278
Size: 53 83 186 936 1681 2468
Fragments arranged by size:
2468 1681 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2750 1937
Size: 813 4594
Acc65I G'GTAC_C
Cuts at: 2728 2728
Size: 5407
AclI AA'CG_TT
Cuts at: 1563 2706 3534 3907 1563
Size: 1143 828 373 3063
Fragments arranged by size:
3063 1143 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3470 4854 5122 1632
Size: 1838 1384 268 1917
Fragments arranged by size:
1917 1838 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5210
AflIII A'CryG_T
Cuts at: 1203 2119 4306 5283 1203
Size: 916 2187 977 1327
Fragments arranged by size:
2187 1327 977 916
AgeI A'CCGG_T
Cuts at: 4238 4238
Size: 5407
AhdI GACnn_n'nnGTC
Cuts at: 4136 4136
Size: 5407
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5407
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2036 1184
Size: 852 4555
AlwNI CAG_nnn'CTG
Cuts at: 2199 2686 3150 4722 2199
Size: 487 464 1572 2884
Fragments arranged by size:
2884 1572 487 464
ApaLI G'TGCA_C
Cuts at: 3464 4620 3464
Size: 1156 4251
ApoI r'AATT_y
Cuts at: 1493 2716 2942 3094 5203 1493
Size: 1223 226 152 2109 1697
Fragments arranged by size:
2109 1697 1223 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3961 160
Size: 872 2929 1606
Fragments arranged by size:
2929 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5407
AvaI C'yCGr_G
Cuts at: 1870 1943 2055 2078 2732 1870
Size: 73 112 23 654 4545
Fragments arranged by size:
4545 654 112 73 23
BamHI G'GATC_C
Cuts at: 2737 3104 2737
Size: 367 5040
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2422 2728 4183 618
Size: 325 217 627 635 306 1455 1842
Fragments arranged by size:
1842 1455 635 627 325 306 217
BanII G_rGCy'C
Cuts at: 1391 2726 1391
Size: 1335 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2360 928
Size: 971 461 3975
Fragments arranged by size:
3975 971 461
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3598 3632 1903
Size: 34 1661 34 3678
Fragments arranged by size:
3678 1661 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3299 4509 3299
Size: 1210 4197
BclI T'GATC_A
Cuts at: 1548 2160 1548
Size: 612 4795
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2264 2599 4018
Size: 107 122 71 631 605 592 335 1419
Cuts at: 4018 136
Size: 1525
Fragments arranged by size:
1525 1419 631 605 592 335 122 107
71
BglII A'GATC_T
Cuts at: 5402 5402
Size: 5407
BlpI GC'TnA_GC
Cuts at: 2824 2824
Size: 5407
Bme1580I G_kGCm'C
Cuts at: 1790 3468 4624 1790
Size: 1678 1156 2573
Fragments arranged by size:
2573 1678 1156
BmgBI CAC'GTC
Cuts at: 1318 2254 1318
Size: 936 4471
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2618 3138 4096
Size: 416 53 83 399 24 1201 520 958
Cuts at: 4096 442
Size: 1753
Fragments arranged by size:
1753 1201 958 520 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5407
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4067 1239
Size: 2828 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3532 4397 4695 3532
Size: 865 298 4244
Fragments arranged by size:
4244 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4070 882
Size: 617 2571 2219
Fragments arranged by size:
2571 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3198 493
Size: 711 1994 2702
Fragments arranged by size:
2702 1994 711
BsaBI GATnn'nnATC
Cuts at: 5401 5401
Size: 5407
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3214 3596 4231 275
Size: 53 83 186 936 1681 382 635 1451
Fragments arranged by size:
1681 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2211 3840 4238 4512 4659 5070 2211
Size: 1629 398 274 147 411 2548
Fragments arranged by size:
2548 1629 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5182
Fragments arranged by size:
5182 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5407
BseYI C'CCAG_C
Cuts at: 2045 4610 5106 2045
Size: 2565 496 2346
Fragments arranged by size:
2565 2346 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2185 2355 1163
Size: 573 449 170 4215
Fragments arranged by size:
4215 573 449 170
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2857 3618 4646 1540
Size: 275 186 856 761 1028 2301
Fragments arranged by size:
2301 1028 856 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3468 3553 4624 3468
Size: 85 1071 4251
Fragments arranged by size:
4251 1071 85
BsmI GAATG_Cn'
Cuts at: 2918 3011 2918
Size: 93 5314
BsmBI CGTCTCn'nnnn_
Cuts at: 2110 2244 2110
Size: 134 5273
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2726 3468 3553 4624 1391
Size: 399 936 742 85 1071 2174
Fragments arranged by size:
2174 1071 936 742 399 85
BspEI T'CCGG_A
Cuts at: 5070 5070
Size: 5407
BspHI T'CATG_A
Cuts at: 3296 5026 3296
Size: 1730 3677
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2028 2762 844
Size: 1184 734 3489
Fragments arranged by size:
3489 1184 734
BsrBI CCG'CTC
Cuts at: 1394 1823 3294 1394
Size: 429 1471 3507
Fragments arranged by size:
3507 1471 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2696 3902 4076 65
Size: 1183 1448 1206 174 1396
Fragments arranged by size:
1448 1396 1206 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4051 4238 1700
Size: 110 2241 187 2869
Fragments arranged by size:
2869 2241 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5407
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5407
BssSI C'ACGA_G
Cuts at: 3467 4479 3467
Size: 1012 4395
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2199 2469 2686 794
Size: 1405 270 217 3515
Fragments arranged by size:
3515 1405 270 217
BstBI TT'CG_AA
Cuts at: 4211 4211
Size: 5407
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5407
BstYI r'GATC_y
Cuts at: 1972 2064 2737 3104 3489 3506 4958 5044 5056
Size: 92 673 367 385 17 1452 86 12
Cuts at: 5056 5402 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 673 385 367 346 92 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2584 2584
Size: 5407
BtgI C'CryG_G
Cuts at: 513 1065 2220 2337 2649 513
Size: 552 1155 117 312 3271
Fragments arranged by size:
3271 1155 552 312 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4668
Fragments arranged by size:
4668 683 56
BtsI GCAGTG_nn'
Cuts at: 2923 3717 3737 2923
Size: 794 20 4593
Fragments arranged by size:
4593 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5407
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5372
DraI TTT'AAA
Cuts at: 2711 2711
Size: 5407
DraIII CAC_nnn'GTG
Cuts at: 2554 2554
Size: 5407
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2124 4414 809
Size: 507 808 2290 1802
Fragments arranged by size:
2290 1802 808 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2340 2430 2854
Size: 54 1425 50 275 18 510 90 424
Cuts at: 2854 3743 5372 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 510 424 275 90 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2854 1537
Size: 275 1042 4090
Fragments arranged by size:
4090 1042 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3337 1353
Size: 1984 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3994 4368 4514 387
Size: 1064 138 2405 374 146 1280
Fragments arranged by size:
2405 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3470 4067 4854 5122 1239
Size: 393 1838 597 787 268 1524
Fragments arranged by size:
1838 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2389 2389
Size: 5407
EcoO109I rG'GnC_Cy
Cuts at: 2266 2382 2266
Size: 116 5291
EcoRI G'AATT_C
Cuts at: 2716 3094 2716
Size: 378 5029
FspI TGC'GCA
Cuts at: 3913 3913
Size: 5407
HaeII r_GCGC'y
Cuts at: 1678 2231 4554 1678
Size: 553 2323 2531
Fragments arranged by size:
2531 2323 553
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4048 4080 4122 4154 1373
Size: 32 135 32 2476 32 42 32 2626
Fragments arranged by size:
2626 2476 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2751 2997 669
Size: 988 281 813 246 3079
Fragments arranged by size:
3079 988 813 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5407
HpaI GTT'AAC
Cuts at: 2997 2997
Size: 5407
KpnI G_GTAC'C
Cuts at: 2732 2732
Size: 5407
MfeI C'AATT_G
Cuts at: 3006 3006
Size: 5407
MluI A'CGCG_T
Cuts at: 2119 5283 2119
Size: 3164 2243
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4521 4705 1305
Size: 3216 184 2007
Fragments arranged by size:
3216 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2342 10
Size: 54 1425 343 510 3075
Fragments arranged by size:
3075 1425 510 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3365 3724 3883 518
Size: 648 2199 359 159 2042
Fragments arranged by size:
2199 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2428 2823 3500 4648 4893 1606
Size: 822 395 677 1148 245 2120
Fragments arranged by size:
2120 1148 822 677 395 245
NcoI C'CATG_G
Cuts at: 513 1065 2220 2337 513
Size: 552 1155 117 3583
Fragments arranged by size:
3583 1155 552 117
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5407
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5407
NotI GC'GGCC_GC
Cuts at: 2854 2854
Size: 5407
NspI r_CATG'y
Cuts at: 1132 1229 2765 4310 1132
Size: 97 1536 1545 2229
Fragments arranged by size:
2229 1545 1536 97
PasI CC'CwG_GG
Cuts at: 1348 2269 1348
Size: 921 4486
PciI A'CATG_T
Cuts at: 4306 4306
Size: 5407
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5407
PfoI T'CCnGG_A
Cuts at: 2475 2475
Size: 5407
PmeI GTTT'AAAC
Cuts at: 2711 2711
Size: 5407
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3476 3508 4989 5021 3476
Size: 32 1481 32 3862
Fragments arranged by size:
3862 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2382 2382
Size: 5407
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5407
PsiI TTA'TAA
Cuts at: 2977 2977
Size: 5407
PstI C_TGCA'G
Cuts at: 830 2759 830
Size: 1929 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5407
SalI G'TCGA_C
Cuts at: 1936 2749 1936
Size: 813 4594
SbfI CC_TGCA'GG
Cuts at: 2759 2759
Size: 5407
ScaI AGT'ACT
Cuts at: 3655 5367 3655
Size: 1712 3695
SexAI A'CCwGG_T
Cuts at: 2418 2418
Size: 5407
SfcI C'TryA_G
Cuts at: 826 1045 2185 2755 3890 4571 4762 826
Size: 219 1140 570 1135 681 191 1471
Fragments arranged by size:
1471 1140 1135 681 570 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2264 2264
Size: 5407
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5407
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5407
SmaI CCC'GGG
Cuts at: 1872 2057 2734 1872
Size: 185 677 4545
Fragments arranged by size:
4545 677 185
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3511 4412 4674 820
Size: 197 926 1568 901 262 1553
Fragments arranged by size:
1568 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3198 493
Size: 2705 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5407
SphI G_CATG'C
Cuts at: 1132 2765 1132
Size: 1633 3774
SspI AAT'ATT
Cuts at: 5 52 3331 5
Size: 47 3279 2081
Fragments arranged by size:
3279 2081 47
StuI AGG'CCT
Cuts at: 1517 2582 1517
Size: 1065 4342
StyI C'CwwG_G
Cuts at: 513 1065 2220 2337 2846 513
Size: 552 1155 117 509 3074
Fragments arranged by size:
3074 1155 552 509 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3434 3451 3604 3789 4234 325
Size: 1255 1854 17 153 185 445 1498
Fragments arranged by size:
1854 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2145 3653 5365
Size: 274 80 33 51 1443 168 1508 1712
Cuts at: 5365 96
Size: 138
Fragments arranged by size:
1712 1508 1443 274 168 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5367 5399 5367
Size: 32 5375
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5407
XbaI T'CTAG_A
Cuts at: 2743 2743
Size: 5407
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2416 2416
Size: 5407
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5407
XmaI C'CCGG_G
Cuts at: 1870 2055 2732 1870
Size: 185 677 4545
Fragments arranged by size:
4545 677 185
XmnI GAAnn'nnTTC
Cuts at: 3536 3536
Size: 5407
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3215 276
Size: 53 83 186 936 1681 2468
Fragments arranged by size:
2468 1681 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SfoI SrfI SwaI