Restriction map for
pFN21AE1701
(Circular) (Six-base) MAPSORT of: pFN21AE1701.seq Check: 2960 from: 1 to: 5323
pFN21AE1701 5323 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2563 2563
Size: 5323
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3133 278
Size: 53 83 186 936 1597 2468
Fragments arranged by size:
2468 1597 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2666 1937
Size: 729 4594
Acc65I G'GTAC_C
Cuts at: 2644 2644
Size: 5323
AclI AA'CG_TT
Cuts at: 1563 3450 3823 1563
Size: 1887 373 3063
Fragments arranged by size:
3063 1887 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2309 3386 4770 5038 1632
Size: 677 1077 1384 268 1917
Fragments arranged by size:
1917 1384 1077 677 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5126
AflIII A'CryG_T
Cuts at: 1203 2107 4222 5199 1203
Size: 904 2115 977 1327
Fragments arranged by size:
2115 1327 977 904
AgeI A'CCGG_T
Cuts at: 4154 4154
Size: 5323
AhdI GACnn_n'nnGTC
Cuts at: 4052 4052
Size: 5323
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5323
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5323
AlwNI CAG_nnn'CTG
Cuts at: 3066 4638 3066
Size: 1572 3751
ApaLI G'TGCA_C
Cuts at: 2269 3380 4536 2269
Size: 1111 1156 3056
Fragments arranged by size:
3056 1156 1111
ApoI r'AATT_y
Cuts at: 1493 2632 2858 3010 5119 1493
Size: 1139 226 152 2109 1697
Fragments arranged by size:
2109 1697 1139 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3877 160
Size: 872 2845 1606
Fragments arranged by size:
2845 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5323
AvaI C'yCGr_G
Cuts at: 1870 1943 2211 2648 1870
Size: 73 268 437 4545
Fragments arranged by size:
4545 437 268 73
BamHI G'GATC_C
Cuts at: 2653 3020 2653
Size: 367 4956
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2644 4099 618
Size: 325 217 627 857 1455 1842
Fragments arranged by size:
1842 1455 857 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2387 2642 1391
Size: 996 255 4072
Fragments arranged by size:
4072 996 255
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4352
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3514 3548 1903
Size: 34 50 34 1493 34 3678
Fragments arranged by size:
3678 1493 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3215 4425 3215
Size: 1210 4113
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5323
BfrBI ATG'CAT
Cuts at: 2049 2049
Size: 5323
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2451 3934 136
Size: 107 122 71 631 605 779 1483 1525
Fragments arranged by size:
1525 1483 779 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5318 5318
Size: 5323
BlpI GC'TnA_GC
Cuts at: 2740 2740
Size: 5323
Bme1580I G_kGCm'C
Cuts at: 1790 2273 3384 4540 1790
Size: 483 1111 1156 2573
Fragments arranged by size:
2573 1156 1111 483
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5323
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3054 4012 442
Size: 416 53 83 399 24 1637 958 1753
Fragments arranged by size:
1753 1637 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5323
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2392 3983 1239
Size: 1153 1591 2579
Fragments arranged by size:
2579 1591 1153
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3448 4313 4611 3448
Size: 865 298 4160
Fragments arranged by size:
4160 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3986 882
Size: 617 2487 2219
Fragments arranged by size:
2487 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3114 493
Size: 711 1910 2702
Fragments arranged by size:
2702 1910 711
BsaBI GATnn'nnATC
Cuts at: 5317 5317
Size: 5323
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3130 3512 4147 275
Size: 53 83 186 936 1597 382 635 1451
Fragments arranged by size:
1597 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3756 4154 4428 4575 4986 3756
Size: 398 274 147 411 4093
Fragments arranged by size:
4093 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2285 2315 2384 2414 1178
Size: 30 165 30 882 30 69 30 4087
Fragments arranged by size:
4087 882 165 69 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2364 2395 1186
Size: 1178 31 4114
Fragments arranged by size:
4114 1178 31
BseYI C'CCAG_C
Cuts at: 2135 4526 5022 2135
Size: 2391 496 2436
Fragments arranged by size:
2436 2391 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4750
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2773 3534 4562 1540
Size: 275 186 772 761 1028 2301
Fragments arranged by size:
2301 1028 772 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2273 2334 2387 3384 3469 4540 2273
Size: 61 53 997 85 1071 3056
Fragments arranged by size:
3056 1071 997 85 61 53
BsmI GAATG_Cn'
Cuts at: 2834 2927 2834
Size: 93 5230
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2273 2334 2387 2642 3384 3469 4540
Size: 399 483 61 53 255 742 85 1071
Cuts at: 4540 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 483 399 255 85 61
53
BspEI T'CCGG_A
Cuts at: 4986 4986
Size: 5323
BspHI T'CATG_A
Cuts at: 3212 4942 3212
Size: 1730 3593
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2563 2678 844
Size: 1719 115 3489
Fragments arranged by size:
3489 1719 115
BsrBI CCG'CTC
Cuts at: 1394 1823 2584 3210 1394
Size: 429 761 626 3507
Fragments arranged by size:
3507 761 626 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3818 3992 65
Size: 1183 2570 174 1396
Fragments arranged by size:
2570 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3967 4154 1700
Size: 110 2157 187 2869
Fragments arranged by size:
2869 2157 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5323
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5323
BssSI C'ACGA_G
Cuts at: 3383 4395 3383
Size: 1012 4311
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5323
BstBI TT'CG_AA
Cuts at: 4127 4127
Size: 5323
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5323
BstYI r'GATC_y
Cuts at: 1972 2653 3020 3405 3422 4874 4960 4972 5318
Size: 681 367 385 17 1452 86 12 346
