Restriction map for
pFN21AE1697
(Circular) (Six-base) MAPSORT of: pFN21AE1697.seq Check: 1248 from: 1 to: 5494
pFN21AE1697 5494 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3304 278
Size: 53 83 186 936 1768 2468
Fragments arranged by size:
2468 1768 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2837 1937
Size: 900 4594
Acc65I G'GTAC_C
Cuts at: 2815 2815
Size: 5494
AclI AA'CG_TT
Cuts at: 1563 3621 3994 1563
Size: 2058 373 3063
Fragments arranged by size:
3063 2058 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2253 3557 4941 5209 1632
Size: 621 1304 1384 268 1917
Fragments arranged by size:
1917 1384 1304 621 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5297
AflIII A'CryG_T
Cuts at: 1203 2337 4393 5370 1203
Size: 1134 2056 977 1327
Fragments arranged by size:
2056 1327 1134 977
AgeI A'CCGG_T
Cuts at: 4325 4325
Size: 5494
AhdI GACnn_n'nnGTC
Cuts at: 4223 4223
Size: 5494
AleI CACnn'nnGTG
Cuts at: 1166 2077 1166
Size: 911 4583
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5494
AlwNI CAG_nnn'CTG
Cuts at: 2254 3237 4809 2254
Size: 983 1572 2939
Fragments arranged by size:
2939 1572 983
ApaLI G'TGCA_C
Cuts at: 2600 3551 4707 2600
Size: 951 1156 3387
Fragments arranged by size:
3387 1156 951
ApoI r'AATT_y
Cuts at: 1493 2803 3029 3181 5290 1493
Size: 1310 226 152 2109 1697
Fragments arranged by size:
2109 1697 1310 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4048 160
Size: 872 3016 1606
Fragments arranged by size:
3016 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5494
AvaI C'yCGr_G
Cuts at: 1870 1943 2658 2819 1870
Size: 73 715 161 4545
Fragments arranged by size:
4545 715 161 73
BamHI G'GATC_C
Cuts at: 2824 3191 2824
Size: 367 5127
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2559 2662 2815 4270 618
Size: 325 217 627 772 103 153 1455 1842
Fragments arranged by size:
1842 1455 772 627 325 217 153 103
BanII G_rGCy'C
Cuts at: 1391 2237 2369 2699 2813 1391
Size: 846 132 330 114 4072
Fragments arranged by size:
4072 846 330 132 114
BbeI G_GCGC'C
Cuts at: 2666 2666
Size: 5494
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2143 2377 928
Size: 971 244 234 4045
Fragments arranged by size:
4045 971 244 234
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3685 3719 1903
Size: 34 1748 34 3678
Fragments arranged by size:
3678 1748 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3386 4596 3386
Size: 1210 4284
BclI T'GATC_A
Cuts at: 1548 2757 1548
Size: 1209 4285
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2360 2592 4105
Size: 107 122 71 631 605 688 232 1513
Cuts at: 4105 136
Size: 1525
Fragments arranged by size:
1525 1513 688 631 605 232 122 107
71
BglII A'GATC_T
Cuts at: 5489 5489
Size: 5494
BlpI GC'TnA_GC
Cuts at: 2231 2911 2231
Size: 680 4814
Bme1580I G_kGCm'C
Cuts at: 1790 2604 3555 4711 1790
Size: 814 951 1156 2573
Fragments arranged by size:
2573 1156 951 814
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5494
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3225 4183 442
Size: 416 53 83 399 24 1808 958 1753
Fragments arranged by size:
1808 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5494
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2462 2648 4154 1239
Size: 1223 186 1506 2579
Fragments arranged by size:
2579 1506 1223 186
Bpu10I CC'TnA_GC
Cuts at: 2698 2698
Size: 5494
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2201 3619 4484 4782 2201
Size: 1418 865 298 2913
Fragments arranged by size:
2913 1418 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2102 4157 882
Size: 617 603 2055 2219
Fragments arranged by size:
2219 2055 617 603
BsaAI yAC'GTr
Cuts at: 493 1204 2320 2533 3285 493
Size: 711 1116 213 752 2702
Fragments arranged by size:
2702 1116 752 711 213
BsaBI GATnn'nnATC
Cuts at: 5488 5488
Size: 5494
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2663 3301 3683 4318
Size: 53 83 186 936 1130 638 382 635
Cuts at: 4318 275
Size: 1451
Fragments arranged by size:
1451 1130 936 638 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3927 4325 4599 4746 5157 3927
Size: 398 274 147 411 4264
Fragments arranged by size:
4264 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2639 2669 1178
Size: 30 165 30 1236 30 4003
Fragments arranged by size:
4003 1236 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2183 2459 2645 2648 1186
Size: 997 276 186 3 4032
Fragments arranged by size:
4032 997 276 186 3
BseYI C'CCAG_C
Cuts at: 2190 2355 2612 2666 4697 5193 2190
Size: 165 257 54 2031 496 2491
Fragments arranged by size:
2491 2031 496 257 165 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2030 1163
Size: 573 294 4627
Fragments arranged by size:
4627 573 294
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2944 3705 4733 1540
Size: 275 186 943 761 1028 2301
Fragments arranged by size:
2301 1028 943 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2604 3555 3640 4711 2604
Size: 951 85 1071 3387
Fragments arranged by size:
3387 1071 951 85
BsmI GAATG_Cn'
Cuts at: 3005 3098 3005
Size: 93 5401
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2237 2369 2604 2699 2813 3555 3640
Size: 399 447 132 235 95 114 742 85
Cuts at: 3640 4711 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 447 399 235 132 114
95 85
BspEI T'CCGG_A
Cuts at: 5157 5157
Size: 5494
BspHI T'CATG_A
Cuts at: 3383 5113 3383
Size: 1730 3764
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2265 2775 2849 844
Size: 1421 510 74 3489
Fragments arranged by size:
3489 1421 510 74
BsrBI CCG'CTC
Cuts at: 1394 1823 3381 1394
Size: 429 1558 3507
Fragments arranged by size:
3507 1558 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3989 4163 65
Size: 1183 2741 174 1396
Fragments arranged by size:
2741 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2179 2553 4138 4325 1700
Size: 110 369 374 1585 187 2869
Fragments arranged by size:
2869 1585 374 369 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5494
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5494
BssSI C'ACGA_G
Cuts at: 3554 4566 3554
Size: 1012 4482
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2227 794
Size: 1433 4061
BstBI TT'CG_AA
Cuts at: 4298 4298
Size: 5494
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5494
BstXI CCAn_nnnn'nTGG
Cuts at: 2440 2440
Size: 5494
BstYI r'GATC_y
Cuts at: 1972 2824 3191 3576 3593 5045 5131 5143 5489
Size: 852 367 385 17 1452 86 12 346
Cuts at: 5489 1972
Size: 1977
Fragments arranged by size:
1977 1452 852 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2021 2563 2742 513
Size: 552 956 542 179 3265
Fragments arranged by size:
3265 956 552 542 179
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4755
Fragments arranged by size:
4755 683 56
BtsI GCAGTG_nn'
Cuts at: 2281 2501 3010 3804 3824 2281
Size: 220 509 794 20 3951
Fragments arranged by size:
3951 794 509 220 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5494
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5459
DraI TTT'AAA
Cuts at: 2798 2798
Size: 5494
DraIII CAC_nnn'GTG
Cuts at: 2227 2227
Size: 5494
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4501 809
Size: 507 3185 1802
Fragments arranged by size:
3185 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2515 2535 2941
Size: 54 1425 50 275 18 685 20 406
Cuts at: 2941 3830 5459 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 685 406 275 54 50
43 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 2941 1537
Size: 275 1129 4090
Fragments arranged by size:
4090 1129 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2336 3424 1353
Size: 983 1088 3423
Fragments arranged by size:
3423 1088 983
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4081 4455 4601 387
Size: 1064 138 2492 374 146 1280
Fragments arranged by size:
2492 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2253 2462 2648 3557 4154 4941 5209
Size: 393 621 209 186 909 597 787 268
Cuts at: 5209 1239
Size: 1524
Fragments arranged by size:
1524 909 787 621 597 393 268 209
186
EcoO109I rG'GnC_Cy
Cuts at: 2194 2458 2194
Size: 264 5230
EcoRI G'AATT_C
Cuts at: 2803 3181 2803
Size: 378 5116
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2167 2167
Size: 5494
FspI TGC'GCA
Cuts at: 2375 4000 2375
Size: 1625 3869
FspAI rTGC'GCAy
Cuts at: 2375 2375
Size: 5494
HaeII r_GCGC'y
Cuts at: 1678 2666 4641 1678
Size: 988 1975 2531
Fragments arranged by size:
2531 1975 988
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4135 4167 4209 4241 1373
Size: 32 135 32 2563 32 42 32 2626
Fragments arranged by size:
2626 2563 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2838 3084 669
Size: 988 281 900 246 3079
Fragments arranged by size:
3079 988 900 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5494
HpaI GTT'AAC
Cuts at: 3084 3084
Size: 5494
KasI G'GCGC_C
Cuts at: 2662 2662
Size: 5494
KpnI G_GTAC'C
Cuts at: 2819 2819
Size: 5494
MfeI C'AATT_G
Cuts at: 3093 3093
Size: 5494
MluI A'CGCG_T
Cuts at: 5370 5370
Size: 5494
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2222 4608 4792 1305
Size: 917 2386 184 2007
Fragments arranged by size:
2386 2007 917 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2537 10
Size: 54 1425 343 705 2967
Fragments arranged by size:
2967 1425 705 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2077 2531 3452 3811 3970 518
Size: 648 911 454 921 359 159 2042
Fragments arranged by size:
2042 921 911 648 454 359 159
MspA1I CmG'CkG
Cuts at: 1606 2190 2359 2395 2565 2910 3587 4735 4980
Size: 584 169 36 170 345 677 1148 245
Cuts at: 4980 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 584 345 245 170 169
36
NaeI GCC'GGC
Cuts at: 2181 2181
Size: 5494
NarI GG'CG_CC
Cuts at: 2663 2663
Size: 5494
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4942
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5494
NgoMIV G'CCGG_C
Cuts at: 2179 2179
Size: 5494
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5494
NotI GC'GGCC_GC
Cuts at: 2941 2941
Size: 5494
NspI r_CATG'y
Cuts at: 1132 1229 2267 2341 2380 2440 2852 4397 1132
Size: 97 1038 74 39 60 412 1545 2229
Fragments arranged by size:
2229 1545 1038 412 97 74 60 39
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5494
PciI A'CATG_T
Cuts at: 2337 4393 2337
Size: 2056 3438
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5494
PfoI T'CCnGG_A
Cuts at: 2343 2343
Size: 5494
PmeI GTTT'AAAC
Cuts at: 2798 2798
Size: 5494
PmlI CAC'GTG
Cuts at: 2533 2533
Size: 5494
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2699 2731 3563 3595 5076 5108 2699
Size: 32 832 32 1481 32 3085
Fragments arranged by size:
3085 1481 832 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2194 2194
Size: 5494
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5494
PsiI TTA'TAA
Cuts at: 3064 3064
Size: 5494
PstI C_TGCA'G
Cuts at: 830 2772 2846 830
Size: 1942 74 3478
Fragments arranged by size:
3478 1942 74
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5494
PvuII CAG'CTG
Cuts at: 2190 2395 2190
Size: 205 5289
SacII CC_GC'GG
Cuts at: 2566 2566
Size: 5494
SalI G'TCGA_C
Cuts at: 1936 2836 1936
Size: 900 4594
SbfI CC_TGCA'GG
Cuts at: 2846 2846
Size: 5494
ScaI AGT'ACT
Cuts at: 3742 5454 3742
Size: 1712 3782
SfcI C'TryA_G
Cuts at: 826 1045 2768 2842 3977 4658 4849 826
Size: 219 1723 74 1135 681 191 1471
Fragments arranged by size:
1723 1471 1135 681 219 191 74
SfoI GGC'GCC
Cuts at: 2664 2664
Size: 5494
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5494
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5494
SmaI CCC'GGG
Cuts at: 1872 2660 2821 1872
Size: 788 161 4545
Fragments arranged by size:
4545 788 161
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2216 3598 4499 4761 820
Size: 197 926 273 1382 901 262 1553
Fragments arranged by size:
1553 1382 926 901 273 262 197
SnaBI TAC'GTA
Cuts at: 493 3285 493
Size: 2792 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5494
SphI G_CATG'C
Cuts at: 1132 2267 2380 2440 2852 1132
Size: 1135 113 60 412 3774
Fragments arranged by size:
3774 1135 412 113 60
SspI AAT'ATT
Cuts at: 5 52 3418 5
Size: 47 3366 2081
Fragments arranged by size:
3366 2081 47
StuI AGG'CCT
Cuts at: 1517 2582 1517
Size: 1065 4429
StyI C'CwwG_G
Cuts at: 513 1065 2933 513
Size: 552 1868 3074
Fragments arranged by size:
3074 1868 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3521 3538 3691 3876 4321 325
Size: 1255 1941 17 153 185 445 1498
Fragments arranged by size:
1941 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3740 5452 96
Size: 274 80 33 51 1443 1763 1712 138
Fragments arranged by size:
1763 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5454 5486 5454
Size: 32 5462
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5494
XbaI T'CTAG_A
Cuts at: 2830 2830
Size: 5494
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5494
XmaI C'CCGG_G
Cuts at: 1870 2658 2819 1870
Size: 788 161 4545
Fragments arranged by size:
4545 788 161
XmnI GAAnn'nnTTC
Cuts at: 2150 3623 2150
Size: 1473 4021
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3302 276
Size: 53 83 186 936 1768 2468
Fragments arranged by size:
2468 1768 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FseI NruI NsiI PacI PspOMI PspXI PsrI
RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI
XcmI