Restriction map for
pFN21AE1695
(Circular) (Six-base) MAPSORT of: pFN21AE1695.seq Check: 7985 from: 1 to: 5311
pFN21AE1695 5311 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2287 2287
Size: 5311
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3121 278
Size: 53 83 186 936 1585 2468
Fragments arranged by size:
2468 1585 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2654 1937
Size: 717 4594
Acc65I G'GTAC_C
Cuts at: 2632 2632
Size: 5311
AclI AA'CG_TT
Cuts at: 1563 3438 3811 1563
Size: 1875 373 3063
Fragments arranged by size:
3063 1875 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3374 4758 5026 1632
Size: 1742 1384 268 1917
Fragments arranged by size:
1917 1742 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5114
AflIII A'CryG_T
Cuts at: 1203 4210 5187 1203
Size: 3007 977 1327
Fragments arranged by size:
3007 1327 977
AgeI A'CCGG_T
Cuts at: 4142 4142
Size: 5311
AhdI GACnn_n'nnGTC
Cuts at: 4040 4040
Size: 5311
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5311
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5311
AlwNI CAG_nnn'CTG
Cuts at: 3054 4626 3054
Size: 1572 3739
ApaLI G'TGCA_C
Cuts at: 2372 3368 4524 2372
Size: 996 1156 3159
Fragments arranged by size:
3159 1156 996
ApoI r'AATT_y
Cuts at: 1493 2620 2846 2998 5107 1493
Size: 1127 226 152 2109 1697
Fragments arranged by size:
2109 1697 1127 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3865 160
Size: 872 2833 1606
Fragments arranged by size:
2833 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5311
AvaI C'yCGr_G
Cuts at: 1870 1943 2352 2361 2636 1870
Size: 73 409 9 275 4545
Fragments arranged by size:
4545 409 275 73 9
BamHI G'GATC_C
Cuts at: 2641 3008 2641
Size: 367 4944
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2357 2632 4087 618
Size: 325 217 627 570 275 1455 1842
Fragments arranged by size:
1842 1455 627 570 325 275 217
BanII G_rGCy'C
Cuts at: 1391 2177 2630 1391
Size: 786 453 4072
Fragments arranged by size:
4072 786 453
BbeI G_GCGC'C
Cuts at: 2361 2361
Size: 5311
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4340
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2037 2071 3502 3536 1903
Size: 34 50 34 16 34 1431 34 3678
Fragments arranged by size:
3678 1431 50 34 34 34 34 16
BciVI GTATCCnnnnn_n'
Cuts at: 3203 4413 3203
Size: 1210 4101
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5311
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2077 2086 2366
Size: 107 122 71 631 605 405 9 280
Cuts at: 2366 3922 136
Size: 1556 1525
Fragments arranged by size:
1556 1525 631 605 405 280 122 107
71 9
BglII A'GATC_T
Cuts at: 2538 5306 2538
Size: 2768 2543
BlpI GC'TnA_GC
Cuts at: 2136 2728 2136
Size: 592 4719
Bme1580I G_kGCm'C
Cuts at: 1790 2249 2376 3372 4528 1790
Size: 459 127 996 1156 2573
Fragments arranged by size:
2573 1156 996 459 127
BmgBI CAC'GTC
Cuts at: 1318 2590 1318
Size: 1272 4039
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3042 4000 442
Size: 416 53 83 399 24 1625 958 1753
Fragments arranged by size:
1753 1625 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5311
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2444 3971 1239
Size: 1205 1527 2579
Fragments arranged by size:
2579 1527 1205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3436 4301 4599 3436
Size: 865 298 4148
Fragments arranged by size:
4148 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2384 3974 882
Size: 617 885 1590 2219
Fragments arranged by size:
2219 1590 885 617
BsaAI yAC'GTr
Cuts at: 493 1204 2377 3102 493
Size: 711 1173 725 2702
Fragments arranged by size:
2702 1173 725 711
BsaBI GATnn'nnATC
Cuts at: 5305 5305
Size: 5311
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2358 3118 3500 4135
Size: 53 83 186 936 825 760 382 635
Cuts at: 4135 275
Size: 1451
Fragments arranged by size:
1451 936 825 760 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3744 4142 4416 4563 4974 3744
Size: 398 274 147 411 4081
Fragments arranged by size:
4081 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5086
Fragments arranged by size:
5086 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2620 1186
Size: 1434 3877
BseYI C'CCAG_C
Cuts at: 2059 4514 5010 2059
Size: 2455 496 2360
Fragments arranged by size:
2455 2360 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4738
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2335 2761 3522 4550 1540
Size: 275 186 334 426 761 1028 2301
Fragments arranged by size:
2301 1028 761 426 334 275 186
BsiHKAI G_wGCw'C
Cuts at: 2376 3372 3457 4528 2376
Size: 996 85 1071 3159
Fragments arranged by size:
3159 1071 996 85
BsmI GAATG_Cn'
Cuts at: 2822 2915 2822
Size: 93 5218
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2177 2249 2376 2630 3372 3457 4528
Size: 399 387 72 127 254 742 85 1071
Cuts at: 4528 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 387 254 127 85
72
BspEI T'CCGG_A
Cuts at: 4974 4974
Size: 5311
BspHI T'CATG_A
Cuts at: 3200 4930 3200
Size: 1730 3581
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2287 2666 844
Size: 1443 379 3489
Fragments arranged by size:
3489 1443 379
BsrBI CCG'CTC
Cuts at: 1394 1823 2428 2463 3198 1394
Size: 429 605 35 735 3507
Fragments arranged by size:
3507 735 605 429 35
BsrDI GCAATG_nn'
Cuts at: 65 1248 3806 3980 65
Size: 1183 2558 174 1396
Fragments arranged by size:
2558 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2085 3955 4142 1700
Size: 110 275 1870 187 2869
Fragments arranged by size:
2869 1870 275 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5311
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5311
BssSI C'ACGA_G
Cuts at: 3371 4383 3371
Size: 1012 4299
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5311
BstBI TT'CG_AA
Cuts at: 4115 4115
Size: 5311
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5311
BstXI CCAn_nnnn'nTGG
Cuts at: 2312 2312
Size: 5311
BstYI r'GATC_y
Cuts at: 1972 2286 2538 2641 3008 3393 3410 4862 4948
Size: 314 252 103 367 385 17 1452 86
Cuts at: 4948 4960 5306 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 314 252 103
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2253 513
Size: 552 1188 3571
Fragments arranged by size:
3571 1188 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4572
Fragments arranged by size:
4572 683 56
BtsI GCAGTG_nn'
Cuts at: 2225 2827 3621 3641 2225
Size: 602 794 20 3895
Fragments arranged by size:
3895 794 602 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5311
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5276
DraI TTT'AAA
Cuts at: 2615 2615
Size: 5311
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2324 4318 809
Size: 507 1008 1994 1802
Fragments arranged by size:
1994 1802 1008 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2332 2758 3647
Size: 54 1425 50 275 18 502 426 889
Cuts at: 3647 5276 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 502 426 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2332 2758 1537
Size: 275 520 426 4090
Fragments arranged by size:
4090 520 426 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3241 1353
Size: 1888 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3898 4272 4418 387
Size: 1064 138 2309 374 146 1280
Fragments arranged by size:
2309 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2444 3374 3971 4758 5026 1239
Size: 393 812 930 597 787 268 1524
Fragments arranged by size:
1524 930 812 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2528 2528
Size: 5311
EcoO109I rG'GnC_Cy
Cuts at: 2151 2326 2151
Size: 175 5136
EcoRI G'AATT_C
Cuts at: 2620 2998 2620
Size: 378 4933
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2578 2578
Size: 5311
FspI TGC'GCA
Cuts at: 3817 3817
Size: 5311
HaeII r_GCGC'y
Cuts at: 1678 2361 4458 1678
Size: 683 2097 2531
Fragments arranged by size:
2531 2097 683
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3952 3984 4026 4058 1373
Size: 32 135 32 2380 32 42 32 2626
Fragments arranged by size:
2626 2380 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2655 2901 669
Size: 988 281 717 246 3079
Fragments arranged by size:
3079 988 717 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5311
HpaI GTT'AAC
Cuts at: 2901 2901
Size: 5311
KasI G'GCGC_C
Cuts at: 2357 2357
Size: 5311
KpnI G_GTAC'C
Cuts at: 2636 2636
Size: 5311
MfeI C'AATT_G
Cuts at: 2910 2910
Size: 5311
MluI A'CGCG_T
Cuts at: 5187 5187
Size: 5311
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4425 4609 1305
Size: 3120 184 2007
Fragments arranged by size:
3120 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3489
Fragments arranged by size:
3489 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2252 3269 3628 3787 518
Size: 648 1086 1017 359 159 2042
Fragments arranged by size:
2042 1086 1017 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2051 2440 2506 2727 3404 4552 4797 1606
Size: 445 389 66 221 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 445 389 245 221 66
NaeI GCC'GGC
Cuts at: 2087 2087
Size: 5311
NarI GG'CG_CC
Cuts at: 2358 2358
Size: 5311
NcoI C'CATG_G
Cuts at: 513 1065 2253 513
Size: 552 1188 3571
Fragments arranged by size:
3571 1188 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5311
NgoMIV G'CCGG_C
Cuts at: 2085 2085
Size: 5311
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5311
NotI GC'GGCC_GC
Cuts at: 2758 2758
Size: 5311
NspI r_CATG'y
Cuts at: 1132 1229 2234 2669 4214 1132
Size: 97 1005 435 1545 2229
Fragments arranged by size:
2229 1545 1005 435 97
PasI CC'CwG_GG
Cuts at: 1348 2015 2242 1348
Size: 667 227 4417
Fragments arranged by size:
4417 667 227
PciI A'CATG_T
Cuts at: 4210 4210
Size: 5311
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2593 1108
Size: 1485 3826
PfoI T'CCnGG_A
Cuts at: 2511 2592 2511
Size: 81 5230
PmeI GTTT'AAAC
Cuts at: 2615 2615
Size: 5311
PmlI CAC'GTG
Cuts at: 2377 2377
Size: 5311
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3380 3412 4893 4925 3380
Size: 32 1481 32 3766
Fragments arranged by size:
3766 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2151 2326 2151
Size: 175 5136
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5311
PsiI TTA'TAA
Cuts at: 2881 2881
Size: 5311
PstI C_TGCA'G
Cuts at: 830 2196 2578 2663 830
Size: 1366 382 85 3478
Fragments arranged by size:
3478 1366 382 85
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5311
PvuII CAG'CTG
Cuts at: 2051 2440 2051
Size: 389 4922
SalI G'TCGA_C
Cuts at: 1936 2653 1936
Size: 717 4594
SbfI CC_TGCA'GG
Cuts at: 2196 2663 2196
Size: 467 4844
ScaI AGT'ACT
Cuts at: 3559 5271 3559
Size: 1712 3599
SfcI C'TryA_G
Cuts at: 826 1045 2192 2574 2659 3794 4475 4666 826
Size: 219 1147 382 85 1135 681 191 1471
Fragments arranged by size:
1471 1147 1135 681 382 219 191 85
SfiI GGCCn_nnn'nGGCC
Cuts at: 2086 2086
Size: 5311
SfoI GGC'GCC
Cuts at: 2359 2359
Size: 5311
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5311
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5311
SmaI CCC'GGG
Cuts at: 1872 2638 1872
Size: 766 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3415 4316 4578 820
Size: 197 926 1472 901 262 1553
Fragments arranged by size:
1553 1472 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3102 493
Size: 2609 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5311
SphI G_CATG'C
Cuts at: 1132 2669 1132
Size: 1537 3774
SspI AAT'ATT
Cuts at: 5 52 3235 5
Size: 47 3183 2081
Fragments arranged by size:
3183 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5311
StyI C'CwwG_G
Cuts at: 513 1065 2118 2253 2750 513
Size: 552 1053 135 497 3074
Fragments arranged by size:
3074 1053 552 497 135
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2408 2473 3338 3355 3508 3693 4138
Size: 1255 828 65 865 17 153 185 445
Cuts at: 4138 325
Size: 1498
Fragments arranged by size:
1498 1255 865 828 445 185 153 65
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3557 5269 96
Size: 274 80 33 51 1443 1580 1712 138
Fragments arranged by size:
1712 1580 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5271 5303 5271
Size: 32 5279
Tth111I GACn'n_nGTC
Cuts at: 1316 2483 1316
Size: 1167 4144
XbaI T'CTAG_A
Cuts at: 2647 2647
Size: 5311
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2230 2230
Size: 5311
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5311
XmaI C'CCGG_G
Cuts at: 1870 2636 1870
Size: 766 4545
XmnI GAAnn'nnTTC
Cuts at: 3440 3440
Size: 5311
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3119 276
Size: 53 83 186 936 1585 2468
Fragments arranged by size:
2468 1585 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI NruI NsiI PacI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SrfI
SwaI