Cuts at: 5318 1972
Size: 1977
Fragments arranged by size:
1977 1452 681 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4771
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4584
Fragments arranged by size:
4584 683 56
BtsI GCAGTG_nn'
Cuts at: 2447 2484 2839 3633 3653 2447
Size: 37 355 794 20 4117
Fragments arranged by size:
4117 794 355 37 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5323
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5288
DraI TTT'AAA
Cuts at: 2077 2627 2077
Size: 550 4773
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4330 809
Size: 507 3014 1802
Fragments arranged by size:
3014 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2263 2770 3659
Size: 54 1425 50 275 18 433 507 889
Cuts at: 3659 5288 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 507 433 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2770 1537
Size: 275 958 4090
Fragments arranged by size:
4090 958 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3253 1353
Size: 1900 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3910 4284 4430 387
Size: 1064 138 2321 374 146 1280
Fragments arranged by size:
2321 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2385 2385
Size: 5323
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2309 2392 3386 3983 4770 5038 1239
Size: 393 677 83 994 597 787 268 1524
Fragments arranged by size:
1524 994 787 677 597 393 268 83
EcoRI G'AATT_C
Cuts at: 2632 3010 2632
Size: 378 4945
FspI TGC'GCA
Cuts at: 3829 3829
Size: 5323
HaeII r_GCGC'y
Cuts at: 1678 4470 1678
Size: 2792 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2534 2566 3964 3996 4038
Size: 32 135 32 962 32 1398 32 42
Cuts at: 4038 4070 1373
Size: 32 2626
Fragments arranged by size:
2626 1398 962 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2667 2913 669
Size: 988 281 729 246 3079
Fragments arranged by size:
3079 988 729 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5323
HpaI GTT'AAC
Cuts at: 2913 2913
Size: 5323
KpnI G_GTAC'C
Cuts at: 2648 2648
Size: 5323
MfeI C'AATT_G
Cuts at: 2922 2922
Size: 5323
MluI A'CGCG_T
Cuts at: 5199 5199
Size: 5323
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4437 4621 1305
Size: 3132 184 2007
Fragments arranged by size:
3132 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2265 10
Size: 54 1425 343 433 3068
Fragments arranged by size:
3068 1425 433 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2054 3281 3640 3799 518
Size: 648 888 1227 359 159 2042
Fragments arranged by size:
2042 1227 888 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2437 2739 3416 4564 4809 1606
Size: 831 302 677 1148 245 2120
Fragments arranged by size:
2120 1148 831 677 302 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4771
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5323
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5323
NotI GC'GGCC_GC
Cuts at: 2770 2770
Size: 5323
NsiI A_TGCA'T
Cuts at: 2051 2051
Size: 5323
NspI r_CATG'y
Cuts at: 1132 1229 2049 2111 2551 2681 4226 1132
Size: 97 820 62 440 130 1545 2229
Fragments arranged by size:
2229 1545 820 440 130 97 62
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5323
PciI A'CATG_T
Cuts at: 2107 4222 2107
Size: 2115 3208
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5323
PmeI GTTT'AAAC
Cuts at: 2627 2627
Size: 5323
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3392 3424 4905 4937 3392
Size: 32 1481 32 3778
Fragments arranged by size:
3778 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5323
PsiI TTA'TAA
Cuts at: 2893 2893
Size: 5323
PstI C_TGCA'G
Cuts at: 830 2442 2675 830
Size: 1612 233 3478
Fragments arranged by size:
3478 1612 233
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5323
PvuII CAG'CTG
Cuts at: 2437 2437
Size: 5323
SacI G_AGCT'C
Cuts at: 2387 2387
Size: 5323
SalI G'TCGA_C
Cuts at: 1936 2665 1936
Size: 729 4594
SbfI CC_TGCA'GG
Cuts at: 2675 2675
Size: 5323
ScaI AGT'ACT
Cuts at: 2369 3571 5283 2369
Size: 1202 1712 2409
Fragments arranged by size:
2409 1712 1202
SfcI C'TryA_G
Cuts at: 826 1045 2438 2671 3806 4487 4678 826
Size: 219 1393 233 1135 681 191 1471
Fragments arranged by size:
1471 1393 1135 681 233 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5323
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5323
SmaI CCC'GGG
Cuts at: 1872 2650 1872
Size: 778 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3427 4328 4590 820
Size: 197 926 1484 901 262 1553
Fragments arranged by size:
1553 1484 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3114 493
Size: 2621 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5323
SphI G_CATG'C
Cuts at: 1132 2049 2681 1132
Size: 917 632 3774
Fragments arranged by size:
3774 917 632
SspI AAT'ATT
Cuts at: 5 52 3247 5
Size: 47 3195 2081
Fragments arranged by size:
3195 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5323
StyI C'CwwG_G
Cuts at: 513 1065 2762 513
Size: 552 1697 3074
Fragments arranged by size:
3074 1697 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3350 3367 3520 3705 4150 325
Size: 1255 1770 17 153 185 445 1498
Fragments arranged by size:
1770 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2367 3569 5281
Size: 274 80 33 51 1443 390 1202 1712
Cuts at: 5281 96
Size: 138
Fragments arranged by size:
1712 1443 1202 390 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2285 2317 2384 2416 2510 2542 5283 5315 2285
Size: 32 67 32 94 32 2741 32 2293
Fragments arranged by size:
2741 2293 94 67 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5323
XbaI T'CTAG_A
Cuts at: 2659 2659
Size: 5323
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5323
XmaI C'CCGG_G
Cuts at: 1870 2648 1870
Size: 778 4545
XmnI GAAnn'nnTTC
Cuts at: 3452 3452
Size: 5323
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3131 276
Size: 53 83 186 936 1597 2468
Fragments arranged by size:
2468 1597 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI