Restriction map for
pFN21AE1722
(Circular) (Six-base) MAPSORT of: pFN21AE1722.seq Check: 8932 from: 1 to: 5404
pFN21AE1722 5404 bp
With 182 enzymes: SgfI *
June 19, 2019 18:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2687 2687
Size: 5404
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3214 278
Size: 53 83 186 936 1678 2468
Fragments arranged by size:
2468 1678 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2747 1937
Size: 810 4594
Acc65I G'GTAC_C
Cuts at: 2725 2725
Size: 5404
AclI AA'CG_TT
Cuts at: 1563 3531 3904 1563
Size: 1968 373 3063
Fragments arranged by size:
3063 1968 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3467 4851 5119 1632
Size: 1835 1384 268 1917
Fragments arranged by size:
1917 1835 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5207
AflIII A'CryG_T
Cuts at: 1203 4303 5280 1203
Size: 3100 977 1327
Fragments arranged by size:
3100 1327 977
AgeI A'CCGG_T
Cuts at: 4235 4235
Size: 5404
AhdI GACnn_n'nnGTC
Cuts at: 2380 4133 2380
Size: 1753 3651
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5404
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2135 1184
Size: 951 4453
AlwNI CAG_nnn'CTG
Cuts at: 2332 3147 4719 2332
Size: 815 1572 3017
Fragments arranged by size:
3017 1572 815
ApaI G_GGCC'C
Cuts at: 2317 2423 2530 2317
Size: 106 107 5191
Fragments arranged by size:
5191 107 106
ApaLI G'TGCA_C
Cuts at: 3461 4617 3461
Size: 1156 4248
ApoI r'AATT_y
Cuts at: 1493 2713 2939 3091 5200 1493
Size: 1220 226 152 2109 1697
Fragments arranged by size:
2109 1697 1220 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3958 160
Size: 872 2926 1606
Fragments arranged by size:
2926 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5404
AvaI C'yCGr_G
Cuts at: 1870 1943 2729 1870
Size: 73 786 4545
Fragments arranged by size:
4545 786 73
BamHI G'GATC_C
Cuts at: 2734 3101 2734
Size: 367 5037
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2027 2147 2664 2725 4180
Size: 325 217 627 240 120 517 61 1455
Cuts at: 4180 618
Size: 1842
Fragments arranged by size:
1842 1455 627 517 325 240 217 120
61
BanII G_rGCy'C
Cuts at: 1391 2060 2089 2317 2423 2530 2634 2723 1391
Size: 669 29 228 106 107 104 89 4072
Fragments arranged by size:
4072 669 228 107 106 104 89 29
BbeI G_GCGC'C
Cuts at: 2151 2151
Size: 5404
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2353 2663 928
Size: 971 454 310 3669
Fragments arranged by size:
3669 971 454 310
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3595 3629 1903
Size: 34 1658 34 3678
Fragments arranged by size:
3678 1658 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3296 4506 3296
Size: 1210 4194
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5404
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2688 4015 136
Size: 107 122 71 631 605 1016 1327 1525
Fragments arranged by size:
1525 1327 1016 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5399 5399
Size: 5404
BlpI GC'TnA_GC
Cuts at: 2821 2821
Size: 5404
Bme1580I G_kGCm'C
Cuts at: 1790 2317 2423 2530 3465 4621 1790
Size: 527 106 107 935 1156 2573
Fragments arranged by size:
2573 1156 935 527 107 106
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5404
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3135 4093 442
Size: 416 53 83 399 24 1718 958 1753
Fragments arranged by size:
1753 1718 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5404
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2502 4064 1239
Size: 1263 1562 2579
Fragments arranged by size:
2579 1562 1263
Bpu10I CC'TnA_GC
Cuts at: 2088 2088
Size: 5404
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3529 4394 4692 3529
Size: 865 298 4241
Fragments arranged by size:
4241 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2534 4067 882
Size: 617 1035 1533 2219
Fragments arranged by size:
2219 1533 1035 617
BsaAI yAC'GTr
Cuts at: 493 1204 3195 493
Size: 711 1991 2702
Fragments arranged by size:
2702 1991 711
BsaBI GATnn'nnATC
Cuts at: 5398 5398
Size: 5404
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2148 2358 3211 3593
Size: 53 83 186 936 615 210 853 382
Cuts at: 3593 4228 275
Size: 635 1451
Fragments arranged by size:
1451 936 853 635 615 382 210 186
83 53
BsaWI w'CCGG_w
Cuts at: 3837 4235 4509 4656 5067 3837
Size: 398 274 147 411 4174
Fragments arranged by size:
4174 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5179
Fragments arranged by size:
5179 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2585 1186
Size: 1399 4005
BseYI C'CCAG_C
Cuts at: 2353 2433 2520 4607 5103 2353
Size: 80 87 2087 496 2654
Fragments arranged by size:
2654 2087 496 87 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4831
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2854 3615 4643 1540
Size: 275 186 853 761 1028 2301
Fragments arranged by size:
2301 1028 853 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3465 3550 4621 3465
Size: 85 1071 4248
Fragments arranged by size:
4248 1071 85
BsmI GAATG_Cn'
Cuts at: 2271 2915 3008 2271
Size: 644 93 4667
Fragments arranged by size:
4667 644 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2388 2388
Size: 5404
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2060 2089 2317 2423 2530 2634 2723
Size: 399 270 29 228 106 107 104 89
Cuts at: 2723 3465 3550 4621 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 270 228 107 106
104 89 85 29
BspEI T'CCGG_A
Cuts at: 5067 5067
Size: 5404
BspHI T'CATG_A
Cuts at: 3293 5023 3293
Size: 1730 3674
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2687 2759 844
Size: 1843 72 3489
Fragments arranged by size:
3489 1843 72
BsrBI CCG'CTC
Cuts at: 1394 1823 3291 1394
Size: 429 1468 3507
Fragments arranged by size:
3507 1468 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3899 4073 65
Size: 1183 2651 174 1396
Fragments arranged by size:
2651 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4048 4235 1700
Size: 110 2238 187 2869
Fragments arranged by size:
2869 2238 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5404
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5404
BssSI C'ACGA_G
Cuts at: 2447 3464 4476 2447
Size: 1017 1012 3375
Fragments arranged by size:
3375 1017 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5404
BstBI TT'CG_AA
Cuts at: 4208 4208
Size: 5404
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5404
BstXI CCAn_nnnn'nTGG
Cuts at: 2158 2158
Size: 5404
BstYI r'GATC_y
Cuts at: 1972 2598 2734 3101 3486 3503 4955 5041 5053
Size: 626 136 367 385 17 1452 86 12
Cuts at: 5053 5399 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 626 385 367 346 136 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2619 2634 513
Size: 552 939 615 15 3283
Fragments arranged by size:
3283 939 615 552 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4665
Fragments arranged by size:
4665 683 56
BtsI GCAGTG_nn'
Cuts at: 2475 2920 3714 3734 2475
Size: 445 794 20 4145
Fragments arranged by size:
4145 794 445 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5404
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5369
DraI TTT'AAA
Cuts at: 2708 2708
Size: 5404
DraIII CAC_nnn'GTG
Cuts at: 2536 2536
Size: 5404
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4411 809
Size: 507 3095 1802
Fragments arranged by size:
3095 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2547 2700
Size: 54 1425 50 275 18 216 501 153
Cuts at: 2700 2851 3740 5369 8
Size: 151 889 1629 43
Fragments arranged by size:
1629 1425 889 501 275 216 153 151
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2851 1537
Size: 275 1039 4090
Fragments arranged by size:
4090 1039 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3334 1353
Size: 1981 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3991 4365 4511 387
Size: 1064 138 2402 374 146 1280
Fragments arranged by size:
2402 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2502 3467 4064 4851 5119 1239
Size: 393 870 965 597 787 268 1524
Fragments arranged by size:
1524 965 870 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2313 2314 2419 2420 2526 2643 2313
Size: 1 105 1 106 117 5074
Fragments arranged by size:
5074 117 106 105 1 1
EcoRI G'AATT_C
Cuts at: 2713 3091 2713
Size: 378 5026
FspI TGC'GCA
Cuts at: 3910 3910
Size: 5404
HaeII r_GCGC'y
Cuts at: 1678 2151 4551 1678
Size: 473 2400 2531
Fragments arranged by size:
2531 2400 473
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2363 2366 2395 2398 4045
Size: 32 135 32 791 3 29 3 1647
Cuts at: 4045 4077 4119 4151 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1647 791 135 42 32 32 32
32 29 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2250 2748 2994 669
Size: 988 281 312 498 246 3079
Fragments arranged by size:
3079 988 498 312 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5404
HpaI GTT'AAC
Cuts at: 2994 2994
Size: 5404
KasI G'GCGC_C
Cuts at: 2147 2147
Size: 5404
KpnI G_GTAC'C
Cuts at: 2729 2729
Size: 5404
MfeI C'AATT_G
Cuts at: 3003 3003
Size: 5404
MluI A'CGCG_T
Cuts at: 5280 5280
Size: 5404
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4518 4702 1305
Size: 3213 184 2007
Fragments arranged by size:
3213 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2048 10
Size: 54 1425 343 216 3366
Fragments arranged by size:
3366 1425 343 216 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3362 3721 3880 518
Size: 648 2196 359 159 2042
Fragments arranged by size:
2196 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2119 2185 2395 2498 2520 2820 3497 4645
Size: 513 66 210 103 22 300 677 1148
Cuts at: 4645 4890 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 513 300 245 210 103
66 22
NarI GG'CG_CC
Cuts at: 2148 2148
Size: 5404
NcoI C'CATG_G
Cuts at: 513 1065 2004 2619 2634 513
Size: 552 939 615 15 3283
Fragments arranged by size:
3283 939 615 552 15
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5404
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5404
NotI GC'GGCC_GC
Cuts at: 2851 2851
Size: 5404
NspI r_CATG'y
Cuts at: 1132 1229 2762 4307 1132
Size: 97 1533 1545 2229
Fragments arranged by size:
2229 1545 1533 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5404
PciI A'CATG_T
Cuts at: 4303 4303
Size: 5404
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2332 2536 1108
Size: 1224 204 3976
Fragments arranged by size:
3976 1224 204
PfoI T'CCnGG_A
Cuts at: 2226 2610 2226
Size: 384 5020
PmeI GTTT'AAAC
Cuts at: 2708 2708
Size: 5404
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3473 3505 4986 5018 3473
Size: 32 1481 32 3859
Fragments arranged by size:
3859 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2643 2643
Size: 5404
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5404
PsiI TTA'TAA
Cuts at: 2974 2974
Size: 5404
PspOMI G'GGCC_C
Cuts at: 2313 2419 2526 2313
Size: 106 107 5191
Fragments arranged by size:
5191 107 106
PstI C_TGCA'G
Cuts at: 830 2400 2506 2756 830
Size: 1570 106 250 3478
Fragments arranged by size:
3478 1570 250 106
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5404
PvuII CAG'CTG
Cuts at: 2119 2185 2395 2520 2119
Size: 66 210 125 5003
Fragments arranged by size:
5003 210 125 66
SalI G'TCGA_C
Cuts at: 1936 2746 1936
Size: 810 4594
SbfI CC_TGCA'GG
Cuts at: 2756 2756
Size: 5404
ScaI AGT'ACT
Cuts at: 3652 5364 3652
Size: 1712 3692
SfcI C'TryA_G
Cuts at: 826 1045 2396 2502 2647 2752 3887 4568 4759
Size: 219 1351 106 145 105 1135 681 191
Cuts at: 4759 826
Size: 1471
Fragments arranged by size:
1471 1351 1135 681 219 191 145 106
105
SfoI GGC'GCC
Cuts at: 2149 2149
Size: 5404
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5404
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5404
SmaI CCC'GGG
Cuts at: 1872 2731 1872
Size: 859 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3508 4409 4671 820
Size: 197 926 1565 901 262 1553
Fragments arranged by size:
1565 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3195 493
Size: 2702 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5404
SphI G_CATG'C
Cuts at: 1132 2762 1132
Size: 1630 3774
SspI AAT'ATT
Cuts at: 5 52 3328 5
Size: 47 3276 2081
Fragments arranged by size:
3276 2081 47
StuI AGG'CCT
Cuts at: 1517 2576 1517
Size: 1059 4345
StyI C'CwwG_G
Cuts at: 513 1065 2004 2619 2634 2843 513
Size: 552 939 615 15 209 3074
Fragments arranged by size:
3074 939 615 552 209 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3431 3448 3601 3786 4231 325
Size: 1255 1851 17 153 185 445 1498
Fragments arranged by size:
1851 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2127 3650 5362
Size: 274 80 33 51 1443 150 1523 1712
Cuts at: 5362 96
Size: 138
Fragments arranged by size:
1712 1523 1443 274 150 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5364 5396 5364
Size: 32 5372
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5404
XbaI T'CTAG_A
Cuts at: 2740 2740
Size: 5404
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5404
XmaI C'CCGG_G
Cuts at: 1870 2729 1870
Size: 859 4545
XmnI GAAnn'nnTTC
Cuts at: 3533 3533
Size: 5404
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3212 276
Size: 53 83 186 936 1678 2468
Fragments arranged by size:
2468 1678 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI XcmI