Restriction map for
pFN21AE1706
(Circular) (Six-base) MAPSORT of: pFN21AE1706.seq Check: 9219 from: 1 to: 5338
pFN21AE1706 5338 bp
With 182 enzymes: SgfI *
June 19, 2019 18:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2053 2190 2241 2053
Size: 137 51 5150
Fragments arranged by size:
5150 137 51
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3148 278
Size: 53 83 186 936 1612 2468
Fragments arranged by size:
2468 1612 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2681 1937
Size: 744 4594
Acc65I G'GTAC_C
Cuts at: 2659 2659
Size: 5338
AclI AA'CG_TT
Cuts at: 1563 3465 3838 1563
Size: 1902 373 3063
Fragments arranged by size:
3063 1902 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3401 4785 5053 1632
Size: 1769 1384 268 1917
Fragments arranged by size:
1917 1769 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5141
AflIII A'CryG_T
Cuts at: 1203 4237 5214 1203
Size: 3034 977 1327
Fragments arranged by size:
3034 1327 977
AgeI A'CCGG_T
Cuts at: 4169 4169
Size: 5338
AhdI GACnn_n'nnGTC
Cuts at: 4067 4067
Size: 5338
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5338
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5338
AlwNI CAG_nnn'CTG
Cuts at: 2242 3081 4653 2242
Size: 839 1572 2927
Fragments arranged by size:
2927 1572 839
ApaLI G'TGCA_C
Cuts at: 3395 4551 3395
Size: 1156 4182
ApoI r'AATT_y
Cuts at: 1493 2300 2561 2647 2873 3025 5134 1493
Size: 807 261 86 226 152 2109 1697
Fragments arranged by size:
2109 1697 807 261 226 152 86
AseI AT'TA_AT
Cuts at: 160 1032 3892 160
Size: 872 2860 1606
Fragments arranged by size:
2860 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5338
AvaI C'yCGr_G
Cuts at: 1870 1943 2663 1870
Size: 73 720 4545
Fragments arranged by size:
4545 720 73
BamHI G'GATC_C
Cuts at: 2590 2668 3035 2590
Size: 78 367 4893
Fragments arranged by size:
4893 367 78
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2264 2537 2659 4114 618
Size: 325 217 627 477 273 122 1455 1842
Fragments arranged by size:
1842 1455 627 477 325 273 217 122
BanII G_rGCy'C
Cuts at: 1391 2657 1391
Size: 1266 4072
BbeI G_GCGC'C
Cuts at: 2541 2541
Size: 5338
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4367
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3529 3563 1903
Size: 34 1592 34 3678
Fragments arranged by size:
3678 1592 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3230 4440 3230
Size: 1210 4128
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5338
BfrBI ATG'CAT
Cuts at: 2415 2415
Size: 5338
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2049 2223 3949
Size: 107 122 71 631 605 377 174 1726
Cuts at: 3949 136
Size: 1525
Fragments arranged by size:
1726 1525 631 605 377 174 122 107
71
BglII A'GATC_T
Cuts at: 5333 5333
Size: 5338
BlpI GC'TnA_GC
Cuts at: 2755 2755
Size: 5338
Bme1580I G_kGCm'C
Cuts at: 1790 2267 3399 4555 1790
Size: 477 1132 1156 2573
Fragments arranged by size:
2573 1156 1132 477
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5338
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3069 4027 442
Size: 416 53 83 399 24 1652 958 1753
Fragments arranged by size:
1753 1652 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2066 3998 1239
Size: 827 1932 2579
Fragments arranged by size:
2579 1932 827
Bpu10I CC'TnA_GC
Cuts at: 2014 2014
Size: 5338
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3463 4328 4626 3463
Size: 865 298 4175
Fragments arranged by size:
4175 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2357 4001 882
Size: 617 858 1644 2219
Fragments arranged by size:
2219 1644 858 617
BsaAI yAC'GTr
Cuts at: 493 1204 3129 493
Size: 711 1925 2702
Fragments arranged by size:
2702 1925 711
BsaBI GATnn'nnATC
Cuts at: 5332 5332
Size: 5338
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2538 3145 3527 4162
Size: 53 83 186 936 1005 607 382 635
Cuts at: 4162 275
Size: 1451
Fragments arranged by size:
1451 1005 936 635 607 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3771 4169 4443 4590 5001 3771
Size: 398 274 147 411 4108
Fragments arranged by size:
4108 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5113
Fragments arranged by size:
5113 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5338
BseYI C'CCAG_C
Cuts at: 2113 4541 5037 2113
Size: 2428 496 2414
Fragments arranged by size:
2428 2414 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2168 2279 1163
Size: 573 432 111 4222
Fragments arranged by size:
4222 573 432 111
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2105 2788 3549 4577 1540
Size: 275 186 104 683 761 1028 2301
Fragments arranged by size:
2301 1028 761 683 275 186 104
BsiHKAI G_wGCw'C
Cuts at: 3399 3484 4555 3399
Size: 85 1071 4182
Fragments arranged by size:
4182 1071 85
BsmI GAATG_Cn'
Cuts at: 2417 2849 2942 2417
Size: 432 93 4813
Fragments arranged by size:
4813 432 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2267 2657 3399 3484 4555 1391
Size: 399 477 390 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 477 399 390 85
BspEI T'CCGG_A
Cuts at: 5001 5001
Size: 5338
BspHI T'CATG_A
Cuts at: 3227 4957 3227
Size: 1730 3608
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2053 2190 2241 2484 2693 844
Size: 1209 137 51 243 209 3489
Fragments arranged by size:
3489 1209 243 209 137 51
BsrBI CCG'CTC
Cuts at: 1394 1823 2101 2140 3225 1394
Size: 429 278 39 1085 3507
Fragments arranged by size:
3507 1085 429 278 39
BsrDI GCAATG_nn'
Cuts at: 65 1248 3833 4007 65
Size: 1183 2585 174 1396
Fragments arranged by size:
2585 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2202 2222 3982 4169 1700
Size: 110 392 20 1760 187 2869
Fragments arranged by size:
2869 1760 392 187 110 20
BsrGI T'GTAC_A
Cuts at: 96 2613 2631 96
Size: 2517 18 2803
Fragments arranged by size:
2803 2517 18
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5338
BssSI C'ACGA_G
Cuts at: 3398 4410 3398
Size: 1012 4326
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2242 794
Size: 1448 3890
BstBI TT'CG_AA
Cuts at: 4142 4142
Size: 5338
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5338
BstYI r'GATC_y
Cuts at: 1972 2238 2590 2668 3035 3420 3437 4889 4975
Size: 266 352 78 367 385 17 1452 86
Cuts at: 4975 4987 5333 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 352 346 266 86
78 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2149 513
Size: 552 939 145 3702
Fragments arranged by size:
3702 939 552 145
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4599
Fragments arranged by size:
4599 683 56
BtsI GCAGTG_nn'
Cuts at: 2322 2854 3648 3668 2322
Size: 532 794 20 3992
Fragments arranged by size:
3992 794 532 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5338
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5303
DraI TTT'AAA
Cuts at: 2642 2642
Size: 5338
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4345 809
Size: 507 3029 1802
Fragments arranged by size:
3029 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2102 2488 2785
Size: 54 1425 50 275 18 272 386 297
Cuts at: 2785 3674 5303 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 386 297 275 272 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2102 2785 1537
Size: 275 290 683 4090
Fragments arranged by size:
4090 683 290 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2291 2430 3268 1353
Size: 938 139 838 3423
Fragments arranged by size:
3423 938 838 139
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2150 3925 4299 4445 387
Size: 1064 138 561 1775 374 146 1280
Fragments arranged by size:
1775 1280 1064 561 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 3401 3998 4785 5053 1239
Size: 393 434 1335 597 787 268 1524
Fragments arranged by size:
1524 1335 787 597 434 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2620 2620
Size: 5338
EcoRI G'AATT_C
Cuts at: 2300 2647 3025 2300
Size: 347 378 4613
Fragments arranged by size:
4613 378 347
FspI TGC'GCA
Cuts at: 2169 3844 2169
Size: 1675 3663
HaeII r_GCGC'y
Cuts at: 1678 2148 2541 4485 1678
Size: 470 393 1944 2531
Fragments arranged by size:
2531 1944 470 393
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3979 4011 4053 4085 1373
Size: 32 135 32 2407 32 42 32 2626
Fragments arranged by size:
2626 2407 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2682 2928 669
Size: 988 281 744 246 3079
Fragments arranged by size:
3079 988 744 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5338
HpaI GTT'AAC
Cuts at: 2928 2928
Size: 5338
KasI G'GCGC_C
Cuts at: 2537 2537
Size: 5338
KpnI G_GTAC'C
Cuts at: 2663 2663
Size: 5338
MfeI C'AATT_G
Cuts at: 2937 2937
Size: 5338
MluI A'CGCG_T
Cuts at: 5214 5214
Size: 5338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4452 4636 1305
Size: 3147 184 2007
Fragments arranged by size:
3147 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3516
Fragments arranged by size:
3516 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2309 3296 3655 3814 518
Size: 648 1143 987 359 159 2042
Fragments arranged by size:
2042 1143 987 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2110 2151 2173 2754 3431 4579 4824 1606
Size: 504 41 22 581 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 581 504 245 41 22
NaeI GCC'GGC
Cuts at: 2204 2204
Size: 5338
NarI GG'CG_CC
Cuts at: 2538 2538
Size: 5338
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3847
Fragments arranged by size:
3847 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5338
NgoMIV G'CCGG_C
Cuts at: 2202 2202
Size: 5338
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5338
NotI GC'GGCC_GC
Cuts at: 2102 2785 2102
Size: 683 4655
NsiI A_TGCA'T
Cuts at: 2417 2417
Size: 5338
NspI r_CATG'y
Cuts at: 1132 1229 2696 4241 1132
Size: 97 1467 1545 2229
Fragments arranged by size:
2229 1545 1467 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5338
PciI A'CATG_T
Cuts at: 4237 4237
Size: 5338
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2065 2534 1108
Size: 957 469 3912
Fragments arranged by size:
3912 957 469
PmeI GTTT'AAAC
Cuts at: 2642 2642
Size: 5338
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3407 3439 4920 4952 3407
Size: 32 1481 32 3793
Fragments arranged by size:
3793 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2620 2620
Size: 5338
PshAI GACnn'nnGTC
Cuts at: 1613 2349 1613
Size: 736 4602
PsiI TTA'TAA
Cuts at: 2908 2908
Size: 5338
PstI C_TGCA'G
Cuts at: 830 2238 2690 830
Size: 1408 452 3478
Fragments arranged by size:
3478 1408 452
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5338
PvuII CAG'CTG
Cuts at: 2173 2173
Size: 5338
SacII CC_GC'GG
Cuts at: 2152 2152
Size: 5338
SalI G'TCGA_C
Cuts at: 1936 2680 1936
Size: 744 4594
SbfI CC_TGCA'GG
Cuts at: 2690 2690
Size: 5338
ScaI AGT'ACT
Cuts at: 3586 5298 3586
Size: 1712 3626
SfcI C'TryA_G
Cuts at: 826 1045 2234 2686 3821 4502 4693 826
Size: 219 1189 452 1135 681 191 1471
Fragments arranged by size:
1471 1189 1135 681 452 219 191
SfoI GGC'GCC
Cuts at: 2539 2539
Size: 5338
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5338
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5338
SmaI CCC'GGG
Cuts at: 1872 2665 1872
Size: 793 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3442 4343 4605 820
Size: 197 926 1499 901 262 1553
Fragments arranged by size:
1553 1499 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3129 493
Size: 2636 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5338
SphI G_CATG'C
Cuts at: 1132 2696 1132
Size: 1564 3774
SspI AAT'ATT
Cuts at: 5 52 3262 5
Size: 47 3210 2081
Fragments arranged by size:
3210 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5338
StyI C'CwwG_G
Cuts at: 513 1065 2004 2777 513
Size: 552 939 773 3074
Fragments arranged by size:
3074 939 773 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3365 3382 3535 3720 4165 325
Size: 1255 1785 17 153 185 445 1498
Fragments arranged by size:
1785 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2613 2631 3584
Size: 274 80 33 51 1443 636 18 953
Cuts at: 3584 5296 96
Size: 1712 138
Fragments arranged by size:
1712 1443 953 636 274 138 80 51
33 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5298 5330 5298
Size: 32 5306
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5338
XbaI T'CTAG_A
Cuts at: 2674 2674
Size: 5338
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5338
XmaI C'CCGG_G
Cuts at: 1870 2663 1870
Size: 793 4545
XmnI GAAnn'nnTTC
Cuts at: 2601 3467 2601
Size: 866 4472
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3146 276
Size: 53 83 186 936 1612 2468
Fragments arranged by size:
2468 1612 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NruI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI
SfiI SrfI SwaI XcmI