Restriction map for
pFN21AE1713
(Circular) (Six-base) MAPSORT of: pFN21AE1713.seq Check: 1593 from: 1 to: 5353
pFN21AE1713 5353 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3163 278
Size: 53 83 186 936 1627 2468
Fragments arranged by size:
2468 1627 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2696 1937
Size: 759 4594
Acc65I G'GTAC_C
Cuts at: 2301 2674 2301
Size: 373 4980
AclI AA'CG_TT
Cuts at: 1563 3480 3853 1563
Size: 1917 373 3063
Fragments arranged by size:
3063 1917 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2327 3416 4800 5068 1632
Size: 695 1089 1384 268 1917
Fragments arranged by size:
1917 1384 1089 695 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5156
AflIII A'CryG_T
Cuts at: 1203 2562 4252 5229 1203
Size: 1359 1690 977 1327
Fragments arranged by size:
1690 1359 1327 977
AgeI A'CCGG_T
Cuts at: 4184 4184
Size: 5353
AhdI GACnn_n'nnGTC
Cuts at: 4082 4082
Size: 5353
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5353
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5353
AlwNI CAG_nnn'CTG
Cuts at: 2338 3096 4668 2338
Size: 758 1572 3023
Fragments arranged by size:
3023 1572 758
ApaI G_GGCC'C
Cuts at: 2100 2100
Size: 5353
ApaLI G'TGCA_C
Cuts at: 2105 2288 3410 4566 2105
Size: 183 1122 1156 2892
Fragments arranged by size:
2892 1156 1122 183
ApoI r'AATT_y
Cuts at: 1493 2662 2888 3040 5149 1493
Size: 1169 226 152 2109 1697
Fragments arranged by size:
2109 1697 1169 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3907 160
Size: 872 2875 1606
Fragments arranged by size:
2875 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5353
AvaI C'yCGr_G
Cuts at: 1870 1943 2678 1870
Size: 73 735 4545
Fragments arranged by size:
4545 735 73
BamHI G'GATC_C
Cuts at: 2037 2683 3050 2037
Size: 646 367 4340
Fragments arranged by size:
4340 646 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2070 2139 2301 2315 2674
Size: 325 217 627 283 69 162 14 359
Cuts at: 2674 4129 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 359 325 283 217 162
69 14
BanII G_rGCy'C
Cuts at: 1391 2100 2169 2672 1391
Size: 709 69 503 4072
Fragments arranged by size:
4072 709 503 69
BbeI G_GCGC'C
Cuts at: 2074 2319 2074
Size: 245 5108
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2364 928
Size: 971 465 3917
Fragments arranged by size:
3917 971 465
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3544 3578 1903
Size: 34 50 34 1523 34 3678
Fragments arranged by size:
3678 1523 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3245 4455 3245
Size: 1210 4143
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5353
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2391 3964 136
Size: 107 122 71 631 605 719 1573 1525
Fragments arranged by size:
1573 1525 719 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5348 5348
Size: 5353
BlpI GC'TnA_GC
Cuts at: 2770 2770
Size: 5353
Bme1580I G_kGCm'C
Cuts at: 1790 2100 2109 2292 3414 4570 1790
Size: 310 9 183 1122 1156 2573
Fragments arranged by size:
2573 1156 1122 310 183 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5353
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3084 4042 442
Size: 416 53 83 399 24 1667 958 1753
Fragments arranged by size:
1753 1667 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5353
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2155 2264 2600 2642 4013 1239
Size: 916 109 336 42 1371 2579
Fragments arranged by size:
2579 1371 916 336 109 42
Bpu10I CC'TnA_GC
Cuts at: 2438 2438
Size: 5353
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3478 4343 4641 3478
Size: 865 298 4190
Fragments arranged by size:
4190 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2437 2576 4016 882
Size: 617 938 139 1440 2219
Fragments arranged by size:
2219 1440 938 617 139
BsaAI yAC'GTr
Cuts at: 493 1204 2032 2104 3144 493
Size: 711 828 72 1040 2702
Fragments arranged by size:
2702 1040 828 711 72
BsaBI GATnn'nnATC
Cuts at: 5347 5347
Size: 5353
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2071 2202 2316 3160
Size: 53 83 186 936 538 131 114 844
Cuts at: 3160 3542 4177 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 844 635 538 382 186 131
114 83 53
BsaWI w'CCGG_w
Cuts at: 2615 3786 4184 4458 4605 5016 2615
Size: 1171 398 274 147 411 2952
Fragments arranged by size:
2952 1171 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2002 2032 2153 2183 1178
Size: 30 165 30 599 30 121 30 4348
Fragments arranged by size:
4348 599 165 121 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2387 2390 2540 2647 1186
Size: 1201 3 150 107 3892
Fragments arranged by size:
3892 1201 150 107 3
BseYI C'CCAG_C
Cuts at: 2115 2639 4556 5052 2115
Size: 524 1917 496 2416
Fragments arranged by size:
2416 1917 524 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4780
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2803 3564 4592 1540
Size: 275 186 802 761 1028 2301
Fragments arranged by size:
2301 1028 802 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2109 2292 3414 3499 4570 2109
Size: 183 1122 85 1071 2892
Fragments arranged by size:
2892 1122 1071 183 85
BsmI GAATG_Cn'
Cuts at: 2864 2957 2864
Size: 93 5260
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2109 2169 2292 2672 3414 3499
Size: 399 310 9 60 123 380 742 85
Cuts at: 3499 4570 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 380 310 123 85
60 9
BspEI T'CCGG_A
Cuts at: 5016 5016
Size: 5353
BspHI T'CATG_A
Cuts at: 3242 4972 3242
Size: 1730 3623
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2708 844
Size: 1864 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2013 3240 1394
Size: 429 190 1227 3507
Fragments arranged by size:
3507 1227 429 190
BsrDI GCAATG_nn'
Cuts at: 65 1248 3848 4022 65
Size: 1183 2600 174 1396
Fragments arranged by size:
2600 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2045 3997 4184 1700
Size: 110 235 1952 187 2869
Fragments arranged by size:
2869 1952 235 187 110
BsrGI T'GTAC_A
Cuts at: 96 2145 2463 96
Size: 2049 318 2986
Fragments arranged by size:
2986 2049 318
BssHII G'CGCG_C
Cuts at: 1925 2214 2229 1925
Size: 289 15 5049
Fragments arranged by size:
5049 289 15
BssSI C'ACGA_G
Cuts at: 3413 4425 3413
Size: 1012 4341
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2239 2296 2338 794
Size: 1445 57 42 3809
Fragments arranged by size:
3809 1445 57 42
BstBI TT'CG_AA
Cuts at: 4157 4157
Size: 5353
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5353
BstYI r'GATC_y
Cuts at: 1972 2037 2379 2683 3050 3435 3452 4904 4990
Size: 65 342 304 367 385 17 1452 86
Cuts at: 4990 5002 5348 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 342 304 86
65 17 12
Bsu36I CC'TnA_GG
Cuts at: 2532 2540 2532
Size: 8 5345
BtgI C'CryG_G
Cuts at: 513 1065 2061 2161 2271 2514 513
Size: 552 996 100 110 243 3352
Fragments arranged by size:
3352 996 552 243 110 100
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4614
Fragments arranged by size:
4614 683 56
BtsI GCAGTG_nn'
Cuts at: 2449 2869 3663 3683 2449
Size: 420 794 20 4119
Fragments arranged by size:
4119 794 420 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5353
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5318
DraI TTT'AAA
Cuts at: 2657 2657
Size: 5353
DraIII CAC_nnn'GTG
Cuts at: 2296 2296
Size: 5353
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4360 809
Size: 507 3044 1802
Fragments arranged by size:
3044 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2058 2064
Size: 54 1425 50 275 18 213 15 6
Cuts at: 2064 2274 2800 3689 5318 8
Size: 210 526 889 1629 43
Fragments arranged by size:
1629 1425 889 526 275 213 210 54
50 43 18 15 6
EagI C'GGCC_G
Cuts at: 1537 1812 2800 1537
Size: 275 988 4090
Fragments arranged by size:
4090 988 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2575 3283 1353
Size: 1222 708 3423
Fragments arranged by size:
3423 1222 708
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2120 2372 3940 4314 4460 387
Size: 1064 138 531 252 1568 374 146 1280
Fragments arranged by size:
1568 1280 1064 531 374 252 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2155 2264 2327 2600 2642 3416 4013
Size: 393 523 109 63 273 42 774 597
Cuts at: 4013 4800 5068 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 774 597 523 393 273 268
109 63 42
EcoO109I rG'GnC_Cy
Cuts at: 2096 2097 2536 2096
Size: 1 439 4913
Fragments arranged by size:
4913 439 1
EcoRI G'AATT_C
Cuts at: 2662 3040 2662
Size: 378 4975
FseI GG_CCGG'CC
Cuts at: 2049 2049
Size: 5353
FspI TGC'GCA
Cuts at: 2186 3859 2186
Size: 1673 3680
HaeII r_GCGC'y
Cuts at: 1678 2074 2319 4500 1678
Size: 396 245 2181 2531
Fragments arranged by size:
2531 2181 396 245
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2552 2584 3994 4026 4068
Size: 32 135 32 980 32 1410 32 42
Cuts at: 4068 4100 1373
Size: 32 2626
Fragments arranged by size:
2626 1410 980 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2697 2943 669
Size: 988 281 759 246 3079
Fragments arranged by size:
3079 988 759 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5353
HpaI GTT'AAC
Cuts at: 2943 2943
Size: 5353
KasI G'GCGC_C
Cuts at: 2070 2315 2070
Size: 245 5108
KpnI G_GTAC'C
Cuts at: 2305 2678 2305
Size: 373 4980
MfeI C'AATT_G
Cuts at: 2952 2952
Size: 5353
MluI A'CGCG_T
Cuts at: 5229 5229
Size: 5353
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4467 4651 1305
Size: 3162 184 2007
Fragments arranged by size:
3162 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2276 10
Size: 54 1425 343 444 3087
Fragments arranged by size:
3087 1425 444 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3311 3670 3829 518
Size: 648 2145 359 159 2042
Fragments arranged by size:
2145 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2175 2455 2461 2769 3446 4594 4839 1606
Size: 569 280 6 308 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 569 308 280 245 6
NaeI GCC'GGC
Cuts at: 2047 2047
Size: 5353
NarI GG'CG_CC
Cuts at: 2071 2316 2071
Size: 245 5108
NcoI C'CATG_G
Cuts at: 513 1065 2514 513
Size: 552 1449 3352
Fragments arranged by size:
3352 1449 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5353
NgoMIV G'CCGG_C
Cuts at: 2045 2045
Size: 5353
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5353
NotI GC'GGCC_GC
Cuts at: 2800 2800
Size: 5353
NspI r_CATG'y
Cuts at: 1132 1229 2566 2711 4256 1132
Size: 97 1337 145 1545 2229
Fragments arranged by size:
2229 1545 1337 145 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5353
PciI A'CATG_T
Cuts at: 2562 4252 2562
Size: 1690 3663
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5353
PmeI GTTT'AAAC
Cuts at: 2657 2657
Size: 5353
PmlI CAC'GTG
Cuts at: 2104 2104
Size: 5353
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3422 3454 4935 4967 3422
Size: 32 1481 32 3808
Fragments arranged by size:
3808 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2536 2536
Size: 5353
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5353
PsiI TTA'TAA
Cuts at: 2923 2923
Size: 5353
PspOMI G'GGCC_C
Cuts at: 2096 2096
Size: 5353
PstI C_TGCA'G
Cuts at: 830 2705 830
Size: 1875 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5353
PvuII CAG'CTG
Cuts at: 2175 2461 2175
Size: 286 5067
RsrII CG'GwC_CG
Cuts at: 2268 2268
Size: 5353
SalI G'TCGA_C
Cuts at: 1936 2695 1936
Size: 759 4594
SbfI CC_TGCA'GG
Cuts at: 2705 2705
Size: 5353
ScaI AGT'ACT
Cuts at: 2348 3601 5313 2348
Size: 1253 1712 2388
Fragments arranged by size:
2388 1712 1253
SfcI C'TryA_G
Cuts at: 826 1045 2701 3836 4517 4708 826
Size: 219 1656 1135 681 191 1471
Fragments arranged by size:
1656 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2072 2317 2072
Size: 245 5108
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5353
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5353
SmaI CCC'GGG
Cuts at: 1872 2680 1872
Size: 808 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3457 4358 4620 820
Size: 197 926 1514 901 262 1553
Fragments arranged by size:
1553 1514 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3144 493
Size: 2651 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5353
SphI G_CATG'C
Cuts at: 1132 2711 1132
Size: 1579 3774
SspI AAT'ATT
Cuts at: 5 52 3277 5
Size: 47 3225 2081
Fragments arranged by size:
3225 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5353
StyI C'CwwG_G
Cuts at: 513 1065 2514 2792 513
Size: 552 1449 278 3074
Fragments arranged by size:
3074 1449 552 278
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2600 3380 3397 3550 3735 4180 325
Size: 1255 1020 780 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1020 780 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2145 2346 2463
Size: 274 80 33 51 1443 168 201 117
Cuts at: 2463 3599 5311 96
Size: 1136 1712 138
Fragments arranged by size:
1712 1443 1136 274 201 168 138 117
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2002 2034 2153 2185 5313 5345 2002
Size: 32 119 32 3128 32 2010
Fragments arranged by size:
3128 2010 119 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2201 1316
Size: 885 4468
XbaI T'CTAG_A
Cuts at: 2689 2689
Size: 5353
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2512 2512
Size: 5353
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5353
XmaI C'CCGG_G
Cuts at: 1870 2678 1870
Size: 808 4545
XmnI GAAnn'nnTTC
Cuts at: 3482 3482
Size: 5353
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3161 276
Size: 53 83 186 936 1627 2468
Fragments arranged by size:
2468 1627 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII RsrII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV
FalI FspAI NruI NsiI PacI PfoI PspXI PsrI
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI