Restriction map for
pFN21AE1698
(Circular) (Six-base) MAPSORT of: pFN21AE1698.seq Check: 3585 from: 1 to: 5380
pFN21AE1698 5380 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3190 278
Size: 53 83 186 936 1654 2468
Fragments arranged by size:
2468 1654 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2723 1937
Size: 786 4594
Acc65I G'GTAC_C
Cuts at: 2701 2701
Size: 5380
AclI AA'CG_TT
Cuts at: 1563 3507 3880 1563
Size: 1944 373 3063
Fragments arranged by size:
3063 1944 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2568 3443 4827 5095 1632
Size: 936 875 1384 268 1917
Fragments arranged by size:
1917 1384 936 875 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5183
AflIII A'CryG_T
Cuts at: 1203 2229 4279 5256 1203
Size: 1026 2050 977 1327
Fragments arranged by size:
2050 1327 1026 977
AgeI A'CCGG_T
Cuts at: 4211 4211
Size: 5380
AhdI GACnn_n'nnGTC
Cuts at: 4109 4109
Size: 5380
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5380
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5380
AlwNI CAG_nnn'CTG
Cuts at: 2146 3123 4695 2146
Size: 977 1572 2831
Fragments arranged by size:
2831 1572 977
ApaI G_GGCC'C
Cuts at: 2569 2569
Size: 5380
ApaLI G'TGCA_C
Cuts at: 3437 4593 3437
Size: 1156 4224
ApoI r'AATT_y
Cuts at: 1493 2689 2915 3067 5176 1493
Size: 1196 226 152 2109 1697
Fragments arranged by size:
2109 1697 1196 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3934 160
Size: 872 2902 1606
Fragments arranged by size:
2902 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5380
AvaI C'yCGr_G
Cuts at: 1870 1943 2705 1870
Size: 73 762 4545
Fragments arranged by size:
4545 762 73
BamHI G'GATC_C
Cuts at: 2576 2710 3077 2576
Size: 134 367 4879
Fragments arranged by size:
4879 367 134
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2607 2701 4156 618
Size: 325 217 627 820 94 1455 1842
Fragments arranged by size:
1842 1455 820 627 325 217 94
BanII G_rGCy'C
Cuts at: 1391 2091 2261 2569 2699 1391
Size: 700 170 308 130 4072
Fragments arranged by size:
4072 700 308 170 130
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2035 2269 928
Size: 971 136 234 4039
Fragments arranged by size:
4039 971 234 136
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3571 3605 1903
Size: 34 1634 34 3678
Fragments arranged by size:
3678 1634 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3272 4482 3272
Size: 1210 4170
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5380
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2252 2484 3991
Size: 107 122 71 631 605 580 232 1507
Cuts at: 3991 136
Size: 1525
Fragments arranged by size:
1525 1507 631 605 580 232 122 107
71
BglII A'GATC_T
Cuts at: 5375 5375
Size: 5380
BlpI GC'TnA_GC
Cuts at: 2797 2797
Size: 5380
Bme1580I G_kGCm'C
Cuts at: 1790 2569 3441 4597 1790
Size: 779 872 1156 2573
Fragments arranged by size:
2573 1156 872 779
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5380
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2109 3111 4069
Size: 416 53 83 399 24 692 1002 958
Cuts at: 4069 442
Size: 1753
Fragments arranged by size:
1753 1002 958 692 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5380
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2168 2540 4040 1239
Size: 929 372 1500 2579
Fragments arranged by size:
2579 1500 929 372
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2093 2354 3505 4370 4668 2093
Size: 261 1151 865 298 2805
Fragments arranged by size:
2805 1151 865 298 261
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4043 882
Size: 617 2544 2219
Fragments arranged by size:
2544 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2425 3171 493
Size: 711 1221 746 2702
Fragments arranged by size:
2702 1221 746 711
BsaBI GATnn'nnATC
Cuts at: 5374 5374
Size: 5380
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3187 3569 4204 275
Size: 53 83 186 936 1654 382 635 1451
Fragments arranged by size:
1654 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3813 4211 4485 4632 5043 3813
Size: 398 274 147 411 4150
Fragments arranged by size:
4150 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5155
Fragments arranged by size:
5155 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2351 2411 2537 2540 1186
Size: 1165 60 126 3 4026
Fragments arranged by size:
4026 1165 126 60 3
BseYI C'CCAG_C
Cuts at: 2082 2504 4583 5079 2082
Size: 422 2079 496 2383
Fragments arranged by size:
2383 2079 496 422
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2478 1163
Size: 573 742 4065
Fragments arranged by size:
4065 742 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2830 3591 4619 1540
Size: 275 186 829 761 1028 2301
Fragments arranged by size:
2301 1028 829 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2091 3441 3526 4597 2091
Size: 1350 85 1071 2874
Fragments arranged by size:
2874 1350 1071 85
BsmI GAATG_Cn'
Cuts at: 2891 2984 2891
Size: 93 5287
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2261 2569 2699 3441 3526 4597
Size: 399 301 170 308 130 742 85 1071
Cuts at: 4597 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 308 301 170 130
85
BspEI T'CCGG_A
Cuts at: 5043 5043
Size: 5380
BspHI T'CATG_A
Cuts at: 3269 4999 3269
Size: 1730 3650
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2157 2655 2735 844
Size: 1313 498 80 3489
Fragments arranged by size:
3489 1313 498 80
BsrBI CCG'CTC
Cuts at: 1394 1823 3267 1394
Size: 429 1444 3507
Fragments arranged by size:
3507 1444 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3875 4049 65
Size: 1183 2627 174 1396
Fragments arranged by size:
2627 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4024 4211 1700
Size: 110 2214 187 2869
Fragments arranged by size:
2869 2214 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5380
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5380
BssSI C'ACGA_G
Cuts at: 3440 4452 3440
Size: 1012 4368
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5380
BstBI TT'CG_AA
Cuts at: 4184 4184
Size: 5380
BstEII G'GTnAC_C
Cuts at: 1184 2530 1184
Size: 1346 4034
BstXI CCAn_nnnn'nTGG
Cuts at: 2122 2122
Size: 5380
BstYI r'GATC_y
Cuts at: 1972 2576 2710 3077 3462 3479 4931 5017 5029
Size: 604 134 367 385 17 1452 86 12
Cuts at: 5029 5375 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 604 385 367 346 134 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2161 2161
Size: 5380
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4828
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4641
Fragments arranged by size:
4641 683 56
BtsI GCAGTG_nn'
Cuts at: 2173 2393 2896 3690 3710 2173
Size: 220 503 794 20 3843
Fragments arranged by size:
3843 794 503 220 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5380
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5345
DraI TTT'AAA
Cuts at: 2684 2684
Size: 5380
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4387 809
Size: 507 3071 1802
Fragments arranged by size:
3071 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2407 2427 2827
Size: 54 1425 50 275 18 577 20 400
Cuts at: 2827 3716 5345 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 577 400 275 54 50
43 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 2827 1537
Size: 275 1015 4090
Fragments arranged by size:
4090 1015 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2228 2604 2618 3310 1353
Size: 875 376 14 692 3423
Fragments arranged by size:
3423 875 692 376 14
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3967 4341 4487 387
Size: 1064 138 2378 374 146 1280
Fragments arranged by size:
2378 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2089 2089
Size: 5380
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2168 2540 2568 3443 4040 4827 5095
Size: 393 536 372 28 875 597 787 268
Cuts at: 5095 1239
Size: 1524
Fragments arranged by size:
1524 875 787 597 536 393 372 268
28
EcoNI CCTnn'n_nnAGG
Cuts at: 2164 2164
Size: 5380
EcoO109I rG'GnC_Cy
Cuts at: 2350 2565 2350
Size: 215 5165
EcoRI G'AATT_C
Cuts at: 2689 3067 2689
Size: 378 5002
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2059 2059
Size: 5380
FspI TGC'GCA
Cuts at: 2267 3886 2267
Size: 1619 3761
FspAI rTGC'GCAy
Cuts at: 2267 2267
Size: 5380
HaeII r_GCGC'y
Cuts at: 1678 4527 1678
Size: 2849 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4021 4053 4095 4127 1373
Size: 32 135 32 2449 32 42 32 2626
Fragments arranged by size:
2626 2449 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2724 2970 669
Size: 988 281 786 246 3079
Fragments arranged by size:
3079 988 786 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5380
HpaI GTT'AAC
Cuts at: 2970 2970
Size: 5380
KpnI G_GTAC'C
Cuts at: 2705 2705
Size: 5380
MfeI C'AATT_G
Cuts at: 2979 2979
Size: 5380
MluI A'CGCG_T
Cuts at: 5256 5256
Size: 5380
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4494 4678 1305
Size: 3189 184 2007
Fragments arranged by size:
3189 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2409 2429 10
Size: 54 1425 343 577 20 2961
Fragments arranged by size:
2961 1425 577 343 54 20
MslI CAynn'nnrTG
Cuts at: 518 1166 2423 3338 3697 3856 518
Size: 648 1257 915 359 159 2042
Fragments arranged by size:
2042 1257 915 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2075 2082 2287 2445 2796 3473 4621 4866
Size: 469 7 205 158 351 677 1148 245
Cuts at: 4866 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 469 351 245 205 158
7
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4828
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5380
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5380
NotI GC'GGCC_GC
Cuts at: 2827 2827
Size: 5380
NspI r_CATG'y
Cuts at: 1132 1229 2233 2272 2332 2738 4283 1132
Size: 97 1004 39 60 406 1545 2229
Fragments arranged by size:
2229 1545 1004 406 97 60 39
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5380
PciI A'CATG_T
Cuts at: 2229 4279 2229
Size: 2050 3330
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5380
PfoI T'CCnGG_A
Cuts at: 2235 2235
Size: 5380
PmeI GTTT'AAAC
Cuts at: 2684 2684
Size: 5380
PmlI CAC'GTG
Cuts at: 2425 2425
Size: 5380
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3449 3481 4962 4994 3449
Size: 32 1481 32 3835
Fragments arranged by size:
3835 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5380
PsiI TTA'TAA
Cuts at: 2950 2950
Size: 5380
PspOMI G'GGCC_C
Cuts at: 2565 2565
Size: 5380
PstI C_TGCA'G
Cuts at: 830 2652 2732 830
Size: 1822 80 3478
Fragments arranged by size:
3478 1822 80
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5380
PvuII CAG'CTG
Cuts at: 2082 2287 2082
Size: 205 5175
SacI G_AGCT'C
Cuts at: 2091 2091
Size: 5380
SalI G'TCGA_C
Cuts at: 1936 2722 1936
Size: 786 4594
SbfI CC_TGCA'GG
Cuts at: 2732 2732
Size: 5380
ScaI AGT'ACT
Cuts at: 3628 5340 3628
Size: 1712 3668
SfcI C'TryA_G
Cuts at: 826 1045 2648 2728 3863 4544 4735 826
Size: 219 1603 80 1135 681 191 1471
Fragments arranged by size:
1603 1471 1135 681 219 191 80
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5380
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5380
SmaI CCC'GGG
Cuts at: 1872 2707 1872
Size: 835 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2108 2333 3484 4385 4647 820
Size: 197 926 165 225 1151 901 262 1553
Fragments arranged by size:
1553 1151 926 901 262 225 197 165
SnaBI TAC'GTA
Cuts at: 493 3171 493
Size: 2678 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5380
SphI G_CATG'C
Cuts at: 1132 2272 2332 2738 1132
Size: 1140 60 406 3774
Fragments arranged by size:
3774 1140 406 60
SspI AAT'ATT
Cuts at: 5 52 3304 5
Size: 47 3252 2081
Fragments arranged by size:
3252 2081 47
StuI AGG'CCT
Cuts at: 1517 2474 1517
Size: 957 4423
StyI C'CwwG_G
Cuts at: 513 1065 2819 513
Size: 552 1754 3074
Fragments arranged by size:
3074 1754 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3407 3424 3577 3762 4207 325
Size: 1255 1827 17 153 185 445 1498
Fragments arranged by size:
1827 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3626 5338 96
Size: 274 80 33 51 1443 1649 1712 138
Fragments arranged by size:
1712 1649 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5340 5372 5340
Size: 32 5348
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5380
XbaI T'CTAG_A
Cuts at: 2716 2716
Size: 5380
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2079 2079
Size: 5380
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5380
XmaI C'CCGG_G
Cuts at: 1870 2705 1870
Size: 835 4545
XmnI GAAnn'nnTTC
Cuts at: 2042 3509 2042
Size: 1467 3913
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3188 276
Size: 53 83 186 936 1654 2468
Fragments arranged by size:
2468 1654 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV
FseI KasI NaeI NarI NgoMIV NruI NsiI PacI
PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI