Restriction map for
pFN21AE1716
(Circular) (Six-base) MAPSORT of: pFN21AE1716.seq Check: 724 from: 1 to: 5365
pFN21AE1716 5365 bp
With 182 enzymes: SgfI *
July 4, 2020 18:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3175 278
Size: 53 83 186 936 1639 2468
Fragments arranged by size:
2468 1639 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2708 1937
Size: 771 4594
Acc65I G'GTAC_C
Cuts at: 2686 2686
Size: 5365
AclI AA'CG_TT
Cuts at: 1563 3492 3865 1563
Size: 1929 373 3063
Fragments arranged by size:
3063 1929 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2216 3428 4812 5080 1632
Size: 584 1212 1384 268 1917
Fragments arranged by size:
1917 1384 1212 584 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5168
AflIII A'CryG_T
Cuts at: 1203 4264 5241 1203
Size: 3061 977 1327
Fragments arranged by size:
3061 1327 977
AgeI A'CCGG_T
Cuts at: 4196 4196
Size: 5365
AhdI GACnn_n'nnGTC
Cuts at: 4094 4094
Size: 5365
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5365
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2579 1184
Size: 1395 3970
AlwNI CAG_nnn'CTG
Cuts at: 3108 4680 3108
Size: 1572 3793
ApaI G_GGCC'C
Cuts at: 2607 2607
Size: 5365
ApaLI G'TGCA_C
Cuts at: 3422 4578 3422
Size: 1156 4209
ApoI r'AATT_y
Cuts at: 1493 2305 2674 2900 3052 5161 1493
Size: 812 369 226 152 2109 1697
Fragments arranged by size:
2109 1697 812 369 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3919 160
Size: 872 2887 1606
Fragments arranged by size:
2887 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5365
AvaI C'yCGr_G
Cuts at: 1870 1943 2600 2690 1870
Size: 73 657 90 4545
Fragments arranged by size:
4545 657 90 73
BamHI G'GATC_C
Cuts at: 2695 3062 2695
Size: 367 4998
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2686 4141 618
Size: 325 217 627 899 1455 1842
Fragments arranged by size:
1842 1455 899 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2607 2684 1391
Size: 1216 77 4072
Fragments arranged by size:
4072 1216 77
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4394
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3556 3590 1903
Size: 34 50 34 1535 34 3678
Fragments arranged by size:
3678 1535 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2367 3257 4467 2367
Size: 890 1210 3265
Fragments arranged by size:
3265 1210 890
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5365
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3976 136
Size: 107 122 71 631 605 2304 1525
Fragments arranged by size:
2304 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5360 5360
Size: 5365
BlpI GC'TnA_GC
Cuts at: 2782 2782
Size: 5365
Bme1580I G_kGCm'C
Cuts at: 1790 2607 3426 4582 1790
Size: 817 819 1156 2573
Fragments arranged by size:
2573 1156 819 817
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5365
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3096 4054 442
Size: 416 53 83 399 24 1679 958 1753
Fragments arranged by size:
1753 1679 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5365
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2285 4025 1239
Size: 1046 1740 2579
Fragments arranged by size:
2579 1740 1046
Bpu10I CC'TnA_GC
Cuts at: 2009 2117 2009
Size: 108 5257
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2131 3490 4355 4653 2131
Size: 1359 865 298 2843
Fragments arranged by size:
2843 1359 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4028 882
Size: 617 2529 2219
Fragments arranged by size:
2529 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3156 493
Size: 711 1952 2702
Fragments arranged by size:
2702 1952 711
BsaBI GATnn'nnATC
Cuts at: 5359 5359
Size: 5365
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3172 3554 4189 275
Size: 53 83 186 936 1639 382 635 1451
Fragments arranged by size:
1639 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3798 4196 4470 4617 5028 3798
Size: 398 274 147 411 4135
Fragments arranged by size:
4135 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5140
Fragments arranged by size:
5140 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2352 2557 2566 1186
Size: 1166 205 9 3985
Fragments arranged by size:
3985 1166 205 9
BseYI C'CCAG_C
Cuts at: 2223 4568 5064 2223
Size: 2345 496 2524
Fragments arranged by size:
2524 2345 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2549 1163
Size: 573 813 3979
Fragments arranged by size:
3979 813 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2815 3576 4604 1540
Size: 275 186 814 761 1028 2301
Fragments arranged by size:
2301 1028 814 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3426 3511 4582 3426
Size: 85 1071 4209
Fragments arranged by size:
4209 1071 85
BsiWI C'GTAC_G
Cuts at: 2097 2097
Size: 5365
BsmI GAATG_Cn'
Cuts at: 2876 2969 2876
Size: 93 5272
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2607 2684 3426 3511 4582 1391
Size: 399 817 77 742 85 1071 2174
Fragments arranged by size:
2174 1071 817 742 399 85 77
BspEI T'CCGG_A
Cuts at: 5028 5028
Size: 5365
BspHI T'CATG_A
Cuts at: 3254 4984 3254
Size: 1730 3635
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2720 844
Size: 1876 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3252 1394
Size: 429 1429 3507
Fragments arranged by size:
3507 1429 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2284 2395 3860 4034 65
Size: 1183 1036 111 1465 174 1396
Fragments arranged by size:
1465 1396 1183 1036 174 111
BsrFI r'CCGG_y
Cuts at: 1700 1810 4009 4196 1700
Size: 110 2199 187 2869
Fragments arranged by size:
2869 2199 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5365
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5365
BssSI C'ACGA_G
Cuts at: 3425 4437 3425
Size: 1012 4353
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5365
BstBI TT'CG_AA
Cuts at: 4169 4169
Size: 5365
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5365
BstYI r'GATC_y
Cuts at: 1972 2695 3062 3447 3464 4916 5002 5014 5360
Size: 723 367 385 17 1452 86 12 346
Cuts at: 5360 1972
Size: 1977
Fragments arranged by size:
1977 1452 723 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4813
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4626
Fragments arranged by size:
4626 683 56
BtsI GCAGTG_nn'
Cuts at: 2881 3675 3695 2881
Size: 794 20 4551
Fragments arranged by size:
4551 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5365
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5330
DraI TTT'AAA
Cuts at: 2669 2669
Size: 5365
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2108 4372 809
Size: 507 792 2264 1802
Fragments arranged by size:
2264 1802 792 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2812 3701 5330
Size: 54 1425 50 275 18 982 889 1629
Cuts at: 5330 8
Size: 43
Fragments arranged by size:
1629 1425 982 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2812 1537
Size: 275 1000 4090
Fragments arranged by size:
4090 1000 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2147 2378 3295 1353
Size: 794 231 917 3423
Fragments arranged by size:
3423 917 794 231
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3952 4326 4472 387
Size: 1064 138 2363 374 146 1280
Fragments arranged by size:
2363 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2216 2285 3428 4025 4812 5080 1239
Size: 393 584 69 1143 597 787 268 1524
Fragments arranged by size:
1524 1143 787 597 584 393 268 69
EcoRI G'AATT_C
Cuts at: 2674 3052 2674
Size: 378 4987
FspI TGC'GCA
Cuts at: 3871 3871
Size: 5365
HaeII r_GCGC'y
Cuts at: 1678 4512 1678
Size: 2834 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4006 4038 4080 4112 1373
Size: 32 135 32 2434 32 42 32 2626
Fragments arranged by size:
2626 2434 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2113 2317 2709 2955 669
Size: 988 281 175 204 392 246 3079
Fragments arranged by size:
3079 988 392 281 246 204 175
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5365
HpaI GTT'AAC
Cuts at: 2317 2955 2317
Size: 638 4727
KpnI G_GTAC'C
Cuts at: 2690 2690
Size: 5365
MfeI C'AATT_G
Cuts at: 2964 2964
Size: 5365
MluI A'CGCG_T
Cuts at: 5241 5241
Size: 5365
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4479 4663 1305
Size: 3174 184 2007
Fragments arranged by size:
3174 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3543
Fragments arranged by size:
3543 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2582 3323 3682 3841 518
Size: 648 1416 741 359 159 2042
Fragments arranged by size:
2042 1416 741 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2781 3458 4606 4851 1606
Size: 1175 677 1148 245 2120
Fragments arranged by size:
2120 1175 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4813
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5365
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5365
NotI GC'GGCC_GC
Cuts at: 2812 2812
Size: 5365
NspI r_CATG'y
Cuts at: 1132 1229 2723 4268 1132
Size: 97 1494 1545 2229
Fragments arranged by size:
2229 1545 1494 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5365
PciI A'CATG_T
Cuts at: 4264 4264
Size: 5365
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5365
PfoI T'CCnGG_A
Cuts at: 2443 2443
Size: 5365
PmeI GTTT'AAAC
Cuts at: 2669 2669
Size: 5365
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3434 3466 4947 4979 3434
Size: 32 1481 32 3820
Fragments arranged by size:
3820 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5365
PsiI TTA'TAA
Cuts at: 2935 2935
Size: 5365
PspOMI G'GGCC_C
Cuts at: 2603 2603
Size: 5365
PstI C_TGCA'G
Cuts at: 830 2717 830
Size: 1887 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5365
SalI G'TCGA_C
Cuts at: 1936 2707 1936
Size: 771 4594
SapI GCTCTTCn'nnn_
Cuts at: 2378 2378
Size: 5365
SbfI CC_TGCA'GG
Cuts at: 2717 2717
Size: 5365
ScaI AGT'ACT
Cuts at: 3613 5325 3613
Size: 1712 3653
SfcI C'TryA_G
Cuts at: 826 1045 2500 2713 3848 4529 4720 826
Size: 219 1455 213 1135 681 191 1471
Fragments arranged by size:
1471 1455 1135 681 219 213 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5365
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5365
SmaI CCC'GGG
Cuts at: 1872 2602 2692 1872
Size: 730 90 4545
Fragments arranged by size:
4545 730 90
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2146 3469 4370 4632 820
Size: 197 926 203 1323 901 262 1553
Fragments arranged by size:
1553 1323 926 901 262 203 197
SnaBI TAC'GTA
Cuts at: 493 3156 493
Size: 2663 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5365
SphI G_CATG'C
Cuts at: 1132 2723 1132
Size: 1591 3774
SspI AAT'ATT
Cuts at: 5 52 3289 5
Size: 47 3237 2081
Fragments arranged by size:
3237 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5365
StyI C'CwwG_G
Cuts at: 513 1065 2804 513
Size: 552 1739 3074
Fragments arranged by size:
3074 1739 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2455 2527 3392 3409 3562 3747 4192
Size: 1255 875 72 865 17 153 185 445
Cuts at: 4192 325
Size: 1498
Fragments arranged by size:
1498 1255 875 865 445 185 153 72
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3611 5323 96
Size: 274 80 33 51 1443 1634 1712 138
Fragments arranged by size:
1712 1634 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5325 5357 5325
Size: 32 5333
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5365
XbaI T'CTAG_A
Cuts at: 2701 2701
Size: 5365
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5365
XmaI C'CCGG_G
Cuts at: 1870 2600 2690 1870
Size: 730 90 4545
Fragments arranged by size:
4545 730 90
XmnI GAAnn'nnTTC
Cuts at: 3494 3494
Size: 5365
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3173 276
Size: 53 83 186 936 1639 2468
Fragments arranged by size:
2468 1639 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI