Restriction map for
pFN21AE1719
(Circular) (Six-base) MAPSORT of: pFN21AE1719.seq Check: 8125 from: 1 to: 5380
pFN21AE1719 5380 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3190 278
Size: 53 83 186 936 1654 2468
Fragments arranged by size:
2468 1654 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2723 1937
Size: 786 4594
Acc65I G'GTAC_C
Cuts at: 2517 2701 2517
Size: 184 5196
AclI AA'CG_TT
Cuts at: 1563 3507 3880 1563
Size: 1944 373 3063
Fragments arranged by size:
3063 1944 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3443 4827 5095 1632
Size: 1811 1384 268 1917
Fragments arranged by size:
1917 1811 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5183
AflIII A'CryG_T
Cuts at: 1203 4279 5256 1203
Size: 3076 977 1327
Fragments arranged by size:
3076 1327 977
AgeI A'CCGG_T
Cuts at: 4211 4211
Size: 5380
AhdI GACnn_n'nnGTC
Cuts at: 4109 4109
Size: 5380
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5380
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5380
AlwNI CAG_nnn'CTG
Cuts at: 3123 4695 3123
Size: 1572 3808
ApaLI G'TGCA_C
Cuts at: 3437 4593 3437
Size: 1156 4224
ApoI r'AATT_y
Cuts at: 1493 2689 2915 3067 5176 1493
Size: 1196 226 152 2109 1697
Fragments arranged by size:
2109 1697 1196 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3934 160
Size: 872 2902 1606
Fragments arranged by size:
2902 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5380
AvaI C'yCGr_G
Cuts at: 1870 1943 2057 2648 2705 1870
Size: 73 114 591 57 4545
Fragments arranged by size:
4545 591 114 73 57
BamHI G'GATC_C
Cuts at: 2710 3077 2710
Size: 367 5013
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2105 2282 2517 2701 4156
Size: 325 217 627 318 177 235 184 1455
Cuts at: 4156 618
Size: 1842
Fragments arranged by size:
1842 1455 627 325 318 235 217 184
177
BanII G_rGCy'C
Cuts at: 1391 2058 2699 1391
Size: 667 641 4072
Fragments arranged by size:
4072 667 641
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2576 928
Size: 971 677 3732
Fragments arranged by size:
3732 971 677
BbvCI CC'TCA_GC
Cuts at: 2202 2391 2202
Size: 189 5191
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3571 3605 1903
Size: 34 1634 34 3678
Fragments arranged by size:
3678 1634 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2287 3272 4482 2287
Size: 985 1210 3185
Fragments arranged by size:
3185 1210 985
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5380
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2179 3991
Size: 107 122 71 631 605 337 170 1812
Cuts at: 3991 136
Size: 1525
Fragments arranged by size:
1812 1525 631 605 337 170 122 107
71
BglII A'GATC_T
Cuts at: 5375 5375
Size: 5380
BlpI GC'TnA_GC
Cuts at: 2797 2797
Size: 5380
Bme1580I G_kGCm'C
Cuts at: 1790 2285 3441 4597 1790
Size: 495 1156 1156 2573
Fragments arranged by size:
2573 1156 1156 495
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5380
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3111 4069 442
Size: 416 53 83 399 24 1694 958 1753
Fragments arranged by size:
1753 1694 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5380
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2237 2627 4040 1239
Size: 998 390 1413 2579
Fragments arranged by size:
2579 1413 998 390
Bpu10I CC'TnA_GC
Cuts at: 2202 2391 2202
Size: 189 5191
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3505 4370 4668 3505
Size: 865 298 4217
Fragments arranged by size:
4217 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4043 882
Size: 617 2544 2219
Fragments arranged by size:
2544 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3171 493
Size: 711 1967 2702
Fragments arranged by size:
2702 1967 711
BsaBI GATnn'nnATC
Cuts at: 5374 5374
Size: 5380
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3187 3569 4204 275
Size: 53 83 186 936 1654 382 635 1451
Fragments arranged by size:
1654 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3813 4211 4485 4632 5043 3813
Size: 398 274 147 411 4150
Fragments arranged by size:
4150 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5155
Fragments arranged by size:
5155 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2123 1186
Size: 937 4443
BseYI C'CCAG_C
Cuts at: 2365 4583 5079 2365
Size: 2218 496 2666
Fragments arranged by size:
2666 2218 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4807
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2072 2183 2830 3591 4619 1540
Size: 275 186 71 111 647 761 1028 2301
Fragments arranged by size:
2301 1028 761 647 275 186 111 71
BsiHKAI G_wGCw'C
Cuts at: 3441 3526 4597 3441
Size: 85 1071 4224
Fragments arranged by size:
4224 1071 85
BsmI GAATG_Cn'
Cuts at: 2392 2891 2984 2392
Size: 499 93 4788
Fragments arranged by size:
4788 499 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 2285 2699 3441 3526 4597 1391
Size: 399 268 227 414 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 414 399 268 227 85
BspEI T'CCGG_A
Cuts at: 5043 5043
Size: 5380
BspHI T'CATG_A
Cuts at: 2547 3269 4999 2547
Size: 722 1730 2928
Fragments arranged by size:
2928 1730 722
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2735 844
Size: 1891 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3267 1394
Size: 429 1444 3507
Fragments arranged by size:
3507 1444 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3875 4049 65
Size: 1183 2627 174 1396
Fragments arranged by size:
2627 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2067 2071 4024 4211 1700
Size: 110 257 4 1953 187 2869
Fragments arranged by size:
2869 1953 257 187 110 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5380
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5380
BssSI C'ACGA_G
Cuts at: 3440 4452 3440
Size: 1012 4368
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5380
BstBI TT'CG_AA
Cuts at: 4184 4184
Size: 5380
BstEII G'GTnAC_C
Cuts at: 1184 2344 1184
Size: 1160 4220
BstXI CCAn_nnnn'nTGG
Cuts at: 2605 2605
Size: 5380
BstYI r'GATC_y
Cuts at: 1972 2710 3077 3462 3479 4931 5017 5029 5375
Size: 738 367 385 17 1452 86 12 346
Cuts at: 5375 1972
Size: 1977
Fragments arranged by size:
1977 1452 738 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2101 2188 513
Size: 552 939 97 87 3705
Fragments arranged by size:
3705 939 552 97 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4641
Fragments arranged by size:
4641 683 56
BtsI GCAGTG_nn'
Cuts at: 2896 3690 3710 2896
Size: 794 20 4566
Fragments arranged by size:
4566 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5380
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5345
DraI TTT'AAA
Cuts at: 2684 2684
Size: 5380
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4387 809
Size: 507 3071 1802
Fragments arranged by size:
3071 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 2069 2180
Size: 54 1425 50 275 18 186 53 111
Cuts at: 2180 2379 2506 2613 2827 3716 5345 8
Size: 199 127 107 214 889 1629 43
Fragments arranged by size:
1629 1425 889 275 214 199 186 127
111 107 54 53 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2069 2180 2827 1537
Size: 275 257 111 647 4090
Fragments arranged by size:
4090 647 275 257 111
EarI CTCTTCn'nnn_
Cuts at: 1353 3310 1353
Size: 1957 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3967 4341 4487 387
Size: 1064 138 2378 374 146 1280
Fragments arranged by size:
2378 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2237 2627 3443 4040 4827 5095 1239
Size: 393 605 390 816 597 787 268 1524
Fragments arranged by size:
1524 816 787 605 597 393 390 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2531 2531
Size: 5380
EcoRI G'AATT_C
Cuts at: 2689 3067 2689
Size: 378 5002
FspI TGC'GCA
Cuts at: 3886 3886
Size: 5380
HaeII r_GCGC'y
Cuts at: 1678 2015 2361 4527 1678
Size: 337 346 2166 2531
Fragments arranged by size:
2531 2166 346 337
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2285 2317 4021 4053 4095
Size: 32 135 32 713 32 1704 32 42
Cuts at: 4095 4127 1373
Size: 32 2626
Fragments arranged by size:
2626 1704 713 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2724 2970 669
Size: 988 281 786 246 3079
Fragments arranged by size:
3079 988 786 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5380
HpaI GTT'AAC
Cuts at: 2970 2970
Size: 5380
KpnI G_GTAC'C
Cuts at: 2521 2705 2521
Size: 184 5196
MfeI C'AATT_G
Cuts at: 2979 2979
Size: 5380
MluI A'CGCG_T
Cuts at: 5256 5256
Size: 5380
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4494 4678 1305
Size: 3189 184 2007
Fragments arranged by size:
3189 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2018 2381 2615 10
Size: 54 1425 343 186 363 234 2775
Fragments arranged by size:
2775 1425 363 343 234 186 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2381 3338 3697 3856 518
Size: 648 1215 957 359 159 2042
Fragments arranged by size:
2042 1215 957 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2086 2103 2190 2426 2582 2796 3473 4621
Size: 480 17 87 236 156 214 677 1148
Cuts at: 4621 4866 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 480 245 236 214 156
87 17
NaeI GCC'GGC
Cuts at: 2069 2069
Size: 5380
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3889
Fragments arranged by size:
3889 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5380
NgoMIV G'CCGG_C
Cuts at: 2067 2067
Size: 5380
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5380
NotI GC'GGCC_GC
Cuts at: 2827 2827
Size: 5380
NspI r_CATG'y
Cuts at: 1132 1229 2738 4283 1132
Size: 97 1509 1545 2229
Fragments arranged by size:
2229 1545 1509 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5380
PciI A'CATG_T
Cuts at: 4279 4279
Size: 5380
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5380
PfoI T'CCnGG_A
Cuts at: 2604 2604
Size: 5380
PmeI GTTT'AAAC
Cuts at: 2684 2684
Size: 5380
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3449 3481 4962 4994 3449
Size: 32 1481 32 3835
Fragments arranged by size:
3835 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5380
PsiI TTA'TAA
Cuts at: 2950 2950
Size: 5380
PstI C_TGCA'G
Cuts at: 830 2732 830
Size: 1902 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5380
PvuII CAG'CTG
Cuts at: 2426 2582 2426
Size: 156 5224
RsrII CG'GwC_CG
Cuts at: 2185 2185
Size: 5380
SacII CC_GC'GG
Cuts at: 2104 2191 2104
Size: 87 5293
SalI G'TCGA_C
Cuts at: 1936 2722 1936
Size: 786 4594
SbfI CC_TGCA'GG
Cuts at: 2732 2732
Size: 5380
ScaI AGT'ACT
Cuts at: 3628 5340 3628
Size: 1712 3668
SfcI C'TryA_G
Cuts at: 826 1045 2404 2728 3863 4544 4735 826
Size: 219 1359 324 1135 681 191 1471
Fragments arranged by size:
1471 1359 1135 681 324 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2179 2179
Size: 5380
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5380
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5380
SmaI CCC'GGG
Cuts at: 1872 2059 2707 1872
Size: 187 648 4545
Fragments arranged by size:
4545 648 187
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3484 4385 4647 820
Size: 197 926 1541 901 262 1553
Fragments arranged by size:
1553 1541 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3171 493
Size: 2678 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5380
SphI G_CATG'C
Cuts at: 1132 2738 1132
Size: 1606 3774
SspI AAT'ATT
Cuts at: 5 52 3304 5
Size: 47 3252 2081
Fragments arranged by size:
3252 2081 47
StuI AGG'CCT
Cuts at: 1517 2654 1517
Size: 1137 4243
StyI C'CwwG_G
Cuts at: 513 1065 2004 2148 2574 2819 513
Size: 552 939 144 426 245 3074
Fragments arranged by size:
3074 939 552 426 245 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2324 3407 3424 3577 3762 4207 325
Size: 1255 744 1083 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1083 744 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3626 5338 96
Size: 274 80 33 51 1443 1649 1712 138
Fragments arranged by size:
1712 1649 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5340 5372 5340
Size: 32 5348
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5380
XbaI T'CTAG_A
Cuts at: 2716 2716
Size: 5380
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2501 2011
Size: 490 4890
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5380
XmaI C'CCGG_G
Cuts at: 1870 2057 2705 1870
Size: 187 648 4545
Fragments arranged by size:
4545 648 187
XmnI GAAnn'nnTTC
Cuts at: 3509 3509
Size: 5380
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3188 276
Size: 53 83 186 936 1654 2468
Fragments arranged by size:
2468 1654 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII RsrII SacII SalI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI SacI
SanDI SapI SexAI SfoI SrfI SwaI