Restriction map for
pFN21AE1692
(Circular) (Six-base) MAPSORT of: pFN21AE1692.seq Check: 2191 from: 1 to: 5305
pFN21AE1692 5305 bp
With 182 enzymes: SgfI *
March 28, 2012 12:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2343 3115 278
Size: 53 83 186 936 807 772 2468
Fragments arranged by size:
2468 936 807 772 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2648 1937
Size: 711 4594
Acc65I G'GTAC_C
Cuts at: 2626 2626
Size: 5305
AclI AA'CG_TT
Cuts at: 1563 3432 3805 1563
Size: 1869 373 3063
Fragments arranged by size:
3063 1869 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2321 3368 4752 5020 1632
Size: 689 1047 1384 268 1917
Fragments arranged by size:
1917 1384 1047 689 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5108
AflIII A'CryG_T
Cuts at: 1203 4204 5181 1203
Size: 3001 977 1327
Fragments arranged by size:
3001 1327 977
AgeI A'CCGG_T
Cuts at: 4136 4136
Size: 5305
AhdI GACnn_n'nnGTC
Cuts at: 4034 4034
Size: 5305
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5305
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5305
AlwNI CAG_nnn'CTG
Cuts at: 2066 3048 4620 2066
Size: 982 1572 2751
Fragments arranged by size:
2751 1572 982
ApaI G_GGCC'C
Cuts at: 2424 2424
Size: 5305
ApaLI G'TGCA_C
Cuts at: 3362 4518 3362
Size: 1156 4149
ApoI r'AATT_y
Cuts at: 1493 2614 2840 2992 5101 1493
Size: 1121 226 152 2109 1697
Fragments arranged by size:
2109 1697 1121 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3859 160
Size: 872 2827 1606
Fragments arranged by size:
2827 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5305
AvaI C'yCGr_G
Cuts at: 1870 1943 2225 2430 2630 1870
Size: 73 282 205 200 4545
Fragments arranged by size:
4545 282 205 200 73
BamHI G'GATC_C
Cuts at: 2635 3002 2635
Size: 367 4938
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2626 4081 618
Size: 325 217 627 839 1455 1842
Fragments arranged by size:
1842 1455 839 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2424 2624 1391
Size: 1033 200 4072
Fragments arranged by size:
4072 1033 200
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2212 928
Size: 971 313 4021
Fragments arranged by size:
4021 971 313
BbvCI CC'TCA_GC
Cuts at: 2406 2406
Size: 5305
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3496 3530 1903
Size: 34 1559 34 3678
Fragments arranged by size:
3678 1559 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2408 3197 4407 2408
Size: 789 1210 3306
Fragments arranged by size:
3306 1210 789
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5305
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2112 2279 3916
Size: 107 122 71 631 605 440 167 1637
Cuts at: 3916 136
Size: 1525
Fragments arranged by size:
1637 1525 631 605 440 167 122 107
71
BglII A'GATC_T
Cuts at: 5300 5300
Size: 5305
BlpI GC'TnA_GC
Cuts at: 2283 2722 2283
Size: 439 4866
Bme1580I G_kGCm'C
Cuts at: 1790 2424 3366 4522 1790
Size: 634 942 1156 2573
Fragments arranged by size:
2573 1156 942 634
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5305
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2103 3036 3994
Size: 416 53 83 399 24 686 933 958
Cuts at: 3994 442
Size: 1753
Fragments arranged by size:
1753 958 933 686 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2147 1056
Size: 1091 4214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2221 2234 2330 2354 2372 2447 2519 3965
Size: 982 13 96 24 18 75 72 1446
Cuts at: 3965 1239
Size: 2579
Fragments arranged by size:
2579 1446 982 96 75 72 24 18
13
Bpu10I CC'TnA_GC
Cuts at: 2406 2406
Size: 5305
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2171 3430 4295 4593 2171
Size: 1259 865 298 2883
Fragments arranged by size:
2883 1259 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2535 3968 882
Size: 617 1036 1433 2219
Fragments arranged by size:
2219 1433 1036 617
BsaAI yAC'GTr
Cuts at: 493 1204 3096 493
Size: 711 1892 2702
Fragments arranged by size:
2702 1892 711
BsaBI GATnn'nnATC
Cuts at: 5299 5299
Size: 5305
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2169 2340 3112 3494
Size: 53 83 186 936 636 171 772 382
Cuts at: 3494 4129 275
Size: 635 1451
Fragments arranged by size:
1451 936 772 636 635 382 186 171
83 53
BsaWI w'CCGG_w
Cuts at: 3738 4136 4410 4557 4968 3738
Size: 398 274 147 411 4075
Fragments arranged by size:
4075 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2363 2393 1178
Size: 30 165 30 960 30 4090
Fragments arranged by size:
4090 960 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2522 1186
Size: 1336 3969
BseYI C'CCAG_C
Cuts at: 2326 2382 4508 5004 2326
Size: 56 2126 496 2627
Fragments arranged by size:
2627 2126 496 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2351 2438 1163
Size: 573 615 87 4030
Fragments arranged by size:
4030 615 573 87
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2755 3516 4544 1540
Size: 275 186 754 761 1028 2301
Fragments arranged by size:
2301 1028 761 754 275 186
BsiHKAI G_wGCw'C
Cuts at: 3366 3451 4522 3366
Size: 85 1071 4149
Fragments arranged by size:
4149 1071 85
BsmI GAATG_Cn'
Cuts at: 2816 2909 2816
Size: 93 5212
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2424 2624 3366 3451 4522 1391
Size: 399 634 200 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 634 399 200 85
BspEI T'CCGG_A
Cuts at: 4968 4968
Size: 5305
BspHI T'CATG_A
Cuts at: 3194 4924 3194
Size: 1730 3575
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2106 2660 844
Size: 1262 554 3489
Fragments arranged by size:
3489 1262 554
BsrBI CCG'CTC
Cuts at: 1394 1823 3192 1394
Size: 429 1369 3507
Fragments arranged by size:
3507 1369 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3800 3974 65
Size: 1183 2552 174 1396
Fragments arranged by size:
2552 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2457 3949 4136 1700
Size: 110 647 1492 187 2869
Fragments arranged by size:
2869 1492 647 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5305
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5305
BssSI C'ACGA_G
Cuts at: 3365 4377 3365
Size: 1012 4293
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5305
BstBI TT'CG_AA
Cuts at: 4109 4109
Size: 5305
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5305
BstYI r'GATC_y
Cuts at: 1972 2635 3002 3387 3404 4856 4942 4954 5300
Size: 663 367 385 17 1452 86 12 346
Cuts at: 5300 1972
Size: 1977
Fragments arranged by size:
1977 1452 663 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2240 2240
Size: 5305
BtgI C'CryG_G
Cuts at: 513 1065 2004 2022 2055 513
Size: 552 939 18 33 3763
Fragments arranged by size:
3763 939 552 33 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4566
Fragments arranged by size:
4566 683 56
BtsI GCAGTG_nn'
Cuts at: 2821 3615 3635 2821
Size: 794 20 4491
Fragments arranged by size:
4491 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5305
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5270
DraI TTT'AAA
Cuts at: 2609 2609
Size: 5305
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4312 809
Size: 507 2996 1802
Fragments arranged by size:
2996 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2052 2556 2752
Size: 54 1425 50 275 18 222 504 196
Cuts at: 2752 3641 5270 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 504 275 222 196 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2752 1537
Size: 275 940 4090
Fragments arranged by size:
4090 940 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2172 2528 3235 1353
Size: 819 356 707 3423
Fragments arranged by size:
3423 819 707 356
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2516 3892 4266 4412 387
Size: 1064 138 927 1376 374 146 1280
Fragments arranged by size:
1376 1280 1064 927 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2221 2234 2321 2330 2354 2372 2447
Size: 393 589 13 87 9 24 18 75
Cuts at: 2447 2519 3368 3965 4752 5020 1239
Size: 72 849 597 787 268 1524
Fragments arranged by size:
1524 849 787 597 589 393 268 87
75 72 24 18 13 9
EcoO109I rG'GnC_Cy
Cuts at: 2063 2236 2305 2420 2427 2470 2063
Size: 173 69 115 7 43 4898
Fragments arranged by size:
4898 173 115 69 43 7
EcoRI G'AATT_C
Cuts at: 2614 2992 2614
Size: 378 4927
FspI TGC'GCA
Cuts at: 3811 3811
Size: 5305
HaeII r_GCGC'y
Cuts at: 1678 2266 4452 1678
Size: 588 2186 2531
Fragments arranged by size:
2531 2186 588
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3946 3978 4020 4052 1373
Size: 32 135 32 2374 32 42 32 2626
Fragments arranged by size:
2626 2374 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2586 2649 2895 669
Size: 988 281 648 63 246 3079
Fragments arranged by size:
3079 988 648 281 246 63
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5305
HpaI GTT'AAC
Cuts at: 2895 2895
Size: 5305
KpnI G_GTAC'C
Cuts at: 2630 2630
Size: 5305
MfeI C'AATT_G
Cuts at: 2904 2904
Size: 5305
MluI A'CGCG_T
Cuts at: 5181 5181
Size: 5305
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4419 4603 1305
Size: 3114 184 2007
Fragments arranged by size:
3114 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3483
Fragments arranged by size:
3483 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3263 3622 3781 518
Size: 648 2097 359 159 2042
Fragments arranged by size:
2097 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2318 2489 2721 3398 4546 4791 1606
Size: 712 171 232 677 1148 245 2120
Fragments arranged by size:
2120 1148 712 677 245 232 171
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3814
Fragments arranged by size:
3814 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5305
NheI G'CTAG_C
Cuts at: 1052 2143 1052
Size: 1091 4214
NotI GC'GGCC_GC
Cuts at: 2752 2752
Size: 5305
NspI r_CATG'y
Cuts at: 1132 1229 2663 4208 1132
Size: 97 1434 1545 2229
Fragments arranged by size:
2229 1545 1434 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5305
PciI A'CATG_T
Cuts at: 4204 4204
Size: 5305
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5305
PfoI T'CCnGG_A
Cuts at: 2307 2463 2307
Size: 156 5149
PmeI GTTT'AAAC
Cuts at: 2609 2609
Size: 5305
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3374 3406 4887 4919 3374
Size: 32 1481 32 3760
Fragments arranged by size:
3760 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2236 2305 2236
Size: 69 5236
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5305
PsiI TTA'TAA
Cuts at: 2875 2875
Size: 5305
PspOMI G'GGCC_C
Cuts at: 2420 2420
Size: 5305
PstI C_TGCA'G
Cuts at: 830 2014 2657 830
Size: 1184 643 3478
Fragments arranged by size:
3478 1184 643
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5305
SalI G'TCGA_C
Cuts at: 1936 2647 1936
Size: 711 4594
SbfI CC_TGCA'GG
Cuts at: 2657 2657
Size: 5305
ScaI AGT'ACT
Cuts at: 3553 5265 3553
Size: 1712 3593
SfcI C'TryA_G
Cuts at: 826 1045 2010 2653 3788 4469 4660 826
Size: 219 965 643 1135 681 191 1471
Fragments arranged by size:
1471 1135 965 681 643 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5305
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5305
SmaI CCC'GGG
Cuts at: 1872 2227 2432 2632 1872
Size: 355 205 200 4545
Fragments arranged by size:
4545 355 205 200
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2186 3409 4310 4572 820
Size: 197 926 243 1223 901 262 1553
Fragments arranged by size:
1553 1223 926 901 262 243 197
SnaBI TAC'GTA
Cuts at: 493 3096 493
Size: 2603 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5305
SphI G_CATG'C
Cuts at: 1132 2663 1132
Size: 1531 3774
SspI AAT'ATT
Cuts at: 5 52 3229 5
Size: 47 3177 2081
Fragments arranged by size:
3177 2081 47
StuI AGG'CCT
Cuts at: 1517 2393 1517
Size: 876 4429
StyI C'CwwG_G
Cuts at: 513 1065 2004 2172 2274 2744 513
Size: 552 939 168 102 470 3074
Fragments arranged by size:
3074 939 552 470 168 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3332 3349 3502 3687 4132 325
Size: 1255 1752 17 153 185 445 1498
Fragments arranged by size:
1752 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3551 5263 96
Size: 274 80 33 51 1443 1574 1712 138
Fragments arranged by size:
1712 1574 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2363 2395 2582 2614 5265 5297 2363
Size: 32 187 32 2651 32 2371
Fragments arranged by size:
2651 2371 187 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5305
XbaI T'CTAG_A
Cuts at: 2641 2641
Size: 5305
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2170 2170
Size: 5305
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5305
XmaI C'CCGG_G
Cuts at: 1870 2225 2430 2630 1870
Size: 355 205 200 4545
Fragments arranged by size:
4545 355 205 200
XmnI GAAnn'nnTTC
Cuts at: 3434 3434
Size: 5305
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2341 3113 276
Size: 53 83 186 936 807 772 2468
Fragments arranged by size:
2468 936 807 772 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI