Restriction map for
pFN21AE1694
(Circular) (Six-base) MAPSORT of: pFN21AE1694.seq Check: 9612 from: 1 to: 5308
pFN21AE1694 5308 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3118 278
Size: 53 83 186 936 1582 2468
Fragments arranged by size:
2468 1582 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2651 1937
Size: 714 4594
Acc65I G'GTAC_C
Cuts at: 2629 2629
Size: 5308
AclI AA'CG_TT
Cuts at: 1563 3435 3808 1563
Size: 1872 373 3063
Fragments arranged by size:
3063 1872 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3371 4755 5023 1632
Size: 1739 1384 268 1917
Fragments arranged by size:
1917 1739 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5111
AflIII A'CryG_T
Cuts at: 1203 2322 4207 5184 1203
Size: 1119 1885 977 1327
Fragments arranged by size:
1885 1327 1119 977
AgeI A'CCGG_T
Cuts at: 4139 4139
Size: 5308
AhdI GACnn_n'nnGTC
Cuts at: 4037 4037
Size: 5308
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5308
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5308
AlwNI CAG_nnn'CTG
Cuts at: 3051 4623 3051
Size: 1572 3736
ApaLI G'TGCA_C
Cuts at: 3365 4521 3365
Size: 1156 4152
ApoI r'AATT_y
Cuts at: 1493 2617 2843 2995 5104 1493
Size: 1124 226 152 2109 1697
Fragments arranged by size:
2109 1697 1124 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3862 160
Size: 872 2830 1606
Fragments arranged by size:
2830 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5308
AvaI C'yCGr_G
Cuts at: 1870 1943 2633 1870
Size: 73 690 4545
Fragments arranged by size:
4545 690 73
BamHI G'GATC_C
Cuts at: 2048 2099 2638 3005 2048
Size: 51 539 367 4351
Fragments arranged by size:
4351 539 367 51
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2629 4084 618
Size: 325 217 627 842 1455 1842
Fragments arranged by size:
1842 1455 842 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2576 2627 1391
Size: 1185 51 4072
Fragments arranged by size:
4072 1185 51
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4337
BbvCI CC'TCA_GC
Cuts at: 2400 2400
Size: 5308
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3499 3533 1903
Size: 34 1562 34 3678
Fragments arranged by size:
3678 1562 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3200 4410 3200
Size: 1210 4098
BclI T'GATC_A
Cuts at: 1548 2031 1548
Size: 483 4825
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3919 136
Size: 107 122 71 631 605 2247 1525
Fragments arranged by size:
2247 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5303 5303
Size: 5308
BlpI GC'TnA_GC
Cuts at: 2277 2575 2725 2277
Size: 298 150 4860
Fragments arranged by size:
4860 298 150
Bme1580I G_kGCm'C
Cuts at: 1790 3369 4525 1790
Size: 1579 1156 2573
Fragments arranged by size:
2573 1579 1156
BmgBI CAC'GTC
Cuts at: 1318 2296 1318
Size: 978 4330
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2588 3039 3997
Size: 416 53 83 399 24 1171 451 958
Cuts at: 3997 442
Size: 1753
Fragments arranged by size:
1753 1171 958 451 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5308
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3968 1239
Size: 2729 2579
Bpu10I CC'TnA_GC
Cuts at: 2400 2400
Size: 5308
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3433 4298 4596 3433
Size: 865 298 4145
Fragments arranged by size:
4145 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3971 882
Size: 617 2472 2219
Fragments arranged by size:
2472 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2166 2323 3099 493
Size: 711 962 157 776 2702
Fragments arranged by size:
2702 962 776 711 157
BsaBI GATnn'nnATC
Cuts at: 5302 5302
Size: 5308
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2310 3115 3497 4132
Size: 53 83 186 936 777 805 382 635
Cuts at: 4132 275
Size: 1451
Fragments arranged by size:
1451 936 805 777 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3741 4139 4413 4560 4971 3741
Size: 398 274 147 411 4078
Fragments arranged by size:
4078 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5083
Fragments arranged by size:
5083 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2414 1186
Size: 1228 4080
BseYI C'CCAG_C
Cuts at: 2070 2341 2545 4511 5007 2070
Size: 271 204 1966 496 2371
Fragments arranged by size:
2371 1966 496 271 204
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2267 2347 1163
Size: 573 531 80 4124
Fragments arranged by size:
4124 573 531 80
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2758 3519 4547 1540
Size: 275 186 757 761 1028 2301
Fragments arranged by size:
2301 1028 761 757 275 186
BsiHKAI G_wGCw'C
Cuts at: 2059 2576 3369 3454 4525 2059
Size: 517 793 85 1071 2842
Fragments arranged by size:
2842 1071 793 517 85
BsmI GAATG_Cn'
Cuts at: 2819 2912 2819
Size: 93 5215
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2059 2576 2627 3369 3454 4525 1391
Size: 399 269 517 51 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 517 399 269 85 51
BspEI T'CCGG_A
Cuts at: 4971 4971
Size: 5308
BspHI T'CATG_A
Cuts at: 3197 4927 3197
Size: 1730 3578
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2663 844
Size: 1819 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3195 1394
Size: 429 1372 3507
Fragments arranged by size:
3507 1372 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3803 3977 65
Size: 1183 2555 174 1396
Fragments arranged by size:
2555 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2533 3952 4139 1700
Size: 110 723 1419 187 2869
Fragments arranged by size:
2869 1419 723 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5308
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5308
BssSI C'ACGA_G
Cuts at: 3368 4380 3368
Size: 1012 4296
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5308
BstBI TT'CG_AA
Cuts at: 4112 4112
Size: 5308
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5308
BstYI r'GATC_y
Cuts at: 1972 2048 2099 2638 3005 3390 3407 4859 4945
Size: 76 51 539 367 385 17 1452 86
Cuts at: 4945 4957 5303 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 539 385 367 346 86 76
51 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2469 513
Size: 552 939 465 3352
Fragments arranged by size:
3352 939 552 465
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4569
Fragments arranged by size:
4569 683 56
BtsI GCAGTG_nn'
Cuts at: 2824 3618 3638 2824
Size: 794 20 4494
Fragments arranged by size:
4494 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5308
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5273
DraI TTT'AAA
Cuts at: 2612 2612
Size: 5308
DraIII CAC_nnn'GTG
Cuts at: 2567 2567
Size: 5308
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4315 809
Size: 507 2999 1802
Fragments arranged by size:
2999 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2148 2466 2755
Size: 54 1425 50 275 18 318 318 289
Cuts at: 2755 3644 5273 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 318 318 289 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2755 1537
Size: 275 943 4090
Fragments arranged by size:
4090 943 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3238 1353
Size: 1885 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3895 4269 4415 387
Size: 1064 138 2306 374 146 1280
Fragments arranged by size:
2306 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2574 2574
Size: 5308
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3371 3968 4755 5023 1239
Size: 393 1739 597 787 268 1524
Fragments arranged by size:
1739 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2403 2403
Size: 5308
EcoRI G'AATT_C
Cuts at: 2617 2995 2617
Size: 378 4930
FspI TGC'GCA
Cuts at: 3814 3814
Size: 5308
HaeII r_GCGC'y
Cuts at: 1678 2552 4455 1678
Size: 874 1903 2531
Fragments arranged by size:
2531 1903 874
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3949 3981 4023 4055 1373
Size: 32 135 32 2377 32 42 32 2626
Fragments arranged by size:
2626 2377 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2652 2898 669
Size: 988 281 714 246 3079
Fragments arranged by size:
3079 988 714 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5308
HpaI GTT'AAC
Cuts at: 2898 2898
Size: 5308
KpnI G_GTAC'C
Cuts at: 2633 2633
Size: 5308
MfeI C'AATT_G
Cuts at: 2907 2907
Size: 5308
MluI A'CGCG_T
Cuts at: 5184 5184
Size: 5308
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4422 4606 1305
Size: 3117 184 2007
Fragments arranged by size:
3117 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2150 10
Size: 54 1425 343 318 3168
Fragments arranged by size:
3168 1425 343 318 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2321 2563 3266 3625 3784 518
Size: 648 1155 242 703 359 159 2042
Fragments arranged by size:
2042 1155 703 648 359 242 159
MspA1I CmG'CkG
Cuts at: 1606 2530 2724 3401 4549 4794 1606
Size: 924 194 677 1148 245 2120
Fragments arranged by size:
2120 1148 924 677 245 194
NaeI GCC'GGC
Cuts at: 2535 2535
Size: 5308
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3817
Fragments arranged by size:
3817 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5308
NgoMIV G'CCGG_C
Cuts at: 2533 2533
Size: 5308
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5308
NotI GC'GGCC_GC
Cuts at: 2755 2755
Size: 5308
NspI r_CATG'y
Cuts at: 1132 1229 2666 4211 1132
Size: 97 1437 1545 2229
Fragments arranged by size:
2229 1545 1437 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5308
PciI A'CATG_T
Cuts at: 4207 4207
Size: 5308
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2567 1108
Size: 902 557 3849
Fragments arranged by size:
3849 902 557
PfoI T'CCnGG_A
Cuts at: 2044 2289 2044
Size: 245 5063
PmeI GTTT'AAAC
Cuts at: 2612 2612
Size: 5308
PmlI CAC'GTG
Cuts at: 2166 2166
Size: 5308
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3377 3409 4890 4922 3377
Size: 32 1481 32 3763
Fragments arranged by size:
3763 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5308
PsiI TTA'TAA
Cuts at: 2878 2878
Size: 5308
PstI C_TGCA'G
Cuts at: 830 2660 830
Size: 1830 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5308
PvuII CAG'CTG
Cuts at: 2530 2530
Size: 5308
SacI G_AGCT'C
Cuts at: 2576 2576
Size: 5308
SalI G'TCGA_C
Cuts at: 1936 2650 1936
Size: 714 4594
SbfI CC_TGCA'GG
Cuts at: 2660 2660
Size: 5308
ScaI AGT'ACT
Cuts at: 3556 5268 3556
Size: 1712 3596
SfcI C'TryA_G
Cuts at: 826 1045 2656 3791 4472 4663 826
Size: 219 1611 1135 681 191 1471
Fragments arranged by size:
1611 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5308
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5308
SmaI CCC'GGG
Cuts at: 1872 2635 1872
Size: 763 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3412 4313 4575 820
Size: 197 926 1469 901 262 1553
Fragments arranged by size:
1553 1469 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3099 493
Size: 2606 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5308
SphI G_CATG'C
Cuts at: 1132 2666 1132
Size: 1534 3774
SspI AAT'ATT
Cuts at: 5 52 3232 5
Size: 47 3180 2081
Fragments arranged by size:
3180 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5308
StyI C'CwwG_G
Cuts at: 513 1065 2004 2137 2347 2747 513
Size: 552 939 133 210 400 3074
Fragments arranged by size:
3074 939 552 400 210 133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2053 3335 3352 3505 3690 4135 325
Size: 1255 473 1282 17 153 185 445 1498
Fragments arranged by size:
1498 1282 1255 473 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3554 5266 96
Size: 274 80 33 51 1443 1577 1712 138
Fragments arranged by size:
1712 1577 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5268 5300 5268
Size: 32 5276
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5308
XbaI T'CTAG_A
Cuts at: 2644 2644
Size: 5308
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5308
XmaI C'CCGG_G
Cuts at: 1870 2633 1870
Size: 763 4545
XmnI GAAnn'nnTTC
Cuts at: 3437 3437
Size: 5308
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3116 276
Size: 53 83 186 936 1582 2468
Fragments arranged by size:
2468 1582 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoO109I
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI