Restriction map for
pFN21AE1691
(Circular) (Six-base) MAPSORT of: pFN21AE1691.seq Check: 6811 from: 1 to: 5305
pFN21AE1691 5305 bp
With 182 enzymes: SgfI *
March 28, 2012 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2214 2263 2214
Size: 49 5256
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2370 3115 278
Size: 53 83 186 936 834 745 2468
Fragments arranged by size:
2468 936 834 745 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2648 1937
Size: 711 4594
Acc65I G'GTAC_C
Cuts at: 2626 2626
Size: 5305
AclI AA'CG_TT
Cuts at: 1563 3432 3805 1563
Size: 1869 373 3063
Fragments arranged by size:
3063 1869 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2234 2342 3368 4752 5020 1632
Size: 602 108 1026 1384 268 1917
Fragments arranged by size:
1917 1384 1026 602 268 108
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5108
AflIII A'CryG_T
Cuts at: 1203 4204 5181 1203
Size: 3001 977 1327
Fragments arranged by size:
3001 1327 977
AgeI A'CCGG_T
Cuts at: 4136 4136
Size: 5305
AhdI GACnn_n'nnGTC
Cuts at: 4034 4034
Size: 5305
AleI CACnn'nnGTG
Cuts at: 1166 2036 1166
Size: 870 4435
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2054 2140 1184
Size: 870 86 4349
Fragments arranged by size:
4349 870 86
AlwNI CAG_nnn'CTG
Cuts at: 2084 2164 2253 3048 4620 2084
Size: 80 89 795 1572 2769
Fragments arranged by size:
2769 1572 795 89 80
ApaI G_GGCC'C
Cuts at: 2451 2451
Size: 5305
ApaLI G'TGCA_C
Cuts at: 3362 4518 3362
Size: 1156 4149
ApoI r'AATT_y
Cuts at: 1493 2614 2840 2992 5101 1493
Size: 1121 226 152 2109 1697
Fragments arranged by size:
2109 1697 1121 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3859 160
Size: 872 2827 1606
Fragments arranged by size:
2827 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5305
AvaI C'yCGr_G
Cuts at: 1870 1943 2457 2525 2630 1870
Size: 73 514 68 105 4545
Fragments arranged by size:
4545 514 105 73 68
BamHI G'GATC_C
Cuts at: 2635 3002 2635
Size: 367 4938
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2226 2626 4081 618
Size: 325 217 627 439 400 1455 1842
Fragments arranged by size:
1842 1455 627 439 400 325 217
BanII G_rGCy'C
Cuts at: 1391 2107 2451 2624 1391
Size: 716 344 173 4072
Fragments arranged by size:
4072 716 344 173
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4334
BbvCI CC'TCA_GC
Cuts at: 2433 2433
Size: 5305
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3496 3530 1903
Size: 34 1559 34 3678
Fragments arranged by size:
3678 1559 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2435 3197 4407 2435
Size: 762 1210 3333
Fragments arranged by size:
3333 1210 762
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5305
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3916 136
Size: 107 122 71 631 605 2244 1525
Fragments arranged by size:
2244 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5300 5300
Size: 5305
BlpI GC'TnA_GC
Cuts at: 2304 2722 2304
Size: 418 4887
Bme1580I G_kGCm'C
Cuts at: 1790 2451 3366 4522 1790
Size: 661 915 1156 2573
Fragments arranged by size:
2573 1156 915 661
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5305
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2031 2131 2404
Size: 416 53 83 399 24 614 100 273
Cuts at: 2404 3036 3994 442
Size: 632 958 1753
Fragments arranged by size:
1753 958 632 614 416 399 273 100
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5305
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2094 2351 2474 3965 1239
Size: 855 257 123 1491 2579
Fragments arranged by size:
2579 1491 855 257 123
Bpu10I CC'TnA_GC
Cuts at: 2433 2433
Size: 5305
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3430 4295 4593 3430
Size: 865 298 4142
Fragments arranged by size:
4142 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2562 3968 882
Size: 617 1063 1406 2219
Fragments arranged by size:
2219 1406 1063 617
BsaAI yAC'GTr
Cuts at: 493 1204 3096 493
Size: 711 1892 2702
Fragments arranged by size:
2702 1892 711
BsaBI GATnn'nnATC
Cuts at: 5299 5299
Size: 5305
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2367 3112 3494 4129
Size: 53 83 186 936 834 745 382 635
Cuts at: 4129 275
Size: 1451
Fragments arranged by size:
1451 936 834 745 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3738 4136 4410 4557 4968 3738
Size: 398 274 147 411 4075
Fragments arranged by size:
4075 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5080
Fragments arranged by size:
5080 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2549 1186
Size: 1363 3942
BseYI C'CCAG_C
Cuts at: 2353 4508 5004 2353
Size: 2155 496 2654
Fragments arranged by size:
2654 2155 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2014 2378 1163
Size: 573 278 364 4090
Fragments arranged by size:
4090 573 364 278
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2755 3516 4544 1540
Size: 275 186 754 761 1028 2301
Fragments arranged by size:
2301 1028 761 754 275 186
BsiHKAI G_wGCw'C
Cuts at: 3366 3451 4522 3366
Size: 85 1071 4149
Fragments arranged by size:
4149 1071 85
BsmI GAATG_Cn'
Cuts at: 2816 2909 2816
Size: 93 5212
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2107 2451 2624 3366 3451 4522 1391
Size: 399 317 344 173 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 344 317 173 85
BspEI T'CCGG_A
Cuts at: 4968 4968
Size: 5305
BspHI T'CATG_A
Cuts at: 3194 4924 3194
Size: 1730 3575
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2214 2263 2660 844
Size: 1370 49 397 3489
Fragments arranged by size:
3489 1370 397 49
BsrBI CCG'CTC
Cuts at: 1394 1823 3192 1394
Size: 429 1369 3507
Fragments arranged by size:
3507 1369 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3800 3974 65
Size: 1183 2552 174 1396
Fragments arranged by size:
2552 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2484 3949 4136 1700
Size: 110 674 1465 187 2869
Fragments arranged by size:
2869 1465 674 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5305
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5305
BssSI C'ACGA_G
Cuts at: 3365 4377 3365
Size: 1012 4293
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5305
BstBI TT'CG_AA
Cuts at: 4109 4109
Size: 5305
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5305
BstXI CCAn_nnnn'nTGG
Cuts at: 2296 2296
Size: 5305
BstYI r'GATC_y
Cuts at: 1972 2635 3002 3387 3404 4856 4942 4954 5300
Size: 663 367 385 17 1452 86 12 346
Cuts at: 5300 1972
Size: 1977
Fragments arranged by size:
1977 1452 663 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2261 2261
Size: 5305
BtgI C'CryG_G
Cuts at: 513 1065 2098 2289 2439 513
Size: 552 1033 191 150 3379
Fragments arranged by size:
3379 1033 552 191 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4566
Fragments arranged by size:
4566 683 56
BtsI GCAGTG_nn'
Cuts at: 2064 2821 3615 3635 2064
Size: 757 794 20 3734
Fragments arranged by size:
3734 794 757 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5305
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5270
DraI TTT'AAA
Cuts at: 2609 2609
Size: 5305
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2600 4312 809
Size: 507 1284 1712 1802
Fragments arranged by size:
1802 1712 1284 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2052 2752 3641
Size: 54 1425 50 275 18 222 700 889
Cuts at: 3641 5270 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 700 275 222 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2752 1537
Size: 275 940 4090
Fragments arranged by size:
4090 940 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2193 2246 2555 3235 1353
Size: 840 53 309 680 3423
Fragments arranged by size:
3423 840 680 309 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2543 3892 4266 4412 387
Size: 1064 138 954 1349 374 146 1280
Fragments arranged by size:
1349 1280 1064 954 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2094 2234 2342 2351 2474 3368 3965
Size: 393 462 140 108 9 123 894 597
Cuts at: 3965 4752 5020 1239
Size: 787 268 1524
Fragments arranged by size:
1524 894 787 597 462 393 268 140
123 108 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2176 2176
Size: 5305
EcoO109I rG'GnC_Cy
Cuts at: 2250 2257 2447 2454 2497 2593 2250
Size: 7 190 7 43 96 4962
Fragments arranged by size:
4962 190 96 43 7 7
EcoRI G'AATT_C
Cuts at: 2614 2992 2614
Size: 378 4927
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2222 2222
Size: 5305
FspI TGC'GCA
Cuts at: 3811 3811
Size: 5305
HaeII r_GCGC'y
Cuts at: 1678 2287 4452 1678
Size: 609 2165 2531
Fragments arranged by size:
2531 2165 609
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3946 3978 4020 4052 1373
Size: 32 135 32 2374 32 42 32 2626
Fragments arranged by size:
2626 2374 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2649 2895 669
Size: 988 281 711 246 3079
Fragments arranged by size:
3079 988 711 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5305
HpaI GTT'AAC
Cuts at: 2895 2895
Size: 5305
KpnI G_GTAC'C
Cuts at: 2630 2630
Size: 5305
MfeI C'AATT_G
Cuts at: 2904 2904
Size: 5305
MluI A'CGCG_T
Cuts at: 5181 5181
Size: 5305
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4419 4603 1305
Size: 3114 184 2007
Fragments arranged by size:
3114 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3483
Fragments arranged by size:
3483 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2036 3263 3622 3781 518
Size: 648 870 1227 359 159 2042
Fragments arranged by size:
2042 1227 870 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2269 2342 2721 3398 4546 4791 1606
Size: 663 73 379 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 663 379 245 73
NcoI C'CATG_G
Cuts at: 513 1065 2289 513
Size: 552 1224 3529
Fragments arranged by size:
3529 1224 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5305
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5305
NotI GC'GGCC_GC
Cuts at: 2752 2752
Size: 5305
NspI r_CATG'y
Cuts at: 1132 1229 2663 4208 1132
Size: 97 1434 1545 2229
Fragments arranged by size:
2229 1545 1434 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5305
PciI A'CATG_T
Cuts at: 4204 4204
Size: 5305
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2253 1108
Size: 1145 4160
PfoI T'CCnGG_A
Cuts at: 2109 2490 2109
Size: 381 4924
PmeI GTTT'AAAC
Cuts at: 2609 2609
Size: 5305
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3374 3406 4887 4919 3374
Size: 32 1481 32 3760
Fragments arranged by size:
3760 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2250 2257 2454 2593 2250
Size: 7 197 139 4962
Fragments arranged by size:
4962 197 139 7
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5305
PsiI TTA'TAA
Cuts at: 2875 2875
Size: 5305
PspOMI G'GGCC_C
Cuts at: 2447 2447
Size: 5305
PspXI vC'TCGA_Gb
Cuts at: 2525 2525
Size: 5305
PstI C_TGCA'G
Cuts at: 830 2169 2225 2274 2657 830
Size: 1339 56 49 383 3478
Fragments arranged by size:
3478 1339 383 56 49
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5305
PvuII CAG'CTG
Cuts at: 2269 2269
Size: 5305
SalI G'TCGA_C
Cuts at: 1936 2647 1936
Size: 711 4594
SbfI CC_TGCA'GG
Cuts at: 2657 2657
Size: 5305
ScaI AGT'ACT
Cuts at: 3553 5265 3553
Size: 1712 3593
SfcI C'TryA_G
Cuts at: 826 1045 2165 2221 2270 2653 3788 4469 4660
Size: 219 1120 56 49 383 1135 681 191
Cuts at: 4660 826
Size: 1471
Fragments arranged by size:
1471 1135 1120 681 383 219 191 56
49
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5305
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5305
SmaI CCC'GGG
Cuts at: 1872 2459 2632 1872
Size: 587 173 4545
Fragments arranged by size:
4545 587 173
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2525 3409 4310 4572 820
Size: 197 926 582 884 901 262 1553
Fragments arranged by size:
1553 926 901 884 582 262 197
SnaBI TAC'GTA
Cuts at: 493 3096 493
Size: 2603 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5305
SphI G_CATG'C
Cuts at: 1132 2663 1132
Size: 1531 3774
SspI AAT'ATT
Cuts at: 5 52 3229 5
Size: 47 3177 2081
Fragments arranged by size:
3177 2081 47
StuI AGG'CCT
Cuts at: 1517 2171 2531 1517
Size: 654 360 4291
Fragments arranged by size:
4291 654 360
StyI C'CwwG_G
Cuts at: 513 1065 2289 2744 513
Size: 552 1224 455 3074
Fragments arranged by size:
3074 1224 552 455
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3332 3349 3502 3687 4132 325
Size: 1255 1752 17 153 185 445 1498
Fragments arranged by size:
1752 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3551 5263 96
Size: 274 80 33 51 1443 1574 1712 138
Fragments arranged by size:
1712 1574 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2210 2242 5265 5297 2210
Size: 32 3023 32 2218
Fragments arranged by size:
3023 2218 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5305
XbaI T'CTAG_A
Cuts at: 2641 2641
Size: 5305
XhoI C'TCGA_G
Cuts at: 1943 2525 1943
Size: 582 4723
XmaI C'CCGG_G
Cuts at: 1870 2457 2630 1870
Size: 587 173 4545
Fragments arranged by size:
4545 587 173
XmnI GAAnn'nnTTC
Cuts at: 3434 3434
Size: 5305
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2368 3113 276
Size: 53 83 186 936 834 745 2468
Fragments arranged by size:
2468 936 834 745 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI