Restriction map for
pFN21AE1696
(Circular) (Six-base) MAPSORT of: pFN21AE1696.seq Check: 3072 from: 1 to: 5323
pFN21AE1696 5323 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2404 2404
Size: 5323
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2477 3133 278
Size: 53 83 186 936 941 656 2468
Fragments arranged by size:
2468 941 936 656 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2666 1937
Size: 729 4594
Acc65I G'GTAC_C
Cuts at: 2644 2644
Size: 5323
AclI AA'CG_TT
Cuts at: 1563 3450 3823 1563
Size: 1887 373 3063
Fragments arranged by size:
3063 1887 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2084 2373 3386 4770 5038 1632
Size: 452 289 1013 1384 268 1917
Fragments arranged by size:
1917 1384 1013 452 289 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5126
AflIII A'CryG_T
Cuts at: 1203 4222 5199 1203
Size: 3019 977 1327
Fragments arranged by size:
3019 1327 977
AgeI A'CCGG_T
Cuts at: 4154 4154
Size: 5323
AhdI GACnn_n'nnGTC
Cuts at: 4052 4052
Size: 5323
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5323
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5323
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2322 2354 2492 2524 2322
Size: 32 138 32 5121
Fragments arranged by size:
5121 138 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2315 3066 4638 2315
Size: 751 1572 3000
Fragments arranged by size:
3000 1572 751
ApaLI G'TGCA_C
Cuts at: 3380 4536 3380
Size: 1156 4167
ApoI r'AATT_y
Cuts at: 1493 2321 2364 2632 2858 3010 5119 1493
Size: 828 43 268 226 152 2109 1697
Fragments arranged by size:
2109 1697 828 268 226 152 43
AseI AT'TA_AT
Cuts at: 160 1032 3877 160
Size: 872 2845 1606
Fragments arranged by size:
2845 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5323
AvaI C'yCGr_G
Cuts at: 1870 1943 2487 2648 1870
Size: 73 544 161 4545
Fragments arranged by size:
4545 544 161 73
BamHI G'GATC_C
Cuts at: 2653 3020 2653
Size: 367 4956
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2416 2644 4099 618
Size: 325 217 627 629 228 1455 1842
Fragments arranged by size:
1842 1455 629 627 325 228 217
BanII G_rGCy'C
Cuts at: 1391 2036 2642 1391
Size: 645 606 4072
Fragments arranged by size:
4072 645 606
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2133 2489 928
Size: 971 234 356 3762
Fragments arranged by size:
3762 971 356 234
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3514 3548 1903
Size: 34 1577 34 3678
Fragments arranged by size:
3678 1577 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3215 4425 3215
Size: 1210 4113
BclI T'GATC_A
Cuts at: 1548 2055 1548
Size: 507 4816
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3934 136
Size: 107 122 71 631 605 2262 1525
Fragments arranged by size:
2262 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5318 5318
Size: 5323
BlpI GC'TnA_GC
Cuts at: 2740 2740
Size: 5323
Bme1580I G_kGCm'C
Cuts at: 1790 3384 4540 1790
Size: 1594 1156 2573
Fragments arranged by size:
2573 1594 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5323
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3054 4012 442
Size: 416 53 83 399 24 1637 958 1753
Fragments arranged by size:
1753 1637 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5323
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3983 1239
Size: 2744 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3448 4313 4611 3448
Size: 865 298 4160
Fragments arranged by size:
4160 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3986 882
Size: 617 2487 2219
Fragments arranged by size:
2487 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2467 3114 493
Size: 711 1263 647 2702
Fragments arranged by size:
2702 1263 711 647
BsaBI GATnn'nnATC
Cuts at: 5317 5317
Size: 5323
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2474 3130 3512 4147
Size: 53 83 186 936 941 656 382 635
Cuts at: 4147 275
Size: 1451
Fragments arranged by size:
1451 941 936 656 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3756 4154 4428 4575 4986 3756
Size: 398 274 147 411 4093
Fragments arranged by size:
4093 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2322 2352 1178
Size: 30 165 30 919 30 4149
Fragments arranged by size:
4149 919 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2417 2441 1186
Size: 1231 24 4068
Fragments arranged by size:
4068 1231 24
BseYI C'CCAG_C
Cuts at: 2213 4526 5022 2213
Size: 2313 496 2514
Fragments arranged by size:
2514 2313 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2480 1163
Size: 573 744 4006
Fragments arranged by size:
4006 744 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2773 3534 4562 1540
Size: 275 186 772 761 1028 2301
Fragments arranged by size:
2301 1028 772 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2036 3384 3469 4540 2036
Size: 1348 85 1071 2819
Fragments arranged by size:
2819 1348 1071 85
BsmI GAATG_Cn'
Cuts at: 2834 2927 2834
Size: 93 5230
BsmBI CGTCTCn'nnnn_
Cuts at: 2465 2465
Size: 5323
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2642 3384 3469 4540 1391
Size: 399 246 606 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 606 399 246 85
BspEI T'CCGG_A
Cuts at: 4986 4986
Size: 5323
BspHI T'CATG_A
Cuts at: 3212 4942 3212
Size: 1730 3593
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2404 2678 844
Size: 1560 274 3489
Fragments arranged by size:
3489 1560 274
BsrBI CCG'CTC
Cuts at: 1394 1823 2407 3210 1394
Size: 429 584 803 3507
Fragments arranged by size:
3507 803 584 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3818 3992 65
Size: 1183 2570 174 1396
Fragments arranged by size:
2570 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3967 4154 1700
Size: 110 2157 187 2869
Fragments arranged by size:
2869 2157 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5323
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5323
BssSI C'ACGA_G
Cuts at: 3383 4395 3383
Size: 1012 4311
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5323
BstBI TT'CG_AA
Cuts at: 4127 4127
Size: 5323
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5323
BstYI r'GATC_y
Cuts at: 1972 2653 3020 3405 3422 4874 4960 4972 5318
Size: 681 367 385 17 1452 86 12 346
Cuts at: 5318 1972
Size: 1977
Fragments arranged by size:
1977 1452 681 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3832
Fragments arranged by size:
3832 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4584
Fragments arranged by size:
4584 683 56
BtsI GCAGTG_nn'
Cuts at: 2369 2839 3633 3653 2369
Size: 470 794 20 4039
Fragments arranged by size:
4039 794 470 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5323
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5288
DraI TTT'AAA
Cuts at: 2602 2627 2602
Size: 25 5298
DraIII CAC_nnn'GTG
Cuts at: 2053 2053
Size: 5323
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4330 809
Size: 507 3014 1802
Fragments arranged by size:
3014 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2770 3659 5288
Size: 54 1425 50 275 18 940 889 1629
Cuts at: 5288 8
Size: 43
Fragments arranged by size:
1629 1425 940 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2770 1537
Size: 275 958 4090
Fragments arranged by size:
4090 958 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2442 3253 1353
Size: 1089 811 3423
Fragments arranged by size:
3423 1089 811
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3910 4284 4430 387
Size: 1064 138 2321 374 146 1280
Fragments arranged by size:
2321 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2034 2034
Size: 5323
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2373 3386 3983 4770 5038 1239
Size: 393 452 289 1013 597 787 268 1524
Fragments arranged by size:
1524 1013 787 597 452 393 289 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2048 2048
Size: 5323
EcoRI G'AATT_C
Cuts at: 2321 2632 3010 2321
Size: 311 378 4634
Fragments arranged by size:
4634 378 311
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2189 2288 2527 2189
Size: 99 239 4985
Fragments arranged by size:
4985 239 99
FspI TGC'GCA
Cuts at: 3829 3829
Size: 5323
HaeII r_GCGC'y
Cuts at: 1678 4470 1678
Size: 2792 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3964 3996 4038 4070 1373
Size: 32 135 32 2392 32 42 32 2626
Fragments arranged by size:
2626 2392 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2667 2913 669
Size: 988 281 729 246 3079
Fragments arranged by size:
3079 988 729 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5323
HpaI GTT'AAC
Cuts at: 2913 2913
Size: 5323
KpnI G_GTAC'C
Cuts at: 2648 2648
Size: 5323
MfeI C'AATT_G
Cuts at: 2922 2922
Size: 5323
MluI A'CGCG_T
Cuts at: 5199 5199
Size: 5323
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4437 4621 1305
Size: 3132 184 2007
Fragments arranged by size:
3132 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3501
Fragments arranged by size:
3501 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3281 3640 3799 518
Size: 648 2115 359 159 2042
Fragments arranged by size:
2115 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2070 2213 2739 3416 4564 4809 1606
Size: 464 143 526 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 526 464 245 143
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3832
Fragments arranged by size:
3832 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5323
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5323
NotI GC'GGCC_GC
Cuts at: 2770 2770
Size: 5323
NspI r_CATG'y
Cuts at: 1132 1229 2681 4226 1132
Size: 97 1452 1545 2229
Fragments arranged by size:
2229 1545 1452 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5323
PciI A'CATG_T
Cuts at: 4222 4222
Size: 5323
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5323
PmeI GTTT'AAAC
Cuts at: 2627 2627
Size: 5323
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2322 2354 3392 3424 4905 4937 2322
Size: 32 1038 32 1481 32 2708
Fragments arranged by size:
2708 1481 1038 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5323
PsiI TTA'TAA
Cuts at: 2893 2893
Size: 5323
PstI C_TGCA'G
Cuts at: 830 2675 830
Size: 1845 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5323
SacI G_AGCT'C
Cuts at: 2036 2036
Size: 5323
SalI G'TCGA_C
Cuts at: 1936 2665 1936
Size: 729 4594
SbfI CC_TGCA'GG
Cuts at: 2675 2675
Size: 5323
ScaI AGT'ACT
Cuts at: 3571 5283 3571
Size: 1712 3611
SfcI C'TryA_G
Cuts at: 826 1045 2671 3806 4487 4678 826
Size: 219 1626 1135 681 191 1471
Fragments arranged by size:
1626 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5323
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5323
SmaI CCC'GGG
Cuts at: 1872 2489 2650 1872
Size: 617 161 4545
Fragments arranged by size:
4545 617 161
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3427 4328 4590 820
Size: 197 926 1484 901 262 1553
Fragments arranged by size:
1553 1484 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3114 493
Size: 2621 2702
SpeI A'CTAG_T
Cuts at: 152 2620 152
Size: 2468 2855
SphI G_CATG'C
Cuts at: 1132 2681 1132
Size: 1549 3774
SspI AAT'ATT
Cuts at: 5 52 3247 5
Size: 47 3195 2081
Fragments arranged by size:
3195 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5323
StyI C'CwwG_G
Cuts at: 513 1065 2004 2037 2762 513
Size: 552 939 33 725 3074
Fragments arranged by size:
3074 939 725 552 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3350 3367 3520 3705 4150 325
Size: 1255 1770 17 153 185 445 1498
Fragments arranged by size:
1770 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3569 5281 96
Size: 274 80 33 51 1443 1592 1712 138
Fragments arranged by size:
1712 1592 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5283 5315 5283
Size: 32 5291
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5323
XbaI T'CTAG_A
Cuts at: 2659 2659
Size: 5323
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5323
XmaI C'CCGG_G
Cuts at: 1870 2487 2648 1870
Size: 617 161 4545
Fragments arranged by size:
4545 617 161
XmnI GAAnn'nnTTC
Cuts at: 2187 2268 2334 3452 2187
Size: 81 66 1118 4058
Fragments arranged by size:
4058 1118 81 66
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2475 3131 276
Size: 53 83 186 936 941 656 2468
Fragments arranged by size:
2468 941 936 656 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI